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UniProtKB - P09029 (PURK_ECOLI)
Protein
N5-carboxyaminoimidazole ribonucleotide synthase
Gene
purK
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).
UniRule annotation1 PublicationCaution
Was originally thought to be the ATPase subunit of phosphoribosylaminoimidazole carboxylase, with catalytic subunit PurE.1 Publication
Catalytic activityi
- 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H+ + phosphateUniRule annotation1 PublicationEC:6.3.4.18UniRule annotation1 Publication
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotation1 Publication This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 80 | ATPUniRule annotation2 Publications | 1 | |
Binding sitei | 120 | ATPUniRule annotation2 Publications | 1 | |
Binding sitei | 161 | ATPUniRule annotation2 Publications | 1 | |
Binding sitei | 184 | ATPUniRule annotation2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 125 – 131 | ATPUniRule annotation2 Publications | 7 | |
Nucleotide bindingi | 153 – 156 | ATPUniRule annotation2 Publications | 4 | |
Nucleotide bindingi | 237 – 238 | ATPUniRule annotation2 Publications | 2 |
GO - Molecular functioni
- 5-(carboxyamino)imidazole ribonucleotide synthase activity Source: EcoCyc
- ATP binding Source: EcoCyc
- metal ion binding Source: InterPro
- phosphoribosylaminoimidazole carboxylase activity Source: EcoliWiki
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PURK-MONOMER |
BRENDAi | 6.3.4.18, 2026 |
SABIO-RKi | P09029 |
UniPathwayi | UPA00074;UER00942 |
Names & Taxonomyi
Protein namesi | Recommended name: N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotationCurated (EC:6.3.4.18UniRule annotation1 Publication)Short name: N5-CAIR synthaseUniRule annotationCurated Alternative name(s): 5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotationCurated |
Gene namesi | Name:purKUniRule annotation Ordered Locus Names:b0522Imported, JW0511Imported |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074997 | 1 – 355 | N5-carboxyaminoimidazole ribonucleotide synthaseAdd BLAST | 355 |
Proteomic databases
jPOSTi | P09029 |
PaxDbi | P09029 |
PRIDEi | P09029 |
2D gel databases
SWISS-2DPAGEi | P09029 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsProtein-protein interaction databases
BioGRIDi | 4259875, 17 interactors |
IntActi | P09029, 5 interactors |
STRINGi | 511145.b0522 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09029 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09029 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 84 – 267 | ATP-graspUniRule annotationAdd BLAST | 184 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0026, Bacteria |
HOGENOMi | CLU_011534_0_0_6 |
InParanoidi | P09029 |
PhylomeDBi | P09029 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_01928, PurK, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR003135, ATP-grasp_carboxylate-amine IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR005875, PurK IPR040686, PurK_C IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF02222, ATP-grasp, 1 hit PF17769, PurK_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01161, purK, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
i Sequence
Sequence statusi: Complete.
P09029-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKQVCVLGNG QLGRMLRQAG EPLGIAVWPV GLDAEPAAVP FQQSVITAEI
60 70 80 90 100
ERWPETALTR ELARHPAFVN RDVFPIIADR LTQKQLFDKL HLPTAPWQLL
110 120 130 140 150
AERSEWPAVF DRLGELAIVK RRTGGYDGRG QWRLRANETE QLPAECYGEC
160 170 180 190 200
IVEQGINFSG EVSLVGARGF DGSTVFYPLT HNLHQDGILR TSVAFPQANA
210 220 230 240 250
QQQAQAEEML SAIMQELGYV GVMAMECFVT PQGLLINELA PRVHNSGHWT
260 270 280 290 300
QNGASISQFE LHLRAITDLP LPQPVVNNPS VMINLIGSDV NYDWLKLPLV
310 320 330 340 350
HLHWYDKEVR PGRKVGHLNL TDSDTSRLTA TLEALIPLLP PEYASGVIWA
QSKFG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 64 – 65 | RH → PD in CAA31421 (PubMed:2464576).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12982 Genomic DNA Translation: CAA31421.1 M19657 Genomic DNA Translation: AAA24450.1 U82664 Genomic DNA Translation: AAB40275.1 U00096 Genomic DNA Translation: AAC73624.1 AP009048 Genomic DNA Translation: BAE76299.1 |
PIRi | JU0001, DCECPK |
RefSeqi | NP_415055.1, NC_000913.3 WP_000815571.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73624; AAC73624; b0522 BAE76299; BAE76299; BAE76299 |
GeneIDi | 945153 |
KEGGi | ecj:JW0511 eco:b0522 |
PATRICi | fig|1411691.4.peg.1756 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12982 Genomic DNA Translation: CAA31421.1 M19657 Genomic DNA Translation: AAA24450.1 U82664 Genomic DNA Translation: AAB40275.1 U00096 Genomic DNA Translation: AAC73624.1 AP009048 Genomic DNA Translation: BAE76299.1 |
PIRi | JU0001, DCECPK |
RefSeqi | NP_415055.1, NC_000913.3 WP_000815571.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B6R | X-ray | 2.10 | A | 1-355 | [»] | |
1B6S | X-ray | 2.50 | A/B/C/D | 1-355 | [»] | |
3ETH | X-ray | 1.60 | A/B | 1-355 | [»] | |
3ETJ | X-ray | 1.60 | A/B | 1-355 | [»] | |
SMRi | P09029 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259875, 17 interactors |
IntActi | P09029, 5 interactors |
STRINGi | 511145.b0522 |
2D gel databases
SWISS-2DPAGEi | P09029 |
Proteomic databases
jPOSTi | P09029 |
PaxDbi | P09029 |
PRIDEi | P09029 |
Genome annotation databases
EnsemblBacteriai | AAC73624; AAC73624; b0522 BAE76299; BAE76299; BAE76299 |
GeneIDi | 945153 |
KEGGi | ecj:JW0511 eco:b0522 |
PATRICi | fig|1411691.4.peg.1756 |
Organism-specific databases
EchoBASEi | EB0789 |
Phylogenomic databases
eggNOGi | COG0026, Bacteria |
HOGENOMi | CLU_011534_0_0_6 |
InParanoidi | P09029 |
PhylomeDBi | P09029 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00942 |
BioCyci | EcoCyc:PURK-MONOMER |
BRENDAi | 6.3.4.18, 2026 |
SABIO-RKi | P09029 |
Miscellaneous databases
EvolutionaryTracei | P09029 |
PROi | PR:P09029 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_01928, PurK, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR003135, ATP-grasp_carboxylate-amine IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR005875, PurK IPR040686, PurK_C IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF02222, ATP-grasp, 1 hit PF17769, PurK_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01161, purK, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PURK_ECOLI | |
Accessioni | P09029Primary (citable) accession number: P09029 Secondary accession number(s): Q2MBQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families