UniProtKB - P09733 (TBA1_YEAST)
Protein
Tubulin alpha-1 chain
Gene
TUB1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Miscellaneous
Present with 5590 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 143 – 149 | GTPSequence analysis | 7 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: GO_Central
- structural constituent of cytoskeleton Source: GO_Central
GO - Biological processi
- homologous chromosome segregation Source: SGD
- microtubule-based process Source: GO_Central
- microtubule cytoskeleton organization Source: GO_Central
- mitotic cell cycle Source: GO_Central
- mitotic sister chromatid segregation Source: SGD
- nuclear division Source: GO_Central
- nuclear migration along microtubule Source: SGD
- nuclear migration by microtubule mediated pushing forces Source: GO_Central
Keywordsi
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-114608, Platelet degranulation R-SCE-5617833, Cilium Assembly |
Names & Taxonomyi
Protein namesi | Recommended name: Tubulin alpha-1 chain |
Gene namesi | Name:TUB1 Ordered Locus Names:YML085C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004550, TUB1 |
VEuPathDBi | FungiDB:YML085C |
Subcellular locationi
Cytoskeleton
Cytoskeleton
- cytoplasmic microtubule Source: SGD
- kinetochore microtubule Source: SGD
- microtubule Source: GO_Central
- nuclear microtubule Source: SGD
- polar microtubule Source: SGD
- spindle Source: GO_Central
- tubulin complex Source: SGD
Nucleus
- nuclear microtubule Source: SGD
- nuclear periphery Source: SGD
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048239 | 1 – 447 | Tubulin alpha-1 chainAdd BLAST | 447 |
Proteomic databases
MaxQBi | P09733 |
PaxDbi | P09733 |
PRIDEi | P09733 |
PTM databases
iPTMneti | P09733 |
SwissPalmi | P09733 |
Interactioni
Subunit structurei
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Protein-protein interaction databases
BioGRIDi | 35056, 150 interactors |
ComplexPortali | CPX-1424, Tubulin alpha-beta heterodimeric complex, TUB1 variant |
DIPi | DIP-854N |
IntActi | P09733, 315 interactors |
MINTi | P09733 |
STRINGi | 4932.YML085C |
Miscellaneous databases
RNActi | P09733, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09733 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the tubulin family.Curated
Phylogenomic databases
eggNOGi | KOG1376, Eukaryota |
GeneTreei | ENSGT00940000164588 |
HOGENOMi | CLU_015718_0_0_1 |
InParanoidi | P09733 |
OMAi | VDNEACY |
Family and domain databases
Gene3Di | 1.10.287.600, 1 hit 3.30.1330.20, 1 hit 3.40.50.1440, 1 hit |
InterProi | View protein in InterPro IPR002452, Alpha_tubulin IPR008280, Tub_FtsZ_C IPR000217, Tubulin IPR018316, Tubulin/FtsZ_2-layer-sand-dom IPR037103, Tubulin/FtsZ_C_sf IPR036525, Tubulin/FtsZ_GTPase_sf IPR023123, Tubulin_C IPR017975, Tubulin_CS IPR003008, Tubulin_FtsZ_GTPase |
PANTHERi | PTHR11588, PTHR11588, 1 hit |
Pfami | View protein in Pfam PF00091, Tubulin, 1 hit PF03953, Tubulin_C, 1 hit |
PRINTSi | PR01162, ALPHATUBULIN PR01161, TUBULIN |
SMARTi | View protein in SMART SM00864, Tubulin, 1 hit SM00865, Tubulin_C, 1 hit |
SUPFAMi | SSF52490, SSF52490, 1 hit SSF55307, SSF55307, 1 hit |
PROSITEi | View protein in PROSITE PS00227, TUBULIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P09733-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREVISINVG QAGCQIGNAC WELYSLEHGI KPDGHLEDGL SKPKGGEEGF
60 70 80 90 100
STFFHETGYG KFVPRAIYVD LEPNVIDEVR NGPYKDLFHP EQLISGKEDA
110 120 130 140 150
ANNYARGHYT VGREILGDVL DRIRKLADQC DGLQGFLFTH SLGGGTGSGL
