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Entry version 183 (10 Feb 2021)
Sequence version 1 (01 Jul 1989)
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Protein

HTH-type transcriptional activator RhaS

Gene

rhaS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Activates expression of the rhaBAD and rhaT operons.UniRule annotation2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi191 – 212H-T-H motifUniRule annotationAdd BLAST22
DNA bindingi239 – 262H-T-H motifUniRule annotationAdd BLAST24

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • DNA-binding transcription factor activity Source: EcoCyc
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding
Biological processRhamnose metabolism, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:PD00221

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
HTH-type transcriptional activator RhaSUniRule annotation
Alternative name(s):
L-rhamnose operon regulatory protein RhaSUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rhaSUniRule annotation
Synonyms:rhaC2
Ordered Locus Names:b3905, JW3876
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi201L → A: 48% of wild-type activity. 1 Publication1
Mutagenesisi202R → A: 4% of wild-type activity. 1 Publication1
Mutagenesisi205H → A: 28% of wild-type activity. 1 Publication1
Mutagenesisi206R → A: 0.1% of wild-type activity. 1 Publication1
Mutagenesisi246C → A: 66% of wild-type activity. 1 Publication1
Mutagenesisi247G → A: 6.2% of wild-type activity. 1 Publication1
Mutagenesisi247G → R: 0.9% of wild-type activity. 1 Publication1
Mutagenesisi248F → A: 0.9% of wild-type activity. 1 Publication1
Mutagenesisi248F → V: 3.3% of wild-type activity. 1 Publication1
Mutagenesisi249S → G: 93% of wild-type activity. 1 Publication1
Mutagenesisi250D → A: 8.3% of wild-type activity. 1 Publication1
Mutagenesisi251S → G: 87% of wild-type activity. 1 Publication1
Mutagenesisi252N → A: 31% of wild-type activity. 1 Publication1
Mutagenesisi252N → H: 0.4% of wild-type activity. 1 Publication1
Mutagenesisi252N → I: 0.3% of wild-type activity. 1 Publication1
Mutagenesisi252N → S: 37% of wild-type activity. 1 Publication1
Mutagenesisi253H → A: 48% of wild-type activity. 1 Publication1
Mutagenesisi253H → Q: 32% of wild-type activity. 1 Publication1
Mutagenesisi254F → A: 1.2% of wild-type activity. 1 Publication1
Mutagenesisi255S → A: 80% of wild-type activity. 1 Publication1
Mutagenesisi256T → A: 61% of wild-type activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001945631 – 278HTH-type transcriptional activator RhaSAdd BLAST278

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P09377

PRoteomics IDEntifications database

More...
PRIDEi
P09377

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Induced by RhaR in response to L-rhamnose. Binding of the cAMP receptor protein (CRP) is required for full expression.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Binds DNA as a dimer.

UniRule annotationCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei241Interaction with sigma-70UniRule annotation1
Sitei250Interaction with sigma-70UniRule annotation1

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4260998, 4 interactors
852694, 5 interactors

Database of interacting proteins

More...
DIPi
DIP-10694N

Protein interaction database and analysis system

More...
IntActi
P09377, 24 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3905

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P09377

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini174 – 272HTH araC/xylS-typeUniRule annotationAdd BLAST99

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG4977, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_000445_88_5_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P09377

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P09377

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.120.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_01534, HTH_type_RhaS, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003313, AraC-bd
IPR009057, Homeobox-like_sf
IPR037923, HTH-like
IPR018060, HTH_AraC
IPR018062, HTH_AraC-typ_CS
IPR014710, RmlC-like_jellyroll
IPR020449, Tscrpt_reg_HTH_AraC-type
IPR023609, Tscrpt_reg_HTH_RhaS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02311, AraC_binding, 1 hit
PF12833, HTH_18, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00032, HTHARAC

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00342, HTH_ARAC, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF46689, SSF46689, 2 hits
SSF51215, SSF51215, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00041, HTH_ARAC_FAMILY_1, 1 hit
PS01124, HTH_ARAC_FAMILY_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P09377-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF
60 70 80 90 100
NGQPYTITGG TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ
110 120 130 140 150
LLPQELDGQY PSHWRVNHSV LQQVRQLVAQ MEQQEGENDL PSTASREILF
160 170 180 190 200
MQLLLLLRKS SLQENLENSA SRLNLLLAWL EDHFADEVNW DAVADQFSLS
210 220 230 240 250
LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT DIAYRCGFSD
260 270
SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
Length:278
Mass (Da):32,315
Last modified:July 1, 1989 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i64264514484C0D2A
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X06058 Genomic DNA Translation: CAA29452.1
L19201 Genomic DNA Translation: AAB03038.1
U00096 Genomic DNA Translation: AAC76887.1
AP009048 Genomic DNA Translation: BAE77404.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S40849

NCBI Reference Sequences

More...
RefSeqi
NP_418341.1, NC_000913.3
WP_000217137.1, NZ_SSZK01000014.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76887; AAC76887; b3905
BAE77404; BAE77404; BAE77404

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
57732253
948397

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3876
eco:b3905

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2800

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06058 Genomic DNA Translation: CAA29452.1
L19201 Genomic DNA Translation: AAB03038.1
U00096 Genomic DNA Translation: AAC76887.1
AP009048 Genomic DNA Translation: BAE77404.1
PIRiS40849
RefSeqiNP_418341.1, NC_000913.3
WP_000217137.1, NZ_SSZK01000014.1

3D structure databases

SMRiP09377
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4260998, 4 interactors
852694, 5 interactors
DIPiDIP-10694N
IntActiP09377, 24 interactors
STRINGi511145.b3905

Proteomic databases

PaxDbiP09377
PRIDEiP09377

Genome annotation databases

EnsemblBacteriaiAAC76887; AAC76887; b3905
BAE77404; BAE77404; BAE77404
GeneIDi57732253
948397
KEGGiecj:JW3876
eco:b3905
PATRICifig|1411691.4.peg.2800

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0836

Phylogenomic databases

eggNOGiCOG4977, Bacteria
HOGENOMiCLU_000445_88_5_6
InParanoidiP09377
PhylomeDBiP09377

Enzyme and pathway databases

BioCyciEcoCyc:PD00221

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P09377

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_01534, HTH_type_RhaS, 1 hit
InterProiView protein in InterPro
IPR003313, AraC-bd
IPR009057, Homeobox-like_sf
IPR037923, HTH-like
IPR018060, HTH_AraC
IPR018062, HTH_AraC-typ_CS
IPR014710, RmlC-like_jellyroll
IPR020449, Tscrpt_reg_HTH_AraC-type
IPR023609, Tscrpt_reg_HTH_RhaS
PfamiView protein in Pfam
PF02311, AraC_binding, 1 hit
PF12833, HTH_18, 1 hit
PRINTSiPR00032, HTHARAC
SMARTiView protein in SMART
SM00342, HTH_ARAC, 1 hit
SUPFAMiSSF46689, SSF46689, 2 hits
SSF51215, SSF51215, 1 hit
PROSITEiView protein in PROSITE
PS00041, HTH_ARAC_FAMILY_1, 1 hit
PS01124, HTH_ARAC_FAMILY_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRHAS_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P09377
Secondary accession number(s): Q2M8K2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 10, 2021
This is version 183 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome
UniProt is an ELIXIR core data resource
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