UniProtKB - P09483 (ACHA4_RAT)
Neuronal acetylcholine receptor subunit alpha-4
Chrna4
Functioni
Miscellaneous
GO - Molecular functioni
- acetylcholine binding Source: RGD
- acetylcholine-gated cation-selective channel activity Source: RGD
- acetylcholine receptor activity Source: RGD
- ion channel activity Source: RGD
- neurotransmitter receptor activity Source: GO_Central
- protein-containing complex binding Source: RGD
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: SynGO
GO - Biological processi
- acetylcholine receptor signaling pathway Source: RGD
- action potential Source: RGD
- B cell activation Source: RGD
- behavioral response to nicotine Source: RGD
- calcium ion transport Source: RGD
- chemical synaptic transmission Source: GO_Central
- cognition Source: RGD
- DNA repair Source: RGD
- exploration behavior Source: RGD
- inhibitory postsynaptic potential Source: RGD
- ion transmembrane transport Source: GO_Central
- locomotory behavior Source: RGD
- membrane depolarization Source: RGD
- nervous system process Source: RGD
- regulation of acetylcholine-gated cation channel activity Source: RGD
- regulation of dopamine secretion Source: RGD
- regulation of membrane potential Source: RGD
- regulation of synaptic vesicle exocytosis Source: RGD
- respiratory gaseous exchange by respiratory system Source: RGD
- response to hypoxia Source: RGD
- response to nicotine Source: RGD
- response to oxidative stress Source: RGD
- sensory perception of pain Source: RGD
- signal transduction Source: RGD
- synaptic transmission, cholinergic Source: RGD
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors R-RNO-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-RNO-629597, Highly calcium permeable nicotinic acetylcholine receptors |
Names & Taxonomyi
Protein namesi | Recommended name: Neuronal acetylcholine receptor subunit alpha-4 |
Gene namesi | Name:Chrna4 Synonyms:Acra4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2346, Chrna4 |
Subcellular locationi
Plasma membrane
- postsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Plasma Membrane
- acetylcholine-gated channel complex Source: RGD
- external side of plasma membrane Source: RGD
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic specialization membrane Source: SynGO
- integral component of presynaptic membrane Source: SynGO
- plasma membrane Source: RGD
Other locations
- cholinergic synapse Source: SynGO
- dendrite Source: RGD
- dopaminergic synapse Source: RGD
- membrane Source: RGD
- neuron projection Source: GO_Central
- neuronal cell body Source: RGD
- protein-containing complex Source: RGD
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 32 – 249 | ExtracellularSequence analysisAdd BLAST | 218 | |
Transmembranei | 250 – 270 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 279 – 299 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 313 – 333 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 334 – 604 | CytoplasmicSequence analysisAdd BLAST | 271 | |
Transmembranei | 605 – 625 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 219 – 240 | TYNTR…TYAFI → YKHDIKYNCCEEIYTDITYS FY: >10000-fold increase in inhibition of alpha-4-beta-2 nAChR by the conotoxin BuIA. 1 PublicationAdd BLAST | 22 | |
Mutagenesisi | 220 – 223 | YNTR → KHDI: >1700-fold increase in inhibition of alpha-4-beta-2 nAChR by the conotoxin BuIA. 1 Publication | 4 | |
Mutagenesisi | 223 | R → I: >10-fold increase in inhibition of alpha-4-beta-2 nAChR by the conotoxin BuIA. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL307 |
DrugCentrali | P09483 |
GuidetoPHARMACOLOGYi | 465 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 30 | Sequence analysisAdd BLAST | 30 | |
ChainiPRO_0000000354 | 31 – 630 | Neuronal acetylcholine receptor subunit alpha-4Add BLAST | 600 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 59 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 109 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 163 ↔ 177 | By similarity | ||
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 227 ↔ 228 | Associated with receptor activationBy similarity | ||
Lipidationi | 273 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 428 | PhosphoserineCombined sources | 1 | |
Modified residuei | 542 | PhosphoserineBy similarity | 1 | |
Modified residuei | 545 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P09483 |
PRIDEi | P09483 |
PTM databases
GlyGeni | P09483, 3 sites |
iPTMneti | P09483 |
PhosphoSitePlusi | P09483 |
SwissPalmi | P09483 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers.
Interacts with RIC3; which is required for proper folding and assembly.
Interacts with LYPD6. The heteropentamer alpha-4-beta-2 interacts with alpha-conotoxins PnIA, GID and MII (PubMed:15929983).
