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Protein

SPARC

Gene

SPARC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi274 – 285Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • collagen binding Source: UniProtKB
  • extracellular matrix binding Source: GO_Central

GO - Biological processi

Keywordsi

LigandCalcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation
R-HSA-3000178 ECM proteoglycans
R-HSA-3000497 Scavenging by Class H Receptors
SIGNORiP09486

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name:
BM-40
Osteonectin
Short name:
ON
Secreted protein acidic and rich in cysteine
Gene namesi
Name:SPARC
Synonyms:ON
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113140.10
HGNCiHGNC:11219 SPARC
MIMi182120 gene
neXtProtiNX_P09486

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Osteogenesis imperfecta 17 (OI17)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae.
See also OMIM:616507
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_075142166R → H in OI17; decreased secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517662Ensembl.1
Natural variantiVAR_075143263E → K in OI17; no effect on expression and secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517663Ensembl.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166R → A, L or K: Strongly reduced collagen binding. 1 Publication1
Mutagenesisi173N → A or Q: Strongly reduced collagen binding. 1 Publication1
Mutagenesisi259L → A: Loss of collagen binding. 1 Publication1
Mutagenesisi262M → A: Strongly reduced collagen binding. 1 Publication1
Mutagenesisi263E → A: Loss of collagen binding. 1 Publication1

Keywords - Diseasei

Disease mutation, Osteogenesis imperfecta

Organism-specific databases

DisGeNETi6678
MalaCardsiSPARC
MIMi616507 phenotype
OpenTargetsiENSG00000113140
PharmGKBiPA36055

Polymorphism and mutation databases

BioMutaiSPARC
DMDMi129283

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 172 PublicationsAdd BLAST17
ChainiPRO_000002030418 – 303SPARCAdd BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi72 ↔ 83PROSITE-ProRule annotation1 Publication
Disulfide bondi77 ↔ 93PROSITE-ProRule annotation1 Publication
Disulfide bondi95 ↔ 130PROSITE-ProRule annotation1 Publication
Disulfide bondi101 ↔ 123PROSITE-ProRule annotation1 Publication
Disulfide bondi112 ↔ 149PROSITE-ProRule annotation1 Publication
Glycosylationi116N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi155 ↔ 265PROSITE-ProRule annotation1 Publication
Disulfide bondi273 ↔ 289PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP09486
MaxQBiP09486
PaxDbiP09486
PeptideAtlasiP09486
PRIDEiP09486
ProteomicsDBi52226

2D gel databases

OGPiP09486

PTM databases

GlyConnecti706
iPTMnetiP09486
PhosphoSitePlusiP09486
UniCarbKBiP09486

Miscellaneous databases

PMAP-CutDBiP09486

Expressioni

Developmental stagei

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Gene expression databases

BgeeiENSG00000113140
CleanExiHS_SPARC
ExpressionAtlasiP09486 baseline and differential
GenevisibleiP09486 HS

Organism-specific databases

HPAiCAB002306
HPA002989
HPA003020

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi112560, 10 interactors
DIPiDIP-46426N
IntActiP09486, 14 interactors
MINTiP09486
STRINGi9606.ENSP00000231061

Structurei

Secondary structure

1303
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni71 – 74Combined sources4
Beta strandi81 – 85Combined sources5
Beta strandi91 – 95Combined sources5
Helixi98 – 100Combined sources3
Helixi107 – 109Combined sources3
Beta strandi111 – 113Combined sources3
Turni114 – 116Combined sources3
Beta strandi118 – 121Combined sources4
Helixi122 – 131Combined sources10
Turni132 – 134Combined sources3
Helixi136 – 140Combined sources5
Beta strandi143 – 148Combined sources6
Helixi157 – 181Combined sources25
Beta strandi183 – 188Combined sources6
Helixi190 – 201Combined sources12
Helixi213 – 222Combined sources10
Helixi224 – 227Combined sources4
Helixi228 – 238Combined sources11
Beta strandi244 – 247Combined sources4
Turni249 – 252Combined sources4
Helixi253 – 256Combined sources4
Turni257 – 259Combined sources3
Helixi260 – 262Combined sources3
Helixi263 – 265Combined sources3
Helixi266 – 273Combined sources8
Beta strandi278 – 282Combined sources5
Helixi283 – 289Combined sources7
Helixi294 – 296Combined sources3
Helixi299 – 301Combined sources3

