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Protein

Galactose-1-phosphate uridylyltransferase

Gene

galT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Iron binding is not required for protein folding or enzyme activity.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose.3 Publications

Cofactori

Zn2+3 PublicationsNote: Binds 1 zinc ion per subunit. Zinc binding seems to play a structural role.3 Publications

Kineticsi

  1. KM=0.200 mM for uridine 5'-diphosphate glucose1 Publication
  2. KM=0.303 mM for galactose-1-phosphate1 Publication
  3. KM=0.121 mM for uridine 5'-diphosphate galactose1 Publication
  4. KM=0.157 mM for glucose-1-phosphate1 Publication
  1. Vmax=180 µmol/min/mg enzyme1 Publication

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52ZincCombined sources2 Publications1
Metal bindingi55ZincCombined sources2 Publications1
Binding sitei61UDP-alpha-D-glucose; via amide nitrogenCombined sources1 Publication1
Metal bindingi115ZincCombined sources2 Publications1
Binding sitei153UDP-alpha-D-glucoseCombined sources1 Publication1
Metal bindingi164ZincCombined sources2 Publications1
Active sitei166Tele-UMP-histidine intermediatePROSITE-ProRule annotation2 Publications1
Binding sitei168UDP-alpha-D-glucoseCombined sources1 Publication1
Metal bindingi182IronCombined sources2 Publications1
Metal bindingi281IronCombined sources2 Publications1
Metal bindingi296IronCombined sources2 Publications1
Metal bindingi298IronCombined sources2 Publications1
Binding sitei323UDP-alpha-D-glucoseCombined sources1 Publication1

GO - Molecular functioni

  • ferrous iron binding Source: EcoCyc
  • galactokinase activity Source: CACAO
  • UDP-glucose:hexose-1-phosphate uridylyltransferase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processCarbohydrate metabolism, Galactose metabolism
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GALACTURIDYLYLTRANS-MONOMER
MetaCyc:GALACTURIDYLYLTRANS-MONOMER
BRENDAi2.7.7.12 2026
SABIO-RKiP09148
UniPathwayi
UPA00214

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose-1-phosphate uridylyltransferase (EC:2.7.7.123 Publications)
Short name:
Gal-1-P uridylyltransferase
Alternative name(s):
UDP-glucose--hexose-1-phosphate uridylyltransferase
Gene namesi
Name:galT
Synonyms:galB
Ordered Locus Names:b0758, JW0741
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10366 galT

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52C → S: Decreases enzyme activity 3000-fold. 1 Publication1
Mutagenesisi55C → S: Decreases enzyme activity 600-fold. 1 Publication1
Mutagenesisi115H → N: Decreases enzyme activity by 98%. 1 Publication1
Mutagenesisi160C → S or A: Slight inhibition of enzymatic activity. 1 Publication1
Mutagenesisi161S → A: 7000-fold reduction in specific activity. 1 Publication1
Mutagenesisi164H → N: Decreases enzyme activity 10000-fold. 1 Publication1
Mutagenesisi166H → G: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi182E → A: Decreases enzyme activity by about 50%. Abolishes iron binding, but has no effect on zinc binding. 1 Publication1

Chemistry databases

DrugBankiDB01861 Uridine diphosphate glucose
DB03435 Uridine-5'-Diphosphate
DB03685 Uridine-5'-Monophosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001698941 – 348Galactose-1-phosphate uridylyltransferaseAdd BLAST348

Proteomic databases

PaxDbiP09148
PRIDEiP09148

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4261703, 397 interactors
DIPiDIP-9735N
IntActiP09148, 1 interactor
STRINGi316385.ECDH10B_0826

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP09148
SMRiP09148
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09148

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 31UDP-alpha-D-glucose binding; shared with dimeric partnerCombined sources1 Publication4
Regioni77 – 78UDP-alpha-D-glucose bindingCombined sources1 Publication2
Regioni159 – 161UDP-alpha-D-glucose bindingCombined sources1 Publication3
Regioni311 – 312UDP-alpha-D-glucose binding; shared with dimeric partnerCombined sources1 Publication2
Regioni316 – 317UDP-alpha-D-glucose binding; shared with dimeric partnerCombined sources1 Publication2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F0Z Bacteria
COG1085 LUCA
HOGENOMiHOG000230490
InParanoidiP09148
KOiK00965
PhylomeDBiP09148

Family and domain databases

CDDicd00608 GalT, 1 hit
Gene3Di3.30.428.10, 2 hits
InterProiView protein in InterPro
IPR001937 GalP_UDPtransf1
IPR019779 GalP_UDPtransf1_His-AS
IPR005850 GalP_Utransf_C
IPR005849 GalP_Utransf_N
IPR036265 HIT-like_sf
PANTHERiPTHR11943 PTHR11943, 1 hit
PfamiView protein in Pfam
PF02744 GalP_UDP_tr_C, 1 hit
PF01087 GalP_UDP_transf, 1 hit
PIRSFiPIRSF000808 GalT, 1 hit
SUPFAMiSSF54197 SSF54197, 2 hits
TIGRFAMsiTIGR00209 galT_1, 1 hit
PROSITEiView protein in PROSITE
PS00117 GAL_P_UDP_TRANSF_I, 1 hit

Sequencei

Sequence statusi: Complete.

