UniProtKB - P08997 (MASY_ECOLI)

BLAST

>sp|P08997|MASY_ECOLI Malate synthase A OS=Escherichia coli (strain K12) OX=83333 GN=aceB PE=1 SV=1
MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDN
GTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKMVINALNANVKVFMADFED
SLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPAVLICRVRGLHLPEKHVTWR
GEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQA
VTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFIPSKDEEHNNQVLNKVKADKSLEANNG
HDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPITADQLLAPCDGERTEEGMRA
NIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA

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History
Entry version 145 (07 Nov 2018)
Sequence version 1 (01 Nov 1988)
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Protein

Malate synthase A

Gene

aceB

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalytic activityⁱ

Acetyl-CoA + H₂O + glyoxylate = (S)-malate + CoA.

Pathwayⁱ: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Isocitrate lyase (aceA)
Malate synthase G (glcB), Malate synthase A (aceB)

This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	166	Proton acceptorBy similarity		1
Active siteⁱ	447	Proton donorBy similarity		1

GO - Molecular functionⁱ

malate synthase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 18714089

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glyoxylate cycle
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
tricarboxylic acid cycle Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCycⁱ	EcoCyc:MALATE-SYNTHASE MetaCyc:MALATE-SYNTHASE
UniPathwayⁱ	UPA00703;UER00720

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Malate synthase A (EC:2.3.3.9) Short name: MSA
Gene namesⁱ	Name:aceB Synonyms:mas Ordered Locus Names:b4014, JW3974
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10023 aceB

EcoGeneⁱ

EG10023 aceB

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytoplasm
Source: EcoliWiki
Inferred from direct assayⁱ
- 4874804

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000166875	1 – 533	Malate synthase AAdd BLAST		533

Proteomic databases

PaxDbⁱ	P08997
PRIDEⁱ	P08997

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4259288, 6 interactors
IntActⁱ	P08997, 6 interactors
STRINGⁱ	316385.ECDH10B_4203

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	12 – 15	Combined sources	4
Helixⁱ	19 – 24	Combined sources	6
Helixⁱ	27 – 59	Combined sources	33
Helixⁱ	71 – 74	Combined sources	4
Helixⁱ	85 – 87	Combined sources	3
Beta strandⁱ	91 – 97	Combined sources	7
Helixⁱ	100 – 107	Combined sources	8
Beta strandⁱ	109 – 119	Combined sources	11
Helixⁱ	126 – 140	Combined sources	15
Beta strandⁱ	145 – 147	Combined sources	3
Beta strandⁱ	153 – 155	Combined sources	3
Beta strandⁱ	162 – 166	Combined sources	5
Beta strandⁱ	173 – 179	Combined sources	7
Beta strandⁱ	182 – 185	Combined sources	4
Helixⁱ	186 – 204	Combined sources	19
Beta strandⁱ	210 – 213	Combined sources	4
Helixⁱ	219 – 235	Combined sources	17
Beta strandⁱ	243 – 248	Combined sources	6
Helixⁱ	252 – 255	Combined sources	4
Helixⁱ	258 – 264	Combined sources	7
Turnⁱ	265 – 268	Combined sources	4
Beta strandⁱ	269 – 273	Combined sources	5
Helixⁱ	277 – 286	Combined sources	10
Turnⁱ	287 – 289	Combined sources	3
Helixⁱ	291 – 293	Combined sources	3
Helixⁱ	298 – 300	Combined sources	3
Helixⁱ	306 – 321	Combined sources	16
Beta strandⁱ	325 – 331	Combined sources	7
Helixⁱ	339 – 341	Combined sources	3
Helixⁱ	343 – 359	Combined sources	17
Beta strandⁱ	362 – 368	Combined sources	7
Helixⁱ	369 – 371	Combined sources	3
Helixⁱ	372 – 383	Combined sources	12
Helixⁱ	402 – 406	Combined sources	5
Helixⁱ	415 – 433	Combined sources	19
Beta strandⁱ	438 – 441	Combined sources	4
Beta strandⁱ	444 – 446	Combined sources	3
Helixⁱ	448 – 463	Combined sources	16
Helixⁱ	476 – 494	Combined sources	19
Helixⁱ	496 – 501	Combined sources	6
Helixⁱ	504 – 516	Combined sources	13
Helixⁱ	525 – 529	Combined sources	5

Show more details

3D structure databases

ProteinModelPortalⁱ	P08997
SMRⁱ	P08997
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P08997

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4107QP3 Bacteria COG2225 LUCA
HOGENOMⁱ	HOG000238464
InParanoidⁱ	P08997
KEGG Orthology (KO) More...KOⁱ	K01638
PhylomeDBⁱ	P08997

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00727 malate_synt_A, 1 hit
InterProⁱ	View protein in InterPro IPR011076 Malate_synth-like_sf IPR006252 Malate_synthA IPR001465 Malate_synthase IPR019830 Malate_synthase_CS
PANTHERⁱ	PTHR42902 PTHR42902, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01274 Malate_synthase, 1 hit
PIRSFⁱ	PIRSF001363 Malate_synth, 1 hit
SUPFAMⁱ	SSF51645 SSF51645, 1 hit
TIGRFAMsⁱ	TIGR01344 malate_syn_A, 1 hit
PROSITEⁱ	View protein in PROSITE PS00510 MALATE_SYNTHASE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P08997-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA 
        60         70         80         90        100
RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER 
       110        120        130        140        150
KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA 
       160        170        180        190        200
GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ 
       210        220        230        240        250
ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE 
       260        270        280        290        300
TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA 
       310        320        330        340        350
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK 
       360        370        380        390        400
ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI 
       410        420        430        440        450
TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT 
       460        470        480        490        500
AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS 
       510        520        530 
QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA

