UniProtKB - P09607 (PME21_SOLLC)
Protein
Pectinesterase 2.1
Gene
PME2.1
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Functioni
Pectinesterase may play a role in cell wall metabolism during fruit growth and development prior to ripening and may be required for preparing cell walls for softening by polygalacturonase during fruit ripening.
Miscellaneous
The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.
Catalytic activityi
- [(1→4)-α-D-galacturonosyl methyl ester](n) + n H2O = [(1→4)-α-D-galacturonosyl](n) + n H+ + n methanolEC:3.1.1.11
: pectin degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Pectinesterase, Pectinesterase (101261415), Pectinesterase, Pectinesterase (101244818), Pectinesterase (101246061), Pectinesterase 2.1 (PME2.1), Pectinesterase, Pectinesterase (PMEU1), Pectinesterase (101248642), Pectinesterase, Pectinesterase 2.2 (PME2.2), Pectinesterase (101258375), Pectinesterase (LePME1), Pectinesterase 3 (PME3), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase 1 (PME1.9), Pectinesterase (101255775), Pectinesterase (101260091), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (101260282), Pectinesterase (101244677), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (101261269), Pectinesterase (101248083), Pectinesterase, Pectinesterase, Pectinesterase (101260787), Pectinesterase, Pectinesterase (101253120), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (101260795), Methionine aminopeptidase, Pectinesterase, Pectinesterase, Pectinesterase (101268391), Pectinesterase (101247796), Pectinesterase/pectinesterase inhibitor U1 (PMEU1), Pectinesterase (101260941), Pectinesterase (101260585), Pectinesterase (101247960), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (101266668), Pectinesterase (101262904), Pectinesterase (101261266), Pectinesterase, Pectinesterase (101268168), Pectinesterase (101244375), Pectinesterase, Pectinesterase, Pectinesterase (101261743), Pectinesterase (101257682), Pectinesterase, Pectinesterase (109119119), Pectinesterase (101245713), Pectinesterase, Pectinesterase, Pectinesterase (101264501), Pectinesterase (101266973), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase
- Pectate lyase (778293), Probable pectate lyase P18 (9612), Pectate lyase, Pectate lyase (101257960), Pectate lyase, Pectate lyase, Pectate lyase (101257697), Pectate lyase (101246909), Probable pectate lyase P59 (LAT59), Probable pectate lyase P56 (LAT56), Pectate lyase, Pectate lyase (101260096), Pectate lyase, Pectate lyase (101250914), Pectate lyase (101264933), Pectate lyase (101263458), Pectate lyase (101249735), Pectate lyase, Pectate lyase (101252082), Pectate lyase (101254639), Pectate lyase, Pectate lyase, Pectate lyase (101260911), Pectate lyase (101253459)
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 312 | SubstrateBy similarity | 1 | |
Binding sitei | 342 | SubstrateBy similarity | 1 | |
Sitei | 364 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 365 | Proton donorPROSITE-ProRule annotation | 1 | |
Active sitei | 386 | NucleophilePROSITE-ProRule annotation | 1 | |
Binding sitei | 454 | SubstrateBy similarity | 1 | |
Binding sitei | 456 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- aspartyl esterase activity Source: UniProtKB-KW
- pectinesterase activity Source: GO_Central
- pectinesterase inhibitor activity Source: GO_Central
GO - Biological processi
- cell wall modification Source: InterPro
- fruit ripening Source: UniProtKB-KW
- pectin catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Aspartyl esterase, Hydrolase |
Biological process | Cell wall biogenesis/degradation, Fruit ripening |
Enzyme and pathway databases
UniPathwayi | UPA00545;UER00823 |
Names & Taxonomyi
Protein namesi | Recommended name: Pectinesterase 2.1 (EC:3.1.1.11)Short name: PE 2.1 Alternative name(s): Pectin methylesterase 2.1 |
Gene namesi | Name:PME2.1 |
Organismi | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
Taxonomic identifieri | 4081 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Solanum subgen. Lycopersicon |
Proteomesi |
|
Subcellular locationi
Cell wall
Cell Wall
- cell wall Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Keywords - Cellular componenti
Cell wall, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – ? | Sequence analysis | ||
PropeptideiPRO_0000023488 | ? – 233 | Sequence analysis | ||
ChainiPRO_0000023489 | 234 – 550 | Pectinesterase 2.1Add BLAST | 317 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 179 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 331 ↔ 358 | By similarity | ||
Disulfide bondi | 399 ↔ 433 | By similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P09607 |
Expressioni
Developmental stagei
In ripening fruit.
