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Protein

Mast cell protease 1

Gene

Mcpt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. May participate in generating perivascular beta-protein which ultimately aggregates into amyloid-beta deposits.1 Publication

Catalytic activityi

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay systemBy similarity1
Active sitei109Charge relay systemBy similarity1
Active sitei202Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.149

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 1 (EC:3.4.21.39)
Short name:
rMCP-1
Alternative name(s):
Chymase
Chymotrypsin-like protease
Short name:
CLIP protein
Mast cell protease I
Short name:
rMCP-I
Gene namesi
Name:Mcpt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi3062 Mcpt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002743919 – 20Activation peptide3 Publications2
ChainiPRO_000002744021 – 260Mast cell protease 1Add BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Disulfide bondi143 ↔ 208PROSITE-ProRule annotation
Disulfide bondi174 ↔ 187PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP09650
PRIDEiP09650

Expressioni

Gene expression databases

BgeeiENSRNOG00000053494 Expressed in 9 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP09650 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028012

Structurei

3D structure databases

ProteinModelPortaliP09650
SMRiP09650
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 244Peptidase S1PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118895
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP09650
KOiK01329
OMAiNLQICVG
OrthoDBiEOG091G0G5F
PhylomeDBiP09650
TreeFamiTF333630

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P09650-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQALLFLMAL LLPSGAGAEE IIGGVESRPH SRPYMAHLEI TTERGYKATC
60 70 80 90 100
GGFLVTRQFV MTAAHCKGRE TTVTLGVHDV SKTESTQQKI KVEKQIVHPN
110 120 130 140 150
YNFYSNLHDI MLLKLQKKAK VTPAVDVIPL PQPSDFLKPG KMCRAAGWGQ
160 170 180 190 200
TGVTKPTSNT LREVKQRIMD KEACKNYFHY NYNFQVCVGS PRKIRSAYKG
210 220 230 240 250
DSGGPLVCAG VAHGIVSYGR GDAKPPAVFT RISPYVPWIN KVIKGKDLTS
260
LSLHESESPS
Length:260
Mass (Da):28,617
Last modified:November 1, 1997 - v3
Checksum:i076278089C1F8A96
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
O70500O70500_RAT
Mast cell protease 1
Mcpt1 Mcpt2, VCH, rCG_23350
247Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56 – 58TRQ → NRN AA sequence (PubMed:3122823).Curated3
Sequence conflicti70 – 71ET → NE AA sequence (PubMed:3122823).Curated2
Sequence conflicti91 – 94KVEK → ALEI AA sequence (PubMed:8336143).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67915 mRNA Translation: AAB48268.1
S69206 mRNA Translation: AAB30269.1
PIRiJC2125
RefSeqiNP_001264597.1, NM_001277668.1
UniGeneiRn.145977
Rn.202971

Genome annotation databases

EnsembliENSRNOT00000090206; ENSRNOP00000069831; ENSRNOG00000053494
GeneIDi100360872
KEGGirno:100360872
UCSCiRGD:3062 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67915 mRNA Translation: AAB48268.1
S69206 mRNA Translation: AAB30269.1
PIRiJC2125
RefSeqiNP_001264597.1, NM_001277668.1
UniGeneiRn.145977
Rn.202971

3D structure databases

ProteinModelPortaliP09650
SMRiP09650
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028012

Protein family/group databases

MEROPSiS01.149

Proteomic databases

PaxDbiP09650
PRIDEiP09650

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000090206; ENSRNOP00000069831; ENSRNOG00000053494
GeneIDi100360872
KEGGirno:100360872
UCSCiRGD:3062 rat

Organism-specific databases

CTDi100360872
RGDi3062 Mcpt1

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118895
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP09650
KOiK01329
OMAiNLQICVG
OrthoDBiEOG091G0G5F
PhylomeDBiP09650
TreeFamiTF333630

Miscellaneous databases

PROiPR:P09650

Gene expression databases

BgeeiENSRNOG00000053494 Expressed in 9 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP09650 RN

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCPT1_RAT
AccessioniPrimary (citable) accession number: P09650
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: September 12, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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