UniProtKB - P09450 (JUNB_MOUSE)
Transcription factor JunB
Junb
Functioni
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (PubMed:2498083).
1 PublicationGO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cellular response to calcium ion Source: MGI
- decidualization Source: MGI
- embryonic process involved in female pregnancy Source: MGI
- in utero embryonic development Source: MGI
- labyrinthine layer blood vessel development Source: MGI
- osteoblast differentiation Source: MGI
- osteoblast proliferation Source: MGI
- osteoclast differentiation Source: MGI
- positive regulation of cell differentiation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of cell cycle Source: MGI
- regulation of cell population proliferation Source: GO_Central
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- transcription by RNA polymerase II Source: MGI
- trophectodermal cell differentiation Source: MGI
- vasculogenesis Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor JunBCuratedAlternative name(s): MyD21 Transcription factor AP-1 subunit JunBCurated |
Gene namesi | Name:Junb Synonyms:Jun-b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96647, Junb |
VEuPathDBi | HostDB:ENSMUSG00000052837 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
- transcription factor AP-1 complex Source: MGI
Other locations
- transcription regulator complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076439 | 1 – 344 | Transcription factor JunBAdd BLAST | 344 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 4 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 33 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 36 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 81 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 102 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 104 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 117 | PhosphoserineBy similarity | 1 | |
Cross-linki | 138 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 237 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 237 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 237 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 248 | PhosphoserineCombined sources | 1 | |
Modified residuei | 252 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 256 | PhosphoserineCombined sources | 1 | |
Cross-linki | 340 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P09450 |
jPOSTi | P09450 |
PaxDbi | P09450 |
PeptideAtlasi | P09450 |
PRIDEi | P09450 |
ProteomicsDBi | 269036 |
PTM databases
iPTMneti | P09450 |
PhosphoSitePlusi | P09450 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000052837, Expressed in granulocyte and 196 other tissues |
ExpressionAtlasi | P09450, baseline and differential |
Genevisiblei | P09450, MM |
Interactioni
Subunit structurei
Binds DNA as a homodimer or as a heterodimer with another member of the Jun/Fos family (By similarity).
Component of an AP-1 transcription factor complex composed of JUN-FOS heterodimers (PubMed:2498083). As part of the AP-1 transcription factor complex, forms heterodimers with FOSB, thereby binding to the AP-1 consensus sequence and stimulating transcription (PubMed:2498083).
Interacts with NFE2 (via its WW domains) (By similarity).
By similarity1 PublicationBinary interactionsi
P09450
With | #Exp. | IntAct |
---|---|---|
MDV005 [Q9DGW5] from Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987). | 2 | EBI-5347760,EBI-10889526 |
Protein-protein interaction databases
BioGRIDi | 200872, 106 interactors |
CORUMi | P09450 |
DIPi | DIP-1069N |
ELMi | P09450 |
IntActi | P09450, 105 interactors |
STRINGi | 10090.ENSMUSP00000064680 |
Miscellaneous databases
RNActi | P09450, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 265 – 328 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 75 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 181 – 202 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 237 – 257 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 265 – 292 | Basic motifPROSITE-ProRule annotationAdd BLAST | 28 | |
