UniProtKB - P09486 (SPRC_HUMAN)
Protein
SPARC
Gene
SPARC
Organism
Homo sapiens (Human)
Status
Functioni
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 274 – 285 | Add BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- collagen binding Source: UniProtKB
- extracellular matrix binding Source: GO_Central
GO - Biological processi
- anatomical structure development Source: GO_Central
- bone development Source: Ensembl
- cellular response to growth factor stimulus Source: Ensembl
- extracellular matrix organization Source: Reactome
- heart development Source: Ensembl
- inner ear development Source: Ensembl
- lung development Source: Ensembl
- negative regulation of angiogenesis Source: UniProtKB
- negative regulation of endothelial cell proliferation Source: UniProtKB
- ossification Source: Ensembl
- pigmentation Source: Ensembl
- platelet degranulation Source: Reactome
- positive regulation of endothelial cell migration Source: UniProtKB
- receptor-mediated endocytosis Source: Reactome
- regulation of cell morphogenesis Source: UniProtKB
- regulation of synapse organization Source: Ensembl
- response to cadmium ion Source: Ensembl
- response to calcium ion Source: Ensembl
- response to cAMP Source: Ensembl
- response to cytokine Source: Ensembl
- response to ethanol Source: Ensembl
- response to glucocorticoid Source: Ensembl
- response to gravity Source: Ensembl
- response to L-ascorbic acid Source: Ensembl
- response to lead ion Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to peptide hormone Source: Ensembl
- wound healing Source: Ensembl
Keywordsi
Ligand | Calcium, Copper, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | P09486 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-1251985, Nuclear signaling by ERBB4 R-HSA-3000178, ECM proteoglycans R-HSA-3000497, Scavenging by Class H Receptors |
SIGNORi | P09486 |
Names & Taxonomyi
Protein namesi | Recommended name: SPARCAlternative name(s): Basement-membrane protein 40 Short name: BM-40 Osteonectin Short name: ON Secreted protein acidic and rich in cysteine |
Gene namesi | Name:SPARC Synonyms:ON |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11219, SPARC |
MIMi | 182120, gene |
neXtProti | NX_P09486 |
VEuPathDBi | HostDB:ENSG00000113140.10 |
Subcellular locationi
Extracellular region or secreted
- basement membrane 4 Publications
Note: In or around the basement membrane.
Extracellular region or secreted
- basement membrane Source: UniProtKB-SubCell
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular region Source: BHF-UCL
- extracellular space Source: GO_Central
Nucleus
- nuclear matrix Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
- cytoplasm Source: UniProtKB
- endocytic vesicle lumen Source: Reactome
- glutamatergic synapse Source: Ensembl
- intracellular membrane-bounded organelle Source: HPA
- platelet alpha granule Source: UniProtKB
- platelet alpha granule lumen Source: Reactome
- platelet alpha granule membrane Source: UniProtKB
Keywords - Cellular componenti
Basement membrane, Extracellular matrix, SecretedPathology & Biotechi
Involvement in diseasei
Osteogenesis imperfecta 17 (OI17)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_075142 | 166 | R → H in OI17; decreased secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517662EnsemblClinVar. | 1 | |
Natural variantiVAR_075143 | 263 | E → K in OI17; no effect on expression and secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517663EnsemblClinVar. | 1 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 166 | R → A, L or K: Strongly reduced collagen binding. 1 Publication | 1 | |
Mutagenesisi | 173 | N → A or Q: Strongly reduced collagen binding. 1 Publication | 1 | |
Mutagenesisi | 259 | L → A: Loss of collagen binding. 1 Publication | 1 | |
Mutagenesisi | 262 | M → A: Strongly reduced collagen binding. 1 Publication | 1 | |
Mutagenesisi | 263 | E → A: Loss of collagen binding. 1 Publication | 1 |
Keywords - Diseasei
Disease variant, Osteogenesis imperfectaOrganism-specific databases
DisGeNETi | 6678 |
MalaCardsi | SPARC |
MIMi | 616507, phenotype |
OpenTargetsi | ENSG00000113140 |
Orphaneti | 216820, Osteogenesis imperfecta type 4 |
PharmGKBi | PA36055 |
Miscellaneous databases
Pharosi | P09486, Tbio |
Chemistry databases
DrugBanki | DB11093, Calcium citrate DB11348, Calcium Phosphate DB14481, Calcium phosphate dihydrate |
Genetic variation databases
BioMutai | SPARC |
DMDMi | 129283 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | 2 PublicationsAdd BLAST | 17 | |
