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Protein

Shiga-like toxin 2 subunit A

Gene

stxA2

Organism
Enterobacteria phage 933W (Bacteriophage 933W)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein synthesis inhibitor, Toxin, Viral exotoxin
Biological processModulation of host virulence by virus, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga-like toxin 2 subunit A (EC:3.2.2.22)
Short name:
SLT-2 A subunit
Short name:
SLT-2a
Short name:
SLT-IIa
Alternative name(s):
Verocytotoxin 2 subunit A
Verotoxin 2 subunit A
rRNA N-glycosidase 2
Gene namesi
Name:stxA2
Synonyms:stx2A
Ordered Locus Names:L0103
OrganismiEnterobacteria phage 933W (Bacteriophage 933W)
Taxonomic identifieri10730 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeSepvirinaeNona33virus
Virus hostiEscherichia coli O157:H7 [TaxID: 83334]
Proteomesi
  • UP000002135 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003079223 – 319Shiga-like toxin 2 subunit AAdd BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi263 ↔ 282

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei272 – 273Cleavage; by furinBy similarity2

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Shiga-like toxin contains a single A subunit and multiple copies of a B subunit.

Protein-protein interaction databases

IntActiP09385, 1 interactor

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP09385
SMRiP09385
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09385

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 272A1By similarityAdd BLAST250
Regioni273 – 314A2By similarityAdd BLAST42

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK11006
OrthoDBiVOG090002CW

Family and domain databases

Gene3Di3.40.420.10, 1 hit
4.10.470.10, 1 hit
InterProiView protein in InterPro
IPR036041 Ribosome-inact_prot_sf
IPR001574 Ribosome_inactivat_prot
IPR017988 Ribosome_inactivat_prot_CS
IPR016138 Ribosome_inactivat_prot_sub1
IPR016139 Ribosome_inactivat_prot_sub2
IPR016331 Shiga-like_toxin_subunit_A
PfamiView protein in Pfam
PF00161 RIP, 1 hit
PIRSFiPIRSF001924 Shigella_toxin_subunit_A, 1 hit
SUPFAMiSSF56371 SSF56371, 1 hit
PROSITEiView protein in PROSITE
PS00275 SHIGA_RICIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09385-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKCILFKWVL CLLLGFSSVS YSREFTIDFS TQQSYVSSLN SIRTEISTPL
60 70 80 90 100
EHISQGTTSV SVINHTPPGS YFAVDIRGLD VYQARFDHLR LIIEQNNLYV
110 120 130 140 150
AGFVNTATNT FYRFSDFTHI SVPGVTTVSM TTDSSYTTLQ RVAALERSGM
160 170 180 190 200
QISRHSLVSS YLALMEFSGN TMTRDASRAV LRFVTVTAEA LRFRQIQREF
210 220 230 240 250
RQALSETAPV YTMTPGDVDL TLNWGRISNV LPEYRGEDGV RVGRISFNNI
260 270 280 290 300
SAILGTVAVI LNCHHQGARS VRAVNEESQP ECQITGDRPV IKINNTLWES
310
NTAAAFLNRK SQFLYTTGK
Length:319
Mass (Da):35,714
Last modified:February 1, 1996 - v2
Checksum:i98F73319ACAE48D6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17S → P in strain: OX3:H21. 1
Natural varianti26T → M in strain: OX3:H21. 1
Natural varianti277E → D in strain: FLY16 and CS1718. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA Translation: CAA30714.1
M59432 Unassigned DNA Translation: AAA19623.1
L11079 Unassigned DNA Translation: AAA16362.1
Y10775 Genomic DNA Translation: CAA71747.1
AB017524 Genomic DNA Translation: BAA33759.1
AB015057 Genomic DNA Translation: BAA34372.1
AF461167 Genomic DNA Translation: AAM70033.1
AB048239 Genomic DNA Translation: BAB83026.1
AB048240 Genomic DNA Translation: BAB83028.1
AY443052 Genomic DNA Translation: AAS07596.1
AF125520 Genomic DNA Translation: AAD25445.1
PIRiI76713
S01032
RefSeqiNP_049500.1, NC_000924.1

Genome annotation databases

GeneIDi1261950
KEGGivg:1261950

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA Translation: CAA30714.1
M59432 Unassigned DNA Translation: AAA19623.1
L11079 Unassigned DNA Translation: AAA16362.1
Y10775 Genomic DNA Translation: CAA71747.1
AB017524 Genomic DNA Translation: BAA33759.1
AB015057 Genomic DNA Translation: BAA34372.1
AF461167 Genomic DNA Translation: AAM70033.1
AB048239 Genomic DNA Translation: BAB83026.1
AB048240 Genomic DNA Translation: BAB83028.1
AY443052 Genomic DNA Translation: AAS07596.1
AF125520 Genomic DNA Translation: AAD25445.1
PIRiI76713
S01032
RefSeqiNP_049500.1, NC_000924.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77A23-319[»]
2GA4X-ray1.80A23-319[»]
ProteinModelPortaliP09385
SMRiP09385
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09385, 1 interactor

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261950
KEGGivg:1261950

Phylogenomic databases

KOiK11006
OrthoDBiVOG090002CW

Miscellaneous databases

EvolutionaryTraceiP09385

Family and domain databases

Gene3Di3.40.420.10, 1 hit
4.10.470.10, 1 hit
InterProiView protein in InterPro
IPR036041 Ribosome-inact_prot_sf
IPR001574 Ribosome_inactivat_prot
IPR017988 Ribosome_inactivat_prot_CS
IPR016138 Ribosome_inactivat_prot_sub1
IPR016139 Ribosome_inactivat_prot_sub2
IPR016331 Shiga-like_toxin_subunit_A
PfamiView protein in Pfam
PF00161 RIP, 1 hit
PIRSFiPIRSF001924 Shigella_toxin_subunit_A, 1 hit
SUPFAMiSSF56371 SSF56371, 1 hit
PROSITEiView protein in PROSITE
PS00275 SHIGA_RICIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTXA_BP933
AccessioniPrimary (citable) accession number: P09385
Secondary accession number(s): Q9R398
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: November 22, 2017
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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