UniProtKB - P09601 (HMOX1_HUMAN)
Protein
Heme oxygenase 1
Gene
HMOX1
Organism
Homo sapiens (Human)
Status
Functioni
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Catalytic activityi
- heme b + 3 O2 + 3 reduced [NADPH—hemoprotein reductase] = biliverdin IXα + CO + Fe2+ + H+ + 3 H2O + 3 oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.18By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 18 | Heme | 1 | |
Metal bindingi | 25 | Iron (heme axial ligand) | 1 | |
Binding sitei | 134 | Heme | 1 | |
Binding sitei | 183 | Heme | 1 |
GO - Molecular functioni
- enzyme binding Source: BHF-UCL
- heme binding Source: BHF-UCL
- heme oxygenase (decyclizing) activity Source: BHF-UCL
- identical protein binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- phospholipase D activity Source: Ensembl
- protein homodimerization activity Source: UniProtKB
- structural molecule activity Source: UniProtKB
GO - Biological processi
- angiogenesis Source: BHF-UCL
- cell death Source: BHF-UCL
- cellular iron ion homeostasis Source: CACAO
- cellular response to arsenic-containing substance Source: Ensembl
- cellular response to cadmium ion Source: Ensembl
- cellular response to cisplatin Source: Ensembl
- cellular response to heat Source: UniProtKB
- cellular response to hypoxia Source: UniProtKB
- cellular response to nutrient Source: Ensembl
- cytokine-mediated signaling pathway Source: Reactome
- endothelial cell proliferation Source: BHF-UCL
- erythrocyte homeostasis Source: BHF-UCL
- excretion Source: BHF-UCL
- heme catabolic process Source: BHF-UCL
- heme oxidation Source: BHF-UCL
- intracellular signal transduction Source: BHF-UCL
- intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
- iron ion homeostasis Source: BHF-UCL
- liver regeneration Source: Ensembl
- low-density lipoprotein particle clearance Source: BHF-UCL
- negative regulation of DNA binding Source: Ensembl
- negative regulation of DNA-binding transcription factor activity Source: Ensembl
- negative regulation of epithelial cell apoptotic process Source: Ensembl
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
- negative regulation of leukocyte migration Source: BHF-UCL
- negative regulation of macroautophagy Source: Ensembl
- negative regulation of mast cell cytokine production Source: Ensembl
- negative regulation of mast cell degranulation Source: Ensembl
- negative regulation of muscle cell apoptotic process Source: Ensembl
- negative regulation of neuron apoptotic process Source: Ensembl
- negative regulation of smooth muscle cell proliferation Source: UniProtKB
- negative regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
- positive regulation of angiogenesis Source: BHF-UCL
- positive regulation of apoptotic process Source: Ensembl
- positive regulation of blood vessel diameter Source: BHF-UCL
- positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: BHF-UCL
- positive regulation of cell migration involved in sprouting angiogenesis Source: BHF-UCL
- positive regulation of chemokine production Source: BHF-UCL
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of macroautophagy Source: Ensembl
- positive regulation of smooth muscle cell proliferation Source: UniProtKB
- regulation of angiogenesis Source: BHF-UCL
- regulation of blood pressure Source: Ensembl
- regulation of DNA-binding transcription factor activity Source: BHF-UCL
- regulation of transcription from RNA polymerase II promoter in response to iron Source: Ensembl
- regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: BHF-UCL
- response to drug Source: Ensembl
- response to estrogen Source: Ensembl
- response to hydrogen peroxide Source: BHF-UCL
- response to nicotine Source: BHF-UCL
- response to oxidative stress Source: BHF-UCL
- small GTPase mediated signal transduction Source: Ensembl
- smooth muscle hyperplasia Source: BHF-UCL
- wound healing involved in inflammatory response Source: BHF-UCL
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Apoptosis |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS02027-MONOMER |
BRENDAi | 1.14.99.3, 2681 |
PathwayCommonsi | P09601 |
Reactomei | R-HSA-189483, Heme degradation R-HSA-6785807, Interleukin-4 and Interleukin-13 signaling R-HSA-917937, Iron uptake and transport R-HSA-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
SIGNORi | P09601 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:HMOX1 Synonyms:HO, HO1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:5013, HMOX1 |
MIMi | 141250, gene |
neXtProti | NX_P09601 |
VEuPathDBi | HostDB:ENSG00000100292.16 |
Subcellular locationi
Endoplasmic reticulum
- Microsome 1 Publication
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Cytosol
- cytosol Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
Extracellular region or secreted
- extracellular space Source: BHF-UCL
Nucleus
Plasma Membrane
- caveola Source: Ensembl
Other locations
- membrane Source: ProtInc
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Involvement in diseasei
Heme oxygenase 1 deficiency (HMOX1D)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly.
Related information in OMIMMutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 140 | D → A: Inactive as a heme oxygenase but active as a peroxidase. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 3162 |
MalaCardsi | HMOX1 |
MIMi | 614034, phenotype |
OpenTargetsi | ENSG00000100292 |
Orphaneti | 562509, Heme oxygenase-1 deficiency |
PharmGKBi | PA29341 |
Miscellaneous databases
Pharosi | P09601, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2823 |
DrugBanki | DB07342, 1-(adamantan-1-yl)-2-(1H-imidazol-1-yl)ethanone DB06914, 1-({2-[2-(4-CHLOROPHENYL)ETHYL]-1,3-DIOXOLAN-2-YL}METHYL)-1H-IMIDAZOLE DB02468, 12-Phenylheme DB03906, 2-Phenylheme DB14001, alpha-Tocopherol succinate DB02073, Biliverdine IX Alpha DB14002, D-alpha-Tocopherol acetate DB01942, Formic acid DB00157, NADH DB09221, Polaprezinc DB04912, Stannsoporfin DB04803, Verdoheme DB00163, Vitamin E |
GuidetoPHARMACOLOGYi | 1441 |
Genetic variation databases
BioMutai | HMOX1 |
DMDMi | 123446 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209687 | 1 – 288 | Heme oxygenase 1Add BLAST | 288 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 229 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P09601 |
jPOSTi | P09601 |
MassIVEi | P09601 |
PaxDbi | P09601 |
PeptideAtlasi | P09601 |
PRIDEi | P09601 |
ProteomicsDBi | 52249 |
PTM databases
iPTMneti | P09601 |
PhosphoSitePlusi | P09601 |
Expressioni
Tissue specificityi
Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).1 Publication
Inductioni
Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA.
Gene expression databases
Bgeei | ENSG00000100292, Expressed in layer of synovial tissue and 191 other tissues |
ExpressionAtlasi | P09601, baseline and differential |
Genevisiblei | P09601, HS |
Organism-specific databases
HPAi | ENSG00000100292, Tissue enhanced (lymphoid) |
Interactioni
Binary interactionsi
Hide detailsP09601
GO - Molecular functioni
- enzyme binding Source: BHF-UCL
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109405, 74 interactors |
IntActi | P09601, 55 interactors |
MINTi | P09601 |
STRINGi | 9606.ENSP00000216117 |
Chemistry databases
BindingDBi | P09601 |
Miscellaneous databases
RNActi | P09601, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09601 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09601 |
Family & Domainsi
Sequence similaritiesi
Belongs to the heme oxygenase family.Curated
Phylogenomic databases
eggNOGi | KOG4480, Eukaryota |
GeneTreei | ENSGT00390000017673 |
HOGENOMi | CLU_057050_0_0_1 |
InParanoidi | P09601 |
OMAi | KKSHTMA |
OrthoDBi | 1424194at2759 |
PhylomeDBi | P09601 |
TreeFami | TF314786 |
Family and domain databases
Gene3Di | 1.20.910.10, 1 hit |
InterProi | View protein in InterPro IPR002051, Haem_Oase IPR016053, Haem_Oase-like IPR016084, Haem_Oase-like_multi-hlx IPR018207, Haem_oxygenase_CS |
PANTHERi | PTHR10720, PTHR10720, 1 hit |
Pfami | View protein in Pfam PF01126, Heme_oxygenase, 1 hit |
PIRSFi | PIRSF000343, Haem_Oase, 1 hit |
PRINTSi | PR00088, HAEMOXYGNASE |
SUPFAMi | SSF48613, SSF48613, 1 hit |
