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Protein

Protease 7

Gene

ompT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.1 Publication

Miscellaneous

Was originally classified as a serine protease, but it seems that OmpT could have a novel catalytic mechanism involving an Asp/His dyad and a pair of Asp.
Encoded by the cryptic lambdoid prophage DLP12.

Catalytic activityi

Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val.

Activity regulationi

Inhibited by zinc.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1031 Publication1
Active sitei1051 Publication1
Active sitei2301 Publication1
Active sitei2321 Publication1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB-KW
  • endopeptidase activity Source: EcoliWiki
  • serine-type endopeptidase activity Source: EcoliWiki

GO - Biological processi

  • proteolysis Source: EcoCyc

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10673-MONOMER
MetaCyc:EG10673-MONOMER
BRENDAi3.4.23.49 2026

Protein family/group databases

MEROPSiA26.001
TCDBi9.B.50.1.1 the outer membrane beta-barrel endoprotease, omptin (omptin) family

Names & Taxonomyi

Protein namesi
Recommended name:
Protease 7 (EC:3.4.23.49)
Alternative name(s):
Omptin
Outer membrane protein 3B
Protease A
Protease VII
Gene namesi
Name:ompT
Ordered Locus Names:b0565, JW0554
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10673 ompT

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 31PeriplasmicSequence analysisAdd BLAST11
Transmembranei32 – 41Beta strandedSequence analysis10
Topological domaini42 – 69ExtracellularSequence analysisAdd BLAST28
Transmembranei70 – 78Beta strandedSequence analysis9
Topological domaini79 – 83PeriplasmicSequence analysis5
Transmembranei84 – 92Beta strandedSequence analysis9
Topological domaini93 – 130ExtracellularSequence analysisAdd BLAST38
Transmembranei131 – 140Beta strandedSequence analysis10
Topological domaini141 – 145PeriplasmicSequence analysis5
Transmembranei146 – 156Beta strandedSequence analysisAdd BLAST11
Topological domaini157 – 197ExtracellularSequence analysisAdd BLAST41
Transmembranei198 – 209Beta strandedSequence analysisAdd BLAST12
Topological domaini210 – 211PeriplasmicSequence analysis2
Transmembranei212 – 221Beta strandedSequence analysis10
Topological domaini222 – 250ExtracellularSequence analysisAdd BLAST29
Transmembranei251 – 261Beta strandedSequence analysisAdd BLAST11
Topological domaini262 – 264PeriplasmicSequence analysis3
Transmembranei265 – 274Beta strandedSequence analysis10
Topological domaini275 – 306ExtracellularSequence analysisAdd BLAST32
Transmembranei307 – 316Beta strandedSequence analysis10
Topological domaini317PeriplasmicSequence analysis1

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103D → A: Loss of activity. 1 Publication1
Mutagenesisi105D → A: Loss of activity. 1 Publication1
Mutagenesisi230D → A: Loss of activity. 1 Publication1
Mutagenesisi236 – 237GK → KG: 70% of wild-type enzymatic activity. 2
Mutagenesisi237K → T: 40% of wild-type enzymatic activity. 1 Publication1
Mutagenesisi238R → L: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 204 PublicationsAdd BLAST20
ChainiPRO_000002581521 – 317Protease 7Add BLAST297

Proteomic databases

PaxDbiP09169
PRIDEiP09169

2D gel databases

SWISS-2DPAGEiP09169

Interactioni

Subunit structurei

Homopentamer.Curated

Protein-protein interaction databases

BioGridi4263520, 171 interactors
STRINGi316385.ECDH10B_0631

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP09169
SMRiP09169
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09169

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A26 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108NC2 Bacteria
COG4571 LUCA
HOGENOMiHOG000117799
KOiK01355
OMAiTANYRHE

