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Protein

Motility protein A

Gene

motA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel. Overexpression of MotA, with or without MotB, restores motility in a pdeH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: EcoliWiki
  • bacterial-type flagellum-dependent swarming motility Source: GO_Central
  • chemotaxis Source: UniProtKB-KW
  • ion transport Source: UniProtKB-KW

Keywordsi

Biological processChemotaxis, Flagellar rotation, Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN

Protein family/group databases

TCDBi1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Motility protein A
Alternative name(s):
Chemotaxis protein MotA
Gene namesi
Name:motA
Synonyms:flaJ
Ordered Locus Names:b1890, JW1879
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10601 motA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2 – 21HelicalSequence analysisAdd BLAST20
Topological domaini22 – 33PeriplasmicSequence analysisAdd BLAST12
Transmembranei34 – 51HelicalSequence analysisAdd BLAST18
Topological domaini52 – 170CytoplasmicSequence analysisAdd BLAST119
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21
Topological domaini192 – 200PeriplasmicSequence analysis9
Transmembranei201 – 222HelicalSequence analysisAdd BLAST22
Topological domaini223 – 295CytoplasmicSequence analysisAdd BLAST73

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi93G → E: Suppresses pdeH disruption motility mutant, swimming velocity is wild-type. 1 Publication1
Mutagenesisi93G → R: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. 1 Publication1
Mutagenesisi93G → V: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication1
Mutagenesisi96S → L: Suppresses pdeH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001895721 – 295Motility protein AAdd BLAST295

Proteomic databases

PaxDbiP09348
PRIDEiP09348

Interactioni

Subunit structurei

Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels. Interacts with FliG. Interacts with flagellar brake protein YcgR in the flagellar basal bodies (PubMed:20303158). In another study (PubMed:20346719) YcgR was not seen to interact with MotA, but instead with FliM and FliG, also in the flagellar basal body.2 Publications

Protein-protein interaction databases

BioGridi4262245, 486 interactors
DIPiDIP-10244N
IntActiP09348, 2 interactors
STRINGi316385.ECDH10B_2031

Structurei

3D structure databases

ProteinModelPortaliP09348
SMRiP09348
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MotA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107RD7 Bacteria
COG1291 LUCA
HOGENOMiHOG000254701
InParanoidiP09348
KOiK02556
PhylomeDBiP09348

Family and domain databases

InterProiView protein in InterPro
IPR000540 Flag_MotA_CS
IPR022522 Flagellar_motor_stator_MotA
IPR002898 MotA_ExbB_proton_chnl
PANTHERiPTHR30433:SF4 PTHR30433:SF4, 1 hit
PfamiView protein in Pfam
PF01618 MotA_ExbB, 1 hit
TIGRFAMsiTIGR03818 MotA1, 1 hit
PROSITEiView protein in PROSITE
PS01307 MOTA, 1 hit

Sequencei

Sequence statusi: Complete.

P09348-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLILLGYLVV LGTVFGGYLM TGGSLGALYQ PAELVIIAGA GIGSFIVGNN
60 70 80 90 100
GKAIKGTLKA LPLLFRRSKY TKAMYMDLLA LLYRLMAKSR QMGMFSLERD
110 120 130 140 150
IENPRESEIF ASYPRILADS VMLDFIVDYL RLIISGHMNT FEIEALMDEE
160 170 180 190 200
IETHESEAEV PANSLALVGD SLPAFGIVAA VMGVVHALGS ADRPAAELGA
210 220 230 240 250
LIAHAMVGTF LGILLAYGFI SPLATVLRQK SAETSKMMQC VKVTLLSNLN
260 270 280 290
GYAPPIAVEF GRKTLYSSER PSFIELEEHV RAVKNPQQQT TTEEA
Length:295
Mass (Da):32,011
Last modified:July 1, 1989 - v1
Checksum:i355DC13EF63DD7D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA Translation: AAA24177.1
U00096 Genomic DNA Translation: AAC74960.1
AP009048 Genomic DNA Translation: BAA15711.1
PIRiA30279 QRECMA
RefSeqiNP_416404.1, NC_000913.3
WP_000906340.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74960; AAC74960; b1890
BAA15711; BAA15711; BAA15711
GeneIDi947564
KEGGiecj:JW1879
eco:b1890
PATRICifig|1411691.4.peg.357

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA Translation: AAA24177.1
U00096 Genomic DNA Translation: AAC74960.1
AP009048 Genomic DNA Translation: BAA15711.1
PIRiA30279 QRECMA
RefSeqiNP_416404.1, NC_000913.3
WP_000906340.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP09348
SMRiP09348
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262245, 486 interactors
DIPiDIP-10244N
IntActiP09348, 2 interactors
STRINGi316385.ECDH10B_2031

Protein family/group databases

TCDBi1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

Proteomic databases

PaxDbiP09348
PRIDEiP09348

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74960; AAC74960; b1890
BAA15711; BAA15711; BAA15711
GeneIDi947564
KEGGiecj:JW1879
eco:b1890
PATRICifig|1411691.4.peg.357

Organism-specific databases

EchoBASEiEB0596
EcoGeneiEG10601 motA

Phylogenomic databases

eggNOGiENOG4107RD7 Bacteria
COG1291 LUCA
HOGENOMiHOG000254701
InParanoidiP09348
KOiK02556
PhylomeDBiP09348

Enzyme and pathway databases

BioCyciEcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN

Miscellaneous databases

PROiPR:P09348

Family and domain databases

InterProiView protein in InterPro
IPR000540 Flag_MotA_CS
IPR022522 Flagellar_motor_stator_MotA
IPR002898 MotA_ExbB_proton_chnl
PANTHERiPTHR30433:SF4 PTHR30433:SF4, 1 hit
PfamiView protein in Pfam
PF01618 MotA_ExbB, 1 hit
TIGRFAMsiTIGR03818 MotA1, 1 hit
PROSITEiView protein in PROSITE
PS01307 MOTA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMOTA_ECOLI
AccessioniPrimary (citable) accession number: P09348
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 7, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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