UniProtKB - P09487 (PPBT_BOVIN)
Protein
Alkaline phosphatase, tissue-nonspecific isozyme
Gene
ALPL
Organism
Bos taurus (Bovine)
Status
Functioni
This isozyme plays a key role in skeletal mineralization by regulating levels of diphosphate (PPi).By similarity
Miscellaneous
In most mammals there are four different isozymes: placental, germ cell, intestinal and tissue non-specific (liver/bone/kidney).
Catalytic activityi
- EC:3.1.3.1PROSITE-ProRule annotationBy similarity
Cofactori
Protein has several cofactor binding sites:- Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
- Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 60 | MagnesiumBy similarity | 1 | |
Metal bindingi | 60 | Zinc 1By similarity | 1 | |
Active sitei | 110 | Phosphoserine intermediateBy similarity | 1 | |
Metal bindingi | 110 | Zinc 1By similarity | 1 | |
Metal bindingi | 173 | MagnesiumBy similarity | 1 | |
Metal bindingi | 332 | MagnesiumBy similarity | 1 | |
Metal bindingi | 337 | Zinc 2By similarity | 1 | |
Metal bindingi | 341 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 378 | Zinc 1By similarity | 1 | |
Metal bindingi | 379 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 454 | Zinc 2; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- alkaline phosphatase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- pyrophosphatase activity Source: Ensembl
GO - Biological processi
- biomineral tissue development Source: AgBase
- cellular response to organic cyclic compound Source: Ensembl
- dephosphorylation Source: GO_Central
- developmental process involved in reproduction Source: Ensembl
- endochondral ossification Source: Ensembl
- response to antibiotic Source: Ensembl
- response to vitamin D Source: Ensembl
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.1.3.1, 908 |
Names & Taxonomyi
Protein namesi | Recommended name: Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1By similarity)Short name: AP-TNAP Short name: TNSALP Alternative name(s): Alkaline phosphatase liver/bone/kidney isozyme |
Gene namesi | Name:ALPL |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:25850, ALPL |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Extracellular region or secreted
- extracellular matrix Source: Ensembl
- extracellular membrane-bounded organelle Source: AgBase
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- anchored component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | 1 PublicationAdd BLAST | 17 | |
ChainiPRO_0000024019 | 18 – 499 | Alkaline phosphatase, tissue-nonspecific isozymeAdd BLAST | 482 | |
PropeptideiPRO_0000024020 | 500 – 524 | Removed in mature formSequence analysisAdd BLAST | 25 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 110 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 139 ↔ 201 | By similarity | ||
Glycosylationi | 140 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 230 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 271 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 303 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 430 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 489 ↔ 497 | By similarity | ||
Lipidationi | 499 | GPI-anchor amidated serineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, PhosphoproteinProteomic databases
PaxDbi | P09487 |
PRIDEi | P09487 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000008951, Expressed in adult mammalian kidney and 17 other tissues |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000011783 |
Chemistry databases
BindingDBi | P09487 |
Family & Domainsi
Sequence similaritiesi
Belongs to the alkaline phosphatase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4126, Eukaryota |
GeneTreei | ENSGT00950000183063 |
HOGENOMi | CLU_008539_4_0_1 |
InParanoidi | P09487 |
OMAi | IDMAHHA |
OrthoDBi | 416703at2759 |
TreeFami | TF323513 |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P09487-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MISPFLLLAI GTCFASSLVP EKEKDPKYWR DQAQQTLKNA LRLQTLNTNV
60 70 80 90 100
AKNVIMFLGD GMGVSTVTAA RILKGQLHHS PGEETKLEMD KFPYVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATQRSQCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSASYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIEVIMG GGRKYMFPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
NLIDIWKSFK PKHKHSHYVW NRTDLLALDP HSVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNATDPSLS EMVEMAIRIL NKNPKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDQAIG QAGAMTSVED TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVGGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYAACI GANRDHCASA
510 520
SSSGSPSPGP LLLLLALLPL GSLF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 210 | H → Y in AAA30380 (PubMed:3436527).Curated | 1 | |
Sequence conflicti | 475 | H → Q in AAA30380 (PubMed:3436527).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18443 mRNA Translation: AAA30380.1 BC118208 mRNA Translation: AAI18209.1 |
PIRi | A29600 |
RefSeqi | NP_789828.2, NM_176858.2 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18443 mRNA Translation: AAA30380.1 BC118208 mRNA Translation: AAI18209.1 |
PIRi | A29600 |
RefSeqi | NP_789828.2, NM_176858.2 |
3D structure databases
SMRi | P09487 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000011783 |
Chemistry databases
BindingDBi | P09487 |
ChEMBLi | CHEMBL2406894 |
DrugCentrali | P09487 |
Proteomic databases
PaxDbi | P09487 |
PRIDEi | P09487 |
Genome annotation databases
Organism-specific databases
CTDi | 249 |
VGNCi | VGNC:25850, ALPL |
Phylogenomic databases
eggNOGi | KOG4126, Eukaryota |
GeneTreei | ENSGT00950000183063 |
HOGENOMi | CLU_008539_4_0_1 |
InParanoidi | P09487 |
OMAi | IDMAHHA |
OrthoDBi | 416703at2759 |
TreeFami | TF323513 |
Enzyme and pathway databases
BRENDAi | 3.1.3.1, 908 |
Miscellaneous databases
PROi | PR:P09487 |
Gene expression databases
Bgeei | ENSBTAG00000008951, Expressed in adult mammalian kidney and 17 other tissues |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPBT_BOVIN | |
Accessioni | P09487Primary (citable) accession number: P09487 Secondary accession number(s): Q17QS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | May 1, 2007 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families