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UniProtKB - P09391 (GLPG_ECOLI)
Protein
Rhomboid protease GlpG
Gene
glpG
Organism
Escherichia coli (strain K12)
Status
Functioni
Rhomboid-type serine protease that catalyzes intramembrane proteolysis.
2 PublicationsCaution
Was originally identified as a repressor of the glycerol-3-phosphate regulon.1 Publication
Catalytic activityi
- Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains. EC:3.4.21.105
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 201 | Nucleophile4 Publications | 1 | |
Active sitei | 254 | 4 Publications | 1 |
GO - Molecular functioni
- endopeptidase activity Source: EcoCyc
- identical protein binding Source: IntAct
- serine-type endopeptidase activity Source: EcoCyc
GO - Biological processi
- proteolysis Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10397-MONOMER |
BRENDAi | 3.4.21.105, 2026 |
Protein family/group databases
MEROPSi | S54.016 |
TCDBi | 9.B.104.1.1, the rhomboid protease (rhomboid) family |
Names & Taxonomyi
Protein namesi | Recommended name: Rhomboid protease GlpG (EC:3.4.21.105)Alternative name(s): Intramembrane serine protease |
Gene namesi | Name:glpG Ordered Locus Names:b3424, JW5687 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 93 | CytoplasmicCuratedAdd BLAST | 93 | |
Transmembranei | 94 – 114 | Helical; Name=1CuratedAdd BLAST | 21 | |
Topological domaini | 115 – 141 | PeriplasmicCuratedAdd BLAST | 27 | |
Transmembranei | 142 – 162 | Helical; Name=2CuratedAdd BLAST | 21 | |
Topological domaini | 163 – 168 | CytoplasmicCurated | 6 | |
Transmembranei | 169 – 189 | Helical; Name=3CuratedAdd BLAST | 21 | |
Topological domaini | 190 – 191 | PeriplasmicCurated | 2 | |
Transmembranei | 192 – 212 | Helical; Name=4CuratedAdd BLAST | 21 | |
Topological domaini | 213 – 222 | CytoplasmicCurated | 10 | |
Transmembranei | 223 – 245 | Helical; Name=5CuratedAdd BLAST | 23 | |
Topological domaini | 246 – 249 | PeriplasmicCurated | 4 | |
Transmembranei | 250 – 272 | Helical; Name=6CuratedAdd BLAST | 23 | |
Topological domaini | 273 – 276 | CytoplasmicCurated | 4 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 154 | N → A: Reduced catalytic activity. 2 Publications | 1 | |
Mutagenesisi | 199 | G → C: Loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 201 | S → A or C: Loss of catalytic activity. 4 Publications | 1 | |
Mutagenesisi | 254 | H → A or C: Loss of catalytic activity. 3 Publications | 1 |
Chemistry databases
ChEMBLi | CHEMBL4296282 |
DrugBanki | DB02451, B-nonylglucoside DB04147, Dodecyldimethylamine N-oxide |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087515 | 1 – 276 | Rhomboid protease GlpGAdd BLAST | 276 |
Proteomic databases
PaxDbi | P09391 |
PRIDEi | P09391 |
Interactioni
Binary interactionsi
P09391
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-9134140,EBI-9134140 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262105, 40 interactors 852245, 1 interactor |
DIPi | DIP-9796N |
IntActi | P09391, 3 interactors |
MINTi | P09391 |
STRINGi | 511145.b3424 |
Chemistry databases
BindingDBi | P09391 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09391 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09391 |
Family & Domainsi
Domaini
The loop between transmembrane domains 5 and 6 is flexible and may readily open to the extracellular side to allow water entry into the active site cavity.1 Publication
Sequence similaritiesi
Belongs to the peptidase S54 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0705, Bacteria |
HOGENOMi | CLU_058989_0_0_6 |
InParanoidi | P09391 |
PhylomeDBi | P09391 |
Family and domain databases
Gene3Di | 1.20.1540.10, 1 hit 3.30.70.2350, 1 hit |
HAMAPi | MF_01594, Rhomboid_GlpG, 1 hit |
InterProi | View protein in InterPro IPR038236, GlpG_N_sf IPR022732, Peptidase_S54_GlpG_N IPR022764, Peptidase_S54_rhomboid_dom IPR035952, Rhomboid-like_sf IPR023662, Rhomboid_protease_GlpG |
Pfami | View protein in Pfam PF01694, Rhomboid, 1 hit PF12122, Rhomboid_N, 1 hit |
SUPFAMi | SSF144091, SSF144091, 1 hit |
TIGRFAMsi | TIGR04239, rhombo_GlpG, 1 hit |
i Sequence
Sequence statusi: Complete.
