UniProtKB - P09455 (RET1_HUMAN)
Retinol-binding protein 1
RBP1
Functioni
Cytoplasmic retinol-binding protein (PubMed:22665496, PubMed:26900151, PubMed:28057518).
Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed:15632377, PubMed:22665496).
4 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 41 | All-trans retinolCombined sources1 Publication | 1 | |
Binding sitei | 63 | All-trans retinolCombined sources1 Publication | 1 | |
Binding sitei | 109 | All-trans retinolCombined sources2 Publications | 1 |
GO - Molecular functioni
- all-trans-retinol binding Source: UniProtKB
- fatty acid binding Source: GO_Central
- retinal binding Source: UniProtKB-KW
- retinoid binding Source: ProtInc
GO - Biological processi
- fatty acid transport Source: GO_Central
- lipid homeostasis Source: UniProtKB
- retinoic acid biosynthetic process Source: InterPro
- vitamin A metabolic process Source: UniProtKB
Keywordsi
Biological process | Transport |
Ligand | Retinol-binding, Vitamin A |
Enzyme and pathway databases
PathwayCommonsi | P09455 |
Reactomei | R-HSA-2453864, Retinoid cycle disease events R-HSA-2453902, The canonical retinoid cycle in rods (twilight vision) R-HSA-975634, Retinoid metabolism and transport |
SignaLinki | P09455 |
SIGNORi | P09455 |
Names & Taxonomyi
Protein namesi | Recommended name: Retinol-binding protein 1Alternative name(s): Cellular retinol-binding protein Short name: CRBP Cellular retinol-binding protein I Short name: CRBP-I |
Gene namesi | Name:RBP1 Synonyms:CRBP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9919, RBP1 |
MIMi | 180260, gene |
neXtProti | NX_P09455 |
VEuPathDBi | HostDB:ENSG00000114115 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Lipid droplet 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- lipid droplet Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Lipid dropletPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 22 | R → A: No effect on retinol binding. Abolishes interaction with STA6 and the ability to enhance STA6-mediated vitamin A uptake. 1 Publication | 1 | |
Mutagenesisi | 30 | L → A: Decreases cellular retinol uptake and impairs retinol storage. 1 Publication | 1 | |
Mutagenesisi | 31 | R → A: No effect on retinol binding. Abolishes interaction with STA6 and the ability to enhance STA6-mediated vitamin A uptake. 1 Publication | 1 | |
Mutagenesisi | 32 | K → A: No effect on retinol binding. Abolishes interaction with STA6 and the ability to enhance STA6-mediated vitamin A uptake. 1 Publication | 1 | |
Mutagenesisi | 41 | K → L: Strongly decreased affinity for retinol. Further decrease in affinity for retinol; when associated with L-109. 1 Publication | 1 | |
Mutagenesisi | 59 | R → E: Decreases cellular retinol uptake and impairs retinol storage. 1 Publication | 1 | |
Mutagenesisi | 109 | Q → L: Strongly decreased affinity for retinol. Further decrease in for retinol; when associated with L-41. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5947 |
OpenTargetsi | ENSG00000114115 |
PharmGKBi | PA34286 |
Miscellaneous databases
Pharosi | P09455, Tbio |
Chemistry databases
DrugBanki | DB00459, Acitretin DB06755, Beta carotene DB00162, Vitamin A |
DrugCentrali | P09455 |
Genetic variation databases
BioMutai | RBP1 |
DMDMi | 132387 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000067392 | 1 – 135 | Retinol-binding protein 1Add BLAST | 135 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Isoform 2 (identifier: P09455-2) | |||||
Modified residuei | 9 | Omega-N-methylarginineCombined sources | 1 | ||
Isoform 3 (identifier: P09455-3) | |||||
Modified residuei | 9 | Omega-N-methylarginineCombined sources | 1 |
Keywords - PTMi
MethylationProteomic databases
EPDi | P09455 |
jPOSTi | P09455 |
MassIVEi | P09455 |
MaxQBi | P09455 |
PaxDbi | P09455 |
PeptideAtlasi | P09455 |
PRIDEi | P09455 |
ProteomicsDBi | 18915 23698 52219 [P09455-1] |
PTM databases
iPTMneti | P09455 |
PhosphoSitePlusi | P09455 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000114115, Expressed in pigmented layer of retina and 222 other tissues |
ExpressionAtlasi | P09455, baseline and differential |
Genevisiblei | P09455, HS |
Organism-specific databases
HPAi | ENSG00000114115, Tissue enhanced (ovary, pancreas) |
Interactioni
Subunit structurei
Interacts (only as retinol-free apoprotein) with STRA6.