160 170 180 190 200
GSLLLEELSA EYGKKSKLEF AVYPAPQVST SVVEPYNTVL TTHTTLEHAD
210 220 230 240 250
CTFMVDNEAI YDMCKRNLDI PRPSFANLNN LIAQVVSSVT ASLRFDGSLN
260 270 280 290 300
VDLNEFQTNL VPYPRIHFPL VSYSPVLSKS KAFHESNSVS EITNACFEPG
310 320 330 340 350
NQMVKCDPRD GKYMATCLLY RGDVVTRDVQ RAVEQVKNKK TVQLVDWCPT
360 370 380 390 400
GFKIGICYEP PTATPNSQLA TVDRAVCMLS NTTSIAEAWK RIDRKFDLMY
410 420 430 440
AKRAFVHWYV GEGMEEGEFT EAREDLAALE RDYIEVGADS YAEEEEF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | I → L in AAA35180 (PubMed:3025610).Curated | 1 | |
Sequence conflicti | 327 | R → G in AAA35180 (PubMed:3025610).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28429 mRNA Translation: AAA35180.1 Z46660 Genomic DNA Translation: CAA86653.1 BK006946 Genomic DNA Translation: DAA09812.1 |
PIRi | S50871 |
RefSeqi | NP_013625.1, NM_001182444.1 |
Genome annotation databases
EnsemblFungii | YML085C_mRNA; YML085C; YML085C |
GeneIDi | 854889 |
KEGGi | sce:YML085C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28429 mRNA Translation: AAA35180.1 Z46660 Genomic DNA Translation: CAA86653.1 BK006946 Genomic DNA Translation: DAA09812.1 |
PIRi | S50871 |
RefSeqi | NP_013625.1, NM_001182444.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4FFB | X-ray | 2.88 | A | 1-447 | [»] | |
4U3J | X-ray | 2.81 | A | 1-447 | [»] | |
5W3F | electron microscopy | 3.70 | A | 1-447 | [»] | |
5W3H | electron microscopy | 4.00 | A | 1-447 | [»] | |
5W3J | electron microscopy | 4.00 | A | 1-447 | [»] | |
SMRi | P09733 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35056, 150 interactors |
ComplexPortali | CPX-1424, Tubulin alpha-beta heterodimeric complex, TUB1 variant |
DIPi | DIP-854N |
IntActi | P09733, 315 interactors |
MINTi | P09733 |
STRINGi | 4932.YML085C |
PTM databases
iPTMneti | P09733 |
SwissPalmi | P09733 |
Proteomic databases
MaxQBi | P09733 |
PaxDbi | P09733 |
PRIDEi | P09733 |
Genome annotation databases
EnsemblFungii | YML085C_mRNA; YML085C; YML085C |
GeneIDi | 854889 |
KEGGi | sce:YML085C |
Organism-specific databases
SGDi | S000004550, TUB1 |
VEuPathDBi | FungiDB:YML085C |
Phylogenomic databases
eggNOGi | KOG1376, Eukaryota |
GeneTreei | ENSGT00940000164588 |
HOGENOMi | CLU_015718_0_0_1 |
InParanoidi | P09733 |
OMAi | VDNEACY |
Enzyme and pathway databases
Reactomei | R-SCE-114608, Platelet degranulation R-SCE-5617833, Cilium Assembly |
Miscellaneous databases
PROi | PR:P09733 |
RNActi | P09733, protein |
Family and domain databases
Gene3Di | 1.10.287.600, 1 hit 3.30.1330.20, 1 hit 3.40.50.1440, 1 hit |
InterProi | View protein in InterPro IPR002452, Alpha_tubulin IPR008280, Tub_FtsZ_C IPR000217, Tubulin IPR018316, Tubulin/FtsZ_2-layer-sand-dom IPR037103, Tubulin/FtsZ_C_sf IPR036525, Tubulin/FtsZ_GTPase_sf IPR023123, Tubulin_C IPR017975, Tubulin_CS IPR003008, Tubulin_FtsZ_GTPase |
PANTHERi | PTHR11588, PTHR11588, 1 hit |
Pfami | View protein in Pfam PF00091, Tubulin, 1 hit PF03953, Tubulin_C, 1 hit |
PRINTSi | PR01162, ALPHATUBULIN PR01161, TUBULIN |
SMARTi | View protein in SMART SM00864, Tubulin, 1 hit SM00865, Tubulin_C, 1 hit |
SUPFAMi | SSF52490, SSF52490, 1 hit SSF55307, SSF55307, 1 hit |
PROSITEi | View protein in PROSITE PS00227, TUBULIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBA1_YEAST | |
Accessioni | P09733Primary (citable) accession number: P09733 Secondary accession number(s): D6W0J8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 189 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families