P09391-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLMITSFANP RVAQAFVDYM ATQGVILTIQ QHNQSDVWLA DESQAERVRA
60 70 80 90 100
ELARFLENPA DPRYLAASWQ AGHTGSGLHY RRYPFFAALR ERAGPVTWVM
110 120 130 140 150
MIACVVVFIA MQILGDQEVM LWLAWPFDPT LKFEFWRYFT HALMHFSLMH
160 170 180 190 200
ILFNLLWWWY LGGAVEKRLG SGKLIVITLI SALLSGYVQQ KFSGPWFGGL
210 220 230 240 250
SGVVYALMGY VWLRGERDPQ SGIYLQRGLI IFALIWIVAG WFDLFGMSMA
260 270
NGAHIAGLAV GLAMAFVDSL NARKRK
Sequence cautioni
The sequence AAA58222 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 – 52 | EL → DV in AAA23890 (PubMed:3045764).Curated | 2 | |
Sequence conflicti | 51 – 52 | EL → DV in CAA30398 (PubMed:3045764).Curated | 2 | |
Sequence conflicti | 51 – 52 | EL → DV in AAC28166 (PubMed:8955387).Curated | 2 | |
Sequence conflicti | 51 – 52 | EL → DV in AAA58222 (PubMed:9278503).Curated | 2 | |
Sequence conflicti | 178 – 179 | TL → RS in AAA23890 (PubMed:3045764).Curated | 2 | |
Sequence conflicti | 178 – 179 | TL → RS in CAA30398 (PubMed:3045764).Curated | 2 | |
Sequence conflicti | 193 | S → T in AAA23890 (PubMed:3045764).Curated | 1 | |
Sequence conflicti | 193 | S → T in CAA30398 (PubMed:3045764).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M54940 Genomic DNA Translation: AAA23890.1 X07520 Genomic DNA Translation: CAA30398.1 M96795 Genomic DNA Translation: AAC28166.1 U18997 Genomic DNA Translation: AAA58222.1 Different initiation. U00096 Genomic DNA Translation: AAT48182.1 AP009048 Genomic DNA Translation: BAE77868.1 |
PIRi | C65138, BVECGG |
RefSeqi | WP_000928723.1, NZ_LN832404.1 YP_026220.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48182; AAT48182; b3424 BAE77868; BAE77868; BAE77868 |
GeneIDi | 58460244 947936 |
KEGGi | ecj:JW5687 eco:b3424 |
PATRICi | fig|1411691.4.peg.3305 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M54940 Genomic DNA Translation: AAA23890.1 X07520 Genomic DNA Translation: CAA30398.1 M96795 Genomic DNA Translation: AAC28166.1 U18997 Genomic DNA Translation: AAA58222.1 Different initiation. U00096 Genomic DNA Translation: AAT48182.1 AP009048 Genomic DNA Translation: BAE77868.1 |
PIRi | C65138, BVECGG |
RefSeqi | WP_000928723.1, NZ_LN832404.1 YP_026220.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IC8 | X-ray | 2.10 | A | 91-272 | [»] | |
2IRV | X-ray | 2.30 | A/B | 92-273 | [»] | |
2LEP | NMR | - | A | 1-61 | [»] | |
2NRF | X-ray | 2.60 | A/B | 91-272 | [»] | |
2O7L | X-ray | 2.50 | A | 93-272 | [»] | |
2XOV | X-ray | 1.65 | A | 91-271 | [»] | |
2XOW | X-ray | 2.09 | A | 92-270 | [»] | |
2XTU | X-ray | 1.85 | A | 91-271 | [»] | |
2XTV | X-ray | 1.70 | A | 93-272 | [»] | |
3B44 | X-ray | 1.70 | A | 91-270 | [»] | |
3B45 | X-ray | 1.90 | A | 91-270 | [»] | |
3TXT | X-ray | 2.30 | A | 92-270 | [»] | |
3UBB | X-ray | 2.60 | A | 91-272 | [»] | |
3ZEB | X-ray | 2.20 | A | 92-270 | [»] | |
3ZMH | X-ray | 2.30 | A | 91-270 | [»] | |
3ZMI | X-ray | 2.20 | A | 92-270 | [»] | |
3ZMJ | X-ray | 2.30 | A | 92-270 | [»] | |
3ZOT | X-ray | 2.40 | A | 92-271 | [»] | |
