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Entry version 128 (02 Dec 2020)
Sequence version 4 (10 May 2005)
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Protein

Coupling protein TraD

Gene

traD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Couples the transferosome to a type IV secretion system. Probably forms a pore through which single-stranded plasmid DNA is transferred to the secretion system. The last 37 residues are important for determining plasmid specificity and transfer efficiency, with additional specificity conferred by the TraD-TraM pair.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi192 – 199ATPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • unidirectional conjugation Source: CACAO

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processConjugation
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Coupling protein TraD
Alternative name(s):
DNA transport protein TraD
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:traD
Ordered Locus Names:ECOK12F102
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid F14 Publications
Plasmid p1658/971 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 27Cytoplasmic1 PublicationAdd BLAST27
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei28 – 47HelicalCuratedAdd BLAST20
Topological domaini48 – 104Periplasmic1 PublicationAdd BLAST57
Transmembranei105 – 130HelicalCuratedAdd BLAST26
Topological domaini131 – 717Cytoplasmic1 PublicationAdd BLAST587

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi681 – 717WQQEN…PGDDF → CNRKIIRTSSSRCSVVKR: 1000-fold increase in the transfer frequency of plasmids R388 and RSF1010, a 10,000-fold reduction in F plasmid transfer frequency. 1 PublicationAdd BLAST37
Mutagenesisi710 – 717Missing : Loss of interaction with TraM, 1000-fold decrease in conjugation. 1 Publication8
Mutagenesisi712E → K: No change in conjugation efficiency. Partially rescues a TraM K-99 mutation. 1 Publication1
Mutagenesisi715D → K: 10-fold decrease in conjugation efficiency. 1 Publication1
Mutagenesisi717F → A: 5000-fold decrease in conjugation efficiency. 1 Publication1
Mutagenesisi717F → FG: 1000-fold decrease in conjugation efficiency. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000684481 – 717Coupling protein TraDAdd BLAST717

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P09130

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with relaxosome component TraM. May form a hexamer in the membrane.

3 Publications

Protein-protein interaction databases

Database of interacting proteins

More...
DIPi
DIP-27652N

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P09130

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P09130

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TrwB coupling protein family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01127, TrwB, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR027417, P-loop_NTPase
IPR014128, T4SS_TraD
IPR019476, T4SS_TraD_DNA-bd
IPR022585, TraD_N

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF12615, TraD_N, 1 hit
PF10412, TrwB_AAD_bind, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540, SSF52540, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02759, TraD_Ftype, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P09130-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSFNAKDMTQ GGQIASMRIR MFSQIANIML YCLFIFFWIL VGLVLWIKIS
60 70 80 90 100
WQTFVNGCIY WWCTTLEGMR DLIKSQPVYE IQYYGKTFRM NAAQVLHDKY
110 120 130 140 150
MIWCSEQLWS AFVLAAVVAL VICLITFFVV SWILGRQGKQ QSENEVTGGR
160 170 180 190 200
QLTDNPKDVA RMLKKDGKDS DIRIGDLPII RDSEIQNFCL HGTVGAGKSE
210 220 230 240 250
VIRRLANYAR QRGDMVVIYD RSGEFVKSYY DPSIDKILNP LDARCAAWDL
260 270 280 290 300
WKECLTQPDF DNTANTLIPM GTKEDPFWQG SGRTIFAEAA YLMRNDPNRS
310 320 330 340 350
YSKLVDTLLS IKIEKLRTYL RNSPAANLVE EKIEKTAISI RAVLTNYVKA
360 370 380 390 400
IRYLQGIEHN GEPFTIRDWM RGVREDQKNG WLFISSNADT HASLKPVISM
410 420 430 440 450
WLSIAIRGLL AMGENRNRRV WFFCDELPTL HKLPDLVEIL PEARKFGGCY
460 470 480 490 500
VFGIQSYAQL EDIYGEKAAA SLFDVMNTRA FFRSPSHKIA EFAAGEIGEK
510 520 530 540 550
EHLKASEQYS YGADPVRDGV STGKDMERQT LVSYSDIQSL PDLTCYVTLP
560 570 580 590 600
GPYPAVKLSL KYQTRPKVAP EFIPRDINPE MENRLSAVLA AREAEGRQMA
610 620 630 640 650
SLFEPDVPEV VSGEDVTQAE QPQQPVSPAI NDKKSDSGVN VPAGGIEQEL
660 670 680 690 700
KMKPEEEMEQ QLPPGISESG EVVDMAAYEA WQQENHPDIQ QQMQRREEVN
710
INVHRERGED VEPGDDF
Length:717
Mass (Da):81,489
Last modified:May 10, 2005 - v4
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iEA43168766F3E6F3
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAC44181 differs from that shown. Reason: Erroneous termination. Extended C-terminus.Curated
The sequence CAA30013 differs from that shown. Reason: Frameshift.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti19 – 62IRMFS…CIYWW → YPHVQPDRQYHALLPVYFFL DTRWSGFMDKISWTDVVNGL FTV in AAA83928 (PubMed:2680768).CuratedAdd BLAST44
Sequence conflicti190L → R in AAA83928 (PubMed:2680768).Curated1
Sequence conflicti269 – 270PM → RD in AAA83928 (PubMed:2680768).Curated2
Sequence conflicti321 – 325RNSPA → VIHRQ in AAA83928 (PubMed:2680768).Curated5

