UniProtKB - P09110 (THIK_HUMAN)
Protein
3-ketoacyl-CoA thiolase, peroxisomal
Gene
ACAA1
Organism
Homo sapiens (Human)
Status
Functioni
Responsible for the thiolytic cleavage of straight chain 3-oxoacyl-CoAs. Catalyzes the cleavage of short, medium and long straight chain 3-oxoacyl-CoAs, medium chain 3-oxoacyl-CoAs being the best substrates.By similarity
Catalytic activityi
- EC:2.3.1.16By similarityThis reaction proceeds in the backwardBy similarity direction.
- EC:2.3.1.9By similarityThis reaction proceeds in the backwardBy similarity direction.
- EC:2.3.1.155By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 123 | Acyl-thioester intermediateBy similarity | 1 | |
Active sitei | 377 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 408 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- acetate CoA-transferase activity Source: Reactome
- acetyl-CoA C-acyltransferase activity Source: UniProtKB
- acetyl-CoA C-myristoyltransferase activity Source: UniProtKB
- palmitoyl-CoA oxidase activity Source: UniProtKB
GO - Biological processi
- alpha-linolenic acid metabolic process Source: Reactome
- bile acid metabolic process Source: UniProtKB
- fatty acid beta-oxidation Source: UniProtKB
- fatty acid beta-oxidation using acyl-CoA oxidase Source: Reactome
- neutrophil degranulation Source: Reactome
- phenylacetate catabolic process Source: GO_Central
- protein targeting to peroxisome Source: Reactome
- very long-chain fatty acid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00752-MONOMER |
PathwayCommonsi | P09110 |
Reactomei | R-HSA-2046106, alpha-linolenic acid (ALA) metabolism R-HSA-390247, Beta-oxidation of very long chain fatty acids R-HSA-6798695, Neutrophil degranulation R-HSA-9033241, Peroxisomal protein import R-HSA-9033500, TYSND1 cleaves peroxisomal proteins |
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:82, ACAA1 |
MIMi | 604054, gene |
neXtProti | NX_P09110 |
VEuPathDBi | HostDB:ENSG00000060971.17 |
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular region Source: Reactome
Peroxisome
- peroxisomal matrix Source: Reactome
- peroxisome Source: UniProtKB
Other locations
- intracellular membrane-bounded organelle Source: HPA
- membrane Source: UniProtKB
- specific granule lumen Source: Reactome
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Organism-specific databases
DisGeNETi | 30 |
OpenTargetsi | ENSG00000060971 |
PharmGKBi | PA24419 |
Miscellaneous databases
Pharosi | P09110, Tbio |
Chemistry databases
DrugBanki | DB09069, Trimetazidine |
Genetic variation databases
BioMutai | ACAA1 |
DMDMi | 135751 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 26 | PeroxisomeCombined sourcesAdd BLAST | 26 | |
ChainiPRO_0000034067 | 27 – 424 | 3-ketoacyl-CoA thiolase, peroxisomalAdd BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 59 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 60 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P09110 |
jPOSTi | P09110 |
MassIVEi | P09110 |
MaxQBi | P09110 |
PaxDbi | P09110 |
PeptideAtlasi | P09110 |
PRIDEi | P09110 |
ProteomicsDBi | 33816 52200 [P09110-1] |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00012828 |
UCD-2DPAGEi | P09110 |
PTM databases
CarbonylDBi | P09110 |
iPTMneti | P09110 |
MetOSitei | P09110 |
PhosphoSitePlusi | P09110 |
SwissPalmi | P09110 |
Expressioni
Inductioni
