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Protein

Protein GrpE

Gene

grpE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.2 Publications

GO - Molecular functioni

  • adenyl-nucleotide exchange factor activity Source: EcoCyc
  • chaperone binding Source: InterPro
  • protein domain specific binding Source: CAFA
  • protein homodimerization activity Source: CAFA
  • unfolded protein binding Source: GO_Central

GO - Biological processi

  • protein-containing complex assembly Source: CAFA
  • protein folding Source: InterPro
  • response to heat Source: EcoCyc

Keywordsi

Molecular functionChaperone
Biological processStress response

Enzyme and pathway databases

BioCyciEcoCyc:EG10416-MONOMER
MetaCyc:EG10416-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GrpE
Alternative name(s):
HSP-70 cofactor
HSP24
Heat shock protein B25.3
Gene namesi
Name:grpE
Ordered Locus Names:b2614, JW2594
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10416 grpE

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73R → A: Great decrease in ability to interact with DnaK. 1 Publication1
Mutagenesisi74R → A: Great decrease in ability to interact with DnaK. 1 Publication1
Mutagenesisi82K → A: Great decrease in ability to interact with DnaK. 1 Publication1
Mutagenesisi86F → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi104R → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi107E → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi108V → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi122G → D: Temperature-sensitive phenotype. 1 Publication1
Mutagenesisi149L → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi151P → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi155Q → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi157I → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi159M → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi174M → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi183R → A: Loss of ability to interact with DnaK. 2 Publications1
Mutagenesisi186R → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi189M → A: No effect in ability to interact with DnaK. 1 Publication1
Mutagenesisi192V → A: Loss of ability to interact with DnaK. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL1293284

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001137821 – 197Protein GrpEAdd BLAST197

Proteomic databases

EPDiP09372
PaxDbiP09372
PRIDEiP09372

2D gel databases

SWISS-2DPAGEiP09372

Expressioni

Inductioni

By the sigma(32) subunit of RNA polymerase.

Interactioni

Subunit structurei

Homodimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei183Interaction with DnaK1

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4260616, 160 interactors
851433, 1 interactor
DIPiDIP-6141N
IntActiP09372, 50 interactors
STRINGi316385.ECDH10B_2780

Structurei

Secondary structure

1197
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00103
ProteinModelPortaliP09372
SMRiP09372
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09372

Family & Domainsi

Sequence similaritiesi

Belongs to the GrpE family.Curated

Phylogenomic databases

eggNOGiENOG4105K90 Bacteria
COG0576 LUCA
HOGENOMiHOG000252084
InParanoidiP09372
KOiK03687
OMAiYAYEKIA
PhylomeDBiP09372

Family and domain databases

CDDicd00446 GrpE, 1 hit
Gene3Di2.30.22.10, 1 hit
3.90.20.20, 1 hit
HAMAPiMF_01151 GrpE, 1 hit
InterProiView protein in InterPro
IPR000740 GrpE
IPR013805 GrpE_coiled_coil
IPR009012 GrpE_head
PANTHERiPTHR21237 PTHR21237, 1 hit
PfamiView protein in Pfam
PF01025 GrpE, 1 hit
PRINTSiPR00773 GRPEPROTEIN
SUPFAMiSSF51064 SSF51064, 1 hit
SSF58014 SSF58014, 1 hit
PROSITEiView protein in PROSITE
PS01071 GRPE, 1 hit

Sequencei

Sequence statusi: Complete.

P09372-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKVANLEAQ
60 70 80 90 100
LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID
110 120 130 140 150
SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD
160 170 180 190
PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Length:197
Mass (Da):21,798
Last modified:July 1, 1989 - v1
Checksum:iCDC4CD9D08AD4BEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07863 Genomic DNA Translation: CAA30711.1
U00096 Genomic DNA Translation: AAC75663.1
AP009048 Genomic DNA Translation: BAA16498.1
PIRiS01240
RefSeqiNP_417104.1, NC_000913.3
WP_001393454.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75663; AAC75663; b2614
BAA16498; BAA16498; BAA16498
GeneIDi947097
KEGGiecj:JW2594
eco:b2614
PATRICifig|1411691.4.peg.4126

Similar proteinsi

Entry informationi

Entry nameiGRPE_ECOLI
AccessioniPrimary (citable) accession number: P09372
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 25, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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