Gene expression databases
ExpressionAtlasi | P09607, baseline and differential |
Family & Domainsi
Sequence similaritiesi
In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QUQ5, Eukaryota |
HOGENOMi | CLU_012243_9_2_1 |
InParanoidi | P09607 |
OMAi | RCKIDAY |
OrthoDBi | 674407at2759 |
Family and domain databases
Gene3Di | 1.20.140.40, 1 hit 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit |
SMARTi | View protein in SMART SM00856, PMEI, 1 hit |
SUPFAMi | SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit |
TIGRFAMsi | TIGR01614, PME_inhib, 1 hit |
PROSITEi | View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P09607-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATPQQPLLT KTHKQNSIIS FKILTFVVTL FVALFLVVFL VAPYQFEIKH
60 70 80 90 100
SNLCKTAQDS QLCLSYVSDL ISNEIVTSDS DGLSILKKFL VYSVHQMNNA
110 120 130 140 150
IPVVRKIKNQ INDIREQGAL TDCLELLDLS VDLVCDSIAA IDKRSRSEHA
160 170 180 190 200
NAQSWLSGVL TNHVTCLDEL DSFTKAMING TNLDELISRA KVALAMLASV
210 220 230 240 250
TTPNDEVLRP GLGKMPSWVS SRDRKLMESS GKDIGANAVV AKDGTGKYRT
260 270 280 290 300
LAEAVAAAPD KSKTRYVIYV KRGTYKENVE VSSRKMNLMI IGDGMYATII
310 320 330 340 350
TGSLNVVDGS TTFHSATLAA VGKGFILQDI CIQNTAGPAK HQAVALRVGA
360 370 380 390 400
DKSVINRCRI DAYQDTLYAH SQRQFYRDSY VTGTIDFIFG NAAVVFQKCQ
410 420 430 440 450
LVARKPGKYQ QNMVTAQGRT DPNQATGTSI QFCDIIASPD LKPVVKEFPT
460 470 480 490 500
YLGRPWKKYS RTVVMESYLG GLIDPSGWAE WHGDFALKTL YYGEFMNNGP
510 520 530 540 550
GAGTSKRVKW PGYHVITDPA EAMSFTVAKL IQGGSWLRST DVAYVDGLYD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 377 – 378 | RD → QS in CAA30746 (PubMed:3371355).Curated | 2 | |
Sequence conflicti | 377 – 378 | RD → QS in CAA01257 (PubMed:3371355).Curated | 2 | |
Sequence conflicti | 468 | Y → S in CAA30746 (PubMed:3371355).Curated | 1 | |
Sequence conflicti | 468 | Y → S in CAA01257 (PubMed:3371355).Curated | 1 | |
Sequence conflicti | 515 | V → C in AAB67739 (Ref. 2) Curated | 1 | |
Sequence conflicti | 550 | D → DYSDIKLLFVYVTRHL in CAA30746 (PubMed:3371355).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74639 mRNA Translation: CAA52704.1 U50985 mRNA Translation: AAB67739.1 X07910 mRNA Translation: CAA30746.1 A15983 Unassigned RNA Translation: CAA01257.1 |
PIRi | S46528 |
RefSeqi | NP_001233948.1, NM_001247019.1 |
Genome annotation databases
GeneIDi | 544289 |
KEGGi | sly:544289 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74639 mRNA Translation: CAA52704.1 U50985 mRNA Translation: AAB67739.1 X07910 mRNA Translation: CAA30746.1 A15983 Unassigned RNA Translation: CAA01257.1 |
PIRi | S46528 |
RefSeqi | NP_001233948.1, NM_001247019.1 |
3D structure databases
SMRi | P09607 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4081.Solyc07g064180.2.1 |
Proteomic databases
PaxDbi | P09607 |
Genome annotation databases
GeneIDi | 544289 |
KEGGi | sly:544289 |
Phylogenomic databases
eggNOGi | ENOG502QUQ5, Eukaryota |
HOGENOMi | CLU_012243_9_2_1 |
InParanoidi | P09607 |
OMAi | RCKIDAY |
OrthoDBi | 674407at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00545;UER00823 |
Gene expression databases
ExpressionAtlasi | P09607, baseline and differential |
Family and domain databases
Gene3Di | 1.20.140.40, 1 hit 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit |
SMARTi | View protein in SMART SM00856, PMEI, 1 hit |
SUPFAMi | SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit |
TIGRFAMsi | TIGR01614, PME_inhib, 1 hit |
PROSITEi | View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PME21_SOLLC | |
Accessioni | P09607Primary (citable) accession number: P09607 Secondary accession number(s): Q43144, Q43777 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families