hemD

Functionⁱ

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Catalytic activityⁱ

Hydroxymethylbilane = uroporphyrinogen III + H₂O.

Pathwayⁱ: protoporphyrin-IX biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

Delta-aminolevulinic acid dehydratase (hemB)
Porphobilinogen deaminase (hemC)
Uroporphyrinogen-III synthase (hemD)
Uroporphyrinogen decarboxylase (hemE)

This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functionⁱ

uroporphyrinogen-III synthase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 2557837

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protoporphyrinogen IX biosynthetic process
Source: EcoCyc
Inferred from direct assayⁱ
- 8224882

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Porphyrin biosynthesis

Enzyme and pathway databases

BioCycⁱ	EcoCyc:UROGENIIISYN-MONOMER MetaCyc:UROGENIIISYN-MONOMER
UniPathwayⁱ	UPA00251; UER00320

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Uroporphyrinogen-III synthase (EC:4.2.1.75) Short name: UROS Alternative name(s): Hydroxymethylbilane hydrolyase [cyclizing] Uroporphyrinogen-III cosynthase
Gene namesⁱ	Name:hemD Ordered Locus Names:b3804, JW3776
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10430 hemD

EcoGeneⁱ

EG10430 hemD

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000135243	1 – 246	Uroporphyrinogen-III synthaseAdd BLAST		246

Proteomic databases

PaxDbⁱ	P09126
PRIDEⁱ	P09126

Interactionⁱ

Subunit structureⁱ

Monomer.

Protein-protein interaction databases

BioGridⁱ	4262609, 4 interactors
IntActⁱ	P09126, 3 interactors
STRINGⁱ	316385.ECDH10B_3995

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P09126
SMRⁱ	P09126
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the uroporphyrinogen-III synthase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG41076E6 Bacteria COG1587 LUCA
HOGENOMⁱ	HOG000247993
InParanoidⁱ	P09126
KEGG Orthology (KO) More...KOⁱ	K01719
OMAⁱ	TRPAEDC
PhylomeDBⁱ	P09126

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06578 HemD, 1 hit
InterProⁱ	View protein in InterPro IPR036108 4pyrrol_syn_uPrphyn_synt_sf IPR003754 4pyrrol_synth_uPrphyn_synth
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02602 HEM4, 1 hit
SUPFAMⁱ	SSF69618 SSF69618, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P09126-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL 
        60         70         80         90        100
GESDLLFALS QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG 
       110        120        130        140        150
QKILYPQDRE ISEVLLQLPE LQNIAGKRAL ILRGNGGREL IGDTLTARGA 
       160        170        180        190        200
EVTFCECYQR CAIHYDGAEE AMRWQAREVT MVVVTSGEML QQLWSLIPQW 
       210        220        230        240 
YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ

Length:246

Mass (Da):27,798

Last modified:July 1, 1989 - v1

Checksum:ⁱ0BF7453C72395A0C

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	146	T → A in AAA23956 (PubMed:3040684).Curated	1
Sequence conflictⁱ	236	A → R (PubMed:3320969).Curated	1
Sequence conflictⁱ	236	A → R (PubMed:3277628).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X12614 Genomic DNA Translation: CAA31133.1 Y00883 Genomic DNA Translation: CAA68776.1 M17360 Genomic DNA Translation: AAA23956.1 M87049 Genomic DNA Translation: AAA67600.1 U00096 Genomic DNA Translation: AAC76807.1 AP009048 Genomic DNA Translation: BAE77497.1
PIRⁱ	E65184
NCBI Reference Sequences More...RefSeqⁱ	NP_418248.1, NC_000913.3 WP_000026046.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76807; AAC76807; b3804 BAE77497; BAE77497; BAE77497
GeneIDⁱ	948587
KEGGⁱ	ecj:JW3776 eco:b3804
PATRICⁱ	fig\|1411691.4.peg.2904

