UniProtKB - P09372 (GRPE_ECOLI)
Protein GrpE
grpE
Functioni
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
2 PublicationsGO - Molecular functioni
- adenyl-nucleotide exchange factor activity Source: EcoCyc
- chaperone binding Source: InterPro
- protein domain specific binding Source: CAFA
- protein homodimerization activity Source: CAFA
- unfolded protein binding Source: GO_Central
GO - Biological processi
- protein-containing complex assembly Source: CAFA
- protein folding Source: InterPro
- response to heat Source: EcoCyc
Keywordsi
Molecular function | Chaperone |
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10416-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Protein GrpEAlternative name(s): HSP-70 cofactor HSP24 Heat shock protein B25.3 |
Gene namesi | Name:grpE Ordered Locus Names:b2614, JW2594 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: EcoCyc
Other locations
- cytoplasm Source: UniProtKB
- protein-containing complex Source: CAFA
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | R → A: Great decrease in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 74 | R → A: Great decrease in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 82 | K → A: Great decrease in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 86 | F → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 104 | R → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 107 | E → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 108 | V → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 122 | G → D: Temperature-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 149 | L → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 151 | P → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 155 | Q → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 157 | I → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 159 | M → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 174 | M → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 183 | R → A: Loss of ability to interact with DnaK. 2 Publications | 1 | |
Mutagenesisi | 186 | R → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 189 | M → A: No effect in ability to interact with DnaK. 1 Publication | 1 | |
Mutagenesisi | 192 | V → A: Loss of ability to interact with DnaK. 2 Publications | 1 |
Chemistry databases
ChEMBLi | CHEMBL1293284 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000113782 | 1 – 197 | Protein GrpEAdd BLAST | 197 |
Proteomic databases
jPOSTi | P09372 |
PaxDbi | P09372 |
PRIDEi | P09372 |
2D gel databases
SWISS-2DPAGEi | P09372 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 183 | Interaction with DnaK | 1 |
Binary interactionsi
P09372
With | #Exp. | IntAct |
---|---|---|
dnaK [P0A6Y8] | 10 | EBI-547441,EBI-542092 |
groEL [P0A6F5] | 3 | EBI-547441,EBI-543750 |
mntH [P0A769] | 3 | EBI-547441,EBI-551337 |
GO - Molecular functioni
- chaperone binding Source: InterPro
- protein domain specific binding Source: CAFA
- protein homodimerization activity Source: CAFA
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 4260616, 160 interactors 851433, 2 interactors |
DIPi | DIP-6141N |
IntActi | P09372, 50 interactors |
STRINGi | 511145.b2614 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P09372 |
SMRi | P09372 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09372 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | DisorderedSequence analysisAdd BLAST | 40 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 16 – 40 | Basic and acidic residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0576, Bacteria |
HOGENOMi | CLU_057217_6_0_6 |
InParanoidi | P09372 |
OMAi | YAYEKIA |
PhylomeDBi | P09372 |
Family and domain databases
CDDi | cd00446, GrpE, 1 hit |
DisProti | DP00103 |
Gene3Di | 2.30.22.10, 1 hit 3.90.20.20, 1 hit |
HAMAPi | MF_01151, GrpE, 1 hit |
InterProi | View protein in InterPro IPR000740, GrpE IPR013805, GrpE_coiled_coil IPR009012, GrpE_head |
PANTHERi | PTHR21237, PTHR21237, 1 hit |
Pfami | View protein in Pfam PF01025, GrpE, 1 hit |
PRINTSi | PR00773, GRPEPROTEIN |
SUPFAMi | SSF51064, SSF51064, 1 hit SSF58014, SSF58014, 1 hit |
PROSITEi | View protein in PROSITE PS01071, GRPE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKVANLEAQ
60 70 80 90 100
LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID
110 120 130 140 150
SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD
160 170 180 190
PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07863 Genomic DNA Translation: CAA30711.1 U00096 Genomic DNA Translation: AAC75663.1 AP009048 Genomic DNA Translation: BAA16498.1 |
PIRi | S01240 |
RefSeqi | NP_417104.1, NC_000913.3 WP_001393454.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75663; AAC75663; b2614 BAA16498; BAA16498; BAA16498 |
GeneIDi | 947097 |
KEGGi | ecj:JW2594 eco:b2614 |
PATRICi | fig|1411691.4.peg.4126 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07863 Genomic DNA Translation: CAA30711.1 U00096 Genomic DNA Translation: AAC75663.1 AP009048 Genomic DNA Translation: BAA16498.1 |
PIRi | S01240 |
RefSeqi | NP_417104.1, NC_000913.3 WP_001393454.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DKG | X-ray | 2.80 | A/B | 1-197 | [»] | |
AlphaFoldDBi | P09372 | |||||
SMRi | P09372 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260616, 160 interactors 851433, 2 interactors |
DIPi | DIP-6141N |
IntActi | P09372, 50 interactors |
STRINGi | 511145.b2614 |
Chemistry databases
ChEMBLi | CHEMBL1293284 |
2D gel databases
SWISS-2DPAGEi | P09372 |
Proteomic databases
jPOSTi | P09372 |
PaxDbi | P09372 |
PRIDEi | P09372 |
Genome annotation databases
EnsemblBacteriai | AAC75663; AAC75663; b2614 BAA16498; BAA16498; BAA16498 |
GeneIDi | 947097 |
KEGGi | ecj:JW2594 eco:b2614 |
PATRICi | fig|1411691.4.peg.4126 |
Organism-specific databases
EchoBASEi | EB0411 |
Phylogenomic databases
eggNOGi | COG0576, Bacteria |
HOGENOMi | CLU_057217_6_0_6 |
InParanoidi | P09372 |
OMAi | YAYEKIA |
PhylomeDBi | P09372 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10416-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P09372 |
PROi | PR:P09372 |
Family and domain databases
CDDi | cd00446, GrpE, 1 hit |
DisProti | DP00103 |
Gene3Di | 2.30.22.10, 1 hit 3.90.20.20, 1 hit |
HAMAPi | MF_01151, GrpE, 1 hit |
InterProi | View protein in InterPro IPR000740, GrpE IPR013805, GrpE_coiled_coil IPR009012, GrpE_head |
PANTHERi | PTHR21237, PTHR21237, 1 hit |
Pfami | View protein in Pfam PF01025, GrpE, 1 hit |
PRINTSi | PR00773, GRPEPROTEIN |
SUPFAMi | SSF51064, SSF51064, 1 hit SSF58014, SSF58014, 1 hit |
PROSITEi | View protein in PROSITE PS01071, GRPE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRPE_ECOLI | |
Accessioni | P09372Primary (citable) accession number: P09372 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | May 25, 2022 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families