ChainiPRO_0000020304 | 18 – 303 | SPARCAdd BLAST | 286 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 72 ↔ 83 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 77 ↔ 93 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 95 ↔ 130 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 101 ↔ 123 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 112 ↔ 149 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 116 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 155 ↔ 265 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 273 ↔ 289 | PROSITE-ProRule annotation1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | non-CPTAC-2699 |
EPDi | P09486 |
jPOSTi | P09486 |
MassIVEi | P09486 |
MaxQBi | P09486 |
PaxDbi | P09486 |
PeptideAtlasi | P09486 |
PRIDEi | P09486 |
ProteomicsDBi | 52226 |
2D gel databases
OGPi | P09486 |
PTM databases
GlyConnecti | 706, 37 N-Linked glycans (1 site) |
GlyGeni | P09486, 2 sites, 9 N-linked glycans (1 site), 1 O-linked glycan (1 site) |
iPTMneti | P09486 |
MetOSitei | P09486 |
PhosphoSitePlusi | P09486 |
Expressioni
Developmental stagei
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Gene expression databases
Bgeei | ENSG00000113140, Expressed in tibia and 242 other tissues |
ExpressionAtlasi | P09486, baseline and differential |
Genevisiblei | P09486, HS |
Organism-specific databases
HPAi | ENSG00000113140, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsP09486
With | #Exp. | IntAct |
---|---|---|
COL3A1 - isoform 1 [P02461-1] | 4 | EBI-2800983,EBI-15740444 |
MYOC [Q99972] | 3 | EBI-2800983,EBI-11692272 |
faf [Q2TV77] from Finegoldia magna. | 4 | EBI-2800983,EBI-6405263 |
Protein-protein interaction databases
BioGRIDi | 112560, 36 interactors |
DIPi | DIP-46426N |
IntActi | P09486, 21 interactors |
MINTi | P09486 |
STRINGi | 9606.ENSP00000231061 |
Miscellaneous databases
RNActi | P09486, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09486 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09486 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 71 – 93 | Follistatin-likeAdd BLAST | 23 | |
Domaini | 89 – 151 | Kazal-likePROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 261 – 296 | EF-handAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 69 | Asp/Glu-rich (acidic; binds calcium)Add BLAST | 48 |
Sequence similaritiesi
Belongs to the SPARC family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4004, Eukaryota |
GeneTreei | ENSGT00510000046787 |
HOGENOMi | CLU_047416_0_0_1 |
InParanoidi | P09486 |
OMAi | LVEWGAC |
OrthoDBi | 1528521at2759 |
PhylomeDBi | P09486 |
TreeFami | TF319356 |
Family and domain databases
IDEALi | IID00648 |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P09486-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRAWIFFLLC LAGRALAAPQ QEALPDETEV VEETVAEVTE VSVGANPVQV
60 70 80 90 100
EVGEFDDGAE ETEEEVVAEN PCQNHHCKHG KVCELDENNT PMCVCQDPTS
110 120 130 140 150
CPAPIGEFEK VCSNDNKTFD SSCHFFATKC TLEGTKKGHK LHLDYIGPCK
160 170 180 190 200
YIPPCLDSEL TEFPLRMRDW LKNVLVTLYE RDEDNNLLTE KQKLRVKKIH
210 220 230 240 250
ENEKRLEAGD HPVELLARDF EKNYNMYIFP VHWQFGQLDQ HPIDGYLSHT
260 270 280 290 300
ELAPLRAPLI PMEHCTTRFF ETCDLDNDKY IALDEWAGCF GIKQKDIDKD
LVI
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GY03 | F5GY03_HUMAN | Osteonectin | SPARC | 149 | Annotation score: | ||
E5RK62 | E5RK62_HUMAN | Osteonectin | SPARC | 115 | Annotation score: | ||
F5H4E2 | F5H4E2_HUMAN | SPARC | SPARC | 111 | Annotation score: | ||
E5RJA5 | E5RJA5_HUMAN | SPARC | SPARC | 53 | Annotation score: |
Sequence cautioni
The sequence AAA60993 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 – 43 | SV → PT AA sequence (PubMed:3597437).Curated | 2 | |
Sequence conflicti | 205 | R → L in AAA60993 (PubMed:2790009).Curated | 1 | |
Sequence conflicti | 269 | F → L in AAA60993 (PubMed:2790009).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050431 | 19 | P → S. Corresponds to variant dbSNP:rs6874468Ensembl. | 1 | |
Natural variantiVAR_059530 | 70 | N → S. Corresponds to variant dbSNP:rs13359508EnsemblClinVar. | 1 | |
Natural variantiVAR_075142 | 166 | R → H in OI17; decreased secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517662EnsemblClinVar. | 1 | |
