UniProtKB - P09348 (MOTA_ECOLI)
Motility protein A
motA
Functioni
MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel. Overexpression of MotA, with or without MotB, restores motility in a pdeH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.
1 PublicationGO - Molecular functioni
- proton channel activity Source: EcoCyc
GO - Biological processi
- bacterial-type flagellum-dependent cell motility Source: EcoliWiki
- bacterial-type flagellum-dependent swarming motility Source: GO_Central
- chemotaxis Source: ComplexPortal
- proton transmembrane transport Source: EcoCyc
Keywordsi
Biological process | Chemotaxis, Flagellar rotation, Hydrogen ion transport, Ion transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN |
Protein family/group databases
TCDBi | 1.A.30.1.1, the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Motility protein AAlternative name(s): Chemotaxis protein MotA |
Gene namesi | Name:motA Synonyms:flaJ Ordered Locus Names:b1890, JW1879 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- bacterial-type flagellum stator complex Source: ComplexPortal
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- bacterial-type flagellum Source: ComplexPortal
- integral component of membrane Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 2 – 21 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 22 – 33 | PeriplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 34 – 51 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 52 – 170 | CytoplasmicSequence analysisAdd BLAST | 119 | |
Transmembranei | 171 – 191 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 192 – 200 | PeriplasmicSequence analysis | 9 | |
Transmembranei | 201 – 222 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 223 – 295 | CytoplasmicSequence analysisAdd BLAST | 73 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 93 | G → E: Suppresses pdeH disruption motility mutant, swimming velocity is wild-type. 1 Publication | 1 | |
Mutagenesisi | 93 | G → R: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. 1 Publication | 1 | |
Mutagenesisi | 93 | G → V: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication | 1 | |
Mutagenesisi | 96 | S → L: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:6533 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189572 | 1 – 295 | Motility protein AAdd BLAST | 295 |
Proteomic databases
PaxDbi | P09348 |
PRIDEi | P09348 |
Interactioni
Subunit structurei
Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels.
Interacts with FliG.
Interacts with flagellar brake protein YcgR in the flagellar basal bodies (PubMed:20303158). In another study (PubMed:20346719) YcgR was not seen to interact with MotA, but instead with FliM and FliG, also in the flagellar basal body.
2 PublicationsBinary interactionsi
P09348
With | #Exp. | IntAct |
---|---|---|
motB [P0AF06] | 2 | EBI-557926,EBI-1117399 |
Protein-protein interaction databases
BioGRIDi | 4262245, 486 interactors |
ComplexPortali | CPX-5884, Flagellar motor stator complex |
DIPi | DIP-10244N |
IntActi | P09348, 3 interactors |
STRINGi | 511145.b1890 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1291, Bacteria |
HOGENOMi | CLU_068213_0_0_6 |
InParanoidi | P09348 |
OMAi | EIETHHQ |
PhylomeDBi | P09348 |
Family and domain databases
InterProi | View protein in InterPro IPR000540, Flag_MotA_CS IPR022522, Flagellar_motor_stator_MotA IPR002898, MotA_ExbB_proton_chnl |
PANTHERi | PTHR30433:SF4, PTHR30433:SF4, 1 hit |
Pfami | View protein in Pfam PF01618, MotA_ExbB, 1 hit |
TIGRFAMsi | TIGR03818, MotA1, 1 hit |
PROSITEi | View protein in PROSITE PS01307, MOTA, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLILLGYLVV LGTVFGGYLM TGGSLGALYQ PAELVIIAGA GIGSFIVGNN
60 70 80 90 100
GKAIKGTLKA LPLLFRRSKY TKAMYMDLLA LLYRLMAKSR QMGMFSLERD
110 120 130 140 150
IENPRESEIF ASYPRILADS VMLDFIVDYL RLIISGHMNT FEIEALMDEE
160 170 180 190 200
IETHESEAEV PANSLALVGD SLPAFGIVAA VMGVVHALGS ADRPAAELGA
210 220 230 240 250
LIAHAMVGTF LGILLAYGFI SPLATVLRQK SAETSKMMQC VKVTLLSNLN
260 270 280 290
GYAPPIAVEF GRKTLYSSER PSFIELEEHV RAVKNPQQQT TTEEA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01652 Genomic DNA Translation: AAA24177.1 U00096 Genomic DNA Translation: AAC74960.1 AP009048 Genomic DNA Translation: BAA15711.1 |
PIRi | A30279, QRECMA |
RefSeqi | NP_416404.1, NC_000913.3 WP_000906340.1, NZ_STEB01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC74960; AAC74960; b1890 BAA15711; BAA15711; BAA15711 |
GeneIDi | 947564 |
KEGGi | ecj:JW1879 eco:b1890 |
PATRICi | fig|1411691.4.peg.357 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01652 Genomic DNA Translation: AAA24177.1 U00096 Genomic DNA Translation: AAC74960.1 AP009048 Genomic DNA Translation: BAA15711.1 |
PIRi | A30279, QRECMA |
RefSeqi | NP_416404.1, NC_000913.3 WP_000906340.1, NZ_STEB01000026.1 |
3D structure databases
AlphaFoldDBi | P09348 |
SMRi | P09348 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262245, 486 interactors |
ComplexPortali | CPX-5884, Flagellar motor stator complex |
DIPi | DIP-10244N |
IntActi | P09348, 3 interactors |
STRINGi | 511145.b1890 |
Protein family/group databases
TCDBi | 1.A.30.1.1, the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily |
Proteomic databases
PaxDbi | P09348 |
PRIDEi | P09348 |
Genome annotation databases
EnsemblBacteriai | AAC74960; AAC74960; b1890 BAA15711; BAA15711; BAA15711 |
GeneIDi | 947564 |
KEGGi | ecj:JW1879 eco:b1890 |
PATRICi | fig|1411691.4.peg.357 |
Organism-specific databases
EchoBASEi | EB0596 |
Phylogenomic databases
eggNOGi | COG1291, Bacteria |
HOGENOMi | CLU_068213_0_0_6 |
InParanoidi | P09348 |
OMAi | EIETHHQ |
PhylomeDBi | P09348 |
Enzyme and pathway databases
BioCyci | EcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN |
Miscellaneous databases
PHI-basei | PHI:6533 |
PROi | PR:P09348 |
Family and domain databases
InterProi | View protein in InterPro IPR000540, Flag_MotA_CS IPR022522, Flagellar_motor_stator_MotA IPR002898, MotA_ExbB_proton_chnl |
PANTHERi | PTHR30433:SF4, PTHR30433:SF4, 1 hit |
Pfami | View protein in Pfam PF01618, MotA_ExbB, 1 hit |
TIGRFAMsi | TIGR03818, MotA1, 1 hit |
PROSITEi | View protein in PROSITE PS01307, MOTA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MOTA_ECOLI | |
Accessioni | P09348Primary (citable) accession number: P09348 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families