UniProtKB - P09661 (RU2A_HUMAN)
U2 small nuclear ribonucleoprotein A'
SNRPA1
Functioni
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346, PubMed:27035939).
Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA (PubMed:9716128, PubMed:27035939).
6 PublicationsGO - Molecular functioni
- RNA binding Source: UniProtKB
- U2 snRNA binding Source: GO_Central
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- RNA splicing Source: ProtInc
- spermatogenesis Source: UniProtKB
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | P09661 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-8950505, Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation |
SignaLinki | P09661 |
Names & Taxonomyi
Protein namesi | Recommended name: U2 small nuclear ribonucleoprotein A'Short name: U2 snRNP A'2 Publications |
Gene namesi | Name:SNRPA1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11152, SNRPA1 |
MIMi | 603521, gene |
neXtProti | NX_P09661 |
VEuPathDBi | HostDB:ENSG00000131876 |
Subcellular locationi
Nucleus
- Nucleus 4 Publications
Nucleus
- catalytic step 2 spliceosome Source: UniProtKB
- nuclear body Source: HPA
- nuclear speck Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- small nuclear ribonucleoprotein complex Source: ProtInc
- spliceosomal complex Source: HGNC-UCL
- U2 snRNP Source: GO_Central
- U2-type catalytic step 2 spliceosome Source: UniProtKB
- U2-type precatalytic spliceosome Source: UniProtKB
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 15 | Y → D: Results in defective spliceosome assembly. 1 Publication | 1 | |
Mutagenesisi | 27 | R → Q: No change in spliceosome assembly. 1 Publication | 1 | |
Mutagenesisi | 68 | T → K: No change in spliceosome assembly. 1 Publication | 1 | |
Mutagenesisi | 72 | N → K: No change in spliceosome assembly. 1 Publication | 1 | |
Mutagenesisi | 73 | N → K: No change in spliceosome assembly. 1 Publication | 1 | |
Mutagenesisi | 92 | E → V: Results in defective spliceosome assembly. 1 Publication | 1 | |
Mutagenesisi | 148 | Q → R: Results in defective spliceosome assembly. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6627 |
OpenTargetsi | ENSG00000131876 |
PharmGKBi | PA35994 |
Miscellaneous databases
Pharosi | P09661, Tbio |
Genetic variation databases
BioMutai | SNRPA1 |
DMDMi | 31077165 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000074173 | 2 – 255 | U2 small nuclear ribonucleoprotein A'Add BLAST | 254 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 172 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 172 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 178 | PhosphoserineCombined sources | 1 | |
Modified residuei | 197 | PhosphoserineCombined sources | 1 | |
Cross-linki | 221 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 236 | PhosphoserineCombined sources | 1 | |
Modified residuei | 255 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P09661 |
jPOSTi | P09661 |
MassIVEi | P09661 |
MaxQBi | P09661 |
PaxDbi | P09661 |
PeptideAtlasi | P09661 |
PRIDEi | P09661 |
ProteomicsDBi | 52261 |
PTM databases
GlyGeni | P09661, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P09661 |
MetOSitei | P09661 |
PhosphoSitePlusi | P09661 |
SwissPalmi | P09661 |
Expressioni
Gene expression databases
Bgeei | ENSG00000131876, Expressed in small intestine Peyer's patch and 116 other tissues |
ExpressionAtlasi | P09661, baseline and differential |
Genevisiblei | P09661, HS |
Organism-specific databases
HPAi | ENSG00000131876, Low tissue specificity |
Interactioni
Subunit structurei
Identified in the spliceosome B complex (PubMed:28781166). Identified in the spliceosome C complex (PubMed:11991638, PubMed:28502770, PubMed:28076346).
Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2 (PubMed:9531537).
Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B (PubMed:10339552). Contributes to the binding of stem loop IV of U2 snRNA with SNRPB2 (PubMed:9716128, PubMed:27035939).