PROSITEi | View protein in PROSITE PS00593, HEME_OXYGENASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P09601-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERPQPDSMP QDLSEALKEA TKEVHTQAEN AEFMRNFQKG QVTRDGFKLV
60 70 80 90 100
MASLYHIYVA LEEEIERNKE SPVFAPVYFP EELHRKAALE QDLAFWYGPR
110 120 130 140 150
WQEVIPYTPA MQRYVKRLHE VGRTEPELLV AHAYTRYLGD LSGGQVLKKI
160 170 180 190 200
AQKALDLPSS GEGLAFFTFP NIASATKFKQ LYRSRMNSLE MTPAVRQRVI
210 220 230 240 250
EEAKTAFLLN IQLFEELQEL LTHDTKDQSP SRAPGLRQRA SNKVQDSAPV
260 270 280
ETPRGKPPLN TRSQAPLLRW VLTLSFLVAT VAVGLYAM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB1AHA8 | B1AHA8_HUMAN | Heme oxygenase (biliverdin-producin... | HMOX1 | 187 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_019165 | 7 | D → H1 PublicationCorresponds to variant dbSNP:rs2071747Ensembl. | 1 | |
Natural variantiVAR_022156 | 106 | P → L. Corresponds to variant dbSNP:rs9282702Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06985 mRNA Translation: CAA30045.1 CR456505 mRNA Translation: CAG30391.1 AY460337 Genomic DNA Translation: AAR23262.1 Z82244 Genomic DNA No translation available. M23041 mRNA Translation: AAA50403.1 X14782 Genomic DNA Translation: CAA32886.1 |
CCDSi | CCDS13914.1 |
PIRi | S00325 |
RefSeqi | NP_002124.1, NM_002133.2 |
Genome annotation databases
Ensembli | ENST00000216117; ENSP00000216117; ENSG00000100292 |
GeneIDi | 3162 |
KEGGi | hsa:3162 |
Similar proteinsi
Cross-referencesi
Web resourcesi
SeattleSNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06985 mRNA Translation: CAA30045.1 CR456505 mRNA Translation: CAG30391.1 AY460337 Genomic DNA Translation: AAR23262.1 Z82244 Genomic DNA No translation available. M23041 mRNA Translation: AAA50403.1 X14782 Genomic DNA Translation: CAA32886.1 |
CCDSi | CCDS13914.1 |
PIRi | S00325 |
RefSeqi | NP_002124.1, NM_002133.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N3U | X-ray | 2.58 | A/B | 1-233 | [»] | |
1N45 | X-ray | 1.50 | A/B | 1-233 | [»] | |
1NI6 | X-ray | 2.10 | A/B/C/D | 1-224 | [»] | |
1OYK | X-ray | 2.59 | A/B | 1-233 | [»] | |
1OYL | X-ray | 1.59 | A/B | 1-233 | [»] | |
1OZE | X-ray | 2.19 | A/B | 1-233 | [»] | |
1OZL | X-ray | 1.58 | A/B | 1-233 | [»] | |
1OZR | X-ray | 1.74 | A/B | 1-233 | [»] | |
1OZW | X-ray | 1.55 | A/B | 1-233 | [»] | |
1S13 | X-ray | 2.29 | A/B | 1-233 | [»] | |
1S8C | X-ray | 2.19 | A/B/C/D | 1-233 | [»] | |
1T5P | X-ray | 2.11 | A/B | 1-233 | [»] | |
1TWN | X-ray | 2.20 | A/B | 1-233 | [»] | |
1TWR | X-ray | 2.10 | A/B | 1-233 | [»] | |
1XJZ | X-ray | 1.88 | A/B | 1-233 | [»] | |
1XK0 | X-ray | 2.18 | A/B | 1-233 | [»] | |
1XK1 | X-ray | 2.08 | A/B | 1-233 | [»] | |
1XK2 | X-ray | 2.20 | A/B | 1-233 | [»] | |
1XK3 | X-ray | 2.08 | A/B | 1-233 | [»] | |
3CZY | X-ray | 1.54 | A/B | 1-233 | [»] | |
3HOK | X-ray | 2.19 | A/B | 1-233 | [»] | |
3K4F | X-ray | 2.17 | A/B | 1-233 | [»] | |
3TGM | X-ray | 2.85 | A/B | 1-233 | [»] | |
4WD4 | X-ray | 2.95 | A/B/C/D | 1-288 | [»] | |
5BTQ | X-ray | 2.08 | A/B | 1-233 | [»] | |
6EHA | X-ray | 2.00 | A/B | 1-288 | [»] | |
SMRi | P09601 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109405, 74 interactors |
IntActi | P09601, 55 interactors |
MINTi | P09601 |
STRINGi | 9606.ENSP00000216117 |
Chemistry databases