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti396P → L in traD39; temperature-sensitive. 1 Publication1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M29254 Genomic DNA Translation: AAA83928.1
U01159 Genomic DNA Translation: AAC44181.2 Different termination.
AJ011707 Genomic DNA Translation: CAA09749.1
AP001918 Genomic DNA Translation: BAA97972.1
AF550679 Genomic DNA Translation: AAO49544.1
X06915 Genomic DNA Translation: CAA30013.1 Frameshift.
X57428 Genomic DNA Translation: CAA40674.1
X57431 Genomic DNA Translation: CAA40678.1
M54796 Genomic DNA Translation: AAA98083.1

Protein sequence database of the Protein Information Resource

More...
PIRi
JS0293, BVECAD

NCBI Reference Sequences

More...
RefSeqi
NP_061481.1, NC_002483.1
NP_862947.1, NC_004998.1
WP_000009350.1, NZ_CP014273.1
YP_009060132.1, NC_024956.1
YP_009062862.1, NC_025014.1
YP_009068320.1, NC_025139.1
YP_009070586.1, NC_025175.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1263585
1446510
20466888
20468912
20491450
20492654

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|83333.107.peg.610

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29254 Genomic DNA Translation: AAA83928.1
U01159 Genomic DNA Translation: AAC44181.2 Different termination.
AJ011707 Genomic DNA Translation: CAA09749.1
AP001918 Genomic DNA Translation: BAA97972.1
AF550679 Genomic DNA Translation: AAO49544.1
X06915 Genomic DNA Translation: CAA30013.1 Frameshift.
X57428 Genomic DNA Translation: CAA40674.1
X57431 Genomic DNA Translation: CAA40678.1
M54796 Genomic DNA Translation: AAA98083.1
PIRiJS0293, BVECAD
RefSeqiNP_061481.1, NC_002483.1
NP_862947.1, NC_004998.1
WP_000009350.1, NZ_CP014273.1
YP_009060132.1, NC_024956.1
YP_009062862.1, NC_025014.1
YP_009068320.1, NC_025139.1
YP_009070586.1, NC_025175.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D8AX-ray2.55S/T/U/V/W/X/Y/Z708-717[»]
SMRiP09130
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

DIPiDIP-27652N

Proteomic databases

PRIDEiP09130

Genome annotation databases

GeneIDi1263585
1446510
20466888
20468912
20491450
20492654
PATRICifig|83333.107.peg.610

Miscellaneous databases

EvolutionaryTraceiP09130

Protein Ontology

More...
PROi
PR:P09130

Family and domain databases

CDDicd01127, TrwB, 1 hit
InterProiView protein in InterPro
IPR027417, P-loop_NTPase
IPR014128, T4SS_TraD
IPR019476, T4SS_TraD_DNA-bd
IPR022585, TraD_N
PfamiView protein in Pfam
PF12615, TraD_N, 1 hit
PF10412, TrwB_AAD_bind, 1 hit
SUPFAMiSSF52540, SSF52540, 1 hit
TIGRFAMsiTIGR02759, TraD_Ftype, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTRAD1_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P09130
Secondary accession number(s): O87742
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 10, 2005
Last modified: December 2, 2020
This is version 128 of the entry and version 4 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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