8 PublicationsBinary interactionsi
P09661
Protein-protein interaction databases
BioGRIDi | 112511, 235 interactors |
CORUMi | P09661 |
DIPi | DIP-625N |
IntActi | P09661, 90 interactors |
MINTi | P09661 |
STRINGi | 9606.ENSP00000254193 |
Miscellaneous databases
RNActi | P09661, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P09661 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09661 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 20 – 41 | LRR 1Add BLAST | 22 | |
Repeati | 43 – 64 | LRR 2Add BLAST | 22 | |
Repeati | 65 – 86 | LRR 3Add BLAST | 22 | |
Repeati | 89 – 110 | LRR 4Add BLAST | 22 | |
Domaini | 123 – 161 | LRRCTAdd BLAST | 39 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 174 – 201 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 222 – 255 | DisorderedSequence analysisAdd BLAST | 34 |
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG1644, Eukaryota |
GeneTreei | ENSGT00940000153289 |
HOGENOMi | CLU_061027_0_1_1 |
OMAi | PNYREYM |
OrthoDBi | 1447031at2759 |
PhylomeDBi | P09661 |
TreeFami | TF313776 |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR044640, RU2A IPR003603, U2A'_phosphoprotein32A_C |
PANTHERi | PTHR10552, PTHR10552, 1 hit |
SMARTi | View protein in SMART SM00446, LRRcap, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 4 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS
60 70 80 90 100
DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPCL TELILTNNSL
110 120 130 140 150
VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV
160 170 180 190 200
KLKERQEAEK MFKGKRGAQL AKDIARRSKT FNPGAGLPTD KKKGGPSPGD
210 220 230 240 250
VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD DGEEEMEEDT
VTNGS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YKK0 | H0YKK0_HUMAN | Small nuclear ribonucleoprotein pol... | SNRPA1 hCG_1992004 | 154 | Annotation score: | ||
H0YMA0 | H0YMA0_HUMAN | U2 small nuclear ribonucleoprotein ... | SNRPA1 | 142 | Annotation score: | ||
H0YLR3 | H0YLR3_HUMAN | U2 small nuclear ribonucleoprotein ... | SNRPA1 | 89 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 193 | K → R in CAA31838 (PubMed:2928112).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13482 mRNA Translation: CAA31838.1 AK312200 mRNA Translation: BAG35133.1 CH471101 Genomic DNA Translation: EAX02300.1 BC022816 mRNA Translation: AAH22816.1 BC071717 mRNA Translation: AAH71717.1 |
CCDSi | CCDS10391.1 |
PIRi | S03616 |
RefSeqi | NP_003081.2, NM_003090.3 |
Genome annotation databases
Ensembli | ENST00000254193; ENSP00000254193; ENSG00000131876 |
GeneIDi | 6627 |
KEGGi | hsa:6627 |
MANE-Selecti | ENST00000254193.11; ENSP00000254193.6; NM_003090.4; NP_003081.2 |
UCSCi | uc002bww.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13482 mRNA Translation: CAA31838.1 AK312200 mRNA Translation: BAG35133.1 CH471101 Genomic DNA Translation: EAX02300.1 BC022816 mRNA Translation: AAH22816.1 BC071717 mRNA Translation: AAH71717.1 |
CCDSi | CCDS10391.1 |
PIRi | S03616 |
RefSeqi | NP_003081.2, NM_003090.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A9N | X-ray | 2.38 | A/C | 1-176 | [»] | |
5MQF | electron microscopy | 5.90 | W | 1-255 | [»] | |
5O9Z | electron microscopy | 4.50 | z | 1-255 | [»] | |
5XJC | electron microscopy | 3.60 | o | 1-255 | [»] | |
5YZG | electron microscopy | 4.10 | o | 1-255 | [»] | |
5Z56 | electron microscopy | 5.10 | o | 1-255 | [»] | |
5Z57 | electron microscopy | 6.50 | o | 1-255 | [»] | |