Regioni | 293 – 321 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 29 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0837, Eukaryota |
GeneTreei | ENSGT00940000161195 |
HOGENOMi | CLU_057007_0_0_1 |
InParanoidi | P09450 |
OMAi | PTPAHYL |
OrthoDBi | 1090460at2759 |
PhylomeDBi | P09450 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR046347, bZIP_sf IPR005643, JNK IPR029822, JunB IPR002112, Leuzip_Jun IPR008917, TF_DNA-bd_sf |
PANTHERi | PTHR11462:SF37, PTHR11462:SF37, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit PF03957, Jun, 1 hit |
PRINTSi | PR00043, LEUZIPPRJUN |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MCTKMEQPFY HDDSYAAAGY GRSPGSLSLH DYKLLKPTLA LNLADPYRGL
60 70 80 90 100
KGPGARGPGP EGSGAGSYFS GQGSDTGASL KLASTELERL IVPNSNGVIT
110 120 130 140 150
TTPTPPGQYF YPRGGGSGGG TGGGVTEEQE GFADGFVKAL DDLHKMNHVT
160 170 180 190 200
PPNVSLGASG GPQAGPGGVY AGPEPPPVYT NLSSYSPASA PSGGSGTAVG
210 220 230 240 250
TGSSYPTATI SYLPHAPPFA GGHPAQLGLS RGASAFKEEP QTVPEARSRD
260 270 280 290 300
ATPPVSPINM EDQERIKVER KRLRNRLAAT KCRKRKLERI ARLEDKVKTL
310 320 330 340
KAENAGLSSA AGLLREQVAQ LKQKVMTHVS NGCQLLLGVK GHAF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 53 | P → S in BAC40473 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 200 | G → R in AAA74916 (PubMed:8530030).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03236 mRNA Translation: AAA39343.1 U20735 Genomic DNA Translation: AAA74916.1 AK088643 mRNA Translation: BAC40473.1 BC003790 mRNA Translation: AAH03790.1 X54332 mRNA No translation available. |
CCDSi | CCDS22488.1 |
PIRi | A28963, TVMSJB |
RefSeqi | NP_032442.1, NM_008416.3 |
Genome annotation databases
Ensembli | ENSMUST00000064922; ENSMUSP00000064680; ENSMUSG00000052837 |
GeneIDi | 16477 |
KEGGi | mmu:16477 |
UCSCi | uc009mop.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03236 mRNA Translation: AAA39343.1 U20735 Genomic DNA Translation: AAA74916.1 AK088643 mRNA Translation: BAC40473.1 BC003790 mRNA Translation: AAH03790.1 X54332 mRNA No translation available. |
CCDSi | CCDS22488.1 |
PIRi | A28963, TVMSJB |
RefSeqi | NP_032442.1, NM_008416.3 |
3D structure databases
AlphaFoldDBi | P09450 |
SMRi | P09450 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200872, 106 interactors |
CORUMi | P09450 |
DIPi | DIP-1069N |
ELMi | P09450 |
IntActi | P09450, 105 interactors |
STRINGi | 10090.ENSMUSP00000064680 |
PTM databases
iPTMneti | P09450 |
PhosphoSitePlusi | P09450 |
Proteomic databases
EPDi | P09450 |
jPOSTi | P09450 |
PaxDbi | P09450 |
PeptideAtlasi | P09450 |
PRIDEi | P09450 |
ProteomicsDBi | 269036 |
Protocols and materials databases
Antibodypediai | 3847, 908 antibodies from 44 providers |
DNASUi | 16477 |
Genome annotation databases
Ensembli | ENSMUST00000064922; ENSMUSP00000064680; ENSMUSG00000052837 |
GeneIDi | 16477 |
KEGGi | mmu:16477 |
UCSCi | uc009mop.1, mouse |
Organism-specific databases
CTDi | 3726 |
MGIi | MGI:96647, Junb |
VEuPathDBi | HostDB:ENSMUSG00000052837 |
Phylogenomic databases
eggNOGi | KOG0837, Eukaryota |
GeneTreei | ENSGT00940000161195 |
HOGENOMi | CLU_057007_0_0_1 |
InParanoidi | P09450 |
OMAi | PTPAHYL |
OrthoDBi | 1090460at2759 |
PhylomeDBi | P09450 |
Miscellaneous databases
BioGRID-ORCSi | 16477, 6 hits in 76 CRISPR screens |
ChiTaRSi | Junb, mouse |
PROi | PR:P09450 |
RNActi | P09450, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052837, Expressed in granulocyte and 196 other tissues |
ExpressionAtlasi | P09450, baseline and differential |
Genevisiblei | P09450, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR046347, bZIP_sf IPR005643, JNK IPR029822, JunB IPR002112, Leuzip_Jun IPR008917, TF_DNA-bd_sf |
PANTHERi | PTHR11462:SF37, PTHR11462:SF37, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit PF03957, Jun, 1 hit |
PRINTSi | PR00043, LEUZIPPRJUN |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | JUNB_MOUSE | |
Accessioni | P09450Primary (citable) accession number: P09450 Secondary accession number(s): Q8C2G9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | May 25, 2022 | |
This is version 192 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families