Peroxisomal thiolase is markedly induced (at the level of transcription) by various hypolipidemic compounds in parallel with the other two enzymes of the peroxisomal beta-oxidation system.By similarity
Gene expression databases
Bgeei | ENSG00000060971, Expressed in nephron tubule and 248 other tissues |
ExpressionAtlasi | P09110, baseline and differential |
Genevisiblei | P09110, HS |
Organism-specific databases
HPAi | ENSG00000060971, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 106548, 50 interactors |
IntActi | P09110, 14 interactors |
STRINGi | 9606.ENSP00000333664 |
Miscellaneous databases
RNActi | P09110, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09110 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09110 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1389, Eukaryota |
GeneTreei | ENSGT01010000222488 |
InParanoidi | P09110 |
OMAi | YHETRAD |
PhylomeDBi | P09110 |
TreeFami | TF332308 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P09110-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQRLQVVLGH LRGPADSGWM PQAAPCLSGA PQASAADVVV VHGRRTAICR
60 70 80 90 100
AGRGGFKDTT PDELLSAVMT AVLKDVNLRP EQLGDICVGN VLQPGAGAIM
110 120 130 140 150
ARIAQFLSDI PETVPLSTVN RQCSSGLQAV ASIAGGIRNG SYDIGMACGV
160 170 180 190 200
ESMSLADRGN PGNITSRLME KEKARDCLIP MGITSENVAE RFGISREKQD
210 220 230 240 250
TFALASQQKA ARAQSKGCFQ AEIVPVTTTV HDDKGTKRSI TVTQDEGIRP
260 270 280 290 300
STTMEGLAKL KPAFKKDGST TAGNSSQVSD GAAAILLARR SKAEELGLPI
310 320 330 340 350
LGVLRSYAVV GVPPDIMGIG PAYAIPVALQ KAGLTVSDVD IFEINEAFAS
360 370 380 390 400
QAAYCVEKLR LPPEKVNPLG GAVALGHPLG CTGARQVITL LNELKRRGKR
410 420
AYGVVSMCIG TGMGAAAVFE YPGN
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JDE9 | C9JDE9_HUMAN | 3-ketoacyl-CoA thiolase, peroxisoma... | ACAA1 | 383 | Annotation score: | ||
H7C131 | H7C131_HUMAN | 3-ketoacyl-CoA thiolase, peroxisoma... | ACAA1 | 290 | Annotation score: | ||
H0Y4D4 | H0Y4D4_HUMAN | 3-ketoacyl-CoA thiolase, peroxisoma... | ACAA1 | 237 | Annotation score: | ||
B4DVF4 | B4DVF4_HUMAN | 3-ketoacyl-CoA thiolase, peroxisoma... | ACAA1 | 153 | Annotation score: | ||
F2Z2Q6 | F2Z2Q6_HUMAN | 3-ketoacyl-CoA thiolase, peroxisoma... | ACAA1 | 113 | Annotation score: | ||
F2Z3P7 | F2Z3P7_HUMAN | 3-ketoacyl-CoA thiolase, peroxisoma... | ACAA1 | 121 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_011904 | 172 | E → D. Corresponds to variant dbSNP:rs156265Ensembl. | 1 | |
Natural variantiVAR_069148 | 387 | V → A1 PublicationCorresponds to variant dbSNP:rs2229528Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046195 | 149 – 181 | Missing in isoform 2. 1 PublicationAdd BLAST | 33 | |
Alternative sequenceiVSP_046196 | 272 – 331 | Missing in isoform 2. 1 PublicationAdd BLAST | 60 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12966 mRNA Translation: CAA31412.1 X14813 mRNA Translation: CAA32918.1 X65140 Genomic DNA Translation: CAA46270.1 X65148 Genomic DNA Translation: CAA46271.1 AP006309 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64516.1 BC000635 mRNA Translation: AAH00635.1 BC011977 mRNA Translation: AAH11977.1 BC014474 mRNA Translation: AAH14474.1 |
CCDSi | CCDS2673.1 [P09110-1] CCDS46794.1 [P09110-2] |
PIRi | S17515, XUHUAB |
RefSeqi | NP_001123882.1, NM_001130410.1 [P09110-2] NP_001598.1, NM_001607.3 [P09110-1] |
Genome annotation databases
Ensembli | ENST00000301810; ENSP00000301810; ENSG00000060971 [P09110-2] ENST00000333167; ENSP00000333664; ENSG00000060971 [P09110-1] |