BindingDBi | P09601 |
ChEMBLi | CHEMBL2823 |
DrugBanki | DB07342, 1-(adamantan-1-yl)-2-(1H-imidazol-1-yl)ethanone DB06914, 1-({2-[2-(4-CHLOROPHENYL)ETHYL]-1,3-DIOXOLAN-2-YL}METHYL)-1H-IMIDAZOLE DB02468, 12-Phenylheme DB03906, 2-Phenylheme DB14001, alpha-Tocopherol succinate DB02073, Biliverdine IX Alpha DB14002, D-alpha-Tocopherol acetate DB01942, Formic acid DB00157, NADH DB09221, Polaprezinc DB04912, Stannsoporfin DB04803, Verdoheme DB00163, Vitamin E |
GuidetoPHARMACOLOGYi | 1441 |
PTM databases
iPTMneti | P09601 |
PhosphoSitePlusi | P09601 |
Genetic variation databases
BioMutai | HMOX1 |
DMDMi | 123446 |
Proteomic databases
EPDi | P09601 |
jPOSTi | P09601 |
MassIVEi | P09601 |
PaxDbi | P09601 |
PeptideAtlasi | P09601 |
PRIDEi | P09601 |
ProteomicsDBi | 52249 |
Protocols and materials databases
Antibodypediai | 266, 1070 antibodies |
DNASUi | 3162 |
Genome annotation databases
Ensembli | ENST00000216117; ENSP00000216117; ENSG00000100292 |
GeneIDi | 3162 |
KEGGi | hsa:3162 |
Organism-specific databases
CTDi | 3162 |
DisGeNETi | 3162 |
GeneCardsi | HMOX1 |
HGNCi | HGNC:5013, HMOX1 |
HPAi | ENSG00000100292, Tissue enhanced (lymphoid) |
MalaCardsi | HMOX1 |
MIMi | 141250, gene 614034, phenotype |
neXtProti | NX_P09601 |
OpenTargetsi | ENSG00000100292 |
Orphaneti | 562509, Heme oxygenase-1 deficiency |
PharmGKBi | PA29341 |
VEuPathDBi | HostDB:ENSG00000100292.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4480, Eukaryota |
GeneTreei | ENSGT00390000017673 |
HOGENOMi | CLU_057050_0_0_1 |
InParanoidi | P09601 |
OMAi | KKSHTMA |
OrthoDBi | 1424194at2759 |
PhylomeDBi | P09601 |
TreeFami | TF314786 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS02027-MONOMER |
BRENDAi | 1.14.99.3, 2681 |
PathwayCommonsi | P09601 |
Reactomei | R-HSA-189483, Heme degradation R-HSA-6785807, Interleukin-4 and Interleukin-13 signaling R-HSA-917937, Iron uptake and transport R-HSA-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
SIGNORi | P09601 |
Miscellaneous databases
BioGRID-ORCSi | 3162, 8 hits in 885 CRISPR screens |
ChiTaRSi | HMOX1, human |
EvolutionaryTracei | P09601 |
GeneWikii | HMOX1 |
GenomeRNAii | 3162 |
Pharosi | P09601, Tchem |
PROi | PR:P09601 |
RNActi | P09601, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100292, Expressed in layer of synovial tissue and 191 other tissues |
ExpressionAtlasi | P09601, baseline and differential |
Genevisiblei | P09601, HS |
Family and domain databases
Gene3Di | 1.20.910.10, 1 hit |
InterProi | View protein in InterPro IPR002051, Haem_Oase IPR016053, Haem_Oase-like IPR016084, Haem_Oase-like_multi-hlx IPR018207, Haem_oxygenase_CS |
PANTHERi | PTHR10720, PTHR10720, 1 hit |
Pfami | View protein in Pfam PF01126, Heme_oxygenase, 1 hit |
PIRSFi | PIRSF000343, Haem_Oase, 1 hit |
PRINTSi | PR00088, HAEMOXYGNASE |
SUPFAMi | SSF48613, SSF48613, 1 hit |
PROSITEi | View protein in PROSITE PS00593, HEME_OXYGENASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HMOX1_HUMAN | |
Accessioni | P09601Primary (citable) accession number: P09601 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 210 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families