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Protein

Glutathione S-transferase Mu 1

Gene

GSTM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Glutathione1 Publication1 Publication1
Binding sitei116Substrate1

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • glutathione binding Source: BHF-UCL
  • glutathione transferase activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation
SABIO-RKiP09488
SIGNORiP09488

Chemistry databases

SwissLipidsiSLP:000001613

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 1 (EC:2.5.1.18)
Alternative name(s):
GST HB subunit 4
GST class-mu 1
GSTM1-1
GSTM1a-1a
GSTM1b-1b
GTH4
Gene namesi
Name:GSTM1
Synonyms:GST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000134184.12
HGNCiHGNC:4632 GSTM1
MIMi138350 gene
neXtProtiNX_P09488

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7Y → F: Reduces catalytic activity 100-fold. 1 Publication1
Mutagenesisi108H → Q: Reduces catalytic activity by half. 2 Publications1
Mutagenesisi108H → S: Changes the properties of the enzyme toward some substrates. 2 Publications1
Mutagenesisi109M → I: Reduces catalytic activity by half. 1 Publication1
Mutagenesisi116Y → A: Reduces catalytic activity 10-fold. 1 Publication1
Mutagenesisi116Y → F: Slight increase of catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi2944
OpenTargetsiENSG00000134184
PharmGKBiPA182

Chemistry databases

ChEMBLiCHEMBL2081
DrugBankiDB00993 Azathioprine
DB01008 Busulfan
DB00958 Carboplatin
DB00515 Cisplatin
DB03314 Fluorotryptophane
DB00143 Glutathione
DB00526 Oxaliplatin
DB02165 Zinc Trihydroxide

Polymorphism and mutation databases

BioMutaiGSTM1
DMDMi121735

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved6 Publications
ChainiPRO_00001858162 – 218Glutathione S-transferase Mu 1Add BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphothreonineBy similarity1
Modified residuei210PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP09488
PaxDbiP09488
PeptideAtlasiP09488
PRIDEiP09488
ProteomicsDBi52227
52228 [P09488-2]

PTM databases

iPTMnetiP09488
PhosphoSitePlusiP09488

Expressioni

Tissue specificityi

Liver (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000134184 Expressed in 92 organ(s), highest expression level in liver
CleanExiHS_GSTM1
ExpressionAtlasiP09488 baseline and differential
GenevisibleiP09488 HS

Organism-specific databases

HPAiCAB022669
CAB047357
HPA055972
HPA055973

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109199, 8 interactors
IntActiP09488, 6 interactors
STRINGi9606.ENSP00000311469

Chemistry databases

BindingDBiP09488

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP09488
SMRiP09488
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09488

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 88GST N-terminalAdd BLAST87
Domaini90 – 208GST C-terminalAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 8Glutathione binding1 Publication1 Publication2
Regioni43 – 46Glutathione binding1 Publication1 Publication4
Regioni59 – 60Glutathione binding1 Publication1 Publication2
Regioni72 – 73Glutathione binding1 Publication1 Publication2

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated

Phylogenomic databases

eggNOGiENOG410IN5J Eukaryota
ENOG4110YU0 LUCA
GeneTreeiENSGT00550000074559
HOGENOMiHOG000115735
HOVERGENiHBG106842
InParanoidiP09488
KOiK00799
PhylomeDBiP09488
TreeFamiTF353040

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR003081 GST_mu
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01267 GSTRNSFRASEM
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P09488-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPMILGYWDI RGLAHAIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK
60 70 80 90 100
FKLGLDFPNL PYLIDGAHKI TQSNAILCYI ARKHNLCGET EEEKIRVDIL
110 120 130 140 150
ENQTMDNHMQ LGMICYNPEF EKLKPKYLEE LPEKLKLYSE FLGKRPWFAG
160 170 180 190 200
NKITFVDFLV YDVLDLHRIF EPKCLDAFPN LKDFISRFEG LEKISAYMKS
210
SRFLPRPVFS KMAVWGNK
Length:218
Mass (Da):25,712
Last modified:January 23, 2007 - v3
Checksum:i98FB03E87B83A31B
GO
Isoform 2 (identifier: P09488-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-189: Missing.

Show »
Length:181
Mass (Da):21,253
Checksum:i1FB16A0418115A26
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EWW9E7EWW9_HUMAN
Glutathione S-transferase Mu 1
GSTM1
237Annotation score:
B9ZVX7B9ZVX7_HUMAN
Glutathione S-transferase Mu 1
GSTM1
149Annotation score:
H3BRM6H3BRM6_HUMAN
Glutathione S-transferase Mu 1
GSTM1
102Annotation score:
H3BQT3H3BQT3_HUMAN
Glutathione S-transferase Mu 1
GSTM1
85Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44S → T in CAA48636 (PubMed:8471052).Curated1
Sequence conflicti207P → T in AAT06767 (Ref. 4) Curated1

