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Protein

Nucleolin

Gene

Ncl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolin
Alternative name(s):
Protein C23
Gene namesi
Name:Ncl
Synonyms:Nuc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97286 Ncl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000816932 – 707NucleolinAdd BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9N6-acetyllysineCombined sources1
Modified residuei15N6-acetyllysineCombined sources1
Modified residuei16N6-acetyllysineCombined sources1
Modified residuei28PhosphoserineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei40PhosphoserineCombined sources1
Modified residuei41PhosphoserineCombined sources1
Modified residuei67PhosphoserineBy similarity1
Modified residuei69PhosphothreonineBy similarity1
Modified residuei76PhosphothreonineBy similarity1
Modified residuei84PhosphothreonineBy similarity1
Modified residuei92PhosphothreonineBy similarity1
Modified residuei96N6-acetyllysineCombined sources1
Modified residuei99PhosphothreonineBy similarity1
Modified residuei102N6-acetyllysineCombined sources1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei109N6-acetyllysineCombined sources1
Modified residuei116N6-acetyllysineCombined sources1
Modified residuei121PhosphothreonineBy similarity1
Modified residuei124N6-acetyllysineBy similarity1
Modified residuei145PhosphoserineCombined sources1
Modified residuei157PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei220PhosphothreonineBy similarity1
Cross-linki299Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki299Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei303PhosphoserineCombined sources1
Modified residuei320N6-acetyllysineBy similarity1
Cross-linki326Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki326Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei350N6-acetyllysineCombined sources1
Modified residuei358PhosphoserineBy similarity1
Modified residuei369PhosphothreonineBy similarity1
Cross-linki372Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei379N6-acetyllysine; alternateBy similarity1
Cross-linki379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei400N6-acetyllysineBy similarity1
Modified residuei403PhosphoserineCombined sources1
Modified residuei407PhosphothreonineBy similarity1
Modified residuei429N6-acetyllysineCombined sources1
Modified residuei446N6-acetyllysineCombined sources1
Modified residuei460PhosphoserineCombined sources1
Modified residuei462PhosphoserineBy similarity1
Modified residuei469N6-acetyllysineCombined sources1
Modified residuei478N6-acetyllysineCombined sources1
Modified residuei514N6-acetyllysine; alternateBy similarity1
Cross-linki514Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei522N6-acetyllysineCombined sources1
Modified residuei569N6-acetyllysineBy similarity1
Modified residuei574N6-acetyllysine; alternateCombined sources1
Cross-linki574Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei577PhosphoserineBy similarity1
Cross-linki586Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki586Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei588PhosphoserineBy similarity1
Modified residuei616PhosphoserineCombined sources1
Cross-linki621Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei643N6-acetyllysineCombined sources1
Modified residuei653Asymmetric dimethylarginineBy similarity1
Modified residuei657Asymmetric dimethylarginineBy similarity1
Modified residuei663Asymmetric dimethylarginineBy similarity1
Modified residuei667Asymmetric dimethylarginineBy similarity1
Modified residuei670Asymmetric dimethylarginineBy similarity1
Modified residuei676Asymmetric dimethylarginineBy similarity1
Modified residuei678Asymmetric dimethylarginineBy similarity1
Modified residuei684Asymmetric dimethylarginineBy similarity1
Modified residuei688Asymmetric dimethylarginineBy similarity1
Modified residuei691Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei691Omega-N-methylarginine; alternateCombined sources1

Post-translational modificationi

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
Symmetrically methylated by PRMT5.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP09405
MaxQBiP09405
PaxDbiP09405
PeptideAtlasiP09405
PRIDEiP09405
TopDownProteomicsiP09405

2D gel databases

REPRODUCTION-2DPAGEiP09405

PTM databases

iPTMnetiP09405
PhosphoSitePlusiP09405
SwissPalmiP09405

Miscellaneous databases

PMAP-CutDBiP09405

Expressioni

Tissue specificityi

Expressed in B-cells that have been induced to switch to various Ig isotypes.1 Publication

Gene expression databases

BgeeiENSMUSG00000026234 Expressed in 292 organ(s), highest expression level in placenta
CleanExiMM_NCL
ExpressionAtlasiP09405 baseline and differential
GenevisibleiP09405 MM

