Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein disulfide-isomerase

Gene

P4hb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP (By similarity). Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).By similarity1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei55NucleophileBy similarity1
Sitei56Contributes to redox potential valueBy similarity1
Sitei57Contributes to redox potential valueBy similarity1
Active sitei58NucleophileBy similarity1
Sitei122Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei399NucleophileBy similarity1
Sitei400Contributes to redox potential valueBy similarity1
Sitei401Contributes to redox potential valueBy similarity1
Active sitei402NucleophileBy similarity1
Sitei463Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone, Isomerase

Enzyme and pathway databases

ReactomeiR-MMU-1650814 Collagen biosynthesis and modifying enzymes
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-8866423 VLDL assembly
R-MMU-8957275 Post-translational protein phosphorylation
R-MMU-8963888 Chylomicron assembly
R-MMU-9020591 Interleukin-12 signaling
R-MMU-9020933 Interleukin-23 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Alternative name(s):
Cellular thyroid hormone-binding protein
Endoplasmic reticulum resident protein 59
Short name:
ER protein 59
Short name:
ERp59
Prolyl 4-hydroxylase subunit beta
p55
Gene namesi
Name:P4hb
Synonyms:Pdia1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97464 P4hb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000003419620 – 509Protein disulfide-isomeraseAdd BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 58Redox-activePROSITE-ProRule annotation
Modified residuei202N6-acetyllysineCombined sources1
Modified residuei224N6-succinyllysineCombined sources1
Modified residuei273N6-succinyllysineCombined sources1
Modified residuei359PhosphoserineBy similarity1
Disulfide bondi399 ↔ 402Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP09103
MaxQBiP09103
PaxDbiP09103
PeptideAtlasiP09103
PRIDEiP09103

2D gel databases

COMPLUYEAST-2DPAGEiP09103
REPRODUCTION-2DPAGEiP09103
SWISS-2DPAGEiP09103

PTM databases

iPTMnetiP09103
PhosphoSitePlusiP09103
SwissPalmiP09103

Expressioni

Gene expression databases

BgeeiENSMUSG00000025130 Expressed in 290 organ(s), highest expression level in lacrimal gland
CleanExiMM_P4HB
ExpressionAtlasiP09103 baseline and differential
GenevisibleiP09103 MM

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with the protein microsomal triglyceride transfer MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Interacts with ERO1B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202008, 2 interactors
CORUMiP09103
ELMiP09103
IntActiP09103, 12 interactors
MINTiP09103
STRINGi10090.ENSMUSP00000026122

Structurei

3D structure databases

ProteinModelPortaliP09103
SMRiP09103
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 136Thioredoxin 1PROSITE-ProRule annotationAdd BLAST117
Domaini348 – 477Thioredoxin 2PROSITE-ProRule annotationAdd BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi506 – 509Prevents secretion from ER4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
GeneTreeiENSGT00930000150840
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP09103
KOiK09580
OMAiHTRIFEF
OrthoDBiEOG091G08WM
TreeFamiTF106381

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P09103-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSRALLCLA LAWAARVGAD ALEEEDNVLV LKKSNFEEAL AAHKYLLVEF
60 70 80 90 100
YAPWCGHCKA LAPEYAKAAA KLKAEGSEIR LAKVDATEES DLAQQYGVRG
110 120 130 140 150
YPTIKFFKNG DTASPKEYTA GREADDIVNW LKKRTGPAAT TLSDTAAAES
160 170 180 190 200
LVDSSEVTVI GFFKDVESDS AKQFLLAAEA IDDIPFGITS NSGVFSKYQL
210 220 230 240 250
DKDGVVLFKK FDEGRNNFEG EITKEKLLDF IKHNQLPLVI EFTEQTAPKI
260 270 280 290 300
FGGEIKTHIL LFLPKSVSDY DGKLSSFKRA AEGFKGKILF IFIDSDHTDN
310 320 330 340 350
QRILEFFGLK KEECPAVRLI TLEEEMTKYK PESDELTAEK ITEFCHRFLE
360 370 380 390 400
GKIKPHLMSQ EVPEDWDKQP VKVLVGANFE EVAFDEKKNV FVEFYAPWCG
410 420 430 440 450
HCKQLAPIWD KLGETYKDHE NIIIAKMDST ANEVEAVKVH SFPTLKFFPA
460 470 480 490 500
SADRTVIDYN GERTLDGFKK FLESGGQDGA GDDEDLDLEE ALEPDMEEDD

