UniProtKB - P08997 (MASY_ECOLI)
Protein
Malate synthase A
Gene
aceB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:2.3.3.9
: glyoxylate cycle Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Isocitrate lyase (aceA)
- Malate synthase G (glcB), Malate synthase G (glcB), Malate synthase (aceB), Malate synthase A (aceB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 166 | Proton acceptorBy similarity | 1 | |
Active sitei | 447 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- malate synthase activity Source: EcoCyc
GO - Biological processi
- glyoxylate cycle Source: GO_Central
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Enzyme and pathway databases
BioCyci | EcoCyc:MALATE-SYNTHASE MetaCyc:MALATE-SYNTHASE |
UniPathwayi | UPA00703;UER00720 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aceB Synonyms:mas Ordered Locus Names:b4014, JW3974 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: EcoliWiki
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166875 | 1 – 533 | Malate synthase AAdd BLAST | 533 |
Proteomic databases
jPOSTi | P08997 |
PaxDbi | P08997 |
PRIDEi | P08997 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259288, 6 interactors |
IntActi | P08997, 6 interactors |
STRINGi | 511145.b4014 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08997 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08997 |
Family & Domainsi
Sequence similaritiesi
Belongs to the malate synthase family.Curated
Phylogenomic databases
eggNOGi | COG2225, Bacteria |
HOGENOMi | CLU_018928_3_0_6 |
InParanoidi | P08997 |
PhylomeDBi | P08997 |
Family and domain databases
CDDi | cd00727, malate_synt_A, 1 hit |
InterProi | View protein in InterPro IPR011076, Malate_synth-like_sf IPR006252, Malate_synthA IPR001465, Malate_synthase IPR019830, Malate_synthase_CS |
PANTHERi | PTHR42902, PTHR42902, 1 hit |
Pfami | View protein in Pfam PF01274, Malate_synthase, 1 hit |
PIRSFi | PIRSF001363, Malate_synth, 1 hit |
SUPFAMi | SSF51645, SSF51645, 1 hit |
TIGRFAMsi | TIGR01344, malate_syn_A, 1 hit |
PROSITEi | View protein in PROSITE PS00510, MALATE_SYNTHASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P08997-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA
60 70 80 90 100
RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER
110 120 130 140 150
KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA
160 170 180 190 200
GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ
210 220 230 240 250
ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE
260 270 280 290 300
TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA
310 320 330 340 350
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK
360 370 380 390 400
ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI
410 420 430 440 450
TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT
460 470 480 490 500
AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS
510 520 530
QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12431 Genomic DNA Translation: CAA30973.1 U00006 Genomic DNA Translation: AAC43108.1 U00096 Genomic DNA Translation: AAC76984.1 AP009048 Genomic DNA Translation: BAE78016.1 |
PIRi | A32649, SYECMA |
RefSeqi | NP_418438.1, NC_000913.3 WP_000138905.1, NZ_SSZK01000049.1 |
Genome annotation databases
EnsemblBacteriai | AAC76984; AAC76984; b4014 BAE78016; BAE78016; BAE78016 |
GeneIDi | 948512 |
KEGGi | ecj:JW3974 eco:b4014 |
PATRICi | fig|1411691.4.peg.2699 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12431 Genomic DNA Translation: CAA30973.1 U00006 Genomic DNA Translation: AAC43108.1 U00096 Genomic DNA Translation: AAC76984.1 AP009048 Genomic DNA Translation: BAE78016.1 |
PIRi | A32649, SYECMA |
RefSeqi | NP_418438.1, NC_000913.3 WP_000138905.1, NZ_SSZK01000049.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3CUZ | X-ray | 1.04 | A | 2-533 | [»] | |
3CV1 | X-ray | 1.68 | A | 2-533 | [»] | |
3CV2 | X-ray | 1.40 | A/B | 2-533 | [»] | |
SMRi | P08997 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259288, 6 interactors |
IntActi | P08997, 6 interactors |
STRINGi | 511145.b4014 |
Proteomic databases
jPOSTi | P08997 |
PaxDbi | P08997 |
PRIDEi | P08997 |
Genome annotation databases
EnsemblBacteriai | AAC76984; AAC76984; b4014 BAE78016; BAE78016; BAE78016 |
GeneIDi | 948512 |
KEGGi | ecj:JW3974 eco:b4014 |
PATRICi | fig|1411691.4.peg.2699 |
Organism-specific databases
EchoBASEi | EB0022 |
Phylogenomic databases
eggNOGi | COG2225, Bacteria |
HOGENOMi | CLU_018928_3_0_6 |
InParanoidi | P08997 |
PhylomeDBi | P08997 |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00720 |
BioCyci | EcoCyc:MALATE-SYNTHASE MetaCyc:MALATE-SYNTHASE |
Miscellaneous databases
EvolutionaryTracei | P08997 |
PROi | PR:P08997 |
Family and domain databases
CDDi | cd00727, malate_synt_A, 1 hit |
InterProi | View protein in InterPro IPR011076, Malate_synth-like_sf IPR006252, Malate_synthA IPR001465, Malate_synthase IPR019830, Malate_synthase_CS |
PANTHERi | PTHR42902, PTHR42902, 1 hit |
Pfami | View protein in Pfam PF01274, Malate_synthase, 1 hit |
PIRSFi | PIRSF001363, Malate_synth, 1 hit |
SUPFAMi | SSF51645, SSF51645, 1 hit |
TIGRFAMsi | TIGR01344, malate_syn_A, 1 hit |
PROSITEi | View protein in PROSITE PS00510, MALATE_SYNTHASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MASY_ECOLI | |
Accessioni | P08997Primary (citable) accession number: P08997 Secondary accession number(s): Q2M6U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | November 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families