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Protein

Bifunctional protein PutA

Gene

putA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.

Catalytic activityi

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol.
L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH.

Cofactori

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate from L-proline.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Bifunctional protein PutA (putA)
  2. Bifunctional protein PutA (putA)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei883By similarity1
Active sitei917By similarity1

GO - Molecular functioni

GO - Biological processi

  • proline biosynthetic process Source: InterPro
  • proline catabolic process to glutamate Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Multifunctional enzyme, Oxidoreductase, Repressor
Biological processProline metabolism, Transcription, Transcription regulation
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:PUTA-MONOMER
MetaCyc:PUTA-MONOMER
BRENDAi1.2.1.88 2026
1.5.5.2 2026
UniPathwayi
UPA00261;UER00373

UPA00261;UER00374

Protein family/group databases

MoonProtiP09546

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein PutA
Including the following 2 domains:
Proline dehydrogenase (EC:1.5.5.2)
Alternative name(s):
Proline oxidase
Delta-1-pyrroline-5-carboxylate dehydrogenase (EC:1.2.1.88)
Short name:
P5C dehydrogenase
Alternative name(s):
L-glutamate gamma-semialdehyde dehydrogenase
Gene namesi
Name:putA
Synonyms:poaA
Ordered Locus Names:b1014, JW0999
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10801 putA

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

DrugBankiDB03147 Flavin adenine dinucleotide
DB04398 Lactic Acid
DB03051 Tetrahydrofuran-2-Carboxylic Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000565241 – 1320Bifunctional protein PutAAdd BLAST1320

Proteomic databases

EPDiP09546
PaxDbiP09546
PRIDEiP09546

Expressioni

Inductioni

By proline, autorepression and catabolite repression, and is potentially nitrogen controlled.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-369718,EBI-369718

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4262846, 27 interactors
DIPiDIP-10620N
IntActiP09546, 24 interactors
MINTiP09546
STRINGi316385.ECDH10B_1086

Structurei

Secondary structure

11320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP09546
SMRiP09546
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09546

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni228 – 574Proline dehydrogenaseAdd BLAST347
Regioni653 – 1119Aldehyde dehydrogenaseAdd BLAST467

Sequence similaritiesi

In the N-terminal section; belongs to the proline dehydrogenase family.Curated
In the C-terminal section; belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105C26 Bacteria
COG0506 LUCA
COG4230 LUCA
HOGENOMiHOG000253911
InParanoidiP09546
KOiK13821
OMAiVRIYAPV
PhylomeDBiP09546

Family and domain databases

Gene3Di1.10.1220.10, 1 hit
1.20.5.550, 1 hit
3.40.309.10, 1 hit
3.40.605.10, 2 hits
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
IPR013321 Arc_rbn_hlx_hlx
IPR025703 Bifunct_PutA
IPR005933 Delta1-pyrroline-5-COlate_DH-3
IPR029041 FAD-linked_oxidoreductase-like
IPR024090 PRODH_PutA_dom_I
IPR024089 PRODH_PutA_dom_I/II
IPR024082 PRODH_PutA_dom_II
IPR002872 Proline_DH_dom
IPR010985 Ribbon_hlx_hlx
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
PF01619 Pro_dh, 1 hit
PF14850 Pro_dh-DNA_bdg, 1 hit
PIRSFiPIRSF000197 Bifunct_PutA, 1 hit
SUPFAMiSSF47598 SSF47598, 1 hit
SSF51730 SSF51730, 1 hit
SSF53720 SSF53720, 1 hit
SSF81935 SSF81935, 1 hit
TIGRFAMsiTIGR01238 D1pyr5carbox3, 1 hit
PROSITEiView protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

Sequencei

Sequence statusi: Complete.