GeneIDi | 30 |
KEGGi | hsa:30 |
UCSCi | uc003cht.4, human [P09110-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12966 mRNA Translation: CAA31412.1 X14813 mRNA Translation: CAA32918.1 X65140 Genomic DNA Translation: CAA46270.1 X65148 Genomic DNA Translation: CAA46271.1 AP006309 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64516.1 BC000635 mRNA Translation: AAH00635.1 BC011977 mRNA Translation: AAH11977.1 BC014474 mRNA Translation: AAH14474.1 |
CCDSi | CCDS2673.1 [P09110-1] CCDS46794.1 [P09110-2] |
PIRi | S17515, XUHUAB |
RefSeqi | NP_001123882.1, NM_001130410.1 [P09110-2] NP_001598.1, NM_001607.3 [P09110-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IIK | X-ray | 2.55 | A/B | 30-423 | [»] | |
SMRi | P09110 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106548, 50 interactors |
IntActi | P09110, 14 interactors |
STRINGi | 9606.ENSP00000333664 |
Chemistry databases
DrugBanki | DB09069, Trimetazidine |
PTM databases
CarbonylDBi | P09110 |
iPTMneti | P09110 |
MetOSitei | P09110 |
PhosphoSitePlusi | P09110 |
SwissPalmi | P09110 |
Genetic variation databases
BioMutai | ACAA1 |
DMDMi | 135751 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00012828 |
UCD-2DPAGEi | P09110 |
Proteomic databases
EPDi | P09110 |
jPOSTi | P09110 |
MassIVEi | P09110 |
MaxQBi | P09110 |
PaxDbi | P09110 |
PeptideAtlasi | P09110 |
PRIDEi | P09110 |
ProteomicsDBi | 33816 52200 [P09110-1] |
Protocols and materials databases
Antibodypediai | 1631, 309 antibodies |
DNASUi | 30 |
Genome annotation databases
Ensembli | ENST00000301810; ENSP00000301810; ENSG00000060971 [P09110-2] ENST00000333167; ENSP00000333664; ENSG00000060971 [P09110-1] |
GeneIDi | 30 |
KEGGi | hsa:30 |
UCSCi | uc003cht.4, human [P09110-1] |
Organism-specific databases
CTDi | 30 |
DisGeNETi | 30 |
GeneCardsi | ACAA1 |
HGNCi | HGNC:82, ACAA1 |
HPAi | ENSG00000060971, Tissue enhanced (liver) |
MIMi | 604054, gene |
neXtProti | NX_P09110 |
OpenTargetsi | ENSG00000060971 |
PharmGKBi | PA24419 |
VEuPathDBi | HostDB:ENSG00000060971.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1389, Eukaryota |
GeneTreei | ENSGT01010000222488 |
InParanoidi | P09110 |
OMAi | YHETRAD |
PhylomeDBi | P09110 |
TreeFami | TF332308 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BioCyci | MetaCyc:HS00752-MONOMER |
PathwayCommonsi | P09110 |
Reactomei | R-HSA-2046106, alpha-linolenic acid (ALA) metabolism R-HSA-390247, Beta-oxidation of very long chain fatty acids R-HSA-6798695, Neutrophil degranulation R-HSA-9033241, Peroxisomal protein import R-HSA-9033500, TYSND1 cleaves peroxisomal proteins |
Miscellaneous databases
BioGRID-ORCSi | 30, 8 hits in 879 CRISPR screens |
ChiTaRSi | ACAA1, human |
EvolutionaryTracei | P09110 |
GeneWikii | ACAA1 |
GenomeRNAii | 30 |
Pharosi | P09110, Tbio |
PROi | PR:P09110 |
RNActi | P09110, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000060971, Expressed in nephron tubule and 248 other tissues |
ExpressionAtlasi | P09110, baseline and differential |
Genevisiblei | P09110, HS |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THIK_HUMAN | |
Accessioni | P09110Primary (citable) accession number: P09110 Secondary accession number(s): G5E935, Q96CA6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 217 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM