UniProtKB - P09377 (RHAS_ECOLI)
Protein
HTH-type transcriptional activator RhaS
Gene
rhaS
Organism
Escherichia coli (strain K12)
Status
Functioni
Activates expression of the rhaBAD and rhaT operons.UniRule annotation2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 191 – 212 | H-T-H motifUniRule annotationAdd BLAST | 22 | |
DNA bindingi | 239 – 262 | H-T-H motifUniRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: EcoCyc
- sequence-specific DNA binding Source: InterPro
GO - Biological processi
- positive regulation of transcription, DNA-templated Source: UniProtKB-UniRule
- regulation of transcription, DNA-templated Source: EcoCyc
- rhamnose metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Rhamnose metabolism, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00221 |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional activator RhaSUniRule annotationAlternative name(s): L-rhamnose operon regulatory protein RhaSUniRule annotation |
Gene namesi | Name:rhaSUniRule annotation Synonyms:rhaC2 Ordered Locus Names:b3905, JW3876 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 201 | L → A: 48% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 202 | R → A: 4% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 205 | H → A: 28% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 206 | R → A: 0.1% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 246 | C → A: 66% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 247 | G → A: 6.2% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 247 | G → R: 0.9% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 248 | F → A: 0.9% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 248 | F → V: 3.3% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 249 | S → G: 93% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 250 | D → A: 8.3% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 251 | S → G: 87% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 252 | N → A: 31% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 252 | N → H: 0.4% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 252 | N → I: 0.3% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 252 | N → S: 37% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 253 | H → A: 48% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 253 | H → Q: 32% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 254 | F → A: 1.2% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 255 | S → A: 80% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 256 | T → A: 61% of wild-type activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194563 | 1 – 278 | HTH-type transcriptional activator RhaSAdd BLAST | 278 |
Proteomic databases
PaxDbi | P09377 |
PRIDEi | P09377 |
Expressioni
Inductioni
Induced by RhaR in response to L-rhamnose. Binding of the cAMP receptor protein (CRP) is required for full expression.1 Publication
Interactioni
Subunit structurei
Binds DNA as a dimer.
UniRule annotationCuratedSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 241 | Interaction with sigma-70UniRule annotation | 1 | |
Sitei | 250 | Interaction with sigma-70UniRule annotation | 1 |
Protein-protein interaction databases
BioGRIDi | 4260998, 4 interactors 852694, 5 interactors |
DIPi | DIP-10694N |
IntActi | P09377, 24 interactors |
STRINGi | 511145.b3905 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 174 – 272 | HTH araC/xylS-typeUniRule annotationAdd BLAST | 99 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG4977, Bacteria |
HOGENOMi | CLU_000445_88_5_6 |
InParanoidi | P09377 |
PhylomeDBi | P09377 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
HAMAPi | MF_01534, HTH_type_RhaS, 1 hit |
InterProi | View protein in InterPro IPR003313, AraC-bd IPR009057, Homeobox-like_sf IPR037923, HTH-like IPR018060, HTH_AraC IPR018062, HTH_AraC-typ_CS IPR014710, RmlC-like_jellyroll IPR020449, Tscrpt_reg_HTH_AraC-type IPR023609, Tscrpt_reg_HTH_RhaS |
Pfami | View protein in Pfam PF02311, AraC_binding, 1 hit PF12833, HTH_18, 1 hit |
PRINTSi | PR00032, HTHARAC |
SMARTi | View protein in SMART SM00342, HTH_ARAC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 2 hits SSF51215, SSF51215, 1 hit |
PROSITEi | View protein in PROSITE PS00041, HTH_ARAC_FAMILY_1, 1 hit PS01124, HTH_ARAC_FAMILY_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P09377-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF
60 70 80 90 100
NGQPYTITGG TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ
110 120 130 140 150
LLPQELDGQY PSHWRVNHSV LQQVRQLVAQ MEQQEGENDL PSTASREILF
160 170 180 190 200
MQLLLLLRKS SLQENLENSA SRLNLLLAWL EDHFADEVNW DAVADQFSLS
210 220 230 240 250
LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT DIAYRCGFSD
260 270
SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06058 Genomic DNA Translation: CAA29452.1 L19201 Genomic DNA Translation: AAB03038.1 U00096 Genomic DNA Translation: AAC76887.1 AP009048 Genomic DNA Translation: BAE77404.1 |
PIRi | S40849 |
RefSeqi | NP_418341.1, NC_000913.3 WP_000217137.1, NZ_SSZK01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC76887; AAC76887; b3905 BAE77404; BAE77404; BAE77404 |
GeneIDi | 48133262 948397 |
KEGGi | ecj:JW3876 eco:b3905 |
PATRICi | fig|1411691.4.peg.2800 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06058 Genomic DNA Translation: CAA29452.1 L19201 Genomic DNA Translation: AAB03038.1 U00096 Genomic DNA Translation: AAC76887.1 AP009048 Genomic DNA Translation: BAE77404.1 |
PIRi | S40849 |
RefSeqi | NP_418341.1, NC_000913.3 WP_000217137.1, NZ_SSZK01000014.1 |
3D structure databases
SMRi | P09377 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260998, 4 interactors 852694, 5 interactors |
DIPi | DIP-10694N |
IntActi | P09377, 24 interactors |
STRINGi | 511145.b3905 |
Proteomic databases
PaxDbi | P09377 |
PRIDEi | P09377 |
Genome annotation databases
EnsemblBacteriai | AAC76887; AAC76887; b3905 BAE77404; BAE77404; BAE77404 |
GeneIDi | 48133262 948397 |
KEGGi | ecj:JW3876 eco:b3905 |
PATRICi | fig|1411691.4.peg.2800 |
Organism-specific databases
EchoBASEi | EB0836 |
Phylogenomic databases
eggNOGi | COG4977, Bacteria |
HOGENOMi | CLU_000445_88_5_6 |
InParanoidi | P09377 |
PhylomeDBi | P09377 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00221 |
Miscellaneous databases
PROi | PR:P09377 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
HAMAPi | MF_01534, HTH_type_RhaS, 1 hit |
InterProi | View protein in InterPro IPR003313, AraC-bd IPR009057, Homeobox-like_sf IPR037923, HTH-like IPR018060, HTH_AraC IPR018062, HTH_AraC-typ_CS IPR014710, RmlC-like_jellyroll IPR020449, Tscrpt_reg_HTH_AraC-type IPR023609, Tscrpt_reg_HTH_RhaS |
Pfami | View protein in Pfam PF02311, AraC_binding, 1 hit PF12833, HTH_18, 1 hit |
PRINTSi | PR00032, HTHARAC |
SMARTi | View protein in SMART SM00342, HTH_ARAC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 2 hits SSF51215, SSF51215, 1 hit |
PROSITEi | View protein in PROSITE PS00041, HTH_ARAC_FAMILY_1, 1 hit PS01124, HTH_ARAC_FAMILY_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHAS_ECOLI | |
Accessioni | P09377Primary (citable) accession number: P09377 Secondary accession number(s): Q2M8K2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |