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Protein

Ras-related protein Rab-7a

Gene

Rab7a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades (By similarity). Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism (By similarity). Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes (By similarity). Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria (By similarity). Plays a role in the fusion of phagosomes with lysosomes (By similarity). Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses (By similarity). Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium) (By similarity). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts (PubMed:16040606). Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:16306406). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation (By similarity). Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTP8
Nucleotide bindingi34 – 40GTP7
Nucleotide bindingi63 – 67GTP5
Nucleotide bindingi125 – 128GTP4
Nucleotide bindingi156 – 157GTP2

GO - Molecular functioni

  • GDP binding Source: RGD
  • GTPase activity Source: GO_Central
  • GTP binding Source: RGD
  • Rac GTPase binding Source: RGD

GO - Biological processi

Keywordsi

Biological processAutophagy, Lipid degradation, Lipid metabolism, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2132295 MHC class II antigen presentation
R-RNO-6798695 Neutrophil degranulation
R-RNO-8854214 TBC/RABGAPs
R-RNO-8873719 RAB geranylgeranylation
R-RNO-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-7a
Alternative name(s):
Ras-related protein BRL-RAS
Ras-related protein p23
Gene namesi
Name:Rab7a
Synonyms:Rab7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi61908 Rab7a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lipid droplet, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001211242 – 207Ras-related protein Rab-7aAdd BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei72PhosphoserineCombined sources1
Lipidationi205S-geranylgeranyl cysteineBy similarity1
Modified residuei207Cysteine methyl esterBy similarity1
Lipidationi207S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP09527
PRIDEiP09527

PTM databases

iPTMnetiP09527
PhosphoSitePlusiP09527
SwissPalmiP09527

Expressioni

Tissue specificityi

Expressed in osteoclasts and in neurons.2 Publications

Gene expression databases

BgeeiENSRNOG00000012247 Expressed in 10 organ(s), highest expression level in lung
ExpressionAtlasiP09527 baseline and differential
GenevisibleiP09527 RN

Interactioni

Subunit structurei

Interacts with NTRK1/TRKA (PubMed:16306406). Interacts with RILP (By similarity). Interacts with PSMA7 (By similarity). Interacts with RNF115 (By similarity). Interacts with and FYCO1 (By similarity). Interacts with the PIK3C3/VPS34-PIK3R4 complex (By similarity). The GTP-bound form interacts with OSBPL1A (By similarity). The GTP-bound form interacts with RAC1 (PubMed:16040606). Interacts with CLN3 (By similarity). Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (PubMed:18756270, PubMed:15186776). Interacts with C9orf72 (By similarity). Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4). Interacts with CLN5 (By similarity). Interacts with PLEKHM1 (via N- and C-terminus) (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ChmP377272EBI-916225,EBI-1039231

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248093, 2 interactors
IntActiP09527, 8 interactors
MINTiP09527
STRINGi10116.ENSRNOP00000016432

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP09527
SMRiP09527
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09527

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0394 Eukaryota
ENOG410XNZV LUCA
GeneTreeiENSGT00760000119125
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP09527
KOiK07897
OMAiLMQQFVN
OrthoDBiEOG091G0JIV
PhylomeDBiP09527
TreeFamiTF105605

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P09527-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV
60 70 80 90 100
MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD
110 120 130 140 150
SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP
160 170 180 190 200
YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNERAKA

SAESCSC
Length:207
Mass (Da):23,504
Last modified:October 1, 1996 - v2
Checksum:iA2AF33B02F672971
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K930A0A0G2K930_RAT
Ras-related protein Rab-7a
Rab7a
188Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12535 mRNA Translation: CAA31053.1
AF286535 mRNA Translation: AAG00543.1
AB158427 mRNA Translation: BAE16999.1
AB158428 mRNA Translation: BAE17000.1
BC072470 mRNA Translation: AAH72470.1
PIRiS01934
RefSeqiNP_076440.1, NM_023950.3
XP_006236911.1, XM_006236849.3
UniGeneiRn.1425

Genome annotation databases

EnsembliENSRNOT00000016432; ENSRNOP00000016432; ENSRNOG00000012247
GeneIDi29448
KEGGirno:29448
UCSCiRGD:61908 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12535 mRNA Translation: CAA31053.1
AF286535 mRNA Translation: AAG00543.1
AB158427 mRNA Translation: BAE16999.1
AB158428 mRNA Translation: BAE17000.1
BC072470 mRNA Translation: AAH72470.1
PIRiS01934
RefSeqiNP_076440.1, NM_023950.3
XP_006236911.1, XM_006236849.3
UniGeneiRn.1425

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VG0X-ray2.20B1-207[»]
1VG1X-ray1.90A1-185[»]
1VG8X-ray1.70A/B/C/D1-207[»]
1VG9X-ray2.50B/D/F/H1-185[»]
ProteinModelPortaliP09527
SMRiP09527
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248093, 2 interactors
IntActiP09527, 8 interactors
MINTiP09527
STRINGi10116.ENSRNOP00000016432

PTM databases

iPTMnetiP09527
PhosphoSitePlusiP09527
SwissPalmiP09527

Proteomic databases

PaxDbiP09527
PRIDEiP09527

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016432; ENSRNOP00000016432; ENSRNOG00000012247
GeneIDi29448
KEGGirno:29448
UCSCiRGD:61908 rat

Organism-specific databases

CTDi7879
RGDi61908 Rab7a

Phylogenomic databases

eggNOGiKOG0394 Eukaryota
ENOG410XNZV LUCA
GeneTreeiENSGT00760000119125
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP09527
KOiK07897
OMAiLMQQFVN
OrthoDBiEOG091G0JIV
PhylomeDBiP09527
TreeFamiTF105605

Enzyme and pathway databases

ReactomeiR-RNO-2132295 MHC class II antigen presentation
R-RNO-6798695 Neutrophil degranulation
R-RNO-8854214 TBC/RABGAPs
R-RNO-8873719 RAB geranylgeranylation
R-RNO-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

EvolutionaryTraceiP09527
PROiPR:P09527

Gene expression databases

BgeeiENSRNOG00000012247 Expressed in 10 organ(s), highest expression level in lung
ExpressionAtlasiP09527 baseline and differential
GenevisibleiP09527 RN

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB7A_RAT
AccessioniPrimary (citable) accession number: P09527
Secondary accession number(s): Q4AEF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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