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Protein

Myc proto-oncogene protein

Gene

Myc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes (PubMed:17304222). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-5689880 Ub-specific processing proteases
R-RNO-8866911 TFAP2 (AP-2) family regulates transcription of cell cycle factors
R-RNO-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Myc proto-oncogene protein
Alternative name(s):
Proto-oncogene c-Myc
Transcription factor p64
Gene namesi
Name:Myc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3130 Myc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273001 – 439Myc proto-oncogene proteinAdd BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1
Cross-linki52Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei58Phosphothreonine; by GSK3; alternateBy similarity1
Glycosylationi58O-linked (GlcNAc) threonine; alternateBy similarity1
Modified residuei62Phosphoserine; by DYRK2, GSK3 and CDK2By similarity1
Modified residuei71PhosphoserineBy similarity1
Modified residuei144N6-acetyllysine; by PCAF; alternateBy similarity1
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei149N6-acetyllysine; alternateBy similarity1
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei158N6-acetyllysine; by PCAFBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei275N6-acetyllysine; by PCAFBy similarity1
Modified residuei293PhosphoserineBy similarity1
Cross-linki298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei317N6-acetyllysine; by PCAFBy similarity1
Modified residuei323N6-acetyllysine; by PCAFBy similarity1
Modified residuei329Phosphoserine; by PIM2; in vitroBy similarity1
Modified residuei371N6-acetyllysine; by PCAFBy similarity1

Post-translational modificationi

Phosphorylated by PRKDC. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome (By similarity). Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC. Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence (By similarity).By similarity
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. Ubiquitination is counteracted by USP28 in the nucleoplasm and USP36 in the nucleolus, both interacting with of FBXW7, leading to its deubiquitination and preventing degradation. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP09416
PRIDEiP09416

PTM databases

iPTMnetiP09416
PhosphoSitePlusiP09416

Expressioni

Gene expression databases

ExpressionAtlasiP09416 baseline and differential

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX (By similarity). Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2. Interacts with RIOX1 (By similarity). The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity (PubMed:17304222). Interacts with TRIM6 (By similarity). Interacts with NPM1; the binary complex is recruited to the promoter of MYC target genes and enhances their transcription (By similarity). Interacts with CIP2A; leading to the stabilization of MYC (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246723, 3 interactors
CORUMiP09416
DIPiDIP-28140N
IntActiP09416, 3 interactors
MINTiP09416
STRINGi10116.ENSRNOP00000006188

Structurei

3D structure databases

ProteinModelPortaliP09416
SMRiP09416
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini354 – 406bHLHPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni413 – 434Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 37Poly-Gln4
Compositional biasi89 – 92Poly-Gly4
Compositional biasi218 – 223Poly-Ser6

Phylogenomic databases

eggNOGiENOG410IFSM Eukaryota
ENOG41124Q3 LUCA
GeneTreeiENSGT00510000046414
HOGENOMiHOG000043075
HOVERGENiHBG000472
InParanoidiP09416
KOiK04377
PhylomeDBiP09416

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003327 Myc-LZ
IPR002418 Tscrpt_reg_Myc
IPR012682 Tscrpt_reg_Myc_N
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF02344 Myc-LZ, 1 hit
PF01056 Myc_N, 1 hit
PIRSFiPIRSF001705 Myc_protein, 1 hit
PRINTSiPR00044 LEUZIPPRMYC
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P09416-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLNVSFANR NYDLDYDSVQ PYFICDEEEN FYHQQQQSEL QPPAPSEDIW
60 70 80 90 100
KKFELLPTPP LSPSRRSGLC SPSYVAVATS FSPREDDDGG GGNFSTADQL
110 120 130 140 150
EMMTELLGGD MVNQSFICDP DDETFIKNII IQDCMWSGFS AAAKLVSEKL
160 170 180 190 200
ASYQAARKDS TSLSPARGHS VCSTSSLYLQ DLTAAASECI DPSVVFPYPL
210 220 230 240 250
NDSSSPKSCT SSDSTAFSSS SDSLLSSESS PRATPEPLVL HEETPPTTSS
260 270 280 290 300
DSEEEQDDEE EIDVVSVEKR QPPAKRSESG SSPSRGHSKP PHSPLVLKRC
310 320 330 340 350
HVSTHQHNYA APPSTRKDYP AAKRAKLDSG RVLKQISNNR KCSSPRSSDT
360 370 380 390 400
EENDKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT
410 420 430
AYILSVQADE HKLISEKDLL RKRREQLKHK LEQLRNSGA
Length:439
Mass (Da):48,898
Last modified:July 1, 1989 - v1
Checksum:i7547DCCECF74554F
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WFG7F8WFG7_RAT
Myc proto-oncogene protein
Myc rCG_59927
439Annotation score:
A0A1B0GWT1A0A1B0GWT1_RAT
Myc proto-oncogene protein
Myc
219Annotation score:

Sequence cautioni

The sequence AAH91699 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00396 Genomic DNA Translation: CAA68459.2
AY679729 mRNA Translation: AAT92511.1
AY679730 mRNA Translation: AAT92512.1
BC091699 mRNA Translation: AAH91699.2 Different initiation.
PIRiA26801 TVRTMC
RefSeqiNP_036735.2, NM_012603.2
UniGeneiRn.12072

Genome annotation databases

EnsembliENSRNOT00000006188; ENSRNOP00000006188; ENSRNOG00000004500
GeneIDi24577
KEGGirno:24577
UCSCiRGD:3130 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00396 Genomic DNA Translation: CAA68459.2
AY679729 mRNA Translation: AAT92511.1
AY679730 mRNA Translation: AAT92512.1
BC091699 mRNA Translation: AAH91699.2 Different initiation.
PIRiA26801 TVRTMC
RefSeqiNP_036735.2, NM_012603.2
UniGeneiRn.12072

3D structure databases

ProteinModelPortaliP09416
SMRiP09416
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246723, 3 interactors
CORUMiP09416
DIPiDIP-28140N
IntActiP09416, 3 interactors
MINTiP09416
STRINGi10116.ENSRNOP00000006188

PTM databases

iPTMnetiP09416
PhosphoSitePlusiP09416

Proteomic databases

PaxDbiP09416
PRIDEiP09416

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006188; ENSRNOP00000006188; ENSRNOG00000004500
GeneIDi24577
KEGGirno:24577
UCSCiRGD:3130 rat

Organism-specific databases

CTDi4609
RGDi3130 Myc

Phylogenomic databases

eggNOGiENOG410IFSM Eukaryota
ENOG41124Q3 LUCA
GeneTreeiENSGT00510000046414
HOGENOMiHOG000043075
HOVERGENiHBG000472
InParanoidiP09416
KOiK04377
PhylomeDBiP09416

Enzyme and pathway databases

ReactomeiR-RNO-5689880 Ub-specific processing proteases
R-RNO-8866911 TFAP2 (AP-2) family regulates transcription of cell cycle factors
R-RNO-9018519 Estrogen-dependent gene expression

Miscellaneous databases

PROiPR:P09416

Gene expression databases

ExpressionAtlasiP09416 baseline and differential

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003327 Myc-LZ
IPR002418 Tscrpt_reg_Myc
IPR012682 Tscrpt_reg_Myc_N
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF02344 Myc-LZ, 1 hit
PF01056 Myc_N, 1 hit
PIRSFiPIRSF001705 Myc_protein, 1 hit
PRINTSiPR00044 LEUZIPPRMYC
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMYC_RAT
AccessioniPrimary (citable) accession number: P09416
Secondary accession number(s): Q6B500
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 7, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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