UniProtKB - P09394 (GLPQ_ECOLI)
Protein
Glycerophosphodiester phosphodiesterase, periplasmic
Gene
glpQ
Organism
Escherichia coli (strain K12)
Status
Functioni
Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols.
Miscellaneous
There are 2 isozymes of glycerophosphoryl diester phosphodiesterase in E.coli: a periplasmic isozyme (GlpQ) and a cytosolic isozyme (UgpQ).
Catalytic activityi
- EC:3.1.4.461 Publication
Cofactori
Ca2+1 PublicationNote: Binds 1 Ca2+ ion per subunit.1 Publication
Kineticsi
- KM=0.28 mM for glycerophosphocholine1 Publication
- KM=0.24 mM for glycerophosphoethanolamine1 Publication
- KM=0.35 mM for glycerophosphoglycerol1 Publication
- KM=0.59 mM for glycerophosphoserine1 Publication
- KM=1.0 mM for glycerophosphoinositol1 Publication
pH dependencei
Optimum pH is 7.8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 63 | Calcium | 1 | |
Metal bindingi | 65 | Calcium | 1 | |
Metal bindingi | 171 | Calcium | 1 |
GO - Molecular functioni
- calcium ion binding Source: EcoCyc
- glycerophosphodiester phosphodiesterase activity Source: EcoCyc
- metal ion binding Source: EcoCyc
GO - Biological processi
- glycerol metabolic process Source: UniProtKB-KW
- lipid metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Biological process | Glycerol metabolism |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYCPDIESTER-PERI-MONOMER MetaCyc:GLYCPDIESTER-PERI-MONOMER |
BRENDAi | 3.1.4.46, 2026 |
SABIO-RKi | P09394 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycerophosphodiester phosphodiesterase, periplasmic (EC:3.1.4.46)Short name: Glycerophosphoryl diester phosphodiesterase, periplasmic |
Gene namesi | Name:glpQ Ordered Locus Names:b2239, JW2233 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm 1 Publication
Other locations
- periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | 2 PublicationsAdd BLAST | 25 | |
ChainiPRO_0000012594 | 26 – 358 | Glycerophosphodiester phosphodiesterase, periplasmicAdd BLAST | 333 |
Proteomic databases
jPOSTi | P09394 |
PaxDbi | P09394 |
PRIDEi | P09394 |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 4260486, 185 interactors |
IntActi | P09394, 11 interactors |
STRINGi | 511145.b2239 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09394 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09394 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 355 | GP-PDEAdd BLAST | 325 |
Sequence similaritiesi
Belongs to the glycerophosphoryl diester phosphodiesterase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0584, Bacteria |
HOGENOMi | CLU_030226_1_0_6 |
InParanoidi | P09394 |
PhylomeDBi | P09394 |
Family and domain databases
Gene3Di | 3.20.20.190, 1 hit |
InterProi | View protein in InterPro IPR030395, GP_PDE_dom IPR017946, PLC-like_Pdiesterase_TIM-brl |
Pfami | View protein in Pfam PF03009, GDPD, 1 hit |
SUPFAMi | SSF51695, SSF51695, 1 hit |
PROSITEi | View protein in PROSITE PS51704, GP_PDE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P09394-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLTLKNLSM AIMMSTIVMG SSAMAADSNE KIVIAHRGAS GYLPEHTLPA
60 70 80 90 100
KAMAYAQGAD YLEQDLVMTK DDNLVVLHDH YLDRVTDVAD RFPDRARKDG
110 120 130 140 150
RYYAIDFTLD EIKSLKFTEG FDIENGKKVQ TYPGRFPMGK SDFRVHTFEE
160 170 180 190 200
EIEFVQGLNH STGKNIGIYP EIKAPWFHHQ EGKDIAAKTL EVLKKYGYTG
210 220 230 240 250
KDDKVYLQCF DADELKRIKN ELEPKMGMEL NLVQLIAYTD WNETQQKQPD
260 270 280 290 300
GSWVNYNYDW MFKPGAMKQV AEYADGIGPD YHMLIEETSQ PGNIKLTGMV
310 320 330 340 350
QDAQQNKLVV HPYTVRSDKL PEYTPDVNQL YDALYNKAGV NGLFTDFPDK
AVKFLNKE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 27 | D → I AA sequence (PubMed:8899705).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56907 Genomic DNA Translation: CAA40223.1 U00096 Genomic DNA Translation: AAC75299.1 AP009048 Genomic DNA Translation: BAA16058.1 Y00536 Genomic DNA Translation: CAA68599.1 |
PIRi | S15945 |
RefSeqi | NP_416742.1, NC_000913.3 WP_000779105.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75299; AAC75299; b2239 BAA16058; BAA16058; BAA16058 |
GeneIDi | 946725 |
KEGGi | ecj:JW2233 eco:b2239 |
PATRICi | fig|511145.12.peg.2328 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56907 Genomic DNA Translation: CAA40223.1 U00096 Genomic DNA Translation: AAC75299.1 AP009048 Genomic DNA Translation: BAA16058.1 Y00536 Genomic DNA Translation: CAA68599.1 |
PIRi | S15945 |
RefSeqi | NP_416742.1, NC_000913.3 WP_000779105.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T8Q | X-ray | 2.00 | A/B/C/D | 25-358 | [»] | |
1YDY | X-ray | 1.70 | A/B | 1-356 | [»] | |
SMRi | P09394 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260486, 185 interactors |
IntActi | P09394, 11 interactors |
STRINGi | 511145.b2239 |
Proteomic databases
jPOSTi | P09394 |
PaxDbi | P09394 |
PRIDEi | P09394 |
Protocols and materials databases
DNASUi | 946725 |
Genome annotation databases
EnsemblBacteriai | AAC75299; AAC75299; b2239 BAA16058; BAA16058; BAA16058 |
GeneIDi | 946725 |
KEGGi | ecj:JW2233 eco:b2239 |
PATRICi | fig|511145.12.peg.2328 |
Organism-specific databases
EchoBASEi | EB0394 |
Phylogenomic databases
eggNOGi | COG0584, Bacteria |
HOGENOMi | CLU_030226_1_0_6 |
InParanoidi | P09394 |
PhylomeDBi | P09394 |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYCPDIESTER-PERI-MONOMER MetaCyc:GLYCPDIESTER-PERI-MONOMER |
BRENDAi | 3.1.4.46, 2026 |
SABIO-RKi | P09394 |
Miscellaneous databases
EvolutionaryTracei | P09394 |
PROi | PR:P09394 |
Family and domain databases
Gene3Di | 3.20.20.190, 1 hit |
InterProi | View protein in InterPro IPR030395, GP_PDE_dom IPR017946, PLC-like_Pdiesterase_TIM-brl |
Pfami | View protein in Pfam PF03009, GDPD, 1 hit |
SUPFAMi | SSF51695, SSF51695, 1 hit |
PROSITEi | View protein in PROSITE PS51704, GP_PDE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLPQ_ECOLI | |
Accessioni | P09394Primary (citable) accession number: P09394 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | November 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families