UniProtKB - P09507 (RDRP_TYYVF)
Protein
Protein P1-P2
Gene
ORF1/ORF2
Organism
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1)
Status
Functioni
Precursor from which the RNA-dependent RNA polymerase (RdRp) is probably released. RNA-dependent RNA polymerase plays an essential role in virus replication (Potential).Curated
Catalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 254 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 289 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 357 | For protease activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- nucleotide binding Source: UniProtKB-KW
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- serine-type endopeptidase activity Source: InterPro
GO - Biological processi
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Serine protease, Transferase |
Biological process | Viral RNA replication |
Ligand | Nucleotide-binding |
Names & Taxonomyi
Subcellular locationi
- Membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 115 – 135 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 137 – 157 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 167 – 187 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000222398 | 23 – 1035 | Protein P1-P2Add BLAST | 1013 | |
ChainiPRO_0000390903 | 204 – 400 | Serine proteaseSequence analysisAdd BLAST | 197 | |
ChainiPRO_0000390904 | 401 – 1035 | RNA-directed RNA polymeraseSequence analysisAdd BLAST | 635 |
Post-translational modificationi
Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the RdRp (Potential). Cleavages have been shown in the P1 protein, but since the N-terminus containing the serine protease is shared between P1 and P1-P2, cleavages should also occur within the P1-P2 protein.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 203 – 204 | Cleavage; by viral serine proteaseSequence analysis | 2 | |
Sitei | 400 – 401 | Cleavage; by viral serine proteaseBy similarity | 2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 206 – 400 | Peptidase S39PROSITE-ProRule annotationAdd BLAST | 195 | |
Domaini | 829 – 944 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 116 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 818 – 821 | Poly-Thr | 4 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR000382, Peptidase_S39B_luteovirus IPR001795, RNA-dir_pol_luteovirus IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF02122, Peptidase_S39, 1 hit PF02123, RdRP_4, 1 hit |
PRINTSi | PR00914, LVIRUSRNAPOL |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform RNA-directed RNA polymerase (identifier: P09507-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYSKLMFFFA LCSISFLFTS EAASTMLLES SYLPLNQSYA PGFLYKRDML
60 70 80 90 100
PPPLQAVLTY TCPEPRPLAE ESYNDLLRAI SQKSSSDFQN AYSLALSFSS
110 120 130 140 150
DFYQHGLKTL KDVSFLAVEK FLWGLTRLWS SLILASFSAL WWLVSNFTTP
160 170 180 190 200
VFCLALLYTV TKYMVKTVSF LFGGLPIWII SIAFSLLKKS FSALRSTPKC
210 220 230 240 250
LYEKAIDGFK SFTIPQSPPK SCVIPITHAS GNHAGYASCI KLYNGENALM
260 270 280 290 300
TATHVLRDCP NAVAVSAKGL KTRIPLAEFK TIAKSDKGDV TLLRGPPNWE
310 320 330 340 350
GLLGCKAANV ITAANLAKCK ASIYSFDRDG WVSSYAEIVG SEGTDVMVLS
360 370 380 390 400
HTEGGHSGSP YFNGKTILGV HSGASATGNY NLMAPIPSLP GLTSPTYVFE
410 420 430 440 450
TTAPQGRVFA QEDIAEIEGL YAQVMKRVQQ AEDFKPKTGK YWGDMEDDED
460 470 480 490 500
IFFESKEDLS GKRSARHRPR NKRRRQLHPK DKQRRWERDD GENNLISSGK
510 520 530 540 550
DKSREHREES DRGDLRESDE NSEIPPQKSP KETAGEFERY FSSLYNWEVP
560 570 580 590 600
TSPREVPGFR HCGKLPQYYH PKQKEESSWG KTLVGNHPAL GEKTSGFGWP
610 620 630 640 650
KFGPEAELKS LRLQASRWLE RAQSAEIPSD AERERVIQKT ADVYHPCQTN
660 670 680 690 700
GPAATRGGTL TWNNFMIDFK QAVFSLEFDA GIELPYIAYG KPTHRGWVED
710 720 730 740 750
QKLLPILAQL TFFRLQKMLE VNFEDMGPEE LVRNGLCDPI RLFVKGEPHK
760 770 780 790 800
QAKLDEGRYR LIMSVSLVDQ LVARVLFQNQ NKREIALWRA IPSKPGFGLS
810 820 830 840 850
TDEQVLDFVE SLARQVGTTT TEVVANWKNY LTPTDCSGFD WSVADWMLHD
860 870 880 890 900
DMIVRNRLTI DLNPATERLR SCWLRCISNS VLCLSDGTLL AQIHPGVQKS
910 920 930 940 950
GSYNTSSSNS RIRVMAAFHT GAIWAMAMGD DALESNPADL AAYKKLGFKV
960 970 980 990 1000
EVSGQLEFCS HIFRAPDLAL PVNENKMIYK LIYGYNPGSG NAEVVSNYLA
1010 1020 1030
ACFSVLNELR HDPASVELLY SWLVDPVLPQ KIPGE
Note: Produced by -1 ribosomal frameshifting between codons 461 and 462.
Isoform Protein P1 (identifier: P09506-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P09506.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13063 Genomic RNA Translation: CAA31464.2 |
PIRi | S01940, RRVQFL |
RefSeqi | NP_620485.2, NC_003743.1 |
Genome annotation databases
GeneIDi | 940481 |
KEGGi | vg:940481 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13063 Genomic RNA Translation: CAA31464.2 |
PIRi | S01940, RRVQFL |
RefSeqi | NP_620485.2, NC_003743.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
GeneIDi | 940481 |
KEGGi | vg:940481 |
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR000382, Peptidase_S39B_luteovirus IPR001795, RNA-dir_pol_luteovirus IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF02122, Peptidase_S39, 1 hit PF02123, RdRP_4, 1 hit |
PRINTSi | PR00914, LVIRUSRNAPOL |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RDRP_TYYVF | |
Accessioni | P09507Primary (citable) accession number: P09507 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | June 20, 2002 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |