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Protein

CD63 antigen

Gene

CD63

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.5 Publications

Miscellaneous

Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.
This antigen is associated with early stages of melanoma tumor progression.

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation
R-HSA-6798695 Neutrophil degranulation

Protein family/group databases

TCDBi8.A.40.1.19 the tetraspanin (tetraspanin) family

Names & Taxonomyi

Protein namesi
Recommended name:
CD63 antigen
Alternative name(s):
Granulophysin
Lysosomal-associated membrane protein 3
Short name:
LAMP-3
Melanoma-associated antigen ME491
OMA81H
Ocular melanoma-associated antigen
Tetraspanin-30
Short name:
Tspan-30
CD_antigen: CD63
Gene namesi
Name:CD63
Synonyms:MLA1, TSPAN30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000135404.11
HGNCiHGNC:1692 CD63
MIMi155740 gene
neXtProtiNX_P08962

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 11CytoplasmicSequence analysis10
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 51ExtracellularSequence analysisAdd BLAST19
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Topological domaini73 – 81CytoplasmicSequence analysis9
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 203ExtracellularSequence analysisAdd BLAST101
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Topological domaini225 – 238CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi967
OpenTargetsiENSG00000135404
PharmGKBiPA26231

Chemistry databases

ChEMBLiCHEMBL3713303

Polymorphism and mutation databases

BioMutaiCD63
DMDMi116026

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002192162 – 238CD63 antigenAdd BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi130N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi150N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

EPDiP08962
MaxQBiP08962
PaxDbiP08962
PeptideAtlasiP08962
PRIDEiP08962
ProteomicsDBi52180

PTM databases

GlyConnecti1088
iPTMnetiP08962
PhosphoSitePlusiP08962
SwissPalmiP08962

Expressioni

Tissue specificityi

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.1 Publication

Gene expression databases

BgeeiENSG00000135404 Expressed in 233 organ(s), highest expression level in upper lobe of lung
CleanExiHS_CD63
ExpressionAtlasiP08962 baseline and differential
GenevisibleiP08962 HS

Organism-specific databases

HPAiCAB026356
HPA010088

Interactioni

Subunit structurei

Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1 (PubMed:16917503, PubMed:24635319). Interacts with CD9. Identified in a complex with CD9 and ITGB3 (PubMed:19640571). Interacts with PMEL (PubMed:21962903). Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes (PubMed:23632027). Interacts with SYT7 (By similarity).By similarity5 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi107405, 37 interactors
CORUMiP08962
IntActiP08962, 20 interactors
MINTiP08962
STRINGi9606.ENSP00000257857

Chemistry databases

BindingDBiP08962

Structurei

3D structure databases

ProteinModelPortaliP08962
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi234 – 238Lysosomal targeting motifBy similarity5

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3882 Eukaryota
ENOG4111IRY LUCA
GeneTreeiENSGT00880000137851
HOGENOMiHOG000230651
HOVERGENiHBG107306
InParanoidiP08962
KOiK06497
OMAiNDMISHY
OrthoDBiEOG091G12WV
PhylomeDBiP08962
TreeFamiTF316345

Family and domain databases

Gene3Di1.10.1450.10, 1 hit
InterProiView protein in InterPro
IPR000301 Tetraspanin
IPR018499 Tetraspanin/Peripherin
IPR018503 Tetraspanin_CS
IPR008952 Tetraspanin_EC2_sf
PfamiView protein in Pfam
PF00335 Tetraspannin, 1 hit
PIRSFiPIRSF002419 Tetraspanin, 1 hit
PRINTSiPR00259 TMFOUR
SUPFAMiSSF48652 SSF48652, 1 hit
PROSITEiView protein in PROSITE
PS00421 TM4_1, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P08962-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAVEGGMKCV KFLLYVLLLA FCACAVGLIA VGVGAQLVLS QTIIQGATPG
60 70 80 90 100
SLLPVVIIAV GVFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAA
110 120 130 140 150
AIAGYVFRDK VMSEFNNNFR QQMENYPKNN HTASILDRMQ ADFKCCGAAN
160 170 180 190 200
YTDWEKIPSM SKNRVPDSCC INVTVGCGIN FNEKAIHKEG CVEKIGGWLR
210 220 230
KNVLVVAAAA LGIAFVEVLG IVFACCLVKS IRSGYEVM
Length:238
Mass (Da):25,637
Last modified:January 23, 2007 - v2
Checksum:i85AC8E235C6E425F
GO
Isoform 2 (identifier: P08962-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     23-45: Missing.

