UniProtKB - P09620 (KEX1_YEAST)
Pheromone-processing carboxypeptidase KEX1
KEX1
Functioni
Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from the precursors of K1, K2 and K28 killer toxins and a-factor (mating pheromone). Involved in the programmed cell death caused by defective N-glycosylation and contributes also to the active cell death program induced by acetic acid stress or during chronological aging. Promotes cell fusion by proteolytically processing substrates that act in parallel to PRM1 as an alternative fusion machine, as cell wall components, or both.
11 PublicationsMiscellaneous
Catalytic activityi
- Preferential release of a C-terminal arginine or lysine residue. EC:3.4.16.6
Kineticsi
- KM=284 µM for benzoyl-Phe-Ala-Arg1 Publication
- KM=516 µM for furylacryloyl-Ala-Arg1 Publication
- KM=962 µM for furylacryloyl-Ala-Lys1 Publication
- Vmax=64.25 µmol/min/mg enzyme toward benzoyl-Phe-Ala-Arg1 Publication
- Vmax=22.35 µmol/min/mg enzyme toward furylacryloyl-Ala-Arg1 Publication
- Vmax=11.55 µmol/min/mg enzyme toward furylacryloyl-Ala-Lys1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 198 | 1 | ||
Active sitei | 405 | By similarity | 1 | |
Active sitei | 470 | By similarity | 1 |
GO - Molecular functioni
- serine-type carboxypeptidase activity Source: SGD
GO - Biological processi
- apoptotic process Source: SGD
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Apoptosis |
Enzyme and pathway databases
BRENDAi | 3.4.16.6, 984 |
Protein family/group databases
ESTHERi | yeast-kex01, Carboxypeptidase_S10 |
MEROPSi | S10.007 |
Names & Taxonomyi
Protein namesi | Recommended name: Pheromone-processing carboxypeptidase KEX1 (EC:3.4.16.6)Alternative name(s): Carboxypeptidase D Killer expression defective protein 1 |
Gene namesi | Name:KEX1 Ordered Locus Names:YGL203C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003171, KEX1 |
VEuPathDBi | FungiDB:YGL203C |
Subcellular locationi
Golgi apparatus
- trans-Golgi network membrane 2 Publications; Single-pass type I membrane protein 2 Publications
Golgi apparatus
- trans-Golgi network Source: SGD
Vacuole
- fungal-type vacuole Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 23 – 616 | LumenalSequence analysisAdd BLAST | 594 | |
Transmembranei | 617 – 637 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 638 – 729 | CytoplasmicSequence analysisAdd BLAST | 92 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 198 | S → A: Inactivates enzyme. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Add BLAST | 22 | |
ChainiPRO_0000004287 | 23 – 729 | Pheromone-processing carboxypeptidase KEX1Add BLAST | 707 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 81 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 459 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 467 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 660 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P09620 |
PaxDbi | P09620 |
PRIDEi | P09620 |
PTM databases
iPTMneti | P09620 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 33053, 223 interactors |
IntActi | P09620, 58 interactors |
MINTi | P09620 |
STRINGi | 4932.YGL203C |
Miscellaneous databases
RNActi | P09620, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P09620 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09620 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 502 – 603 | DisorderedSequence analysisAdd BLAST | 102 | |
Regioni | 674 – 729 | DisorderedSequence analysisAdd BLAST | 56 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 548 – 580 | Acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 581 – 599 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1282, Eukaryota |
HOGENOMi | CLU_008523_11_2_1 |
InParanoidi | P09620 |