1 PublicationBinary interactionsi
P09455
With | #Exp. | IntAct |
---|---|---|
DHPS [P49366] | 3 | EBI-2623483,EBI-741925 |
TNFRSF10D [Q9UBN6] | 3 | EBI-2623483,EBI-1044859 |
TTC19 [Q6DKK2] | 3 | EBI-2623483,EBI-948354 |
UBE2J2 [Q8N2K1] | 3 | EBI-2623483,EBI-2340110 |
Protein-protein interaction databases
BioGRIDi | 111881, 27 interactors |
IntActi | P09455, 10 interactors |
MINTi | P09455 |
STRINGi | 9606.ENSP00000232219 |
Miscellaneous databases
RNActi | P09455, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P09455 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 32 | Important for interaction with STRA61 PublicationAdd BLAST | 11 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4015, Eukaryota |
GeneTreei | ENSGT00940000159675 |
HOGENOMi | CLU_113772_5_1_1 |
InParanoidi | P09455 |
OrthoDBi | 1377380at2759 |
PhylomeDBi | P09455 |
TreeFami | TF316894 |
Family and domain databases
Gene3Di | 2.40.128.20, 1 hit |
InterProi | View protein in InterPro IPR012674, Calycin IPR031264, CRBP1 IPR000463, Fatty_acid-bd IPR031259, ILBP IPR000566, Lipocln_cytosolic_FA-bd_dom |
PANTHERi | PTHR11955, PTHR11955, 1 hit PTHR11955:SF56, PTHR11955:SF56, 1 hit |
Pfami | View protein in Pfam PF00061, Lipocalin, 1 hit |
PRINTSi | PR00178, FATTYACIDBP |
SUPFAMi | SSF50814, SSF50814, 1 hit |
PROSITEi | View protein in PROSITE PS00214, FABP, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPVDFTGYWK MLVNENFEEY LRALDVNVAL RKIANLLKPD KEIVQDGDHM
60 70 80 90 100
IIRTLSTFRN YIMDFQVGKE FEEDLTGIDD RKCMTTVSWD GDKLQCVQKG
110 120 130
EKEGRGWTQW IEGDELHLEM RVEGVVCKQV FKKVQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MQT0 | A0A0A0MQT0_HUMAN | Retinol binding protein 1, cellular | RBP1 hCG_16055 | 197 | Annotation score: | ||
A0A0A0MTB9 | A0A0A0MTB9_HUMAN | Retinol-binding protein 1 | RBP1 | 91 | Annotation score: | ||
H0YAK8 | H0YAK8_HUMAN | Retinol-binding protein 1 | RBP1 | 98 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Isoform 2 (identifier: P09455-2) | |||||
Sequence conflicti | 18 | P → L in BAH13536 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046201 | 1 | M → MDPPAGFVRAGNPAVAAPQS PLSPEGAHFRAAHHPRSTGS RCPGSLQPSRPLVANWLQSL PEM in isoform 2 and isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_046202 | 85 – 135 | TTVSW…FKKVQ → SETGFSS in isoform 2. 1 PublicationAdd BLAST | 51 | |
Alternative sequenceiVSP_046203 | 85 – 135 | TTVSW…FKKVQ → AGVQSRDLSSL in isoform 3. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11433 mRNA Translation: AAA60257.1 X07437, X07438 Genomic DNA Translation: CAA30318.1 AK290315 mRNA Translation: BAF83004.1 AK301684 mRNA Translation: BAH13536.1 AK309492 Genomic DNA No translation available. CR541979 mRNA Translation: CAG46776.1 CR542005 mRNA Translation: CAG46802.1 AC046134 Genomic DNA No translation available. BC121052 mRNA Translation: AAI21053.1 M36809 mRNA Translation: AAA35714.1 |
CCDSi | CCDS46925.1 [P09455-2] CCDS46926.1 [P09455-3] |
PIRi | S00399, RJHUO |
RefSeqi | NP_001124464.1, NM_001130992.1 [P09455-3] NP_001124465.1, NM_001130993.1 [P09455-2] NP_002890.2, NM_002899.3 |
Genome annotation databases
Ensembli | ENST00000492918; ENSP00000429166; ENSG00000114115 [P09455-3] ENST00000617459; ENSP00000477621; ENSG00000114115 [P09455-2] ENST00000619087; ENSP00000482165; ENSG00000114115 |
GeneIDi | 5947 |
KEGGi | hsa:5947 |
UCSCi | uc011bmx.2, human [P09455-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11433 mRNA Translation: AAA60257.1 X07437, X07438 Genomic DNA Translation: CAA30318.1 AK290315 mRNA Translation: BAF83004.1 AK301684 mRNA Translation: BAH13536.1 AK309492 Genomic DNA No translation available. CR541979 mRNA Translation: CAG46776.1 CR542005 mRNA Translation: CAG46802.1 AC046134 Genomic DNA No translation available. BC121052 mRNA Translation: AAI21053.1 M36809 mRNA Translation: AAA35714.1 |