Show »
Length:215
Mass (Da):23,430
Checksum:iC4469E42BCC8C4F7
GO
Isoform 3 (identifier: P08962-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: Gene prediction based on EST data.
Show »
Length:156
Mass (Da):17,271
Checksum:iD5A714579CDE49EF
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8W022F8W022_HUMAN
Tetraspanin
CD63
215Annotation score:
F8VWK8F8VWK8_HUMAN
Tetraspanin
CD63
217Annotation score:
F8VNT9F8VNT9_HUMAN
CD63 antigen
CD63
133Annotation score:
F8VV56F8VV56_HUMAN
CD63 antigen
CD63
145Annotation score:
F8VX62F8VX62_HUMAN
CD63 antigen
CD63
110Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36Q → E AA sequence (PubMed:7682577).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0469961 – 82Missing in isoform 3. CuratedAdd BLAST82
Alternative sequenceiVSP_04530023 – 45Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07982 mRNA Translation: CAA30792.1
M59907 mRNA Translation: AAA63235.1
M58485 mRNA No translation available.
S93788 mRNA Translation: AAB21617.1
X62654 Genomic DNA Translation: CAA44519.1
AF508304 mRNA Translation: AAM34259.1
AK311893 mRNA Translation: BAG34834.1
CR542096 mRNA Translation: CAG46893.1
BT007073 mRNA Translation: AAP35736.1
BT020137 mRNA Translation: AAV38939.1
BT020138 mRNA Translation: AAV38940.1
AC009779 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW96827.1
BC002349 mRNA Translation: AAH02349.1
BC013017 mRNA Translation: AAH13017.1
CCDSiCCDS58242.1 [P08962-3]
CCDS58243.1 [P08962-2]
CCDS8890.1 [P08962-1]
PIRiI38016
RefSeqiNP_001244318.1, NM_001257389.1 [P08962-1]
NP_001244319.1, NM_001257390.1 [P08962-1]
NP_001244320.1, NM_001257391.1 [P08962-1]
NP_001244321.1, NM_001257392.1 [P08962-2]
NP_001244329.1, NM_001257400.1 [P08962-3]
NP_001244330.1, NM_001257401.1 [P08962-3]
NP_001254627.1, NM_001267698.1 [P08962-1]
NP_001771.1, NM_001780.5 [P08962-1]
UniGeneiHs.445570

Genome annotation databases

EnsembliENST00000257857; ENSP00000257857; ENSG00000135404 [P08962-1]
ENST00000420846; ENSP00000393502; ENSG00000135404 [P08962-1]
ENST00000546939; ENSP00000447356; ENSG00000135404 [P08962-3]
ENST00000549117; ENSP00000447730; ENSG00000135404 [P08962-1]
ENST00000550776; ENSP00000448091; ENSG00000135404 [P08962-3]
ENST00000552692; ENSP00000449337; ENSG00000135404 [P08962-1]
ENST00000552754; ENSP00000446807; ENSG00000135404 [P08962-2]
GeneIDi967
KEGGihsa:967
UCSCiuc001shn.5 human [P08962-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07982 mRNA Translation: CAA30792.1
M59907 mRNA Translation: AAA63235.1
M58485 mRNA No translation available.
S93788 mRNA Translation: AAB21617.1
X62654 Genomic DNA Translation: CAA44519.1
AF508304 mRNA Translation: AAM34259.1
AK311893 mRNA Translation: BAG34834.1
CR542096 mRNA Translation: CAG46893.1
BT007073 mRNA Translation: AAP35736.1
BT020137 mRNA Translation: AAV38939.1
BT020138 mRNA Translation: AAV38940.1
AC009779 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW96827.1
BC002349 mRNA Translation: AAH02349.1
BC013017 mRNA Translation: AAH13017.1
CCDSiCCDS58242.1 [P08962-3]
CCDS58243.1 [P08962-2]
CCDS8890.1 [P08962-1]
PIRiI38016
RefSeqiNP_001244318.1, NM_001257389.1 [P08962-1]
NP_001244319.1, NM_001257390.1 [P08962-1]
NP_001244320.1, NM_001257391.1 [P08962-1]
NP_001244321.1, NM_001257392.1 [P08962-2]
NP_001244329.1, NM_001257400.1 [P08962-3]
NP_001244330.1, NM_001257401.1 [P08962-3]
NP_001254627.1, NM_001267698.1 [P08962-1]
NP_001771.1, NM_001780.5 [P08962-1]
UniGeneiHs.445570