DQKAVKDEL
Length:509
Mass (Da):57,058
Last modified:July 27, 2011 - v2
Checksum:i1FB0AA8ACCDB13EA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q8G8E9Q8G8_MOUSE
Protein disulfide-isomerase
P4hb
165Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68A → R in CAA29759 (PubMed:2830592).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06453 mRNA Translation: CAA29759.1
J05185 mRNA Translation: AAA39906.1
AK150002 mRNA Translation: BAE29230.1
AK150422 mRNA Translation: BAE29545.1
AK150805 mRNA Translation: BAE29868.1
AK152141 mRNA Translation: BAE30979.1
AK152217 mRNA Translation: BAE31045.1
AK159606 mRNA Translation: BAE35225.1
AK159965 mRNA Translation: BAE35520.1
AK168330 mRNA Translation: BAE40268.1
AK168893 mRNA Translation: BAE40710.1
AK170603 mRNA Translation: BAE41907.1
AL663030 Genomic DNA No translation available.
BC008549 mRNA Translation: AAH08549.1
BC093512 mRNA Translation: AAH93512.1
CCDSiCCDS25742.1
PIRiA34930 ISMSSS
RefSeqiNP_035162.1, NM_011032.2
UniGeneiMm.16660

Genome annotation databases

EnsembliENSMUST00000026122; ENSMUSP00000026122; ENSMUSG00000025130
GeneIDi18453
KEGGimmu:18453
UCSCiuc007mti.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06453 mRNA Translation: CAA29759.1
J05185 mRNA Translation: AAA39906.1
AK150002 mRNA Translation: BAE29230.1
AK150422 mRNA Translation: BAE29545.1
AK150805 mRNA Translation: BAE29868.1
AK152141 mRNA Translation: BAE30979.1
AK152217 mRNA Translation: BAE31045.1
AK159606 mRNA Translation: BAE35225.1
AK159965 mRNA Translation: BAE35520.1
AK168330 mRNA Translation: BAE40268.1
AK168893 mRNA Translation: BAE40710.1
AK170603 mRNA Translation: BAE41907.1
AL663030 Genomic DNA No translation available.
BC008549 mRNA Translation: AAH08549.1
BC093512 mRNA Translation: AAH93512.1
CCDSiCCDS25742.1
PIRiA34930 ISMSSS
RefSeqiNP_035162.1, NM_011032.2
UniGeneiMm.16660

3D structure databases

ProteinModelPortaliP09103
SMRiP09103
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202008, 2 interactors
CORUMiP09103
ELMiP09103
IntActiP09103, 12 interactors
MINTiP09103
STRINGi10090.ENSMUSP00000026122

PTM databases

iPTMnetiP09103
PhosphoSitePlusiP09103
SwissPalmiP09103

2D gel databases

COMPLUYEAST-2DPAGEiP09103
REPRODUCTION-2DPAGEiP09103
SWISS-2DPAGEiP09103

Proteomic databases

EPDiP09103
MaxQBiP09103
PaxDbiP09103
PeptideAtlasiP09103
PRIDEiP09103

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026122; ENSMUSP00000026122; ENSMUSG00000025130
GeneIDi18453
KEGGimmu:18453
UCSCiuc007mti.1 mouse

Organism-specific databases

CTDi5034
MGIiMGI:97464 P4hb

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
GeneTreeiENSGT00930000150840
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP09103
KOiK09580
OMAiHTRIFEF
OrthoDBiEOG091G08WM
TreeFamiTF106381

Enzyme and pathway databases

ReactomeiR-MMU-1650814 Collagen biosynthesis and modifying enzymes
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-8866423 VLDL assembly
R-MMU-8957275 Post-translational protein phosphorylation
R-MMU-8963888 Chylomicron assembly
R-MMU-9020591 Interleukin-12 signaling
R-MMU-9020933 Interleukin-23 signaling

Miscellaneous databases

ChiTaRSiP4hb mouse
PROiPR:P09103
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025130 Expressed in 290 organ(s), highest expression level in lacrimal gland
CleanExiMM_P4HB
ExpressionAtlasiP09103 baseline and differential
GenevisibleiP09103 MM

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA1_MOUSE
AccessioniPrimary (citable) accession number: P09103
Secondary accession number(s): Q922C8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again