UniProtKB - P09126 (HEM4_ECOLI)
Protein
Uroporphyrinogen-III synthase
Gene
hemD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity
Catalytic activityi
- EC:4.2.1.75
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Delta-aminolevulinic acid dehydratase (hemB), Delta-aminolevulinic acid dehydratase (hemB)
- Porphobilinogen deaminase (hemC), Porphobilinogen deaminase (hemC)
- Uroporphyrinogen-III synthase (hemD), Uroporphyrinogen-III synthase (hemD)
- Uroporphyrinogen decarboxylase (hemE), Uroporphyrinogen decarboxylase (hemE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
GO - Molecular functioni
- uroporphyrinogen-III synthase activity Source: EcoCyc
GO - Biological processi
- protoporphyrinogen IX biosynthetic process Source: EcoCyc
- uroporphyrinogen III biosynthetic process Source: InterPro
Keywordsi
Molecular function | Lyase |
Biological process | Porphyrin biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:UROGENIIISYN-MONOMER MetaCyc:UROGENIIISYN-MONOMER |
UniPathwayi | UPA00251;UER00320 |
Names & Taxonomyi
Protein namesi | Recommended name: Uroporphyrinogen-III synthase (EC:4.2.1.75)Short name: UROS Alternative name(s): Hydroxymethylbilane hydrolyase [cyclizing] Uroporphyrinogen-III cosynthase |
Gene namesi | Name:hemD Ordered Locus Names:b3804, JW3776 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135243 | 1 – 246 | Uroporphyrinogen-III synthaseAdd BLAST | 246 |
Proteomic databases
jPOSTi | P09126 |
PaxDbi | P09126 |
PRIDEi | P09126 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4262609, 4 interactors |
IntActi | P09126, 3 interactors |
STRINGi | 511145.b3804 |
Family & Domainsi
Sequence similaritiesi
Belongs to the uroporphyrinogen-III synthase family.Curated
Phylogenomic databases
eggNOGi | COG1587, Bacteria |
HOGENOMi | CLU_011276_9_4_6 |
InParanoidi | P09126 |
PhylomeDBi | P09126 |
Family and domain databases
CDDi | cd06578, HemD, 1 hit |
Gene3Di | 3.40.50.10090, 2 hits |
InterProi | View protein in InterPro IPR036108, 4pyrrol_syn_uPrphyn_synt_sf IPR003754, 4pyrrol_synth_uPrphyn_synth IPR039793, UROS/Hem4 |
PANTHERi | PTHR38042, PTHR38042, 1 hit |
Pfami | View protein in Pfam PF02602, HEM4, 1 hit |
SUPFAMi | SSF69618, SSF69618, 1 hit |
i Sequence
Sequence statusi: Complete.
P09126-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL
60 70 80 90 100
GESDLLFALS QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG
110 120 130 140 150
QKILYPQDRE ISEVLLQLPE LQNIAGKRAL ILRGNGGREL IGDTLTARGA
160 170 180 190 200
EVTFCECYQR CAIHYDGAEE AMRWQAREVT MVVVTSGEML QQLWSLIPQW
210 220 230 240
YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 146 | T → A in AAA23956 (PubMed:3040684).Curated | 1 | |
Sequence conflicti | 236 | A → R (PubMed:3320969).Curated | 1 | |
Sequence conflicti | 236 | A → R (PubMed:3277628).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12614 Genomic DNA Translation: CAA31133.1 Y00883 Genomic DNA Translation: CAA68776.1 M17360 Genomic DNA Translation: AAA23956.1 M87049 Genomic DNA Translation: AAA67600.1 U00096 Genomic DNA Translation: AAC76807.1 AP009048 Genomic DNA Translation: BAE77497.1 |
PIRi | E65184 |
RefSeqi | NP_418248.1, NC_000913.3 WP_000026046.1, NZ_SSZK01000025.1 |
Genome annotation databases
EnsemblBacteriai | AAC76807; AAC76807; b3804 BAE77497; BAE77497; BAE77497 |
GeneIDi | 948587 |
KEGGi | ecj:JW3776 eco:b3804 |
PATRICi | fig|1411691.4.peg.2904 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12614 Genomic DNA Translation: CAA31133.1 Y00883 Genomic DNA Translation: CAA68776.1 M17360 Genomic DNA Translation: AAA23956.1 M87049 Genomic DNA Translation: AAA67600.1 U00096 Genomic DNA Translation: AAC76807.1 AP009048 Genomic DNA Translation: BAE77497.1 |
PIRi | E65184 |
RefSeqi | NP_418248.1, NC_000913.3 WP_000026046.1, NZ_SSZK01000025.1 |
3D structure databases
SMRi | P09126 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262609, 4 interactors |
IntActi | P09126, 3 interactors |
STRINGi | 511145.b3804 |
Proteomic databases
jPOSTi | P09126 |
PaxDbi | P09126 |
PRIDEi | P09126 |
Protocols and materials databases
DNASUi | 948587 |
Genome annotation databases
EnsemblBacteriai | AAC76807; AAC76807; b3804 BAE77497; BAE77497; BAE77497 |
GeneIDi | 948587 |
KEGGi | ecj:JW3776 eco:b3804 |
PATRICi | fig|1411691.4.peg.2904 |
Organism-specific databases
EchoBASEi | EB0425 |
Phylogenomic databases
eggNOGi | COG1587, Bacteria |
HOGENOMi | CLU_011276_9_4_6 |
InParanoidi | P09126 |
PhylomeDBi | P09126 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00320 |
BioCyci | EcoCyc:UROGENIIISYN-MONOMER MetaCyc:UROGENIIISYN-MONOMER |
Miscellaneous databases
PROi | PR:P09126 |
Family and domain databases
CDDi | cd06578, HemD, 1 hit |
Gene3Di | 3.40.50.10090, 2 hits |
InterProi | View protein in InterPro IPR036108, 4pyrrol_syn_uPrphyn_synt_sf IPR003754, 4pyrrol_synth_uPrphyn_synth IPR039793, UROS/Hem4 |
PANTHERi | PTHR38042, PTHR38042, 1 hit |
Pfami | View protein in Pfam PF02602, HEM4, 1 hit |
SUPFAMi | SSF69618, SSF69618, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HEM4_ECOLI | |
Accessioni | P09126Primary (citable) accession number: P09126 Secondary accession number(s): Q2M8A9, Q47250 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families