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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This isozyme may play a role in skeletal mineralization.By similarity

Miscellaneous

In most mammals there are four different isozymes: placental, germ cell, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotationBy similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediateBy similarity1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi332MagnesiumBy similarity1
Metal bindingi337Zinc 2By similarity1
Metal bindingi341Zinc 2; via tele nitrogenBy similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi379Zinc 1; via tele nitrogenBy similarity1
Metal bindingi454Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • pyrophosphatase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1 3474
ReactomeiR-MMU-163125 Post-translational modification: synthesis of GPI-anchored proteins
SABIO-RKiP09242

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1By similarity)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase 2
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:Alpl
Synonyms:Akp-2, Akp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:87983 Alpl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2660

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Add BLAST17
ChainiPRO_000002402518 – 501Alkaline phosphatase, tissue-nonspecific isozymeAdd BLAST484
PropeptideiPRO_0000024026502 – 524Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi230N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi430N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi489 ↔ 497By similarity
Lipidationi501GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP09242
PaxDbiP09242
PeptideAtlasiP09242
PRIDEiP09242

PTM databases

iPTMnetiP09242
PhosphoSitePlusiP09242
SwissPalmiP09242

Expressioni

Gene expression databases

BgeeiENSMUSG00000028766 Expressed in 232 organ(s), highest expression level in molar tooth
CleanExiMM_ALPL
ExpressionAtlasiP09242 baseline and differential
GenevisibleiP09242 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi198053, 3 interactors
IntActiP09242, 1 interactor
STRINGi10090.ENSMUSP00000030551

Structurei

3D structure databases

ProteinModelPortaliP09242
SMRiP09242
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP09242
KOiK01077
OMAiHYVWNRT
OrthoDBiEOG091G067H
TreeFamiTF323513

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P09242-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV
60 70 80 90 100
AKNVIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDQAIG KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
510 520
GSGSAPSPGA LLLPLAVLSL RTLF
Length:524
Mass (Da):57,514
Last modified:July 27, 2011 - v2
Checksum:i59D99110C60FA050
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q7P8E9Q7P8_MOUSE
Alkaline phosphatase
Alpl
216Annotation score:
B1AXF5B1AXF5_MOUSE
Alkaline phosphatase, tissue-nonspe...
Alpl
157Annotation score:
B1AXF6B1AXF6_MOUSE
Alkaline phosphatase, tissue-nonspe...
Alpl
98Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti521R → P in AAA39928 (PubMed:3478679).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02980 mRNA Translation: AAA39928.1
AK161780 mRNA Translation: BAE36569.1
AK167023 mRNA Translation: BAE39196.1
AL805954, AL807764 Genomic DNA Translation: CAM19406.1
AL807764, AL805954 Genomic DNA Translation: CAM23370.1
BC065175 mRNA Translation: AAH65175.1
M54798 mRNA Translation: AAA37217.1
CCDSiCCDS18821.1
RefSeqiNP_001274101.1, NM_001287172.1
NP_031457.2, NM_007431.3
XP_006538560.1, XM_006538497.3
XP_006538561.1, XM_006538498.3
XP_006538562.1, XM_006538499.3
XP_006538563.1, XM_006538500.2
XP_017175413.1, XM_017319924.1
UniGeneiMm.288186

Genome annotation databases

EnsembliENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766
GeneIDi11647
KEGGimmu:11647
UCSCiuc008vjr.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02980 mRNA Translation: AAA39928.1
AK161780 mRNA Translation: BAE36569.1
AK167023 mRNA Translation: BAE39196.1
AL805954, AL807764 Genomic DNA Translation: CAM19406.1
AL807764, AL805954 Genomic DNA Translation: CAM23370.1
BC065175 mRNA Translation: AAH65175.1
M54798 mRNA Translation: AAA37217.1
CCDSiCCDS18821.1
RefSeqiNP_001274101.1, NM_001287172.1
NP_031457.2, NM_007431.3
XP_006538560.1, XM_006538497.3
XP_006538561.1, XM_006538498.3
XP_006538562.1, XM_006538499.3
XP_006538563.1, XM_006538500.2
XP_017175413.1, XM_017319924.1
UniGeneiMm.288186

3D structure databases

ProteinModelPortaliP09242
SMRiP09242
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198053, 3 interactors
IntActiP09242, 1 interactor
STRINGi10090.ENSMUSP00000030551

Chemistry databases

ChEMBLiCHEMBL2660

PTM databases

iPTMnetiP09242
PhosphoSitePlusiP09242
SwissPalmiP09242

Proteomic databases

MaxQBiP09242
PaxDbiP09242
PeptideAtlasiP09242
PRIDEiP09242

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766
GeneIDi11647
KEGGimmu:11647
UCSCiuc008vjr.3 mouse

Organism-specific databases

CTDi249
MGIiMGI:87983 Alpl

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP09242
KOiK01077
OMAiHYVWNRT
OrthoDBiEOG091G067H
TreeFamiTF323513

Enzyme and pathway databases

BRENDAi3.1.3.1 3474
ReactomeiR-MMU-163125 Post-translational modification: synthesis of GPI-anchored proteins
SABIO-RKiP09242

Miscellaneous databases

PROiPR:P09242
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028766 Expressed in 232 organ(s), highest expression level in molar tooth
CleanExiMM_ALPL
ExpressionAtlasiP09242 baseline and differential
GenevisibleiP09242 MM

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPBT_MOUSE
AccessioniPrimary (citable) accession number: P09242
Secondary accession number(s): Q6P1B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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