UniProtKB - P08970 (SUHW_DROME)
Protein
Protein suppressor of hairy wing
Gene
su(Hw)
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein binds specifically to a region of the gypsy element located 3' of the 5' long terminal repeat (LTR), and may also mediate interaction with other endogenous insulators at sites distinct from those recognized by Cp190.8 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 220 – 242 | C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 290 – 313 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 319 – 341 | C2H2-type 3; atypicalPROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 348 – 366 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 19 | |
Zinc fingeri | 380 – 402 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 413 – 435 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 441 – 463 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 469 – 491 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 497 – 519 | C2H2-type 9PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 523 – 545 | C2H2-type 10PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 553 – 577 | C2H2-type 11PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 596 – 619 | C2H2-type 12PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- chromatin insulator sequence binding Source: FlyBase
- DNA binding Source: FlyBase
- DNA-binding transcription factor activity Source: GO_Central
- euchromatin binding Source: FlyBase
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: FlyBase
GO - Biological processi
- chromatin organization Source: UniProtKB-KW
- negative regulation of chromatin silencing Source: FlyBase
- negative regulation of transcription, DNA-templated Source: UniProtKB
- protein localization to euchromatin Source: FlyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to ecdysone Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
SignaLinki | P08970 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein suppressor of hairy wing |
Gene namesi | Name:su(Hw) ORF Names:CG8573 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003567, su(Hw) |
Subcellular locationi
Nucleus
Other locations
Note: Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies.
Nucleus
- nuclear chromatin Source: GO_Central
- nucleus Source: UniProtKB
Other locations
- chromatin Source: FlyBase
- polytene chromosome Source: FlyBase
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047053 | 1 – 941 | Protein suppressor of hairy wingAdd BLAST | 941 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 186 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P08970 |
PRIDEi | P08970 |
PTM databases
iPTMneti | P08970 |
Expressioni
Developmental stagei
Expressed in all stages of development.1 Publication
Gene expression databases
Bgeei | FBgn0003567, Expressed in embryo and 75 other tissues |
ExpressionAtlasi | P08970, baseline and differential |
Genevisiblei | P08970, DM |
Interactioni
Subunit structurei
Component of the gypsy chromatin insulator complex, composed of Cp190, mod(mdg4) and su(Hw) (PubMed:7664338, PubMed:11350941, PubMed:11416154, PubMed:15574329). The gypsy chromatin insulator complex interacts with Topors via mod(mdg4) and su(Hw) (PubMed:16209949). Upon ecdysone stimulation, interacts with Nup98 (PubMed:28366641).
6 PublicationsBinary interactionsi
P08970
With | #Exp. | IntAct |
---|---|---|
Cp190 [Q24478] | 4 | EBI-101373,EBI-868840 |
Protein-protein interaction databases
BioGRIDi | 66816, 53 interactors |
DIPi | DIP-17895N |
IntActi | P08970, 21 interactors |
MINTi | P08970 |
STRINGi | 7227.FBpp0082404 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 760 – 860 | Interaction with mod(mdg4)Add BLAST | 101 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 155 – 202 | Asp/Glu-rich (acidic)Add BLAST | 48 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 220 – 242 | C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 290 – 313 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 319 – 341 | C2H2-type 3; atypicalPROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 348 – 366 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 19 | |
Zinc fingeri | 380 – 402 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 413 – 435 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 441 – 463 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 469 – 491 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 497 – 519 | C2H2-type 9PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 523 – 545 | C2H2-type 10PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 553 – 577 | C2H2-type 11PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 596 – 619 | C2H2-type 12PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000166978 |
HOGENOMi | CLU_013489_0_0_1 |
InParanoidi | P08970 |
OMAi | KMDHYVL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P08970 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 6 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 12 hits |