Natural variantiVAR_075143 | 263 | E → K in OI17; no effect on expression and secretion of the protein; altered secretion of procollagen type I. 1 PublicationCorresponds to variant dbSNP:rs1057517663EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00755 mRNA Translation: CAA68724.1 J03040 mRNA Translation: AAA60570.1 M25746 , M25738, M25739, M25740, M25741, M25742, M25743, M25744, M25745 Genomic DNA Translation: AAA60993.1 Sequence problems. CR456799 mRNA Translation: CAG33080.1 CH471062 Genomic DNA Translation: EAW61668.1 CH471062 Genomic DNA Translation: EAW61669.1 CH471062 Genomic DNA Translation: EAW61670.1 CH471062 Genomic DNA Translation: EAW61672.1 BC004974 mRNA Translation: AAH04974.1 BC008011 mRNA Translation: AAH08011.1 BC072457 mRNA Translation: AAH72457.1 AL709729 mRNA No translation available. |
CCDSi | CCDS4318.1 |
PIRi | A32821, GEHUN |
RefSeqi | NP_001296372.1, NM_001309443.1 NP_001296373.1, NM_001309444.1 NP_003109.1, NM_003118.3 |
Genome annotation databases
Ensembli | ENST00000231061; ENSP00000231061; ENSG00000113140 |
GeneIDi | 6678 |
KEGGi | hsa:6678 |
UCSCi | uc003lui.5, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Osteonectin entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00755 mRNA Translation: CAA68724.1 J03040 mRNA Translation: AAA60570.1 M25746 , M25738, M25739, M25740, M25741, M25742, M25743, M25744, M25745 Genomic DNA Translation: AAA60993.1 Sequence problems. CR456799 mRNA Translation: CAG33080.1 CH471062 Genomic DNA Translation: EAW61668.1 CH471062 Genomic DNA Translation: EAW61669.1 CH471062 Genomic DNA Translation: EAW61670.1 CH471062 Genomic DNA Translation: EAW61672.1 BC004974 mRNA Translation: AAH04974.1 BC008011 mRNA Translation: AAH08011.1 BC072457 mRNA Translation: AAH72457.1 AL709729 mRNA No translation available. |
CCDSi | CCDS4318.1 |
PIRi | A32821, GEHUN |
RefSeqi | NP_001296372.1, NM_001309443.1 NP_001296373.1, NM_001309444.1 NP_003109.1, NM_003118.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BMO | X-ray | 3.10 | A/B | 71-303 | [»] | |
1NUB | X-ray | 2.80 | A/B | 71-303 | [»] | |
1SRA | X-ray | 2.00 | A | 153-303 | [»] | |
2V53 | X-ray | 3.20 | A | 70-303 | [»] | |
SMRi | P09486 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112560, 36 interactors |
DIPi | DIP-46426N |
IntActi | P09486, 21 interactors |
MINTi | P09486 |
STRINGi | 9606.ENSP00000231061 |
Chemistry databases
DrugBanki | DB11093, Calcium citrate DB11348, Calcium Phosphate DB14481, Calcium phosphate dihydrate |
PTM databases
GlyConnecti | 706, 37 N-Linked glycans (1 site) |
GlyGeni | P09486, 2 sites, 9 N-linked glycans (1 site), 1 O-linked glycan (1 site) |
iPTMneti | P09486 |
MetOSitei | P09486 |
PhosphoSitePlusi | P09486 |
Genetic variation databases
BioMutai | SPARC |
DMDMi | 129283 |
2D gel databases
OGPi | P09486 |
Proteomic databases
CPTACi | non-CPTAC-2699 |
EPDi | P09486 |
jPOSTi | P09486 |
MassIVEi | P09486 |
MaxQBi | P09486 |
PaxDbi | P09486 |
PeptideAtlasi | P09486 |
PRIDEi | P09486 |
ProteomicsDBi | 52226 |
Protocols and materials databases
Antibodypediai | 878, 758 antibodies |
DNASUi | 6678 |
Genome annotation databases
Ensembli | ENST00000231061; ENSP00000231061; ENSG00000113140 |
GeneIDi | 6678 |
KEGGi | hsa:6678 |
UCSCi | uc003lui.5, human |
Organism-specific databases
CTDi | 6678 |
DisGeNETi | 6678 |
GeneCardsi | SPARC |
HGNCi | HGNC:11219, SPARC |
HPAi | ENSG00000113140, Low tissue specificity |
MalaCardsi | SPARC |
MIMi | 182120, gene 616507, phenotype |
neXtProti | NX_P09486 |
OpenTargetsi | ENSG00000113140 |
Orphaneti | 216820, Osteogenesis imperfecta type 4 |
PharmGKBi | PA36055 |
VEuPathDBi | HostDB:ENSG00000113140.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4004, Eukaryota |
GeneTreei | ENSGT00510000046787 |
HOGENOMi | CLU_047416_0_0_1 |
InParanoidi | P09486 |
OMAi | LVEWGAC |
OrthoDBi | 1528521at2759 |
PhylomeDBi | P09486 |
TreeFami | TF319356 |
Enzyme and pathway databases
PathwayCommonsi | P09486 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-1251985, Nuclear signaling by ERBB4 R-HSA-3000178, ECM proteoglycans R-HSA-3000497, Scavenging by Class H Receptors |
SIGNORi | P09486 |
Miscellaneous databases
BioGRID-ORCSi | 6678, 2 hits in 878 CRISPR screens |
ChiTaRSi | SPARC, human |
EvolutionaryTracei | P09486 |
GeneWikii | Osteonectin |
GenomeRNAii | 6678 |
Pharosi | P09486, Tbio |
PROi | PR:P09486 |
RNActi | P09486, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000113140, Expressed in tibia and 242 other tissues |
ExpressionAtlasi | P09486, baseline and differential |
Genevisiblei | P09486, HS |
Family and domain databases
IDEALi | IID00648 |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPRC_HUMAN | |
Accessioni | P09486Primary (citable) accession number: P09486 Secondary accession number(s): D3DQH9, Q6IBK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 219 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families