motA

Functionⁱ

MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel. Overexpression of MotA, with or without MotB, restores motility in a pdeH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

GO - Biological processⁱ

bacterial-type flagellum-dependent cell motility
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 2828314
bacterial-type flagellum-dependent swarming motility
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
chemotaxis Source: UniProtKB-KW
ion transport Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Chemotaxis, Flagellar rotation, Hydrogen ion transport, Ion transport, Transport

Biological process

Chemotaxis, Flagellar rotation, Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCycⁱ	EcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN

BioCycⁱ

EcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN

Protein family/group databases

TCDBⁱ	1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

TCDBⁱ

1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Motility protein A Alternative name(s): Chemotaxis protein MotA
Gene namesⁱ	Name:motA Synonyms:flaJ Ordered Locus Names:b1890, JW1879
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10601 motA

EcoGeneⁱ

EG10601 motA

Subcellular locationⁱ

Cell inner membrane ; Multi-pass membrane protein

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	2 – 21	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	22 – 33	PeriplasmicSequence analysisAdd BLAST	12
Transmembraneⁱ	34 – 51	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	52 – 170	CytoplasmicSequence analysisAdd BLAST	119
Transmembraneⁱ	171 – 191	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	192 – 200	PeriplasmicSequence analysis	9
Transmembraneⁱ	201 – 222	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	223 – 295	CytoplasmicSequence analysisAdd BLAST	73

GO - Cellular componentⁱ

bacterial-type flagellum stator complex
Source: CACAO
Inferred from direct assayⁱ
- 14705928
integral component of membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 2828314
plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 15919996
- 17309111

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	93	G → E: Suppresses pdeH disruption motility mutant, swimming velocity is wild-type. 1 Publication	1
Mutagenesisⁱ	93	G → R: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. 1 Publication	1
Mutagenesisⁱ	93	G → V: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication	1
Mutagenesisⁱ	96	S → L: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000189572	1 – 295	Motility protein AAdd BLAST		295

Proteomic databases

PaxDbⁱ	P09348
PRIDEⁱ	P09348

Interactionⁱ

Subunit structureⁱ

Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels. Interacts with FliG. Interacts with flagellar brake protein YcgR in the flagellar basal bodies (PubMed:20303158). In another study (PubMed:20346719) YcgR was not seen to interact with MotA, but instead with FliM and FliG, also in the flagellar basal body.2 Publications

Protein-protein interaction databases

BioGridⁱ	4262245, 486 interactors
Database of interacting proteins More...DIPⁱ	DIP-10244N
IntActⁱ	P09348, 2 interactors
STRINGⁱ	316385.ECDH10B_2031

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P09348
SMRⁱ	P09348
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the MotA family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4107RD7 Bacteria COG1291 LUCA
HOGENOMⁱ	HOG000254701
InParanoidⁱ	P09348
KEGG Orthology (KO) More...KOⁱ	K02556
PhylomeDBⁱ	P09348

Family and domain databases

InterProⁱ	View protein in InterPro IPR000540 Flag_MotA_CS IPR022522 Flagellar_motor_stator_MotA IPR002898 MotA_ExbB_proton_chnl
PANTHERⁱ	PTHR30433:SF4 PTHR30433:SF4, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01618 MotA_ExbB, 1 hit
TIGRFAMsⁱ	TIGR03818 MotA1, 1 hit
PROSITEⁱ	View protein in PROSITE PS01307 MOTA, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P09348-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLILLGYLVV LGTVFGGYLM TGGSLGALYQ PAELVIIAGA GIGSFIVGNN 
        60         70         80         90        100
GKAIKGTLKA LPLLFRRSKY TKAMYMDLLA LLYRLMAKSR QMGMFSLERD 
       110        120        130        140        150
IENPRESEIF ASYPRILADS VMLDFIVDYL RLIISGHMNT FEIEALMDEE 
       160        170        180        190        200
IETHESEAEV PANSLALVGD SLPAFGIVAA VMGVVHALGS ADRPAAELGA 
       210        220        230        240        250
LIAHAMVGTF LGILLAYGFI SPLATVLRQK SAETSKMMQC VKVTLLSNLN 
       260        270        280        290 
GYAPPIAVEF GRKTLYSSER PSFIELEEHV RAVKNPQQQT TTEEA