4H1D | X-ray | 2.90 | A | 92-270 | [»] | |
4HDD | X-ray | 1.35 | A | 2-74 | [»] | |
4NJN | X-ray | 2.40 | A | 87-276 | [»] | |
4NJP | X-ray | 2.40 | A | 87-276 | [»] | |
5F5B | X-ray | 2.30 | A | 87-276 | [»] | |
5F5D | X-ray | 2.50 | A | 87-276 | [»] | |
5F5G | X-ray | 2.30 | A | 87-276 | [»] | |
5F5J | X-ray | 2.40 | A | 87-276 | [»] | |
5F5K | X-ray | 2.40 | A | 87-276 | [»] | |
5MT6 | X-ray | 2.16 | A | 91-270 | [»] | |
5MT7 | X-ray | 2.05 | A | 91-271 | [»] | |
5MT8 | X-ray | 1.95 | A | 92-270 | [»] | |
6PJ4 | X-ray | 2.30 | A | 87-276 | [»] | |
6PJ5 | X-ray | 2.40 | A | 87-276 | [»] | |
6PJ7 | X-ray | 2.30 | A | 87-276 | [»] | |
6PJ8 | X-ray | 2.40 | A | 87-276 | [»] | |
6PJ9 | X-ray | 2.50 | A | 87-276 | [»] | |
6PJA | X-ray | 2.60 | A | 87-276 | [»] | |
6PJP | X-ray | 2.45 | A | 87-276 | [»] | |
6PJQ | X-ray | 2.50 | A | 87-276 | [»] | |
6PJR | X-ray | 2.40 | A | 87-276 | [»] | |
6PJU | X-ray | 2.50 | A | 87-276 | [»] | |
6VJ8 | X-ray | 2.30 | A | 87-276 | [»] | |
6VJ9 | X-ray | 2.30 | A | 87-276 | [»] | |
6XRO | X-ray | 2.30 | A | 87-276 | [»] | |
6XRP | X-ray | 2.40 | A | 87-276 | [»] | |
SMRi | P09391 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262105, 40 interactors 852245, 1 interactor |
DIPi | DIP-9796N |
IntActi | P09391, 3 interactors |
MINTi | P09391 |
STRINGi | 511145.b3424 |
Chemistry databases
BindingDBi | P09391 |
ChEMBLi | CHEMBL4296282 |
DrugBanki | DB02451, B-nonylglucoside DB04147, Dodecyldimethylamine N-oxide |
Protein family/group databases
MEROPSi | S54.016 |
TCDBi | 9.B.104.1.1, the rhomboid protease (rhomboid) family |
Proteomic databases
PaxDbi | P09391 |
PRIDEi | P09391 |
Genome annotation databases
EnsemblBacteriai | AAT48182; AAT48182; b3424 BAE77868; BAE77868; BAE77868 |
GeneIDi | 58460244 947936 |
KEGGi | ecj:JW5687 eco:b3424 |
PATRICi | fig|1411691.4.peg.3305 |
Organism-specific databases
EchoBASEi | EB0392 |
Phylogenomic databases
eggNOGi | COG0705, Bacteria |
HOGENOMi | CLU_058989_0_0_6 |
InParanoidi | P09391 |
PhylomeDBi | P09391 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10397-MONOMER |
BRENDAi | 3.4.21.105, 2026 |
Miscellaneous databases
EvolutionaryTracei | P09391 |
PROi | PR:P09391 |
Family and domain databases
Gene3Di | 1.20.1540.10, 1 hit 3.30.70.2350, 1 hit |
HAMAPi | MF_01594, Rhomboid_GlpG, 1 hit |
InterProi | View protein in InterPro IPR038236, GlpG_N_sf IPR022732, Peptidase_S54_GlpG_N IPR022764, Peptidase_S54_rhomboid_dom IPR035952, Rhomboid-like_sf IPR023662, Rhomboid_protease_GlpG |
Pfami | View protein in Pfam PF01694, Rhomboid, 1 hit PF12122, Rhomboid_N, 1 hit |
SUPFAMi | SSF144091, SSF144091, 1 hit |
TIGRFAMsi | TIGR04239, rhombo_GlpG, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLPG_ECOLI | |
Accessioni | P09391Primary (citable) accession number: P09391 Secondary accession number(s): P76691, Q2M788, Q6BF32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 23, 2022 | |
This is version 171 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families