Protein	Similar proteins		Species	Score	Length
P09126	Uroporphyrinogen III methyltransferase		ECOLX		246
Uroporphyrinogen III synthase		Escherichia coli G3/10		246
Uroporphyrinogen-III synthase		Escherichia coli KTE108		246
Uroporphyrinogen-III synthase HemD family protein		Escherichia coli 1-110-08_S3_C1		246
Uroporphyrinogen-III synthase HemD family protein		Escherichia coli DEC6A		246
+9

Protein	Similar proteins		Species	Score	Length
P09126	Uroporphyrinogen III methyltransferase		ECOLX		246
Uroporphyrinogen III synthase		Escherichia coli G3/10		246
Uroporphyrinogen-III synthase		Escherichia coli KTE108		246
Uroporphyrinogen-III synthase HemD family protein		Escherichia coli 1-110-08_S3_C1		246
Uroporphyrinogen-III synthase HemD family protein		Escherichia coli DEC6A		246
+405

Protein	Similar proteins		Species	Score	Length
P09126	Uroporphyrinogen III methyltransferase		ECOLX		246
Uroporphyrinogen III synthase		Escherichia coli G3/10		246
Uroporphyrinogen-III synthase		Escherichia coli KTE108		246
Uroporphyrinogen-III synthase HemD family protein		Escherichia coli 1-110-08_S3_C1		246
Uroporphyrinogen-III synthase HemD family protein		Escherichia coli DEC6A		246
+1459

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X12614 Genomic DNA Translation: CAA31133.1 Y00883 Genomic DNA Translation: CAA68776.1 M17360 Genomic DNA Translation: AAA23956.1 M87049 Genomic DNA Translation: AAA67600.1 U00096 Genomic DNA Translation: AAC76807.1 AP009048 Genomic DNA Translation: BAE77497.1
PIRⁱ	E65184
NCBI Reference Sequences More...RefSeqⁱ	NP_418248.1, NC_000913.3 WP_000026046.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P09126
SMRⁱ	P09126
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262609, 4 interactors
IntActⁱ	P09126, 3 interactors
STRINGⁱ	316385.ECDH10B_3995

Proteomic databases

PaxDbⁱ	P09126
PRIDEⁱ	P09126

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	948587
Structural Biology Knowledgebase	Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76807; AAC76807; b3804 BAE77497; BAE77497; BAE77497
GeneIDⁱ	948587
KEGGⁱ	ecj:JW3776 eco:b3804
PATRICⁱ	fig\|1411691.4.peg.2904

Organism-specific databases

EchoBASEⁱ	EB0425
EcoGeneⁱ	EG10430 hemD

Phylogenomic databases

eggNOGⁱ	ENOG41076E6 Bacteria COG1587 LUCA
HOGENOMⁱ	HOG000247993
InParanoidⁱ	P09126
KEGG Orthology (KO) More...KOⁱ	K01719
OMAⁱ	TRPAEDC
PhylomeDBⁱ	P09126

Enzyme and pathway databases

UniPathwayⁱ	UPA00251; UER00320
BioCycⁱ	EcoCyc:UROGENIIISYN-MONOMER MetaCyc:UROGENIIISYN-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P09126

PROⁱ

PR:P09126

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06578 HemD, 1 hit
InterProⁱ	View protein in InterPro IPR036108 4pyrrol_syn_uPrphyn_synt_sf IPR003754 4pyrrol_synth_uPrphyn_synth
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02602 HEM4, 1 hit
SUPFAMⁱ	SSF69618 SSF69618, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HEM4_ECOLI
Accessionⁱ	P09126Primary (citable) accession number: P09126 Secondary accession number(s): Q2M8A9, Q47250
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 1, 1989
	Last modified:	March 28, 2018
	This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

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UniProtKB - P09126 (HEM4_ECOLI)

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Uroporphyrinogen-III synthase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protoporphyrin-IX biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: protoporphyrin-IX biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