P09148-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTQFNPVDHP HRRYNPLTGQ WILVSPHRAK RPWQGAQETP AKQVLPAHDP
60 70 80 90 100
DCFLCAGNVR VTGDKNPDYT GTYVFTNDFA ALMSDTPDAP ESHDPLMRCQ
110 120 130 140 150
SARGTSRVIC FSPDHSKTLP ELSVAALTEI VKTWQEQTAE LGKTYPWVQV
160 170 180 190 200
FENKGAAMGC SNPHPHGQIW ANSFLPNEAE REDRLQKEYF AEQKSPMLVD
210 220 230 240 250
YVQRELADGS RTVVETEHWL AVVPYWAAWP FETLLLPKAH VLRITDLTDA
260 270 280 290 300
QRSDLALALK KLTSRYDNLF QCSFPYSMGW HGAPFNGEEN QHWQLHAHFY
310 320 330 340
PPLLRSATVR KFMVGYEMLA ETQRDLTAEQ AAERLRAVSD IHFRESGV
Length:348
Mass (Da):39,646
Last modified:November 1, 1997 - v2
Checksum:i3D55D2CB38D8C9A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29 – 31AKR → LS in CAA29574 (PubMed:3022232).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06226 Genomic DNA Translation: CAA29574.1
U00096 Genomic DNA Translation: AAC73845.1
AP009048 Genomic DNA Translation: BAA35420.1
X02306 Genomic DNA Translation: CAA26171.1
PIRiS00722 XNECUD
RefSeqiNP_415279.1, NC_000913.3
WP_000191497.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73845; AAC73845; b0758
BAA35420; BAA35420; BAA35420
GeneIDi945357
KEGGiecj:JW0741
eco:b0758
PATRICifig|1411691.4.peg.1520

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06226 Genomic DNA Translation: CAA29574.1
U00096 Genomic DNA Translation: AAC73845.1
AP009048 Genomic DNA Translation: BAA35420.1
X02306 Genomic DNA Translation: CAA26171.1
PIRiS00722 XNECUD
RefSeqiNP_415279.1, NC_000913.3
WP_000191497.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUPX-ray1.80A/B/C/D1-348[»]
1GUQX-ray1.80A/B/C/D1-348[»]
1HXPX-ray1.80A/B1-348[»]
1HXQX-ray1.86A/B1-348[»]
ProteinModelPortaliP09148
SMRiP09148
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261703, 397 interactors
DIPiDIP-9735N
IntActiP09148, 1 interactor
STRINGi316385.ECDH10B_0826

Chemistry databases

DrugBankiDB01861 Uridine diphosphate glucose
DB03435 Uridine-5'-Diphosphate
DB03685 Uridine-5'-Monophosphate

Proteomic databases

PaxDbiP09148
PRIDEiP09148

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73845; AAC73845; b0758
BAA35420; BAA35420; BAA35420
GeneIDi945357
KEGGiecj:JW0741
eco:b0758
PATRICifig|1411691.4.peg.1520

Organism-specific databases

EchoBASEiEB0361
EcoGeneiEG10366 galT

Phylogenomic databases

eggNOGiENOG4105F0Z Bacteria
COG1085 LUCA
HOGENOMiHOG000230490
InParanoidiP09148
KOiK00965
PhylomeDBiP09148

Enzyme and pathway databases

UniPathwayi
UPA00214

BioCyciEcoCyc:GALACTURIDYLYLTRANS-MONOMER
MetaCyc:GALACTURIDYLYLTRANS-MONOMER
BRENDAi2.7.7.12 2026
SABIO-RKiP09148

Miscellaneous databases

EvolutionaryTraceiP09148
PROiPR:P09148

Family and domain databases

CDDicd00608 GalT, 1 hit
Gene3Di3.30.428.10, 2 hits
InterProiView protein in InterPro
IPR001937 GalP_UDPtransf1
IPR019779 GalP_UDPtransf1_His-AS
IPR005850 GalP_Utransf_C
IPR005849 GalP_Utransf_N
IPR036265 HIT-like_sf
PANTHERiPTHR11943 PTHR11943, 1 hit
PfamiView protein in Pfam
PF02744 GalP_UDP_tr_C, 1 hit
PF01087 GalP_UDP_transf, 1 hit
PIRSFiPIRSF000808 GalT, 1 hit
SUPFAMiSSF54197 SSF54197, 2 hits
TIGRFAMsiTIGR00209 galT_1, 1 hit
PROSITEiView protein in PROSITE
PS00117 GAL_P_UDP_TRANSF_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGAL7_ECOLI
AccessioniPrimary (citable) accession number: P09148
Secondary accession number(s): P78270
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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