The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P09385 (STXA_BP933)
Protein
Shiga-like toxin 2 subunit A
Gene
stxA2
Organism
Escherichia phage 933W (Bacteriophage 933W)
Status
Functioni
The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.
Catalytic activityi
- Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. EC:3.2.2.22
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 189 | By similarity | 1 |
GO - Molecular functioni
- rRNA N-glycosylase activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- modulation of host virulence by virus Source: UniProtKB-KW
- negative regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protein synthesis inhibitor, Toxin, Viral exotoxin |
Biological process | Modulation of host virulence by virus, Virulence |
Names & Taxonomyi
Protein namesi | Recommended name: Shiga-like toxin 2 subunit A (EC:3.2.2.22)Short name: SLT-2 A subunit Short name: SLT-2a Short name: SLT-IIa Alternative name(s): Verocytotoxin 2 subunit A Verotoxin 2 subunit A rRNA N-glycosidase 2 |
Gene namesi | Name:stxA2 Synonyms:stx2A Ordered Locus Names:L0103 |
Organismi | Escherichia phage 933W (Bacteriophage 933W) |
Taxonomic identifieri | 10730 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Podoviridae › Sepvirinae › Traversvirus › |
Virus hosti | Escherichia coli O157:H7 [TaxID: 83334] |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000030792 | 23 – 319 | Shiga-like toxin 2 subunit AAdd BLAST | 297 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 263 ↔ 282 |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 272 – 273 | Cleavage; by furinBy similarity | 2 |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Shiga-like toxin contains a single A subunit and multiple copies of a B subunit.
Protein-protein interaction databases
IntActi | P09385, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09385 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09385 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 23 – 272 | A1By similarityAdd BLAST | 250 | |
Regioni | 273 – 314 | A2By similarityAdd BLAST | 42 |
Sequence similaritiesi
Belongs to the ribosome-inactivating protein family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR016331, Shiga-like_toxin_subunit_A |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PIRSFi | PIRSF001924, Shigella_toxin_subunit_A, 1 hit |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P09385-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKCILFKWVL CLLLGFSSVS YSREFTIDFS TQQSYVSSLN SIRTEISTPL
60 70 80 90 100
EHISQGTTSV SVINHTPPGS YFAVDIRGLD VYQARFDHLR LIIEQNNLYV
110 120 130 140 150
AGFVNTATNT FYRFSDFTHI SVPGVTTVSM TTDSSYTTLQ RVAALERSGM
160 170 180 190 200
QISRHSLVSS YLALMEFSGN TMTRDASRAV LRFVTVTAEA LRFRQIQREF
210 220 230 240 250
RQALSETAPV YTMTPGDVDL TLNWGRISNV LPEYRGEDGV RVGRISFNNI
260 270 280 290 300
SAILGTVAVI LNCHHQGARS VRAVNEESQP ECQITGDRPV IKINNTLWES
310
NTAAAFLNRK SQFLYTTGK
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 17 | S → P in strain: OX3:H21. | 1 | |
Natural varianti | 26 | T → M in strain: OX3:H21. | 1 | |
Natural varianti | 277 | E → D in strain: FLY16 and CS1718. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07865 Genomic DNA Translation: CAA30714.1 M59432 Unassigned DNA Translation: AAA19623.1 L11079 Unassigned DNA Translation: AAA16362.1 Y10775 Genomic DNA Translation: CAA71747.1 AB017524 Genomic DNA Translation: BAA33759.1 AB015057 Genomic DNA Translation: BAA34372.1 AF461167 Genomic DNA Translation: AAM70033.1 AB048239 Genomic DNA Translation: BAB83026.1 AB048240 Genomic DNA Translation: BAB83028.1 AY443052 Genomic DNA Translation: AAS07596.1 AF125520 Genomic DNA Translation: AAD25445.1 |
PIRi | I76713 S01032 |
RefSeqi | NP_049500.1, NC_000924.1 |
Genome annotation databases
GeneIDi | 1261950 |
KEGGi | vg:1261950 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07865 Genomic DNA Translation: CAA30714.1 M59432 Unassigned DNA Translation: AAA19623.1 L11079 Unassigned DNA Translation: AAA16362.1 Y10775 Genomic DNA Translation: CAA71747.1 AB017524 Genomic DNA Translation: BAA33759.1 AB015057 Genomic DNA Translation: BAA34372.1 AF461167 Genomic DNA Translation: AAM70033.1 AB048239 Genomic DNA Translation: BAB83026.1 AB048240 Genomic DNA Translation: BAB83028.1 AY443052 Genomic DNA Translation: AAS07596.1 AF125520 Genomic DNA Translation: AAD25445.1 |
PIRi | I76713 S01032 |
RefSeqi | NP_049500.1, NC_000924.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R4P | X-ray | 1.77 | A | 23-319 | [»] | |
2GA4 | X-ray | 1.80 | A | 23-319 | [»] | |
SMRi | P09385 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P09385, 1 interactor |
Genome annotation databases
GeneIDi | 1261950 |
KEGGi | vg:1261950 |
Miscellaneous databases
EvolutionaryTracei | P09385 |
Family and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR016331, Shiga-like_toxin_subunit_A |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PIRSFi | PIRSF001924, Shigella_toxin_subunit_A, 1 hit |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | STXA_BP933 | |
Accessioni | P09385Primary (citable) accession number: P09385 Secondary accession number(s): Q9R398 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | February 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families