grpE

Functionⁱ

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.2 Publications

GO - Molecular functionⁱ

adenyl-nucleotide exchange factor activity
Source: EcoCyc
Inferred from direct assayⁱ
- 1826368
- 8599944
chaperone binding Source: InterPro
protein domain specific binding
Source: CAFA
Inferred from physical interactionⁱ
- 9103205
protein homodimerization activity
Source: CAFA
Inferred from direct assayⁱ
- 9103205
unfolded protein binding Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protein-containing complex assembly
Source: CAFA
Inferred from mutant phenotypeⁱ
- 9103205
protein folding Source: InterPro
response to heat
Source: EcoCyc
Inferred from direct assayⁱ
- 11084044

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone
Biological process	Stress response

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG10416-MONOMER MetaCyc:EG10416-MONOMER

BioCycⁱ

EcoCyc:EG10416-MONOMER
MetaCyc:EG10416-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein GrpE Alternative name(s): HSP-70 cofactor HSP24 Heat shock protein B25.3
Gene namesⁱ	Name:grpE Ordered Locus Names:b2614, JW2594
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10416 grpE

EcoGeneⁱ

EG10416 grpE

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytoplasm
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- 16858726
cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323
protein-containing complex
Source: CAFA
Inferred from direct assayⁱ
- 9103205

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	73	R → A: Great decrease in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	74	R → A: Great decrease in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	82	K → A: Great decrease in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	86	F → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	104	R → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	107	E → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	108	V → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	122	G → D: Temperature-sensitive phenotype. 1 Publication	1
Mutagenesisⁱ	149	L → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	151	P → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	155	Q → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	157	I → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	159	M → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	174	M → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	183	R → A: Loss of ability to interact with DnaK. 2 Publications	1
Mutagenesisⁱ	186	R → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	189	M → A: No effect in ability to interact with DnaK. 1 Publication	1
Mutagenesisⁱ	192	V → A: Loss of ability to interact with DnaK. 2 Publications	1

Chemistry databases

ChEMBLⁱ	CHEMBL1293284

ChEMBLⁱ

CHEMBL1293284

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000113782	1 – 197	Protein GrpEAdd BLAST		197

Proteomic databases

EPDⁱ	P09372
PaxDbⁱ	P09372
PRIDEⁱ	P09372

2D gel databases

SWISS-2DPAGEⁱ	P09372

SWISS-2DPAGEⁱ

P09372

Expressionⁱ

Inductionⁱ

By the sigma(32) subunit of RNA polymerase.

Interactionⁱ

Subunit structureⁱ

Homodimer.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	183	Interaction with DnaK		1

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
dnaK	P0A6Y8	10	EBI-547441,EBI-542092
groL	P0A6F5	3	EBI-547441,EBI-543750
mntH	P0A769	3	EBI-547441,EBI-551337

GO - Molecular functionⁱ

chaperone binding Source: InterPro
protein domain specific binding
Source: CAFA
Inferred from physical interactionⁱ
- 9103205
protein homodimerization activity
Source: CAFA
Inferred from direct assayⁱ
- 9103205
unfolded protein binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4260616, 160 interactors 851433, 1 interactor
Database of interacting proteins More...DIPⁱ	DIP-6141N
IntActⁱ	P09372, 50 interactors
STRINGⁱ	316385.ECDH10B_2780

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	39 – 85	Combined sources	47
Helixⁱ	88 – 90	Combined sources	3
Helixⁱ	91 – 94	Combined sources	4
Helixⁱ	96 – 107	Combined sources	12
Helixⁱ	117 – 134	Combined sources	18
Turnⁱ	135 – 138	Combined sources	4
Beta strandⁱ	139 – 142	Combined sources	4
Beta strandⁱ	146 – 148	Combined sources	3
Beta strandⁱ	153 – 161	Combined sources	9
Beta strandⁱ	163 – 165	Combined sources	3
Beta strandⁱ	169 – 175	Combined sources	7
Beta strandⁱ	177 – 180	Combined sources	4
Beta strandⁱ	183 – 186	Combined sources	4
Beta strandⁱ	188 – 194	Combined sources	7

Show more details

3D structure databases

Database of protein disorder More...DisProtⁱ	DP00103
ProteinModelPortalⁱ	P09372
SMRⁱ	P09372
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P09372

EvolutionaryTraceⁱ

P09372

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the GrpE family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105K90 Bacteria COG0576 LUCA
HOGENOMⁱ	HOG000252084
InParanoidⁱ	P09372
KEGG Orthology (KO) More...KOⁱ	K03687
OMAⁱ	YAYEKIA
PhylomeDBⁱ	P09372

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00446 GrpE, 1 hit
Gene3Dⁱ	2.30.22.10, 1 hit 3.90.20.20, 1 hit
HAMAPⁱ	MF_01151 GrpE, 1 hit
InterProⁱ	View protein in InterPro IPR000740 GrpE IPR013805 GrpE_coiled_coil IPR009012 GrpE_head
PANTHERⁱ	PTHR21237 PTHR21237, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01025 GrpE, 1 hit
PRINTSⁱ	PR00773 GRPEPROTEIN
SUPFAMⁱ	SSF51064 SSF51064, 1 hit SSF58014 SSF58014, 1 hit
PROSITEⁱ	View protein in PROSITE PS01071 GRPE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P09372-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKVANLEAQ 
        60         70         80         90        100
LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID 
       110        120        130        140        150
SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD 
       160        170        180        190 
PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA

Length:197

Mass (Da):21,798

Last modified:July 1, 1989 - v1

Checksum:ⁱCDC4CD9D08AD4BEF

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X07863 Genomic DNA Translation: CAA30711.1 U00096 Genomic DNA Translation: AAC75663.1 AP009048 Genomic DNA Translation: BAA16498.1
PIRⁱ	S01240
NCBI Reference Sequences More...RefSeqⁱ	NP_417104.1, NC_000913.3 WP_001393454.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75663; AAC75663; b2614 BAA16498; BAA16498; BAA16498
GeneIDⁱ	947097
KEGGⁱ	ecj:JW2594 eco:b2614
PATRICⁱ	fig\|1411691.4.peg.4126

Protein	Similar proteins		Species	Score	Length	Source
P09372	Protein GrpE		ECOBW		197	UniRef100_C4ZYN1
Protein GrpE		ECODH		197
Heat shock protein		ECOLX		197
Protein GrpE		Escherichia coli 3-373-03_S4_C2		197
UPI00064C79F5		NEIGO		108

Protein	Similar proteins		Species	Score	Length	Source
P09372	Protein GrpE		ECOBW		197	UniRef90_C4ZYN1
Protein GrpE		ECODH		197
Protein GrpE		ECO45		197
Protein GrpE		ECO81		197
Protein GrpE		ECOUT		197
+275

Protein	Similar proteins		Species	Score	Length	Source
P09372	Protein GrpE		ECOBW		197	UniRef50_C4ZYN1
Protein GrpE		ECODH		197
Protein GrpE		SHIF8		197
Protein GrpE		ECO5E		197
Protein GrpE		ECO45		197
+354

Entry informationⁱ

Entry nameⁱ	GRPE_ECOLI
Accessionⁱ	P09372Primary (citable) accession number: P09372
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 1, 1989
	Last modified:	April 25, 2018
	This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P09372 (GRPE_ECOLI)

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Protein GrpE

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Sites

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