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Protein

Homeobox protein Hox-A1

Gene

Hoxa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sequence-specific transcription factor (PubMed:29465778). Regulates multiple developmental processes including brainstem, inner and outer ear, abducens nerve and cardiovascular development and morphogenesis as well as cognition and behavior (By similarity). Also part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments (By similarity). Activates transcription in the presence of PBX1A and PKNOX1 (PubMed:29465778).By similarity1 Publication
The homeobox domain presumably directs sequence-specific DNA binding. The N-terminal portion of ERA-1-993 may be involved in interactions with one or more other regulatory proteins. Such an interaction could regulate either the DNA-binding activity or the transcriptional regulatory activity of ERA-1-993.
The homeoboxless ERA-1-399 protein could act as a competitive inhibitor of the ERA-1-993 protein by competing for interaction with regulatory protein(s) while being unable to bind to DNA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi225 – 284HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Hox-A1
Alternative name(s):
Early retinoic acid 1
Homeobox protein Hox-1.6
Homeoboxless protein ERA-1-399
Homeotic protein ERA-1-993
Gene namesi
Name:Hoxa1
Synonyms:Era-1, Hox-1.6, Hoxa-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96170 Hoxa1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 224Missing : Loss of interaction with OGT. 1 PublicationAdd BLAST224
Mutagenesisi1 – 40Missing : Interacts with OGT. 1 PublicationAdd BLAST40
Mutagenesisi60 – 142Missing : Loss of interaction with OGT. 1 PublicationAdd BLAST83
Mutagenesisi60 – 70Missing : Loss of interaction with OGT. 1 PublicationAdd BLAST11
Mutagenesisi71 – 199Missing : Loss of interaction with OGT. 1 PublicationAdd BLAST129
Mutagenesisi203 – 204WM → AA: Interacts with OGT. 1 Publication2
Mutagenesisi225 – 284Missing : Interacts with OGT. 1 PublicationAdd BLAST60
Mutagenesisi272 – 275WFQN → SVAA: Interacts with OGT. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002000311 – 331Homeobox protein Hox-A1Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi149O-linked (GlcNAc) threonine1 Publication1

Post-translational modificationi

Glycosylated by OGT.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP09022
PRIDEiP09022

Expressioni

Developmental stagei

Expressed along the entire length of the primitive streak. In early neurogenesis it is expressed in lateral and paraxial mesoderm, endoderm and superficial ectoderm or in the neural tube. From late neurogenesis to mid-embryogenesis, it presents similar spatial domains in the lateral mesoderm, endoderm and superficial ectoderm but is not detectable in any part of the hindbrain. At day 8-8.5 post-coitum found in the embryonic mesoderm, anterior to the first somite, up to the cephalic region at the level of the foregut and developing heart. At day 9 post-coitum found in an anterior part of the developing foregut and is restricted to an epithelial cell type. Expression in the gut is found at the level of the second to third branchial bars.1 Publication

Inductioni

By retinoic acid.

Gene expression databases

CleanExiMM_HOXA1

Interactioni

Subunit structurei

Interacts with OGT (via TPR repeats domain); the interaction takes place mainly in the nucleus.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-59869N
IntActiP09022, 62 interactors
STRINGi10090.ENSMUSP00000000964

Structurei

3D structure databases

ProteinModelPortaliP09022
SMRiP09022
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 199Interaction with OGT1 PublicationAdd BLAST129

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi200 – 205Antp-type hexapeptide6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi60 – 70Poly-HisAdd BLAST11
Compositional biasi138 – 142Poly-His5

Sequence similaritiesi

Belongs to the Antp homeobox family. Labial subfamily.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0489 Eukaryota
ENOG410ZTBY LUCA
HOGENOMiHOG000171545
HOVERGENiHBG006089
InParanoidiP09022

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR020479 Homeobox_metazoa
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PRINTSiPR00024 HOMEOBOX
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform ERA-1-993 (identifier: P09022-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNSFLEYPIL GSGDSGTCSA RAYPSDHGIT TFQSCAVSAN SCGGDDRFLV
60 70 80 90 100
GRGVQISSPH HHHHHHHHHH PQTATYQTSG NLGISYSHSS CGPSYGAQNF
110 120 130 140 150
SAPYGPYGLN QEADVSGGYP PCAPAVYSGN LSTPMVQHHH HHQGYAGGTV
160 170 180 190 200
GSPQYIHHSY GQEQQTLALA TYNNSLSPLH ASHQEACRSP ASETSSPAQT
210 220 230 240 250
FDWMKVKRNP PKTGKVGEYG YVGQPNAVRT NFTTKQLTEL EKEFHFNKYL
260 270 280 290 300
TRARRVEIAA SLQLNETQVK IWFQNRRMKQ KKREKEGLLP ISPATPPGSD
310 320 330
EKTEESSEKS SPSPSAPSPA SSTSDTLTTS H
Length:331
Mass (Da):36,037
Last modified:April 1, 1990 - v2
Checksum:iF73E4FFB1C6F15B2
GO
Isoform ERA-1-399 (identifier: P09022-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-133: VSGGYPPCAPAVYSGNLST → PPRSLSFPCFRDVFSSADL
     134-331: Missing.

Show »
Length:133
Mass (Da):14,290
Checksum:iF3A12654DA61A299
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B9EHK7B9EHK7_MOUSE
Homeo box A1
Hoxa1 mCG_121168
336Annotation score:
D3Z4E1D3Z4E1_MOUSE
Homeobox protein Hox-A1
Hoxa1
138Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002380115 – 133VSGGY…GNLST → PPRSLSFPCFRDVFSSADL in isoform ERA-1-399. CuratedAdd BLAST19
Alternative sequenceiVSP_002381134 – 331Missing in isoform ERA-1-399. CuratedAdd BLAST198

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22115 mRNA Translation: AAA37559.1
M22115 mRNA Translation: AAA37558.1
X06023 Genomic DNA Translation: CAA29426.1
X06024 Genomic DNA Translation: CAA29427.1
M20214 Genomic DNA No translation available.
M20216, M20215 Genomic DNA Translation: AAA37839.1
PIRiA27155
A30242
B30242
UniGeneiMm.197

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22115 mRNA Translation: AAA37559.1
M22115 mRNA Translation: AAA37558.1
X06023 Genomic DNA Translation: CAA29426.1
X06024 Genomic DNA Translation: CAA29427.1
M20214 Genomic DNA No translation available.
M20216, M20215 Genomic DNA Translation: AAA37839.1
PIRiA27155
A30242
B30242
UniGeneiMm.197

3D structure databases

ProteinModelPortaliP09022
SMRiP09022
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59869N
IntActiP09022, 62 interactors
STRINGi10090.ENSMUSP00000000964

Proteomic databases

PaxDbiP09022
PRIDEiP09022

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:96170 Hoxa1

Phylogenomic databases

eggNOGiKOG0489 Eukaryota
ENOG410ZTBY LUCA
HOGENOMiHOG000171545
HOVERGENiHBG006089
InParanoidiP09022

Miscellaneous databases

PROiPR:P09022
SOURCEiSearch...

Gene expression databases

CleanExiMM_HOXA1

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR020479 Homeobox_metazoa
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PRINTSiPR00024 HOMEOBOX
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHXA1_MOUSE
AccessioniPrimary (citable) accession number: P09022
Secondary accession number(s): P15463
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 1, 1990
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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