Polymorphismi

There are two alleles; GSTM1A and GSTM1B which differ in position 173. The sequence shown is that of allele GSTM1A.4 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_003617173K → N in allele GSTM1B. 4 PublicationsCorresponds to variant dbSNP:rs1065411Ensembl.1
Natural variantiVAR_014497210S → T. Corresponds to variant dbSNP:rs449856Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036618153 – 189Missing in isoform 2. 2 PublicationsAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08020 mRNA Translation: CAA30821.1
J03817 mRNA Translation: AAA59203.1
AY510272 mRNA Translation: AAR85979.1
AY532926 mRNA Translation: AAT06767.1
AY532927 mRNA Translation: AAT06768.1
CR541868 mRNA Translation: CAG46666.1
BC024005 mRNA Translation: AAH24005.1
X68676 Genomic DNA Translation: CAA48636.1
X51451 Genomic DNA Translation: CAA35817.1
CCDSiCCDS809.1 [P09488-1]
CCDS810.1 [P09488-2]
PIRiS01719
RefSeqiNP_000552.2, NM_000561.3 [P09488-1]
NP_666533.1, NM_146421.2 [P09488-2]
UniGeneiHs.301961

Genome annotation databases

EnsembliENST00000309851; ENSP00000311469; ENSG00000134184 [P09488-1]
ENST00000349334; ENSP00000234981; ENSG00000134184 [P09488-2]
GeneIDi2944
KEGGihsa:2944

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

SHMPD

The Singapore human mutation and polymorphism database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08020 mRNA Translation: CAA30821.1
J03817 mRNA Translation: AAA59203.1
AY510272 mRNA Translation: AAR85979.1
AY532926 mRNA Translation: AAT06767.1
AY532927 mRNA Translation: AAT06768.1
CR541868 mRNA Translation: CAG46666.1
BC024005 mRNA Translation: AAH24005.1
X68676 Genomic DNA Translation: CAA48636.1
X51451 Genomic DNA Translation: CAA35817.1
CCDSiCCDS809.1 [P09488-1]
CCDS810.1 [P09488-2]
PIRiS01719
RefSeqiNP_000552.2, NM_000561.3 [P09488-1]
NP_666533.1, NM_146421.2 [P09488-2]
UniGeneiHs.301961

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GTUX-ray2.68A/B/C/D2-218[»]
1XW6X-ray1.90A/B/C/D1-218[»]
1XWKX-ray2.30A/B/C1-218[»]
1YJ6X-ray2.50A/B/C1-218[»]
2F3MX-ray2.70A/B/C/D/E/F1-218[»]
ProteinModelPortaliP09488
SMRiP09488
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109199, 8 interactors
IntActiP09488, 6 interactors
STRINGi9606.ENSP00000311469

Chemistry databases

BindingDBiP09488
ChEMBLiCHEMBL2081
DrugBankiDB00993 Azathioprine
DB01008 Busulfan
DB00958 Carboplatin
DB00515 Cisplatin
DB03314 Fluorotryptophane
DB00143 Glutathione
DB00526 Oxaliplatin
DB02165 Zinc Trihydroxide
SwissLipidsiSLP:000001613

PTM databases

iPTMnetiP09488
PhosphoSitePlusiP09488

Polymorphism and mutation databases

BioMutaiGSTM1
DMDMi121735

Proteomic databases

EPDiP09488
PaxDbiP09488
PeptideAtlasiP09488
PRIDEiP09488
ProteomicsDBi52227
52228 [P09488-2]

Protocols and materials databases

DNASUi2944
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309851; ENSP00000311469; ENSG00000134184 [P09488-1]
ENST00000349334; ENSP00000234981; ENSG00000134184 [P09488-2]
GeneIDi2944
KEGGihsa:2944

Organism-specific databases

CTDi2944
DisGeNETi2944
EuPathDBiHostDB:ENSG00000134184.12
GeneCardsiGSTM1
HGNCiHGNC:4632 GSTM1
HPAiCAB022669
CAB047357
HPA055972
HPA055973
MIMi138350 gene
neXtProtiNX_P09488
OpenTargetsiENSG00000134184
PharmGKBiPA182
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IN5J Eukaryota
ENOG4110YU0 LUCA
GeneTreeiENSGT00550000074559
HOGENOMiHOG000115735
HOVERGENiHBG106842
InParanoidiP09488
KOiK00799
PhylomeDBiP09488
TreeFamiTF353040

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation
SABIO-RKiP09488
SIGNORiP09488

Miscellaneous databases

ChiTaRSiGSTM1 human
EvolutionaryTraceiP09488
GeneWikiiGlutathione_S-transferase_Mu_1
GenomeRNAii2944
PROiPR:P09488
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134184 Expressed in 92 organ(s), highest expression level in liver
CleanExiHS_GSTM1
ExpressionAtlasiP09488 baseline and differential
GenevisibleiP09488 HS

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR003081 GST_mu
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01267 GSTRNSFRASEM
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSTM1_HUMAN
AccessioniPrimary (citable) accession number: P09488
Secondary accession number(s): Q5GHG0
, Q6FH88, Q8TC98, Q9UC96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 195 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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