SUPFAMi | SSF57667, SSF57667, 5 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 10 hits PS50157, ZINC_FINGER_C2H2_2, 10 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P08970-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSASKEGKEK KGKLLGVENI SPPKDKRPAT RMKLLNDVGA GEDSEASTTT
60 70 80 90 100
TTSRTPSNKQ EKRGSVAGSR IKILNEEILG TPKTEKRGAT KSTAPAASTV
110 120 130 140 150
KILNEKKTPS ATVTAVETTK IKTSPSKRKK MEHYVLQAVK SENTKADTTV
160 170 180 190 200
TVVTEEDDTI DFILADDEEV VPGRIENNNG QEIVVTEDDE DLGEDGDEDG
210 220 230 240 250
EDSSGKGNSS QTKIKEIVEH VCGKCYKTFR RVQSLKKHLE FCRYDSGYHL
260 270 280 290 300
RKADMLKNLE KIEKDAVVME KKDICFCCSE SYDTFHLGHI NCPDCPKSFK
310 320 330 340 350
TQTSYERHIF ITHSEFSDFP CSICNANLRS EALLALHEEQ HKSRGKPYAC
360 370 380 390 400
KICGKDFTRS YHLKRHQKYS SCSSNETDTM SCKVCDRVFY RLDNLRSHLK
410 420 430 440 450
QHLGTQVVKK PEYMCHTCKN CFYSLSTLNI HIRTHTGEKP FDCDLCDKKF
460 470 480 490 500
SALVALKKHR RYHTGEKPYS CTVCNQAFAV KEVLNRHMKR HTGERPHKCD
510 520 530 540 550
ECGKSFIQAT QLRTHSKTHI RPFPCEQCDE KFKTEKQLER HVKTHSRTKR
560 570 580 590 600
PVFSCAECKR NFRTPALLKE HMDEGKHSPK QQRSSMRSAV KIMERTDCAI
610 620 630 640 650
CDKNFDSSDT LRRHIRTVHE CDPDDIFGVE PHPSKRAKKD IESEEVVPVA
660 670 680 690 700
LNTSAGSLIS SQTDGNGVVV REFLVDEGDG AAQTITLENE TYTILPLDGA
710 720 730 740 750
IEGEQLTDEA GVKPEAKKEE AQVSPVVKKE QRKSLAASLA AAIADNLEES
760 770 780 790 800
CSEDDFSGEI LTEEDIKLKE NVGKLIDMLV DPPILKKYGW PNAPEETVLC
810 820 830 840 850
KVIENCGHDL TKGGENYAEL DYGSRMREYC KLLFTVVIHN DSIKSLLNNF
860 870 880 890 900
PIDDVIEYVL GDEDQDEGGL DKDNESHSGD EEAVSVTGET KTNEIREKPE
910 920 930 940
KKEVSAKSEK KEIVGKAVDK DNSEEVVREN KKKPVGEQEK A
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4K6W1 | A0A0B4K6W1_DROME | Suppressor of hairy wing, isoform C | su(Hw) Dmel\CG8573, SU(HW), Su(HW), Su(Hw), Su(hw) | 941 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | R → K in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 61 | E → G in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 64 | G → D in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 68 | G → E in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 249 | H → R in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 347 | P → S in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 508 | Q → R in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 667 | G → D in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 874 | N → NEDN in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 892 | T → K in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 896 | R → K in CAA68371 (PubMed:2462523).Curated | 1 | |
Sequence conflicti | 923 | S → T in CAA68371 (PubMed:2462523).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00228 Genomic DNA Translation: CAA68371.1 AE014297 Genomic DNA Translation: AAF55044.1 BT003273 mRNA Translation: AAO25030.1 |
PIRi | S01909 |
RefSeqi | NP_001247098.1, NM_001260169.2 NP_524349.1, NM_079625.3 NP_731897.1, NM_169574.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0082945; FBpp0082404; FBgn0003567 FBtr0082946; FBpp0082405; FBgn0003567 FBtr0310002; FBpp0301708; FBgn0003567 |
GeneIDi | 41740 |
KEGGi | dme:Dmel_CG8573 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00228 Genomic DNA Translation: CAA68371.1 AE014297 Genomic DNA Translation: AAF55044.1 BT003273 mRNA Translation: AAO25030.1 |
PIRi | S01909 |
RefSeqi | NP_001247098.1, NM_001260169.2 NP_524349.1, NM_079625.3 NP_731897.1, NM_169574.2 |
3D structure databases
SMRi | P08970 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 66816, 53 interactors |
DIPi | DIP-17895N |
IntActi | P08970, 21 interactors |
MINTi | P08970 |
STRINGi | 7227.FBpp0082404 |
PTM databases
iPTMneti | P08970 |
Proteomic databases
PaxDbi | P08970 |
PRIDEi | P08970 |
Genome annotation databases
EnsemblMetazoai | FBtr0082945; FBpp0082404; FBgn0003567 FBtr0082946; FBpp0082405; FBgn0003567 FBtr0310002; FBpp0301708; FBgn0003567 |
GeneIDi | 41740 |
KEGGi | dme:Dmel_CG8573 |
Organism-specific databases
CTDi | 41740 |
FlyBasei | FBgn0003567, su(Hw) |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000166978 |
HOGENOMi | CLU_013489_0_0_1 |
InParanoidi | P08970 |
OMAi | KMDHYVL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P08970 |
Enzyme and pathway databases
SignaLinki | P08970 |
Miscellaneous databases
BioGRID-ORCSi | 41740, 0 hits in 1 CRISPR screen |
ChiTaRSi | su(Hw), fly |
GenomeRNAii | 41740 |
PROi | PR:P08970 |
Gene expression databases
Bgeei | FBgn0003567, Expressed in embryo and 75 other tissues |
ExpressionAtlasi | P08970, baseline and differential |
Genevisiblei | P08970, DM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 6 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 12 hits |
SUPFAMi | SSF57667, SSF57667, 5 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 10 hits PS50157, ZINC_FINGER_C2H2_2, 10 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUHW_DROME | |
Accessioni | P08970Primary (citable) accession number: P08970 Secondary accession number(s): Q0KI74, Q9VFK9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | August 15, 2003 | |
Last modified: | February 10, 2021 | |
This is version 196 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase