UniProtKB - P09148 (GAL7_ECOLI)
Protein
Galactose-1-phosphate uridylyltransferase
Gene
galT
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
Iron binding is not required for protein folding or enzyme activity.1 Publication
Catalytic activityi
- α-D-galactose 1-phosphate + UDP-α-D-glucose = α-D-glucose 1-phosphate + UDP-α-D-galactose3 PublicationsEC:2.7.7.123 Publications
Cofactori
Zn2+3 PublicationsNote: Binds 1 zinc ion per subunit. Zinc binding seems to play a structural role.3 Publications
Kineticsi
- KM=0.200 mM for uridine 5'-diphosphate glucose1 Publication
- KM=0.303 mM for galactose-1-phosphate1 Publication
- KM=0.121 mM for uridine 5'-diphosphate galactose1 Publication
- KM=0.157 mM for glucose-1-phosphate1 Publication
- Vmax=180 µmol/min/mg enzyme1 Publication
: galactose metabolism Pathwayi
This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 52 | ZincCombined sources2 Publications | 1 | |
Metal bindingi | 55 | ZincCombined sources2 Publications | 1 | |
Binding sitei | 61 | UDP-alpha-D-glucose; via amide nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 115 | ZincCombined sources2 Publications | 1 | |
Binding sitei | 153 | UDP-alpha-D-glucoseCombined sources1 Publication | 1 | |
Metal bindingi | 164 | ZincCombined sources2 Publications | 1 | |
Active sitei | 166 | Tele-UMP-histidine intermediatePROSITE-ProRule annotation2 Publications | 1 | |
Binding sitei | 168 | UDP-alpha-D-glucoseCombined sources1 Publication | 1 | |
Metal bindingi | 182 | IronCombined sources2 Publications | 1 | |
Metal bindingi | 281 | IronCombined sources2 Publications | 1 | |
Metal bindingi | 296 | IronCombined sources2 Publications | 1 | |
Metal bindingi | 298 | IronCombined sources2 Publications | 1 | |
Binding sitei | 323 | UDP-alpha-D-glucoseCombined sources1 Publication | 1 |
GO - Molecular functioni
- ferrous iron binding Source: EcoCyc
- galactokinase activity Source: CACAO
- UDP-glucose:hexose-1-phosphate uridylyltransferase activity Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- galactose catabolic process via UDP-galactose Source: EcoCyc
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Galactose metabolism |
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:GALACTURIDYLYLTRANS-MONOMER MetaCyc:GALACTURIDYLYLTRANS-MONOMER |
BRENDAi | 2.7.7.12, 2026 |
SABIO-RKi | P09148 |
UniPathwayi | UPA00214 |
Names & Taxonomyi
Protein namesi | Recommended name: Galactose-1-phosphate uridylyltransferase (EC:2.7.7.123 Publications)Short name: Gal-1-P uridylyltransferase Alternative name(s): UDP-glucose--hexose-1-phosphate uridylyltransferase |
Gene namesi | Name:galT Synonyms:galB Ordered Locus Names:b0758, JW0741 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | C → S: Decreases enzyme activity 3000-fold. 1 Publication | 1 | |
Mutagenesisi | 55 | C → S: Decreases enzyme activity 600-fold. 1 Publication | 1 | |
Mutagenesisi | 115 | H → N: Decreases enzyme activity by 98%. 1 Publication | 1 | |
Mutagenesisi | 160 | C → S or A: Slight inhibition of enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 161 | S → A: 7000-fold reduction in specific activity. 1 Publication | 1 | |
Mutagenesisi | 164 | H → N: Decreases enzyme activity 10000-fold. 1 Publication | 1 | |
Mutagenesisi | 166 | H → G: Abolishes enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 182 | E → A: Decreases enzyme activity by about 50%. Abolishes iron binding, but has no effect on zinc binding. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB01861, Uridine diphosphate glucose DB03685, Uridine monophosphate DB03435, Uridine-5'-Diphosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169894 | 1 – 348 | Galactose-1-phosphate uridylyltransferaseAdd BLAST | 348 |
Proteomic databases
jPOSTi | P09148 |
PaxDbi | P09148 |
PRIDEi | P09148 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 4261703, 397 interactors |
DIPi | DIP-9735N |
IntActi | P09148, 1 interactor |
STRINGi | 511145.b0758 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09148 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09148 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 28 – 31 | UDP-alpha-D-glucose binding; shared with dimeric partnerCombined sources1 Publication | 4 | |
Regioni | 77 – 78 | UDP-alpha-D-glucose bindingCombined sources1 Publication | 2 | |
Regioni | 159 – 161 | UDP-alpha-D-glucose bindingCombined sources1 Publication | 3 | |