Length:533

Mass (Da):60,274

Last modified:November 1, 1988 - v1

Checksum:ⁱEB88388D7E4C5098

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X12431 Genomic DNA Translation: CAA30973.1 U00006 Genomic DNA Translation: AAC43108.1 U00096 Genomic DNA Translation: AAC76984.1 AP009048 Genomic DNA Translation: BAE78016.1
PIRⁱ	A32649 SYECMA
NCBI Reference Sequences More...RefSeqⁱ	NP_418438.1, NC_000913.3 WP_000138905.1, NZ_CP014272.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76984; AAC76984; b4014 BAE78016; BAE78016; BAE78016
GeneIDⁱ	948512
KEGGⁱ	ecj:JW3974 eco:b4014
PATRICⁱ	fig\|1411691.4.peg.2699

Protein	Similar proteins		Species	Score	Length	Source
P08997	Malate synthase		Escherichia coli H736		539	UniRef100_P08997
Malate synthase		Escherichia coli H386		539
Malate synthase A		Shigella sp.		533
Malate synthase		ECOLX		533
Malate synthase		Escherichia coli HVH 50 (4-2593475)		533
+22

Protein	Similar proteins		Species	Score	Length	Source
P08997	Malate synthase		Escherichia coli H736		539	UniRef90_P08997
Malate synthase		Escherichia coli H386		539
Malate synthase A		Shigella sp.		533
Malate synthase		ECOLX		533
Malate synthase		Escherichia coli HVH 50 (4-2593475)		533
+233

Protein	Similar proteins		Species	Score	Length	Source
P08997	Malate synthase		ARATH		562	UniRef50_Q9LZC3
Malate synthase, glyoxysomal		BRANA		561
Malate synthase, glyoxysomal		GOSHI		567
Malate synthase, glyoxysomal		RICCO		567
Malate synthase, glyoxysomal		MAIZE		559
+5975

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X12431 Genomic DNA Translation: CAA30973.1 U00006 Genomic DNA Translation: AAC43108.1 U00096 Genomic DNA Translation: AAC76984.1 AP009048 Genomic DNA Translation: BAE78016.1
PIRⁱ	A32649 SYECMA
RefSeqⁱ	NP_418438.1, NC_000913.3 WP_000138905.1, NZ_CP014272.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3CUZ	X-ray	1.04	A	2-533	[»]
	3CV1	X-ray	1.68	A	2-533	[»]
	3CV2	X-ray	1.40	A/B	2-533	[»]
ProteinModelPortalⁱ	P08997
SMRⁱ	P08997
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259288, 6 interactors
IntActⁱ	P08997, 6 interactors
STRINGⁱ	316385.ECDH10B_4203

Proteomic databases

PaxDbⁱ	P08997
PRIDEⁱ	P08997

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76984; AAC76984; b4014 BAE78016; BAE78016; BAE78016
GeneIDⁱ	948512
KEGGⁱ	ecj:JW3974 eco:b4014
PATRICⁱ	fig\|1411691.4.peg.2699

Organism-specific databases

EchoBASEⁱ	EB0022
EcoGeneⁱ	EG10023 aceB

Phylogenomic databases

eggNOGⁱ	ENOG4107QP3 Bacteria COG2225 LUCA
HOGENOMⁱ	HOG000238464
InParanoidⁱ	P08997
KOⁱ	K01638
PhylomeDBⁱ	P08997

Enzyme and pathway databases

UniPathwayⁱ	UPA00703;UER00720
BioCycⁱ	EcoCyc:MALATE-SYNTHASE MetaCyc:MALATE-SYNTHASE

Miscellaneous databases

EvolutionaryTraceⁱ	P08997
Protein Ontology More...PROⁱ	PR:P08997

Family and domain databases

CDDⁱ	cd00727 malate_synt_A, 1 hit
InterProⁱ	View protein in InterPro IPR011076 Malate_synth-like_sf IPR006252 Malate_synthA IPR001465 Malate_synthase IPR019830 Malate_synthase_CS
PANTHERⁱ	PTHR42902 PTHR42902, 1 hit
Pfamⁱ	View protein in Pfam PF01274 Malate_synthase, 1 hit
PIRSFⁱ	PIRSF001363 Malate_synth, 1 hit
SUPFAMⁱ	SSF51645 SSF51645, 1 hit
TIGRFAMsⁱ	TIGR01344 malate_syn_A, 1 hit
PROSITEⁱ	View protein in PROSITE PS00510 MALATE_SYNTHASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MASY_ECOLI
Accessionⁱ	P08997Primary (citable) accession number: P08997 Secondary accession number(s): Q2M6U0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
	Last sequence update:	November 1, 1988
	Last modified:	November 7, 2018
	This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P08997 (MASY_ECOLI)

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Malate synthase A

aceB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glyoxylate cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

Pathwayⁱ: glyoxylate cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