UniProtKB - P09759 (EPHB1_RAT)
Ephrin type-B receptor 1
Ephb1
Functioni
Catalytic activityi
- EC:2.7.10.1PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 651 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 744 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 625 – 633 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- axon guidance receptor activity Source: RGD
- protein-containing complex binding Source: RGD
- protein tyrosine kinase activity Source: UniProtKB
- transmembrane-ephrin receptor activity Source: UniProtKB
- transmembrane receptor protein tyrosine kinase activity Source: GO_Central
GO - Biological processi
- angiogenesis Source: UniProtKB
- axon guidance Source: UniProtKB
- camera-type eye morphogenesis Source: RGD
- cell chemotaxis Source: UniProtKB
- cell-substrate adhesion Source: UniProtKB
- central nervous system projection neuron axonogenesis Source: UniProtKB
- cranial nerve development Source: RGD
- dendritic spine development Source: MGI
- dendritic spine morphogenesis Source: UniProtKB
- detection of temperature stimulus involved in sensory perception of pain Source: UniProtKB
- ephrin receptor signaling pathway Source: UniProtKB
- establishment of cell polarity Source: UniProtKB
- immunological synapse formation Source: MGI
- modulation of chemical synaptic transmission Source: RGD
- multicellular organism development Source: GO_Central
- negative regulation of satellite cell differentiation Source: UniProtKB
- negative regulation of skeletal muscle satellite cell proliferation Source: UniProtKB
- neural precursor cell proliferation Source: UniProtKB
- neurogenesis Source: UniProtKB
- optic nerve morphogenesis Source: RGD
- positive regulation of kinase activity Source: GO_Central
- positive regulation of synapse assembly Source: UniProtKB
- protein autophosphorylation Source: RGD
- regulation of ERK1 and ERK2 cascade Source: UniProtKB
- regulation of JNK cascade Source: UniProtKB
- regulation of neuron death Source: RGD
- retinal ganglion cell axon guidance Source: UniProtKB
- skeletal muscle satellite cell activation Source: UniProtKB
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Kinase, Receptor, Transferase, Tyrosine-protein kinase |
Biological process | Cell adhesion, Neurogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 5301 |
Reactomei | R-RNO-2682334, EPH-Ephrin signaling R-RNO-3928662, EPHB-mediated forward signaling R-RNO-3928664, Ephrin signaling R-RNO-3928665, EPH-ephrin mediated repulsion of cells |
Names & Taxonomyi
Protein namesi | Recommended name: Ephrin type-B receptor 1 (EC:2.7.10.1)Alternative name(s): ELK Tyrosine-protein kinase receptor EPH-2 |
Gene namesi | Name:Ephb1 Synonyms:Elk, Epth2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2556, Ephb1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein By similarity
Endosome
- Early endosome membrane By similarity
Other locations
- dendrite By similarity
Endosome
- early endosome membrane Source: UniProtKB
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Other locations
- axon Source: RGD
- cytoplasm Source: RGD
- dendrite Source: UniProtKB-SubCell
- filopodium tip Source: RGD
- glutamatergic synapse Source: RGD
- membrane Source: RGD
- membrane raft Source: RGD
- neuron projection Source: GO_Central
- receptor complex Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 18 – 540 | ExtracellularSequence analysisAdd BLAST | 523 | |
Transmembranei | 541 – 563 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 564 – 984 | CytoplasmicSequence analysisAdd BLAST | 421 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
ChainiPRO_0000016825 | 18 – 984 | Ephrin type-B receptor 1Add BLAST | 967 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 334 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 426 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 480 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 600 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 928 | Phosphotyrosine; by autocatalysisBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P09759 |
PRIDEi | P09759 |
PTM databases
GlyGeni | P09759, 3 sites |
iPTMneti | P09759 |
PhosphoSitePlusi | P09759 |
SwissPalmi | P09759 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000007865, Expressed in brain and 21 other tissues |
Genevisiblei | P09759, RN |
Interactioni
Subunit structurei
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.
Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex.
Interacts with PICK1.
Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion. The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (residues within the catalytic domain) with GRB2 (via SH2 domain).
Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration.
Interacts with CBL; regulates receptor degradation through ubiquitination.
Interacts with ACP1 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 246515, 2 interactors |
CORUMi | P09759 |
DIPi | DIP-138N |
STRINGi | 10116.ENSRNOP00000010634 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 201 | Eph LBDPROSITE-ProRule annotationAdd BLAST | 183 | |
Domaini | 322 – 432 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 111 | |
Domaini | 433 – 528 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 619 – 882 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 264 | |
Domaini | 911 – 975 | SAMPROSITE-ProRule annotationAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 982 – 984 | PDZ-bindingSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 183 – 319 | Cys-richAdd BLAST | 137 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0196, Eukaryota |
GeneTreei | ENSGT00940000155297 |
HOGENOMi | CLU_000288_141_4_1 |
InParanoidi | P09759 |
OMAi | MACKACP |
OrthoDBi | 933071at2759 |
PhylomeDBi | P09759 |
TreeFami | TF315608 |
Family and domain databases
CDDi | cd10476, EphR_LBD_B1, 1 hit cd00063, FN3, 2 hits cd09551, SAM_EPH-B1, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR034231, EphB1_rcpt_lig-bd IPR042819, EphB1_SAM IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00790, RECEPTOR_TYR_KIN_V_1, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MALDCLLLFL LASAVAAMEE TLMDTRTATA ELGWTANPAS GWEEVSGYDE
60 70 80 90 100
NLNTIRTYQV CNVFEPNQNN WLLTTFINRR GAHRIYTEMR FTVRDCSSLP
110 120 130 140 150
NVPGSCKETF NLYYYETDSV IATKKSAFWS EAPYLKVDTI AADESFSQVD
160 170 180 190 200
FGGRLMKVNT EVRSFGPLTR NGFYLAFQDY GACMSLLSVR VFFKKCPSIV
210 220 230 240 250
QNFAVFPETM TGAESTSLVI ARGTCIPNAE EVDVPIKLYC NGDGEWMVPI
260 270 280 290 300
GRCTCKAGYE PENSVACKAC PAGTFKASQE AEGCSHCPSN SRSPSEASPI
310 320 330 340 350
CTCRTGYYRA DFDPPEVACT SVPSGPRNVI SIVNETSIIL EWHPPRETGG
360 370 380 390 400
RDDVTYNIIC KKCRADRRSC SRCDDNVEFV PRQLGLTECR VSISSLWAHT
410 420 430 440 450
PYTFDIQAIN GVSSKSPFPP QHVSVNITTN QAAPSTVPIM HQVSATMRSI
460 470 480 490 500
TLSWPQPEQP NGIILDYEIR YYEKEHNEFN SSMARSQTNT ARIDGLRPGM
510 520 530 540 550
VYVVQVRART VAGYGKFSGK MCFQTLTDDD YKSELREQLP LIAGSAAAGV
560 570 580 590 600
VFVVSLVAIS IVCSRKRAYS KEAVYSDKLQ HYSTGRGSPG MKIYIDPFTY
610 620 630 640 650
EDPNEAVREF AKEIDVSFVK IEEVIGAGEF GEVYKGRLKL PGKREIYVAI
660 670 680 690 700
KTLKAGYSEK QRRDFLSEAS IMGQFDHPNI IRLEGVVTKS RPVMIITEFM
710 720 730 740 750
ENGALDSFLR QNDGQFTVIQ LVGMLRGIAA GMKYLSEMNY VHRDLAARNI
760 770 780 790 800
LVNSNLVCKV SDFGLSRYLQ DDTSDPTYTS SLGGKIPVRW TAPEAIAYRK
810 820 830 840 850
FTSASDVWSY GIVMWEVMSF GERPYWDMSN QDVINAIEQD YRLPPPMDCP
860 870 880 890 900
AALHQLMLDC WQKDRNSRPR FAEIVNTLDK MIRNPASLKT VATITAVPSQ
910 920 930 940 950
PLLDRSIPDF TAFTTVDDWL SAIKMVQYRD SFLTAGFTSL QLVTQMTSED
960 970 980
LLRIGVTLAG HQKKILSSIH SMRVQMNQSP SVMA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59814 mRNA No translation available. X13411 mRNA Translation: CAA31777.1 |
PIRi | A39753 |
RefSeqi | NP_001097998.1, NM_001104528.1 XP_017450939.1, XM_017595450.1 |
Genome annotation databases
Ensembli | ENSRNOT00000010634; ENSRNOP00000010634; ENSRNOG00000007865 |
GeneIDi | 24338 |
KEGGi | rno:24338 |
UCSCi | RGD:2556, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59814 mRNA No translation available. X13411 mRNA Translation: CAA31777.1 |
PIRi | A39753 |
RefSeqi | NP_001097998.1, NM_001104528.1 XP_017450939.1, XM_017595450.1 |
3D structure databases
SMRi | P09759 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246515, 2 interactors |
CORUMi | P09759 |
DIPi | DIP-138N |
STRINGi | 10116.ENSRNOP00000010634 |
Chemistry databases
GuidetoPHARMACOLOGYi | 1830 |
PTM databases
GlyGeni | P09759, 3 sites |
iPTMneti | P09759 |
PhosphoSitePlusi | P09759 |
SwissPalmi | P09759 |
Proteomic databases
PaxDbi | P09759 |
PRIDEi | P09759 |
Genome annotation databases
Ensembli | ENSRNOT00000010634; ENSRNOP00000010634; ENSRNOG00000007865 |
GeneIDi | 24338 |
KEGGi | rno:24338 |
UCSCi | RGD:2556, rat |
Organism-specific databases
CTDi | 2047 |
RGDi | 2556, Ephb1 |
Phylogenomic databases
eggNOGi | KOG0196, Eukaryota |
GeneTreei | ENSGT00940000155297 |
HOGENOMi | CLU_000288_141_4_1 |
InParanoidi | P09759 |
OMAi | MACKACP |
OrthoDBi | 933071at2759 |
PhylomeDBi | P09759 |
TreeFami | TF315608 |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 5301 |
Reactomei | R-RNO-2682334, EPH-Ephrin signaling R-RNO-3928662, EPHB-mediated forward signaling R-RNO-3928664, Ephrin signaling R-RNO-3928665, EPH-ephrin mediated repulsion of cells |
Miscellaneous databases
PROi | PR:P09759 |
Gene expression databases
Bgeei | ENSRNOG00000007865, Expressed in brain and 21 other tissues |
Genevisiblei | P09759, RN |
Family and domain databases
CDDi | cd10476, EphR_LBD_B1, 1 hit cd00063, FN3, 2 hits cd09551, SAM_EPH-B1, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR034231, EphB1_rcpt_lig-bd IPR042819, EphB1_SAM IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00790, RECEPTOR_TYR_KIN_V_1, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EPHB1_RAT | |
Accessioni | P09759Primary (citable) accession number: P09759 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | December 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 207 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families