Regioni | 311 – 312 | UDP-alpha-D-glucose binding; shared with dimeric partnerCombined sources1 Publication | 2 | |
Regioni | 316 – 317 | UDP-alpha-D-glucose binding; shared with dimeric partnerCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.Curated
Phylogenomic databases
eggNOGi | COG1085, Bacteria |
HOGENOMi | CLU_029960_0_0_6 |
InParanoidi | P09148 |
PhylomeDBi | P09148 |
Family and domain databases
CDDi | cd00608, GalT, 1 hit |
Gene3Di | 3.30.428.10, 2 hits |
InterProi | View protein in InterPro IPR001937, GalP_UDPtransf1 IPR019779, GalP_UDPtransf1_His-AS IPR005850, GalP_Utransf_C IPR005849, GalP_Utransf_N IPR036265, HIT-like_sf |
PANTHERi | PTHR11943, PTHR11943, 1 hit |
Pfami | View protein in Pfam PF02744, GalP_UDP_tr_C, 1 hit PF01087, GalP_UDP_transf, 1 hit |
PIRSFi | PIRSF000808, GalT, 1 hit |
SUPFAMi | SSF54197, SSF54197, 2 hits |
TIGRFAMsi | TIGR00209, galT_1, 1 hit |
PROSITEi | View protein in PROSITE PS00117, GAL_P_UDP_TRANSF_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P09148-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQFNPVDHP HRRYNPLTGQ WILVSPHRAK RPWQGAQETP AKQVLPAHDP
60 70 80 90 100
DCFLCAGNVR VTGDKNPDYT GTYVFTNDFA ALMSDTPDAP ESHDPLMRCQ
110 120 130 140 150
SARGTSRVIC FSPDHSKTLP ELSVAALTEI VKTWQEQTAE LGKTYPWVQV
160 170 180 190 200
FENKGAAMGC SNPHPHGQIW ANSFLPNEAE REDRLQKEYF AEQKSPMLVD
210 220 230 240 250
YVQRELADGS RTVVETEHWL AVVPYWAAWP FETLLLPKAH VLRITDLTDA
260 270 280 290 300
QRSDLALALK KLTSRYDNLF QCSFPYSMGW HGAPFNGEEN QHWQLHAHFY
310 320 330 340
PPLLRSATVR KFMVGYEMLA ETQRDLTAEQ AAERLRAVSD IHFRESGV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 – 31 | AKR → LS in CAA29574 (PubMed:3022232).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06226 Genomic DNA Translation: CAA29574.1 U00096 Genomic DNA Translation: AAC73845.1 AP009048 Genomic DNA Translation: BAA35420.1 X02306 Genomic DNA Translation: CAA26171.1 |
PIRi | S00722, XNECUD |
RefSeqi | NP_415279.1, NC_000913.3 WP_000191497.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73845; AAC73845; b0758 BAA35420; BAA35420; BAA35420 |
GeneIDi | 945357 |
KEGGi | ecj:JW0741 eco:b0758 |
PATRICi | fig|1411691.4.peg.1520 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06226 Genomic DNA Translation: CAA29574.1 U00096 Genomic DNA Translation: AAC73845.1 AP009048 Genomic DNA Translation: BAA35420.1 X02306 Genomic DNA Translation: CAA26171.1 |
PIRi | S00722, XNECUD |
RefSeqi | NP_415279.1, NC_000913.3 WP_000191497.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GUP | X-ray | 1.80 | A/B/C/D | 1-348 | [»] | |
1GUQ | X-ray | 1.80 | A/B/C/D | 1-348 | [»] | |
1HXP | X-ray | 1.80 | A/B | 1-348 | [»] | |
1HXQ | X-ray | 1.86 | A/B | 1-348 | [»] | |
SMRi | P09148 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261703, 397 interactors |
DIPi | DIP-9735N |
IntActi | P09148, 1 interactor |
STRINGi | 511145.b0758 |
Chemistry databases
DrugBanki | DB01861, Uridine diphosphate glucose DB03685, Uridine monophosphate DB03435, Uridine-5'-Diphosphate |
Proteomic databases
jPOSTi | P09148 |
PaxDbi | P09148 |
PRIDEi | P09148 |
Genome annotation databases
EnsemblBacteriai | AAC73845; AAC73845; b0758 BAA35420; BAA35420; BAA35420 |
GeneIDi | 945357 |
KEGGi | ecj:JW0741 eco:b0758 |
PATRICi | fig|1411691.4.peg.1520 |
Organism-specific databases
EchoBASEi | EB0361 |
Phylogenomic databases
eggNOGi | COG1085, Bacteria |
HOGENOMi | CLU_029960_0_0_6 |
InParanoidi | P09148 |
PhylomeDBi | P09148 |
Enzyme and pathway databases
UniPathwayi | UPA00214 |
BioCyci | EcoCyc:GALACTURIDYLYLTRANS-MONOMER MetaCyc:GALACTURIDYLYLTRANS-MONOMER |
BRENDAi | 2.7.7.12, 2026 |
SABIO-RKi | P09148 |
Miscellaneous databases
EvolutionaryTracei | P09148 |
PROi | PR:P09148 |
Family and domain databases
CDDi | cd00608, GalT, 1 hit |
Gene3Di | 3.30.428.10, 2 hits |
InterProi | View protein in InterPro IPR001937, GalP_UDPtransf1 IPR019779, GalP_UDPtransf1_His-AS IPR005850, GalP_Utransf_C IPR005849, GalP_Utransf_N IPR036265, HIT-like_sf |
PANTHERi | PTHR11943, PTHR11943, 1 hit |
Pfami | View protein in Pfam PF02744, GalP_UDP_tr_C, 1 hit PF01087, GalP_UDP_transf, 1 hit |
PIRSFi | PIRSF000808, GalT, 1 hit |
SUPFAMi | SSF54197, SSF54197, 2 hits |
TIGRFAMsi | TIGR00209, galT_1, 1 hit |
PROSITEi | View protein in PROSITE PS00117, GAL_P_UDP_TRANSF_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GAL7_ECOLI | |
Accessioni | P09148Primary (citable) accession number: P09148 Secondary accession number(s): P78270 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families