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Protein

Serine protease inhibitor Kazal-type 1

Gene

Spink1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:3428272, PubMed:14645103, PubMed:22228629). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (PubMed:16083722).4 Publications
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production (PubMed:9828198, PubMed:14645103, PubMed:16083722, PubMed:22228629).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei42 – 43Reactive bond for trypsin2 Publications2
Sitei44 – 45Necessary for sperm binding1 Publication2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.011

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 1Imported
Alternative name(s):
P121 Publication
Prostatic secretory glycoprotein1 Publication
Serine protease inhibitor Kazal-type 31 Publication
Gene namesi
Name:Spink1Imported
Synonyms:Spink31 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:106202 Spink1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Lethal with no survival past two weeks of age. Animals are small and show severe, progressive degeneration of pancreatic tissue associated with autophagic cell death.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42R → L: Abolishes trypsin inhibitor activity. No effect on sperm binding. 1 Publication1
Mutagenesisi44Y → V: Severely impairs sperm binding. No effect on trypsin inhibitor activity. 1 Publication1
Mutagenesisi45D → G: Fails to bind sperm. No effect on trypsin inhibitor activity. 1 Publication1
Mutagenesisi66R → G: No effect on trypsin inhibitor activity or sperm binding. 1 Publication1
Mutagenesisi67K → S: No effect on trypsin inhibitor activity or sperm binding. 1 Publication1
Mutagenesisi68R → T: No effect on trypsin inhibitor activity or sperm binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001656824 – 80Serine protease inhibitor Kazal-type 1Add BLAST57

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 62PROSITE-ProRule annotation
Disulfide bondi40 ↔ 59PROSITE-ProRule annotation
Disulfide bondi48 ↔ 80PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP09036
PeptideAtlasiP09036
PRIDEiP09036

Expressioni

Tissue specificityi

In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate.1 Publication

Developmental stagei

In the seminal vesicle, not expressed during prepubertal stages; expression coincides with maturation.1 Publication

Inductioni

By androgens in adult male sex accessory glands. Expressed constitutively in pancreas.1 Publication

Gene expression databases

BgeeiENSMUSG00000024503 Expressed in 99 organ(s), highest expression level in prostate gland ventral lobe
CleanExiMM_SPINK3
GenevisibleiP09036 MM

Interactioni

Protein-protein interaction databases

IntActiP09036, 1 interactor
MINTiP09036
STRINGi10090.ENSMUSP00000025381

Structurei

3D structure databases

ProteinModelPortaliP09036
SMRiP09036
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 80Kazal-likePROSITE-ProRule annotationAdd BLAST54

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3649 Eukaryota
ENOG410YC3T LUCA
GeneTreeiENSGT00530000064228
HOGENOMiHOG000090244
HOVERGENiHBG006182
InParanoidiP09036
OMAiVPVLIQK
OrthoDBiEOG091G14HG
PhylomeDBiP09036

Family and domain databases

InterProiView protein in InterPro
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR001239 Prot_inh_Kazal-m
PfamiView protein in Pfam
PF00050 Kazal_1, 1 hit
PRINTSiPR00290 KAZALINHBTR
SMARTiView protein in SMART
SM00280 KAZAL, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
PROSITEiView protein in PROSITE
PS00282 KAZAL_1, 1 hit
PS51465 KAZAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09036-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVAVIFLLS ALALLSLAGN TFSAKVTGKE ASCHDAVAGC PRIYDPVCGT
60 70 80
DGITYANECV LCFENRKRIE PVLIRKGGPC
Length:80
Mass (Da):8,488
Last modified:November 1, 1988 - v1
Checksum:i4DC1F2EC4804CCA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06342 mRNA Translation: CAA29648.1
AK007841 mRNA Translation: BAB25298.1
AK007985 mRNA Translation: BAB25389.1
BC086887 mRNA Translation: AAH86887.1
CCDSiCCDS37803.1
PIRiS01498
RefSeqiNP_033284.1, NM_009258.5
UniGeneiMm.272

Genome annotation databases

EnsembliENSMUST00000025381; ENSMUSP00000025381; ENSMUSG00000024503
GeneIDi20730
KEGGimmu:20730
UCSCiuc008eul.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06342 mRNA Translation: CAA29648.1
AK007841 mRNA Translation: BAB25298.1
AK007985 mRNA Translation: BAB25389.1
BC086887 mRNA Translation: AAH86887.1
CCDSiCCDS37803.1
PIRiS01498
RefSeqiNP_033284.1, NM_009258.5
UniGeneiMm.272

3D structure databases

ProteinModelPortaliP09036
SMRiP09036
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09036, 1 interactor
MINTiP09036
STRINGi10090.ENSMUSP00000025381

Protein family/group databases

MEROPSiI01.011

Proteomic databases

PaxDbiP09036
PeptideAtlasiP09036
PRIDEiP09036

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025381; ENSMUSP00000025381; ENSMUSG00000024503
GeneIDi20730
KEGGimmu:20730
UCSCiuc008eul.2 mouse

Organism-specific databases

CTDi6690
MGIiMGI:106202 Spink1

Phylogenomic databases

eggNOGiKOG3649 Eukaryota
ENOG410YC3T LUCA
GeneTreeiENSGT00530000064228
HOGENOMiHOG000090244
HOVERGENiHBG006182
InParanoidiP09036
OMAiVPVLIQK
OrthoDBiEOG091G14HG
PhylomeDBiP09036

Miscellaneous databases

ChiTaRSiSpink1 mouse
PROiPR:P09036
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024503 Expressed in 99 organ(s), highest expression level in prostate gland ventral lobe
CleanExiMM_SPINK3
GenevisibleiP09036 MM

Family and domain databases

InterProiView protein in InterPro
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR001239 Prot_inh_Kazal-m
PfamiView protein in Pfam
PF00050 Kazal_1, 1 hit
PRINTSiPR00290 KAZALINHBTR
SMARTiView protein in SMART
SM00280 KAZAL, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
PROSITEiView protein in PROSITE
PS00282 KAZAL_1, 1 hit
PS51465 KAZAL_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISK1_MOUSE
AccessioniPrimary (citable) accession number: P09036
Secondary accession number(s): Q5M9M3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 7, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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