Length:295

Mass (Da):32,011

Last modified:July 1, 1989 - v1

Checksum:ⁱ355DC13EF63DD7D8

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J01652 Genomic DNA Translation: AAA24177.1 U00096 Genomic DNA Translation: AAC74960.1 AP009048 Genomic DNA Translation: BAA15711.1
PIRⁱ	A30279 QRECMA
NCBI Reference Sequences More...RefSeqⁱ	NP_416404.1, NC_000913.3 WP_000906340.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74960; AAC74960; b1890 BAA15711; BAA15711; BAA15711
GeneIDⁱ	947564
KEGGⁱ	ecj:JW1879 eco:b1890
PATRICⁱ	fig\|1411691.4.peg.357

Protein	Similar proteins		Species	Score	Length	Source
P09348	Chemotaxis protein MotA (Motility protein A)		ECOLX		328	UniRef100_P09348
Flagellar motor stator protein MotA (Fragment)		Staphylococcus succinus		128
Motility A domain protein (Fragment)		Shigella boydii 4444-74		141
Flagellar motor stator protein MotA		Shigella sp.		295
Flagellar motor rotation protein MotA		Klebsiella pneumoniae IS22		295
+43

Protein	Similar proteins		Species	Score	Length	Source
P09348	Chemotaxis protein MotA (Motility protein A)		ECOLX		328	UniRef90_P09348
Flagellar motor stator protein MotA (Fragment)		Staphylococcus succinus		128
Motility A domain protein (Fragment)		Shigella boydii 4444-74		141
Flagellar motor stator protein MotA		Shigella sp.		295
Flagellar motor rotation protein MotA		Klebsiella pneumoniae IS22		295
+557

Protein	Similar proteins		Species	Score	Length	Source
P09348	Motility protein A		SALTY		295	UniRef50_P09348
Chemotaxis protein MotA (Motility protein A)		ECOLX		328
Flagellar motor stator protein MotA (Fragment)		Staphylococcus succinus		128
Motility A domain protein (Fragment)		Shigella boydii 4444-74		141
Flagellar motor stator protein MotA		Shigella sp.		295
+2860

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J01652 Genomic DNA Translation: AAA24177.1 U00096 Genomic DNA Translation: AAC74960.1 AP009048 Genomic DNA Translation: BAA15711.1
PIRⁱ	A30279 QRECMA
RefSeqⁱ	NP_416404.1, NC_000913.3 WP_000906340.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P09348
SMRⁱ	P09348
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262245, 486 interactors
DIPⁱ	DIP-10244N
IntActⁱ	P09348, 2 interactors
STRINGⁱ	316385.ECDH10B_2031

Protein family/group databases

TCDBⁱ	1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

TCDBⁱ

1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

Proteomic databases

PaxDbⁱ	P09348
PRIDEⁱ	P09348

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74960; AAC74960; b1890 BAA15711; BAA15711; BAA15711
GeneIDⁱ	947564
KEGGⁱ	ecj:JW1879 eco:b1890
PATRICⁱ	fig\|1411691.4.peg.357

Organism-specific databases

EchoBASEⁱ	EB0596
EcoGeneⁱ	EG10601 motA

Phylogenomic databases

eggNOGⁱ	ENOG4107RD7 Bacteria COG1291 LUCA
HOGENOMⁱ	HOG000254701
InParanoidⁱ	P09348
KOⁱ	K02556
PhylomeDBⁱ	P09348

Enzyme and pathway databases

BioCycⁱ	EcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN

BioCycⁱ

EcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P09348

PROⁱ

PR:P09348

Family and domain databases

InterProⁱ	View protein in InterPro IPR000540 Flag_MotA_CS IPR022522 Flagellar_motor_stator_MotA IPR002898 MotA_ExbB_proton_chnl
PANTHERⁱ	PTHR30433:SF4 PTHR30433:SF4, 1 hit
Pfamⁱ	View protein in Pfam PF01618 MotA_ExbB, 1 hit
TIGRFAMsⁱ	TIGR03818 MotA1, 1 hit
PROSITEⁱ	View protein in PROSITE PS01307 MOTA, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MOTA_ECOLI
Accessionⁱ	P09348Primary (citable) accession number: P09348
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 1, 1989
	Last modified:	November 7, 2018
	This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P09348 (MOTA_ECOLI)

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Motility protein A

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