3D structure databases

ProteinModelPortaliP08962
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107405, 37 interactors
CORUMiP08962
IntActiP08962, 20 interactors
MINTiP08962
STRINGi9606.ENSP00000257857

Chemistry databases

BindingDBiP08962
ChEMBLiCHEMBL3713303

Protein family/group databases

TCDBi8.A.40.1.19 the tetraspanin (tetraspanin) family

PTM databases

GlyConnecti1088
iPTMnetiP08962
PhosphoSitePlusiP08962
SwissPalmiP08962

Polymorphism and mutation databases

BioMutaiCD63
DMDMi116026

Proteomic databases

EPDiP08962
MaxQBiP08962
PaxDbiP08962
PeptideAtlasiP08962
PRIDEiP08962
ProteomicsDBi52180

Protocols and materials databases

DNASUi967
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257857; ENSP00000257857; ENSG00000135404 [P08962-1]
ENST00000420846; ENSP00000393502; ENSG00000135404 [P08962-1]
ENST00000546939; ENSP00000447356; ENSG00000135404 [P08962-3]
ENST00000549117; ENSP00000447730; ENSG00000135404 [P08962-1]
ENST00000550776; ENSP00000448091; ENSG00000135404 [P08962-3]
ENST00000552692; ENSP00000449337; ENSG00000135404 [P08962-1]
ENST00000552754; ENSP00000446807; ENSG00000135404 [P08962-2]
GeneIDi967
KEGGihsa:967
UCSCiuc001shn.5 human [P08962-1]

Organism-specific databases

CTDi967
DisGeNETi967
EuPathDBiHostDB:ENSG00000135404.11
GeneCardsiCD63
HGNCiHGNC:1692 CD63
HPAiCAB026356
HPA010088
MIMi155740 gene
neXtProtiNX_P08962
OpenTargetsiENSG00000135404
PharmGKBiPA26231
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3882 Eukaryota
ENOG4111IRY LUCA
GeneTreeiENSGT00880000137851
HOGENOMiHOG000230651
HOVERGENiHBG107306
InParanoidiP08962
KOiK06497
OMAiNDMISHY
OrthoDBiEOG091G12WV
PhylomeDBiP08962
TreeFamiTF316345

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation
R-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiCD63 human
GeneWikiiCD63
GenomeRNAii967
PROiPR:P08962
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135404 Expressed in 233 organ(s), highest expression level in upper lobe of lung
CleanExiHS_CD63
ExpressionAtlasiP08962 baseline and differential
GenevisibleiP08962 HS

Family and domain databases

Gene3Di1.10.1450.10, 1 hit
InterProiView protein in InterPro
IPR000301 Tetraspanin
IPR018499 Tetraspanin/Peripherin
IPR018503 Tetraspanin_CS
IPR008952 Tetraspanin_EC2_sf
PfamiView protein in Pfam
PF00335 Tetraspannin, 1 hit
PIRSFiPIRSF002419 Tetraspanin, 1 hit
PRINTSiPR00259 TMFOUR
SUPFAMiSSF48652 SSF48652, 1 hit
PROSITEiView protein in PROSITE
PS00421 TM4_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCD63_HUMAN
AccessioniPrimary (citable) accession number: P08962
Secondary accession number(s): F8VZE2
, Q5TZP3, Q8N6Z9, Q9UCG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 191 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
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Main funding by: National Institutes of Health

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