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Protein

Cytochrome b2, mitochondrial

Gene

CYB2

Organism
Wickerhamomyces anomalus (Yeast) (Hansenula anomala)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.

Cofactori

Protein has several cofactor binding sites:
  • FMNPROSITE-ProRule annotation
  • heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi138Iron (heme axial ligand)PROSITE-ProRule annotation1
Binding sitei208Heme bBy similarity1
Binding sitei357Heme bBy similarity1
Active sitei432Proton acceptorPROSITE-ProRule annotation1
Binding sitei435SubstratePROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
LigandFlavoprotein, FMN, Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b2, mitochondrial (EC:1.1.2.3)
Alternative name(s):
L-lactate dehydrogenase [Cytochrome]
L-lactate ferricytochrome C oxidoreductase
Short name:
L-LCR
Gene namesi
Name:CYB2
OrganismiWickerhamomyces anomalus (Yeast) (Hansenula anomala)
Taxonomic identifieri4927 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeWickerhamomyces

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 73MitochondrionAdd BLAST73
ChainiPRO_000000647974 – 573Cytochrome b2, mitochondrialAdd BLAST500

Proteomic databases

PRIDEiP09437

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP09437
SMRiP09437
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 157Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST78
Domaini182 – 542FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST361

Keywords - Domaini

Transit peptide

Family and domain databases

CDDicd02922 FCB2_FMN, 1 hit
Gene3Di3.10.120.10, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR000262 FMN-dep_DH
IPR037396 FMN_HAD
IPR008259 FMN_hydac_DH_AS
IPR037458 L-MDH/L-LDH_FMN-bd
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF01070 FMN_dh, 1 hit
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit
PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09437-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFKSQLRTAT ARSSFRSLAS KLNPQRFNSS KTPLLNATRG SNRSKNSLIA
60 70 80 90 100
LAISLSAVSS SYYLYQKDKF ISADVPHWKD IELTPEIVSQ HNKKDDLWVV
110 120 130 140 150
LNGQVYDLTD FLPNHPGGQK IIIRYAGKDA TKIFVPIHPP DTIEKFIPPE
160 170 180 190 200
KHLGPLVGEF EQEEEELSDE EIDRLERIER KPPLSQMINL HDFETIARQI
210 220 230 240 250
LPPPALAYYC SAADDEVTLR ENHNAYHRIF FNPKILIDVK DVDISTEFFG
260 270 280 290 300
EKTSAPFYIS ATALAKLGHP EGEVAIAKGA GREDVVQMIS TLASCSFDEI
310 320 330 340 350
ADARIPGQQQ WYQLYVNADR SITEKAVRHA EERGMKGLFI TVDAPSLGRR
360 370 380 390 400
EKDMKMKFEA DSDVQGDDED IDRSQGASRA LSSFIDPSLS WKDIAFIKSI
410 420 430 440 450
TKMPIVIKGV QRKEDVLLAA EHGLQGVVLS NHGGRQLDYT RAPVEVLAEV
460 470 480 490 500
MPILKERGLD QKIDIFVDGG VRRGTDVLKA LCLGAKGVGL GRPFLYAMSS
510 520 530 540 550
YGDKGVTKAI QLLKDEIEMN MRLLGVNKIE ELTPELLDTR SIHNRAVPVA
560 570
KDYLYEQNYQ RMSGAEFRPG IED
Length:573
Mass (Da):64,202
Last modified:January 1, 1990 - v2
Checksum:i83EEF645C580BC8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16051 Genomic DNA Translation: CAA34183.1
PIRiS06600

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16051 Genomic DNA Translation: CAA34183.1
PIRiS06600

3D structure databases

ProteinModelPortaliP09437
SMRiP09437
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP09437

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd02922 FCB2_FMN, 1 hit
Gene3Di3.10.120.10, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR000262 FMN-dep_DH
IPR037396 FMN_HAD
IPR008259 FMN_hydac_DH_AS
IPR037458 L-MDH/L-LDH_FMN-bd
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF01070 FMN_dh, 1 hit
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit
PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYB2_WICAO
AccessioniPrimary (citable) accession number: P09437
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 1, 1990
Last modified: January 31, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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