P09546-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGTTTMGVKL DDATRERIKS AATRIDRTPH WLIKQAIFSY LEQLENSDTL
60 70 80 90 100
PELPALLSGA ANESDEAPTP AEEPHQPFLD FAEQILPQSV SRAAITAAYR
110 120 130 140 150
RPETEAVSML LEQARLPQPV AEQAHKLAYQ LADKLRNQKN ASGRAGMVQG
160 170 180 190 200
LLQEFSLSSQ EGVALMCLAE ALLRIPDKAT RDALIRDKIS NGNWQSHIGR
210 220 230 240 250
SPSLFVNAAT WGLLFTGKLV STHNEASLSR SLNRIIGKSG EPLIRKGVDM
260 270 280 290 300
AMRLMGEQFV TGETIAEALA NARKLEEKGF RYSYDMLGEA ALTAADAQAY
310 320 330 340 350
MVSYQQAIHA IGKASNGRGI YEGPGISIKL SALHPRYSRA QYDRVMEELY
360 370 380 390 400
PRLKSLTLLA RQYDIGINID AEESDRLEIS LDLLEKLCFE PELAGWNGIG
410 420 430 440 450
FVIQAYQKRC PLVIDYLIDL ATRSRRRLMI RLVKGAYWDS EIKRAQMDGL
460 470 480 490 500
EGYPVYTRKV YTDVSYLACA KKLLAVPNLI YPQFATHNAH TLAAIYQLAG
510 520 530 540 550
QNYYPGQYEF QCLHGMGEPL YEQVTGKVAD GKLNRPCRIY APVGTHETLL
560 570 580 590 600
AYLVRRLLEN GANTSFVNRI ADTSLPLDEL VADPVTAVEK LAQQEGQTGL
610 620 630 640 650
PHPKIPLPRD LYGHGRDNSA GLDLANEHRL ASLSSALLNS ALQKWQALPM
660 670 680 690 700
LEQPVAAGEM SPVINPAEPK DIVGYVREAT PREVEQALES AVNNAPIWFA
710 720 730 740 750
TPPAERAAIL HRAAVLMESQ MQQLIGILVR EAGKTFSNAI AEVREAVDFL
760 770 780 790 800
HYYAGQVRDD FANETHRPLG PVVCISPWNF PLAIFTGQIA AALAAGNSVL
810 820 830 840 850
AKPAEQTPLI AAQGIAILLE AGVPPGVVQL LPGRGETVGA QLTGDDRVRG
860 870 880 890 900
VMFTGSTEVA TLLQRNIASR LDAQGRPIPL IAETGGMNAM IVDSSALTEQ
910 920 930 940 950
VVVDVLASAF DSAGQRCSAL RVLCLQDEIA DHTLKMLRGA MAECRMGNPG
960 970 980 990 1000
RLTTDIGPVI DSEAKANIER HIQTMRSKGR PVFQAVRENS EDAREWQSGT
1010 1020 1030 1040 1050
FVAPTLIELD DFAELQKEVF GPVLHVVRYN RNQLPELIEQ INASGYGLTL
1060 1070 1080 1090 1100
GVHTRIDETI AQVTGSAHVG NLYVNRNMVG AVVGVQPFGG EGLSGTGPKA
1110 1120 1130 1140 1150
GGPLYLYRLL ANRPESALAV TLARQDAKYP VDAQLKAALT QPLNALREWA
1160 1170 1180 1190 1200
ANRPELQALC TQYGELAQAG TQRLLPGPTG ERNTWTLLPR ERVLCIADDE
1210 1220 1230 1240 1250
QDALTQLAAV LAVGSQVLWP DDALHRQLVK ALPSAVSERI QLAKAENITA
1260 1270 1280 1290 1300
QPFDAVIFHG DSDQLRALCE AVAARDGTIV SVQGFARGES NILLERLYIE
1310 1320
RSLSVNTAAA GGNASLMTIG
Length:1,320
Mass (Da):143,815
Last modified:November 1, 1997 - v3
Checksum:iE4920143D536F7F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti531G → A in AAB59985 (PubMed:7966312).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05212 Genomic DNA Translation: AAB59985.1
U00096 Genomic DNA Translation: AAC74099.1
AP009048 Genomic DNA Translation: BAA35791.1
X05653 Genomic DNA Translation: CAA29141.1 Sequence problems.
PIRiD64843
RefSeqiNP_415534.1, NC_000913.3
WP_001326840.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74099; AAC74099; b1014
BAA35791; BAA35791; BAA35791
GeneIDi945600
KEGGiecj:JW0999
eco:b1014
PATRICifig|1411691.4.peg.1257

Similar proteinsi

Entry informationi

Entry nameiPUTA_ECOLI
AccessioniPrimary (citable) accession number: P09546
Secondary accession number(s): P78296
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 183 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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