OMAi | LFYNSSH |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR001563, Peptidase_S10 IPR033124, Ser_caboxypep_his_AS IPR018202, Ser_caboxypep_ser_AS |
PANTHERi | PTHR11802, PTHR11802, 1 hit |
Pfami | View protein in Pfam PF00450, Peptidase_S10, 1 hit |
PRINTSi | PR00724, CRBOXYPTASEC |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00560, CARBOXYPEPT_SER_HIS, 1 hit PS00131, CARBOXYPEPT_SER_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MFYNRWLGTW LAMSALIRIS VSLPSSEEYK VAYELLPGLS EVPDPSNIPQ
60 70 80 90 100
MHAGHIPLRS EDADEQDSSD LEYFFWKFTN NDSNGNVDRP LIIWLNGGPG
110 120 130 140 150
CSSMDGALVE SGPFRVNSDG KLYLNEGSWI SKGDLLFIDQ PTGTGFSVEQ
160 170 180 190 200
NKDEGKIDKN KFDEDLEDVT KHFMDFLENY FKIFPEDLTR KIILSGESYA
210 220 230 240 250
GQYIPFFANA ILNHNKFSKI DGDTYDLKAL LIGNGWIDPN TQSLSYLPFA
260 270 280 290 300
MEKKLIDESN PNFKHLTNAH ENCQNLINSA STDEAAHFSY QECENILNLL
310 320 330 340 350
LSYTRESSQK GTADCLNMYN FNLKDSYPSC GMNWPKDISF VSKFFSTPGV
360 370 380 390 400
IDSLHLDSDK IDHWKECTNS VGTKLSNPIS KPSIHLLPGL LESGIEIVLF
410 420 430 440 450
NGDKDLICNN KGVLDTIDNL KWGGIKGFSD DAVSFDWIHK SKSTDDSEEF
460 470 480 490 500
SGYVKYDRNL TFVSVYNASH MVPFDKSLVS RGIVDIYSND VMIIDNNGKN
510 520 530 540 550
VMITTDDDSD QDATTESGDK PKENLEEEEQ EAQNEEGKEK EGNKDKDGDD
560 570 580 590 600
DNDNDDDDED DHNSEGDDDD DDDDDEDDNN EKQSNQGLED SRHKSSEYEQ
610 620 630 640 650
EEEEVEEFAE EISMYKHKAV VVTIVTFLIV VLGVYAYDRR VRRKARHTIL
660 670 680 690 700
VDPNNRQHDS PNKTVSWADD LESGLGAEDD LEQDEQLEGG APISSTSNKA
710 720
GSKLKTKKKK KYTSLPNTEI DESFEMTDF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17231 Genomic DNA Translation: AAA34717.1 Z72725 Genomic DNA Translation: CAA96915.1 BK006941 Genomic DNA Translation: DAA07912.1 |
PIRi | A29651 |
RefSeqi | NP_011312.1, NM_001181068.1 |
Genome annotation databases
EnsemblFungii | YGL203C_mRNA; YGL203C; YGL203C |
GeneIDi | 852670 |
KEGGi | sce:YGL203C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17231 Genomic DNA Translation: AAA34717.1 Z72725 Genomic DNA Translation: CAA96915.1 BK006941 Genomic DNA Translation: DAA07912.1 |
PIRi | A29651 |
RefSeqi | NP_011312.1, NM_001181068.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AC5 | X-ray | 2.40 | A | 23-505 | [»] | |
SMRi | P09620 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33053, 223 interactors |
IntActi | P09620, 58 interactors |
MINTi | P09620 |
STRINGi | 4932.YGL203C |
Protein family/group databases
ESTHERi | yeast-kex01, Carboxypeptidase_S10 |
MEROPSi | S10.007 |
PTM databases
iPTMneti | P09620 |
Proteomic databases
MaxQBi | P09620 |
PaxDbi | P09620 |
PRIDEi | P09620 |
Genome annotation databases
EnsemblFungii | YGL203C_mRNA; YGL203C; YGL203C |
GeneIDi | 852670 |
KEGGi | sce:YGL203C |
Organism-specific databases
SGDi | S000003171, KEX1 |
VEuPathDBi | FungiDB:YGL203C |
Phylogenomic databases
eggNOGi | KOG1282, Eukaryota |
HOGENOMi | CLU_008523_11_2_1 |
InParanoidi | P09620 |
OMAi | LFYNSSH |
Enzyme and pathway databases
BRENDAi | 3.4.16.6, 984 |
Miscellaneous databases
EvolutionaryTracei | P09620 |
PROi | PR:P09620 |
RNActi | P09620, protein |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR001563, Peptidase_S10 IPR033124, Ser_caboxypep_his_AS IPR018202, Ser_caboxypep_ser_AS |
PANTHERi | PTHR11802, PTHR11802, 1 hit |
Pfami | View protein in Pfam PF00450, Peptidase_S10, 1 hit |
PRINTSi | PR00724, CRBOXYPTASEC |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00560, CARBOXYPEPT_SER_HIS, 1 hit PS00131, CARBOXYPEPT_SER_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KEX1_YEAST | |
Accessioni | P09620Primary (citable) accession number: P09620 Secondary accession number(s): D6VTV1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 23, 2022 | |
This is version 191 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families