UniProtKB - P09252 (DPOL_VZVD)
Protein
DNA polymerase catalytic subunit
Gene
ORF28
Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Functioni
Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication (By similarity).By similarity
Catalytic activityi
- EC:2.7.7.7
- Endonucleolytic cleavage to 5'-phosphomonoester. EC:3.1.26.4
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
- nucleotide binding Source: InterPro
- RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC
GO - Biological processi
- DNA replication Source: UniProtKB-KW
- viral DNA genome replication Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Endonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA replication, Viral DNA replication |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:ORF28 |
Organismi | Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3) |
Taxonomic identifieri | 10338 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
Other locations
- Host nucleus By similarity
Note: the protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs.By similarity
Other locations
- host cell nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host nucleusPathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL4859 |
DrugBanki | DB00787, Acyclovir DB13896, Talimogene laherparepvec |
DrugCentrali | P09252 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046525 | 1 – 1194 | DNA polymerase catalytic subunitAdd BLAST | 1194 |
Proteomic databases
PRIDEi | P09252 |
Interactioni
Subunit structurei
Forms a complex with the ssDNA-binding protein, the DNA polymerase processivity factor, and the alkaline exonuclease.
Interacts with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork (By similarity).
By similarityChemistry databases
BindingDBi | P09252 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DNA polymerase type-B family.Curated
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_C IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
i Sequence
Sequence statusi: Complete.
P09252-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIRTGFCNP FLTQASGIKY NPRTGRGSNR EFLHSYKTTM SSFQFLAPKC
60 70 80 90 100
LDEDVPMEER KGVHVGTLSR PPKVYCNGKE VPILDFRCSS PWPRRVNIWG
110 120 130 140 150
EIDFRGDKFD PRFNTFHVYD IVETTEAASN GDVSRFATAT RPLGTVITLL
160 170 180 190 200
GMSRCGKRVA VHVYGICQYF YINKAEVDTA CGIRSGSELS VLLAECLRSS
210 220 230 240 250
MITQNDATLN GDKNAFHGTS FKSASPESFR VEVIERTDVY YYDTQPCAFY
260 270 280 290 300
RVYSPSSKFT NYLCDNFHPE LKKYEGRVDA TTRFLMDNPG FVSFGWYQLK
310 320 330 340 350
PGVDGERVRV RPASRQLTLS DVEIDCMSDN LQAIPNDDSW PDYKLLCFDI
360 370 380 390 400
ECKSGGSNEL AFPDATHLED LVIQISCLLY SIPRQSLEHI LLFSLGSCDL
410 420 430 440 450
PQRYVQEMKD AGLPEPTVLE FDSEFELLIA FMTLVKQYAP EFATGYNIVN
460 470 480 490 500
FDWAFIMEKL NSIYSLKLDG YGSINRGGLF KIWDVGKSGF QRRSKVKING
510 520 530 540 550
LISLDMYAIA TEKLKLSSYK LDSVAREALN ESKRDLPYKD IPGYYASGPN
560 570 580 590 600
TRGIIGEYCI QDSALVGKLF FKYLPHLELS AVARLARITL TKAIYDGQQV
610 620 630 640 650
RIYTCLLGLA SSRGFILPDG GYPATFEYKD VIPDVGDVEE EMDEDESVSP
660 670 680 690 700
TGTSSGRNVG YKGARVFDPD TGFYIDPVVV LDFASLYPSI IQAHNLCFTT
710 720 730 740 750
LTLNFETVKR LNPSDYATFT VGGKRLFFVR SNVRESLLGV LLKDWLAMRK
760 770 780 790 800
AIRARIPGSS SDEAVLLDKQ QAAIKVVCNS VYGFTGVAQG FLPCLYVAAT
810 820 830 840 850
VTTIGRQMLL STRDYIHNNW AAFERFITAF PDIESSVLSQ KAYEVKVIYG
860 870 880 890 900
DTDSVFIRFK GVSVEGIAKI GEKMAHIIST ALFCPPIKLE CEKTFIKLLL
910 920 930 940 950
ITKKKYIGVI YGGKVLMKGV DLVRKNNCQF INDYARKLVE LLLYDDTVSR
960 970 980 990 1000
AAAEASCVSI AEWNRRAMPS GMAGFGRIIA DAHRQITSPK LDINKFVMTA
1010 1020 1030 1040 1050
ELSRPPSAYI NRRLAHLTVY YKLVMRQGQI PNVRERIPYV IVAPTDEVEA
1060 1070 1080 1090 1100
DAKSVALLRG DPLQNTAGKR CGEAKRKLII SDLAEDPIHV TSHGLSLNID
1110 1120 1130 1140 1150
YYFSHLIGTA SVTFKALFGN DTKLTERLLK RFIPETRVVN VKMLNRLQAA
1160 1170 1180 1190
GFVCIHAPCW DNKMNTEAEI TEEEQSHQIM RRVFCIPKAI LHQS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04370 Genomic DNA Translation: CAA27911.1 |
PIRi | B27214, DJBE28 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04370 Genomic DNA Translation: CAA27911.1 |
PIRi | B27214, DJBE28 |
3D structure databases
SMRi | P09252 |
ModBasei | Search... |
Chemistry databases
BindingDBi | P09252 |
ChEMBLi | CHEMBL4859 |
DrugBanki | DB00787, Acyclovir DB13896, Talimogene laherparepvec |
DrugCentrali | P09252 |
Proteomic databases
PRIDEi | P09252 |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_C IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOL_VZVD | |
Accessioni | P09252Primary (citable) accession number: P09252 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families