CCDSi | CCDS46925.1 [P09455-2] CCDS46926.1 [P09455-3] |
PIRi | S00399, RJHUO |
RefSeqi | NP_001124464.1, NM_001130992.1 [P09455-3] NP_001124465.1, NM_001130993.1 [P09455-2] NP_002890.2, NM_002899.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5H8T | X-ray | 1.21 | A | 2-135 | [»] | |
5H9A | X-ray | 1.38 | A | 2-135 | [»] | |
5HA1 | X-ray | 1.35 | A | 2-135 | [»] | |
5HBS | X-ray | 0.89 | A | 2-135 | [»] | |
5LJB | X-ray | 1.26 | A | 1-135 | [»] | |
5LJC | X-ray | 1.43 | A | 1-135 | [»] | |
5LJD | X-ray | 1.61 | A | 1-135 | [»] | |
5LJE | X-ray | 1.40 | A | 1-135 | [»] | |
5LJG | X-ray | 1.15 | A | 1-135 | [»] | |
5LJH | X-ray | 1.52 | A | 1-135 | [»] | |
5LJK | X-ray | 1.70 | A | 1-135 | [»] | |
6E5L | X-ray | 1.17 | A | 2-135 | [»] | |
6E5T | X-ray | 1.55 | A | 2-135 | [»] | |
6E6M | X-ray | 1.55 | A/B/C | 2-135 | [»] | |
SMRi | P09455 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111881, 27 interactors |
IntActi | P09455, 10 interactors |
MINTi | P09455 |
STRINGi | 9606.ENSP00000232219 |
Chemistry databases
DrugBanki | DB00459, Acitretin DB06755, Beta carotene DB00162, Vitamin A |
DrugCentrali | P09455 |
PTM databases
iPTMneti | P09455 |
PhosphoSitePlusi | P09455 |
Genetic variation databases
BioMutai | RBP1 |
DMDMi | 132387 |
Proteomic databases
EPDi | P09455 |
jPOSTi | P09455 |
MassIVEi | P09455 |
MaxQBi | P09455 |
PaxDbi | P09455 |
PeptideAtlasi | P09455 |
PRIDEi | P09455 |
ProteomicsDBi | 18915 23698 52219 [P09455-1] |
Protocols and materials databases
Antibodypediai | 17981, 484 antibodies from 36 providers |
DNASUi | 5947 |
Genome annotation databases
Ensembli | ENST00000492918; ENSP00000429166; ENSG00000114115 [P09455-3] ENST00000617459; ENSP00000477621; ENSG00000114115 [P09455-2] ENST00000619087; ENSP00000482165; ENSG00000114115 |
GeneIDi | 5947 |
KEGGi | hsa:5947 |
UCSCi | uc011bmx.2, human [P09455-1] |
Organism-specific databases
CTDi | 5947 |
DisGeNETi | 5947 |
GeneCardsi | RBP1 |
HGNCi | HGNC:9919, RBP1 |
HPAi | ENSG00000114115, Tissue enhanced (ovary, pancreas) |
MIMi | 180260, gene |
neXtProti | NX_P09455 |
OpenTargetsi | ENSG00000114115 |
PharmGKBi | PA34286 |
VEuPathDBi | HostDB:ENSG00000114115 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4015, Eukaryota |
GeneTreei | ENSGT00940000159675 |
HOGENOMi | CLU_113772_5_1_1 |
InParanoidi | P09455 |
OrthoDBi | 1377380at2759 |
PhylomeDBi | P09455 |
TreeFami | TF316894 |
Enzyme and pathway databases
PathwayCommonsi | P09455 |
Reactomei | R-HSA-2453864, Retinoid cycle disease events R-HSA-2453902, The canonical retinoid cycle in rods (twilight vision) R-HSA-975634, Retinoid metabolism and transport |
SignaLinki | P09455 |
SIGNORi | P09455 |
Miscellaneous databases
BioGRID-ORCSi | 5947, 5 hits in 1036 CRISPR screens |
ChiTaRSi | RBP1, human |
GeneWikii | RBP1 |
GenomeRNAii | 5947 |
Pharosi | P09455, Tbio |
PROi | PR:P09455 |
RNActi | P09455, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000114115, Expressed in pigmented layer of retina and 222 other tissues |
ExpressionAtlasi | P09455, baseline and differential |
Genevisiblei | P09455, HS |
Family and domain databases
Gene3Di | 2.40.128.20, 1 hit |
InterProi | View protein in InterPro IPR012674, Calycin IPR031264, CRBP1 IPR000463, Fatty_acid-bd IPR031259, ILBP IPR000566, Lipocln_cytosolic_FA-bd_dom |
PANTHERi | PTHR11955, PTHR11955, 1 hit PTHR11955:SF56, PTHR11955:SF56, 1 hit |
Pfami | View protein in Pfam PF00061, Lipocalin, 1 hit |
PRINTSi | PR00178, FATTYACIDBP |
SUPFAMi | SSF50814, SSF50814, 1 hit |
PROSITEi | View protein in PROSITE PS00214, FABP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RET1_HUMAN | |
Accessioni | P09455Primary (citable) accession number: P09455 Secondary accession number(s): A8K2Q0 Q6FGX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families