Family and domain databases

InterProiView protein in InterPro
IPR020080 OM_adhesin/peptidase_omptin
IPR020079 Peptidase_A26_CS
IPR000036 Peptidase_A26_omptin
PfamiView protein in Pfam
PF01278 Omptin, 1 hit
PIRSFiPIRSF001522 Peptidase_A26, 1 hit
PRINTSiPR00482 OMPTIN
SUPFAMiSSF69917 SSF69917, 1 hit
PROSITEiView protein in PROSITE
PS00834 OMPTIN_1, 1 hit
PS00835 OMPTIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09169-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRAKLLGIVL TTPIAISSFA STETLSFTPD NINADISLGT LSGKTKERVY
60 70 80 90 100
LAEEGGRKVS QLDWKFNNAA IIKGAINWDL MPQISIGAAG WTTLGSRGGN
110 120 130 140 150
MVDQDWMDSS NPGTWTDESR HPDTQLNYAN EFDLNIKGWL LNEPNYRLGL
160 170 180 190 200
MAGYQESRYS FTARGGSYIY SSEEGFRDDI GSFPNGERAI GYKQRFKMPY
210 220 230 240 250
IGLTGSYRYE DFELGGTFKY SGWVESSDND EHYDPGKRIT YRSKVKDQNY
260 270 280 290 300
YSVAVNAGYY VTPNAKVYVE GAWNRVTNKK GNTSLYDHNN NTSDYSKNGA
310
GIENYNFITT AGLKYTF
Length:317
Mass (Da):35,562
Last modified:July 1, 1989 - v1
Checksum:iCA112FE7F6336F61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06903 Genomic DNA Translation: CAA30008.1
M23630 Genomic DNA Translation: AAA24430.1
U82598 Genomic DNA Translation: AAB40761.1
U00096 Genomic DNA Translation: AAC73666.1
AP009048 Genomic DNA Translation: BAA35199.2
PIRiA31387
RefSeqiNP_415097.1, NC_000913.3
WP_001201845.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73666; AAC73666; b0565
BAA35199; BAA35199; BAA35199
GeneIDi945185
KEGGiecj:JW0554
eco:b0565
PATRICifig|1411691.4.peg.1709

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06903 Genomic DNA Translation: CAA30008.1
M23630 Genomic DNA Translation: AAA24430.1
U82598 Genomic DNA Translation: AAB40761.1
U00096 Genomic DNA Translation: AAC73666.1
AP009048 Genomic DNA Translation: BAA35199.2
PIRiA31387
RefSeqiNP_415097.1, NC_000913.3
WP_001201845.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I78X-ray2.60A/B21-317[»]
ProteinModelPortaliP09169
SMRiP09169
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263520, 171 interactors
STRINGi316385.ECDH10B_0631

Protein family/group databases

MEROPSiA26.001
TCDBi9.B.50.1.1 the outer membrane beta-barrel endoprotease, omptin (omptin) family

2D gel databases

SWISS-2DPAGEiP09169

Proteomic databases

PaxDbiP09169
PRIDEiP09169

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73666; AAC73666; b0565
BAA35199; BAA35199; BAA35199
GeneIDi945185
KEGGiecj:JW0554
eco:b0565
PATRICifig|1411691.4.peg.1709

Organism-specific databases

EchoBASEiEB0667
EcoGeneiEG10673 ompT

Phylogenomic databases

eggNOGiENOG4108NC2 Bacteria
COG4571 LUCA
HOGENOMiHOG000117799
KOiK01355
OMAiTANYRHE

Enzyme and pathway databases

BioCyciEcoCyc:EG10673-MONOMER
MetaCyc:EG10673-MONOMER
BRENDAi3.4.23.49 2026

Miscellaneous databases

EvolutionaryTraceiP09169
PROiPR:P09169

Family and domain databases

InterProiView protein in InterPro
IPR020080 OM_adhesin/peptidase_omptin
IPR020079 Peptidase_A26_CS
IPR000036 Peptidase_A26_omptin
PfamiView protein in Pfam
PF01278 Omptin, 1 hit
PIRSFiPIRSF001522 Peptidase_A26, 1 hit
PRINTSiPR00482 OMPTIN
SUPFAMiSSF69917 SSF69917, 1 hit
PROSITEiView protein in PROSITE
PS00834 OMPTIN_1, 1 hit
PS00835 OMPTIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOMPT_ECOLI
AccessioniPrimary (citable) accession number: P09169
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 12, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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