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (By similarity). Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70 (PubMed:9642267). Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin (By similarity). Interacts with AICDA (PubMed:21518874). Interacts with APTX (By similarity). Interacts with C1QBP (By similarity). Interacts with ERBB4 (By similarity). Interacts (via C-terminus) with FMR1 isoform 6 (via N-terminus) (By similarity). Interacts with GZF1; this interaction is important for nucleolar localization of GZF1 (By similarity). Interacts with NSUN2 (By similarity). Interacts with NVL (PubMed:21474449). Interacts (via N-terminus domain) with SETX (By similarity). Interacts (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains) with TERT; the interaction is important for nucleolar localization of TERT (By similarity). Interacts with WDR46 (By similarity). Interacts with ZFP36 (By similarity). Interacts with LRRC34 (PubMed:24991885). Interacts with RRP1B (By similarity). Interacts with HNRNPU; this interaction occurs during mitosis (By similarity). Interacts with RIOK1; RIOK1 recruits NCL to PRMT5 for symmetrically methylation (By similarity). Interacts with ZBTB7B (PubMed:28784777).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201706, 12 interactors
CORUMiP09405
IntActiP09405, 17 interactors
MINTiP09405
STRINGi10090.ENSMUSP00000027438

Structurei

3D structure databases

ProteinModelPortaliP09405
SMRiP09405
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati58 – 6518
Repeati75 – 8228
Repeati83 – 9038
Repeati91 – 9848
Repeati99 – 1045; truncated6
Repeati105 – 11268
Repeati120 – 12778
Repeati128 – 13588
Domaini309 – 385RRM 1PROSITE-ProRule annotationAdd BLAST77
Domaini395 – 468RRM 2PROSITE-ProRule annotationAdd BLAST74
Domaini487 – 561RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini569 – 644RRM 4PROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 1358 X 8 AA tandem repeats of X-T-P-X-K-K-X-XAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi143 – 170Asp/Glu-rich (acidic)Add BLAST28
Compositional biasi190 – 215Asp/Glu-rich (acidic)Add BLAST26
Compositional biasi241 – 273Asp/Glu-rich (acidic)Add BLAST33
Compositional biasi646 – 697Arg/Gly/Phe-richAdd BLAST52

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IRS7 Eukaryota
ENOG410XSFV LUCA
GeneTreeiENSGT00900000141052
HOGENOMiHOG000113885
HOVERGENiHBG002295
InParanoidiP09405
KOiK11294
OMAiDMESDEP
OrthoDBiEOG091G15MK
PhylomeDBiP09405
TreeFamiTF328499

Family and domain databases

CDDicd12403 RRM1_NCL, 1 hit
cd12404 RRM2_NCL, 1 hit
cd12405 RRM3_NCL, 1 hit
cd12406 RRM4_NCL, 1 hit
Gene3Di3.30.70.330, 4 hits
InterProiView protein in InterPro
IPR034230 Nucleolin_RRM1
IPR034233 Nucleolin_RRM2
IPR034234 Nucleolin_RRM3
IPR034235 Nucleolin_RRM4
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 4 hits
SMARTiView protein in SMART
SM00360 RRM, 4 hits
SUPFAMiSSF54928 SSF54928, 4 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P09405-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKLAKAGKT HGEAKKMAPP PKEVEEDSED EEMSEDEDDS SGEEEVVIPQ
60 70 80 90 100
KKGKKATTTP AKKVVVSQTK KAAVPTPAKK AAVTPGKKAV ATPAKKNITP
110 120 130 140 150
AKVIPTPGKK GAAQAKALVP TPGKKGAATP AKGAKNGKNA KKEDSDEDED
160 170 180 190 200
EEDEDDSDED EDDEEEDEFE PPIVKGVKPA KAAPAAPASE DEEDDEDEDD
210 220 230 240 250
EEDDDEEEED DSEEEVMEIT TAKGKKTPAK VVPMKAKSVA EEEDDEEEDE
260 270 280 290 300
DDEDEDDEEE DDEDDDEEEE EEEPVKAAPG KRKKEMTKQK EAPEAKKQKV
310 320 330 340 350
EGSEPTTPFN LFIGNLNPNK SVNELKFAIS ELFAKNDLAV VDVRTGTNRK
360 370 380 390 400
FGYVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK
410 420 430 440 450
NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE
460 470 480 490 500
KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK
510 520 530 540 550
ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE
560 570 580 590 600
GRTIRLELQG SNSRSQPSKT LFVKGLSEDT TEETLKESFE GSVRARIVTD
610 620 630 640 650
RETGSSKGFG FVDFNSEEDA KAAKEAMEDG EIDGNKVTLD WAKPKGEGGF
660 670 680 690 700
GGRGGGRGGF GGRGGGRGGR GGFGGRGRGG FGGRGGFRGG RGGGGDFKPQ

