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Protein

Malate synthase A

Gene

aceB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (aceA)
  2. Malate synthase G (glcB), Malate synthase A (aceB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166Proton acceptorBy similarity1
Active sitei447Proton donorBy similarity1

GO - Molecular functioni

  • malate synthase activity Source: EcoCyc

GO - Biological processi

  • glyoxylate cycle Source: GO_Central
  • tricarboxylic acid cycle Source: UniProtKB-KW

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciEcoCyc:MALATE-SYNTHASE
MetaCyc:MALATE-SYNTHASE
UniPathwayi
UPA00703;UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase A (EC:2.3.3.9)
Short name:
MSA
Gene namesi
Name:aceB
Synonyms:mas
Ordered Locus Names:b4014, JW3974
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10023 aceB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668751 – 533Malate synthase AAdd BLAST533

Proteomic databases

PaxDbiP08997
PRIDEiP08997

Interactioni

Protein-protein interaction databases

BioGridi4259288, 6 interactors
IntActiP08997, 6 interactors
STRINGi316385.ECDH10B_4203

Structurei

Secondary structure

1533
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08997
SMRiP08997
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08997

Family & Domainsi

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000238464
InParanoidiP08997
KOiK01638
PhylomeDBiP08997

Family and domain databases

CDDicd00727 malate_synt_A, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR006252 Malate_synthA
IPR001465 Malate_synthase
IPR019830 Malate_synthase_CS
PANTHERiPTHR42902 PTHR42902, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
PIRSFiPIRSF001363 Malate_synth, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01344 malate_syn_A, 1 hit
PROSITEiView protein in PROSITE
PS00510 MALATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P08997-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA
60 70 80 90 100
RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER
110 120 130 140 150
KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA
160 170 180 190 200
GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ
210 220 230 240 250
ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE
260 270 280 290 300
TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA
310 320 330 340 350
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK
360 370 380 390 400
ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI
410 420 430 440 450
TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT
460 470 480 490 500
AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS
510 520 530
QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
Length:533
Mass (Da):60,274
Last modified:November 1, 1988 - v1
Checksum:iEB88388D7E4C5098
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12431 Genomic DNA Translation: CAA30973.1
U00006 Genomic DNA Translation: AAC43108.1
U00096 Genomic DNA Translation: AAC76984.1
AP009048 Genomic DNA Translation: BAE78016.1
PIRiA32649 SYECMA
RefSeqiNP_418438.1, NC_000913.3
WP_000138905.1, NZ_CP014272.1

Genome annotation databases

EnsemblBacteriaiAAC76984; AAC76984; b4014
BAE78016; BAE78016; BAE78016
GeneIDi948512
KEGGiecj:JW3974
eco:b4014
PATRICifig|1411691.4.peg.2699

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12431 Genomic DNA Translation: CAA30973.1
U00006 Genomic DNA Translation: AAC43108.1
U00096 Genomic DNA Translation: AAC76984.1
AP009048 Genomic DNA Translation: BAE78016.1
PIRiA32649 SYECMA
RefSeqiNP_418438.1, NC_000913.3
WP_000138905.1, NZ_CP014272.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CUZX-ray1.04A2-533[»]
3CV1X-ray1.68A2-533[»]
3CV2X-ray1.40A/B2-533[»]
ProteinModelPortaliP08997
SMRiP08997
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259288, 6 interactors
IntActiP08997, 6 interactors
STRINGi316385.ECDH10B_4203

Proteomic databases

PaxDbiP08997
PRIDEiP08997

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76984; AAC76984; b4014
BAE78016; BAE78016; BAE78016
GeneIDi948512
KEGGiecj:JW3974
eco:b4014
PATRICifig|1411691.4.peg.2699

Organism-specific databases

EchoBASEiEB0022
EcoGeneiEG10023 aceB

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000238464
InParanoidiP08997
KOiK01638
PhylomeDBiP08997

Enzyme and pathway databases

UniPathwayi
UPA00703;UER00720

BioCyciEcoCyc:MALATE-SYNTHASE
MetaCyc:MALATE-SYNTHASE

Miscellaneous databases

EvolutionaryTraceiP08997
PROiPR:P08997

Family and domain databases

CDDicd00727 malate_synt_A, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR006252 Malate_synthA
IPR001465 Malate_synthase
IPR019830 Malate_synthase_CS
PANTHERiPTHR42902 PTHR42902, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
PIRSFiPIRSF001363 Malate_synth, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01344 malate_syn_A, 1 hit
PROSITEiView protein in PROSITE
PS00510 MALATE_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMASY_ECOLI
AccessioniPrimary (citable) accession number: P08997
Secondary accession number(s): Q2M6U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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