3D structure databases

ProteinModelPortaliP09486
SMRiP09486
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09486

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 93Follistatin-likeAdd BLAST23
Domaini89 – 151Kazal-likePROSITE-ProRule annotationAdd BLAST63
Domaini261 – 296EF-handAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 69Asp/Glu-rich (acidic; binds calcium)Add BLAST48

Sequence similaritiesi

Belongs to the SPARC family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4004 Eukaryota
ENOG41101WW LUCA
GeneTreeiENSGT00510000046787
HOVERGENiHBG002746
InParanoidiP09486
OMAiKDFPRRM
OrthoDBiEOG091G0CPG
PhylomeDBiP09486
TreeFamiTF319356

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR003645 Fol_N
IPR015369 Follistatin/Osteonectin_EGF
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR001999 Osteonectin_CS
IPR037641 SPARC
IPR019577 SPARC/Testican_Ca-bd-dom
PANTHERiPTHR13866 PTHR13866, 1 hit
PfamiView protein in Pfam
PF09289 FOLN, 1 hit
PF00050 Kazal_1, 1 hit
PF10591 SPARC_Ca_bdg, 1 hit
SMARTiView protein in SMART
SM00274 FOLN, 1 hit
SM00280 KAZAL, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS51465 KAZAL_2, 1 hit
PS00612 OSTEONECTIN_1, 1 hit
PS00613 OSTEONECTIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ QEALPDETEV VEETVAEVTE VSVGANPVQV
60 70 80 90 100
EVGEFDDGAE ETEEEVVAEN PCQNHHCKHG KVCELDENNT PMCVCQDPTS
110 120 130 140 150
CPAPIGEFEK VCSNDNKTFD SSCHFFATKC TLEGTKKGHK LHLDYIGPCK
160 170 180 190 200
YIPPCLDSEL TEFPLRMRDW LKNVLVTLYE RDEDNNLLTE KQKLRVKKIH
210 220 230 240 250
ENEKRLEAGD HPVELLARDF EKNYNMYIFP VHWQFGQLDQ HPIDGYLSHT
260 270 280 290 300
ELAPLRAPLI PMEHCTTRFF ETCDLDNDKY IALDEWAGCF GIKQKDIDKD

LVI
Length:303
Mass (Da):34,632
Last modified:July 1, 1989 - v1
Checksum:iB914599F79705945
GO

Sequence cautioni

The sequence AAA60993 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42 – 43SV → PT AA sequence (PubMed:3597437).Curated2
Sequence conflicti205R → L in AAA60993 (PubMed:2790009).Curated1
Sequence conflicti269F → L in AAA60993 (PubMed:2790009).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05043119P → S. Corresponds to variant dbSNP:rs6874468Ensembl.1
Natural variantiVAR_05953070N → S. Corresponds to variant dbSNP:rs13359508Ensembl.1
Natural variantiVAR_075142166R → H in OI17; decreased secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517662Ensembl.1
Natural variantiVAR_075143263E → K in OI17; no effect on expression and secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517663Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00755 mRNA Translation: CAA68724.1
J03040 mRNA Translation: AAA60570.1
M25746
, M25738, M25739, M25740, M25741, M25742, M25743, M25744, M25745 Genomic DNA Translation: AAA60993.1 Sequence problems.
CR456799 mRNA Translation: CAG33080.1
CH471062 Genomic DNA Translation: EAW61668.1
CH471062 Genomic DNA Translation: EAW61669.1
CH471062 Genomic DNA Translation: EAW61670.1
CH471062 Genomic DNA Translation: EAW61672.1
BC004974 mRNA Translation: AAH04974.1
BC008011 mRNA Translation: AAH08011.1
BC072457 mRNA Translation: AAH72457.1
AL709729 mRNA No translation available.
CCDSiCCDS4318.1
PIRiA32821 GEHUN
RefSeqiNP_001296372.1, NM_001309443.1
NP_001296373.1, NM_001309444.1
NP_003109.1, NM_003118.3
UniGeneiHs.111779

Genome annotation databases

EnsembliENST00000231061; ENSP00000231061; ENSG00000113140
GeneIDi6678
KEGGihsa:6678
UCSCiuc003lui.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSPRC_HUMAN
AccessioniPrimary (citable) accession number: P09486
Secondary accession number(s): D3DQH9, Q6IBK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 18, 2018
This is version 199 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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