By similarity1 PublicationBinary interactionsi
Hide detailsP09483
With | #Exp. | IntAct |
---|---|---|
Chrna5 [P20420] | 6 | EBI-7842410,EBI-10828372 |
Chrnb2 [P12390] | 6 | EBI-7842410,EBI-9008812 |
Chrnb4 [P12392] | 4 | EBI-7842410,EBI-9008856 |
Protein-protein interaction databases
ComplexPortali | CPX-170, Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2 CPX-171, Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2 CPX-215, Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2 CPX-219, Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4 |
CORUMi | P09483 |
IntActi | P09483, 6 interactors |
MINTi | P09483 |
STRINGi | 10116.ENSRNOP00000009041 |
Chemistry databases
BindingDBi | P09483 |
Structurei
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3645, Eukaryota |
InParanoidi | P09483 |
OrthoDBi | 381858at2759 |
PhylomeDBi | P09483 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MANSGTGAPP PLLLLPLLLL LGTGLLPASS HIETRAHAEE RLLKRLFSGY
60 70 80 90 100
NKWSRPVANI SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW VKQEWHDYKL
110 120 130 140 150
RWDPGDYENV TSIRIPSELI WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR
160 170 180 190 200
VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK FGSWTYDKAK IDLVSMHSRV
210 220 230 240 250
DQLDFWESGE WVIVDAVGTY NTRKYECCAE IYPDITYAFI IRRLPLFYTI
260 270 280 290 300
NLIIPCLLIS CLTVLVFYLP SECGEKVTLC ISVLLSLTVF LLLITEIIPS
310 320 330 340 350
PTSLVIPLIG EYLLFTMIFV TLSIVITVFV LNVHHRSPRT HTMPAWVRRV
360 370 380 390 400
FLDIVPRLLF MKRPSVVKDN CRRLIESMHK MANAPRFWPE PVGEPGILSD
410 420 430 440 450
ICNQGLSPAP TFCNPTDTAV ETQPTCRSPP LEVPDLKTSE VEKASPCPSP
460 470 480 490 500
GSCPPPKSSS GAPMLIKARS LSVQHVPSSQ EAAEDGIRCR SRSIQYCVSQ
510 520 530 540 550
DGAASLADSK PTSSPTSLKA RPSQLPVSDQ ASPCKCTCKE PSPVSPVTVL
560 570 580 590 600
KAGGTKAPPQ HLPLSPALTR AVEGVQYIAD HLKAEDTDFS VKEDWKYVAM
610 620 630
VIDRIFLWMF IIVCLLGTVG LFLPPWLAAC
The sequence of this isoform differs from the canonical sequence as follows:
629-630: AC → GMI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K6W5 | A0A0G2K6W5_RAT | Cholinergic receptor, nicotinic, al... | Chrna4 rCG_38452 | 630 | Annotation score: | ||
K4DIC3 | K4DIC3_RAT | Neuronal acetylcholine receptor sub... | Chrna4 | 626 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 4 | MANS → MEIGGPGA in AAA41676 (PubMed:3829125).Curated | 4 | |
Sequence conflicti | 6 | T → P in AAB64439 (Ref. 3) Curated | 1 | |
Sequence conflicti | 58 | A → G in AAA41676 (PubMed:3829125).Curated | 1 | |
Sequence conflicti | 58 | A → G in AAB64439 (Ref. 3) Curated | 1 | |
Sequence conflicti | 196 | M → I in AAA41676 (PubMed:3829125).Curated | 1 | |
Sequence conflicti | 196 | M → I in AAB64439 (Ref. 3) Curated | 1 | |
Sequence conflicti | 301 | Missing in AAA41676 (PubMed:3829125).Curated | 1 | |
Sequence conflicti | 301 | Missing in AAC97071 (PubMed:3829125).Curated | 1 | |
Sequence conflicti | 301 | Missing in AAB64439 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000074 | 629 – 630 | AC → GMI in isoform Alpha-4-2. 2 Publications | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15682 mRNA Translation: AAA41676.1 L31620 mRNA Translation: AAC97071.1 AF007212 mRNA Translation: AAB64439.1 |
RefSeqi | NP_077330.1, NM_024354.1 |
Genome annotation databases
GeneIDi | 25590 |
KEGGi | rno:25590 |
UCSCi | RGD:2346, rat [P09483-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15682 mRNA Translation: AAA41676.1 L31620 mRNA Translation: AAC97071.1 AF007212 mRNA Translation: AAB64439.1 |
RefSeqi | NP_077330.1, NM_024354.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OLE | model | - | A/C | 38-245 | [»] | |
ModBasei | Search... | |||||
SWISS-MODEL-Workspacei | Submit a new modelling project... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-170, Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2 CPX-171, Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2 CPX-215, Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2 CPX-219, Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4 |
CORUMi | P09483 |
IntActi | P09483, 6 interactors |
MINTi | P09483 |
STRINGi | 10116.ENSRNOP00000009041 |
Chemistry databases
BindingDBi | P09483 |
ChEMBLi | CHEMBL307 |
DrugCentrali | P09483 |
GuidetoPHARMACOLOGYi | 465 |
PTM databases
GlyGeni | P09483, 3 sites |
iPTMneti | P09483 |
PhosphoSitePlusi | P09483 |
SwissPalmi | P09483 |
Proteomic databases
PaxDbi | P09483 |
PRIDEi | P09483 |
Genome annotation databases
GeneIDi | 25590 |
KEGGi | rno:25590 |
UCSCi | RGD:2346, rat [P09483-1] |
Organism-specific databases
CTDi | 1137 |
RGDi | 2346, Chrna4 |
Phylogenomic databases
eggNOGi | KOG3645, Eukaryota |
InParanoidi | P09483 |
OrthoDBi | 381858at2759 |
PhylomeDBi | P09483 |
Enzyme and pathway databases
Reactomei | R-RNO-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors R-RNO-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-RNO-629597, Highly calcium permeable nicotinic acetylcholine receptors |
Miscellaneous databases
PROi | PR:P09483 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACHA4_RAT | |
Accessioni | P09483Primary (citable) accession number: P09483 Secondary accession number(s): O35769 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 10, 2021 | |
This is version 183 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families