GKKTKFE
Length:707
Mass (Da):76,723
Last modified:January 23, 2007 - v2
Checksum:iEE2CE2ACDBF54CD4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WRM5A0A087WRM5_MOUSE
Nucleolin
Ncl
76Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155D → E in AAH05460 (PubMed:15489334).Curated1
Sequence conflicti203 – 204DD → EE in AAH05460 (PubMed:15489334).Curated2
Sequence conflicti245D → E in AAH05460 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07699 Genomic DNA Translation: CAA30538.1
AF318184 mRNA Translation: AAK07920.1
AK050958 mRNA Translation: BAC34476.1
AK083307 mRNA Translation: BAC38858.1
AK144894 mRNA Translation: BAE26119.1
AK161706 mRNA Translation: BAE36542.1
AK163275 mRNA Translation: BAE37270.1
BC005460 mRNA Translation: AAH05460.1
M22089 Genomic DNA Translation: AAA39841.1
CCDSiCCDS35646.1
PIRiA29958 DNMS
RefSeqiNP_035010.3, NM_010880.3
UniGeneiMm.154378
Mm.474153

Genome annotation databases

EnsembliENSMUST00000027438; ENSMUSP00000027438; ENSMUSG00000026234
GeneIDi17975
KEGGimmu:17975
UCSCiuc007bvl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07699 Genomic DNA Translation: CAA30538.1
AF318184 mRNA Translation: AAK07920.1
AK050958 mRNA Translation: BAC34476.1
AK083307 mRNA Translation: BAC38858.1
AK144894 mRNA Translation: BAE26119.1
AK161706 mRNA Translation: BAE36542.1
AK163275 mRNA Translation: BAE37270.1
BC005460 mRNA Translation: AAH05460.1
M22089 Genomic DNA Translation: AAA39841.1
CCDSiCCDS35646.1
PIRiA29958 DNMS
RefSeqiNP_035010.3, NM_010880.3
UniGeneiMm.154378
Mm.474153

3D structure databases

ProteinModelPortaliP09405
SMRiP09405
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201706, 12 interactors
CORUMiP09405
IntActiP09405, 17 interactors
MINTiP09405
STRINGi10090.ENSMUSP00000027438

PTM databases

iPTMnetiP09405
PhosphoSitePlusiP09405
SwissPalmiP09405

2D gel databases

REPRODUCTION-2DPAGEiP09405

Proteomic databases

EPDiP09405
MaxQBiP09405
PaxDbiP09405
PeptideAtlasiP09405
PRIDEiP09405
TopDownProteomicsiP09405

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027438; ENSMUSP00000027438; ENSMUSG00000026234
GeneIDi17975
KEGGimmu:17975
UCSCiuc007bvl.1 mouse

Organism-specific databases

CTDi4691
MGIiMGI:97286 Ncl

Phylogenomic databases

eggNOGiENOG410IRS7 Eukaryota
ENOG410XSFV LUCA
GeneTreeiENSGT00900000141052
HOGENOMiHOG000113885
HOVERGENiHBG002295
InParanoidiP09405
KOiK11294
OMAiDMESDEP
OrthoDBiEOG091G15MK
PhylomeDBiP09405
TreeFamiTF328499

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRSiNcl mouse
PMAP-CutDBiP09405
PROiPR:P09405
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026234 Expressed in 292 organ(s), highest expression level in placenta
CleanExiMM_NCL
ExpressionAtlasiP09405 baseline and differential
GenevisibleiP09405 MM

Family and domain databases

CDDicd12403 RRM1_NCL, 1 hit
cd12404 RRM2_NCL, 1 hit
cd12405 RRM3_NCL, 1 hit
cd12406 RRM4_NCL, 1 hit
Gene3Di3.30.70.330, 4 hits
InterProiView protein in InterPro
IPR034230 Nucleolin_RRM1
IPR034233 Nucleolin_RRM2
IPR034234 Nucleolin_RRM3
IPR034235 Nucleolin_RRM4
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 4 hits
SMARTiView protein in SMART
SM00360 RRM, 4 hits
SUPFAMiSSF54928 SSF54928, 4 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNUCL_MOUSE
AccessioniPrimary (citable) accession number: P09405
Secondary accession number(s): Q548M9
, Q61991, Q8BQD8, Q99K50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 192 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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