5Z58 | electron microscopy | 4.90 | o | 1-255 | [»] | |
6AH0 | electron microscopy | 5.70 | o | 1-255 | [»] | |
6AHD | electron microscopy | 3.80 | o | 1-255 | [»] | |
6FF7 | electron microscopy | 4.50 | W | 1-255 | [»] | |
6ICZ | electron microscopy | 3.00 | o | 1-255 | [»] | |
6ID0 | electron microscopy | 2.90 | o | 1-255 | [»] | |
6ID1 | electron microscopy | 2.86 | o | 1-255 | [»] | |
6QDV | electron microscopy | 3.30 | W | 2-162 | [»] | |
6QX9 | electron microscopy | 3.28 | 2A | 1-255 | [»] | |
6Y53 | electron microscopy | 7.10 | a | 1-255 | [»] | |
6Y5Q | electron microscopy | 7.10 | a | 1-255 | [»] | |
7A5P | electron microscopy | 5.00 | W | 1-255 | [»] | |
7ABG | electron microscopy | 7.80 | W | 1-255 | [»] | |
7ABI | electron microscopy | 8.00 | W | 1-255 | [»] | |
SMRi | P09661 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112511, 235 interactors |
CORUMi | P09661 |
DIPi | DIP-625N |
IntActi | P09661, 90 interactors |
MINTi | P09661 |
STRINGi | 9606.ENSP00000254193 |
PTM databases
GlyGeni | P09661, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P09661 |
MetOSitei | P09661 |
PhosphoSitePlusi | P09661 |
SwissPalmi | P09661 |
Genetic variation databases
BioMutai | SNRPA1 |
DMDMi | 31077165 |
Proteomic databases
EPDi | P09661 |
jPOSTi | P09661 |
MassIVEi | P09661 |
MaxQBi | P09661 |
PaxDbi | P09661 |
PeptideAtlasi | P09661 |
PRIDEi | P09661 |
ProteomicsDBi | 52261 |
Protocols and materials databases
Antibodypediai | 29301, 112 antibodies from 26 providers |
DNASUi | 6627 |
Genome annotation databases
Ensembli | ENST00000254193; ENSP00000254193; ENSG00000131876 |
GeneIDi | 6627 |
KEGGi | hsa:6627 |
MANE-Selecti | ENST00000254193.11; ENSP00000254193.6; NM_003090.4; NP_003081.2 |
UCSCi | uc002bww.4, human |
Organism-specific databases
CTDi | 6627 |
DisGeNETi | 6627 |
GeneCardsi | SNRPA1 |
HGNCi | HGNC:11152, SNRPA1 |
HPAi | ENSG00000131876, Low tissue specificity |
MIMi | 603521, gene |
neXtProti | NX_P09661 |
OpenTargetsi | ENSG00000131876 |
PharmGKBi | PA35994 |
VEuPathDBi | HostDB:ENSG00000131876 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1644, Eukaryota |
GeneTreei | ENSGT00940000153289 |
HOGENOMi | CLU_061027_0_1_1 |
OMAi | PNYREYM |
OrthoDBi | 1447031at2759 |
PhylomeDBi | P09661 |
TreeFami | TF313776 |
Enzyme and pathway databases
PathwayCommonsi | P09661 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-8950505, Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation |
SignaLinki | P09661 |
Miscellaneous databases
BioGRID-ORCSi | 6627, 778 hits in 982 CRISPR screens |
ChiTaRSi | SNRPA1, human |
EvolutionaryTracei | P09661 |
GeneWikii | SNRPA1 |
GenomeRNAii | 6627 |
Pharosi | P09661, Tbio |
PROi | PR:P09661 |
RNActi | P09661, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000131876, Expressed in small intestine Peyer's patch and 116 other tissues |
ExpressionAtlasi | P09661, baseline and differential |
Genevisiblei | P09661, HS |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR044640, RU2A IPR003603, U2A'_phosphoprotein32A_C |
PANTHERi | PTHR10552, PTHR10552, 1 hit |
SMARTi | View protein in SMART SM00446, LRRcap, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | RU2A_HUMAN | |
Accessioni | P09661Primary (citable) accession number: P09661 Secondary accession number(s): B2R5I6, Q8TBD2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | May 23, 2003 | |
Last modified: | February 23, 2022 | |
This is version 211 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families