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Protein

Uroporphyrinogen-III synthase

Gene

hemD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • uroporphyrinogen-III synthase activity Source: EcoCyc

GO - Biological processi

  • protoporphyrinogen IX biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionLyase
Biological processPorphyrin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:UROGENIIISYN-MONOMER
MetaCyc:UROGENIIISYN-MONOMER
UniPathwayiUPA00251; UER00320

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:hemD
Ordered Locus Names:b3804, JW3776
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10430 hemD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352431 – 246Uroporphyrinogen-III synthaseAdd BLAST246

Proteomic databases

PaxDbiP09126
PRIDEiP09126

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262609, 4 interactors
IntActiP09126, 3 interactors
STRINGi316385.ECDH10B_3995

Structurei

3D structure databases

ProteinModelPortaliP09126
SMRiP09126
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG41076E6 Bacteria
COG1587 LUCA
HOGENOMiHOG000247993
InParanoidiP09126
KOiK01719
OMAiTRPAEDC
PhylomeDBiP09126

Family and domain databases

CDDicd06578 HemD, 1 hit
InterProiView protein in InterPro
IPR036108 4pyrrol_syn_uPrphyn_synt_sf
IPR003754 4pyrrol_synth_uPrphyn_synth
PfamiView protein in Pfam
PF02602 HEM4, 1 hit
SUPFAMiSSF69618 SSF69618, 1 hit

Sequencei

Sequence statusi: Complete.

P09126-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL
60 70 80 90 100
GESDLLFALS QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG
110 120 130 140 150
QKILYPQDRE ISEVLLQLPE LQNIAGKRAL ILRGNGGREL IGDTLTARGA
160 170 180 190 200
EVTFCECYQR CAIHYDGAEE AMRWQAREVT MVVVTSGEML QQLWSLIPQW
210 220 230 240
YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ
Length:246
Mass (Da):27,798
Last modified:July 1, 1989 - v1
Checksum:i0BF7453C72395A0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146T → A in AAA23956 (PubMed:3040684).Curated1
Sequence conflicti236A → R (PubMed:3320969).Curated1
Sequence conflicti236A → R (PubMed:3277628).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12614 Genomic DNA Translation: CAA31133.1
Y00883 Genomic DNA Translation: CAA68776.1
M17360 Genomic DNA Translation: AAA23956.1
M87049 Genomic DNA Translation: AAA67600.1
U00096 Genomic DNA Translation: AAC76807.1
AP009048 Genomic DNA Translation: BAE77497.1
PIRiE65184
RefSeqiNP_418248.1, NC_000913.3
WP_000026046.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76807; AAC76807; b3804
BAE77497; BAE77497; BAE77497
GeneIDi948587
KEGGiecj:JW3776
eco:b3804
PATRICifig|1411691.4.peg.2904

Similar proteinsi

Entry informationi

Entry nameiHEM4_ECOLI
AccessioniPrimary (citable) accession number: P09126
Secondary accession number(s): Q2M8A9, Q47250
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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