UniProtKB - P08934 (KNG1_RAT)
Kininogen-1
Kng1
Functioni
1 Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
Miscellaneous
GO - Molecular functioni
- cysteine-type endopeptidase inhibitor activity Source: RGD
- endopeptidase inhibitor activity Source: GO_Central
- protease binding Source: RGD
- signaling receptor binding Source: RGD
GO - Biological processi
- blood coagulation Source: UniProtKB-KW
- Factor XII activation Source: RGD
- inflammatory response Source: RGD
- negative regulation of blood coagulation Source: RGD
- negative regulation of cell adhesion Source: RGD
- negative regulation of endopeptidase activity Source: GO_Central
- negative regulation of lymphocyte proliferation Source: RGD
- negative regulation of MAP kinase activity Source: RGD
- negative regulation of proteolysis Source: RGD
- negative regulation of T cell proliferation Source: RGD
- positive regulation of cytosolic calcium ion concentration Source: RGD
- positive regulation of endothelial cell proliferation Source: RGD
- positive regulation of fibroblast proliferation Source: RGD
- positive regulation of MAPK cascade Source: RGD
- regulation of endopeptidase activity Source: RGD
- vasodilation Source: UniProtKB-KW
Keywordsi
Molecular function | Protease inhibitor, Thiol protease inhibitor, Vasoactive, Vasodilator |
Biological process | Blood coagulation, Hemostasis, Inflammatory response |
Protein family/group databases
MEROPSi | I25.018 |
Names & Taxonomyi
Protein namesi | Recommended name: Kininogen-1Cleaved into the following 3 chains: |
Gene namesi | Name:Kng1 Synonyms:Kng |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2980, Kng1 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: GO_Central
- extracellular space Source: RGD
Other locations
- intracellular membrane-bounded organelle Source: RGD
- perikaryon Source: RGD
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Add BLAST | 18 | |
ChainiPRO_0000006695 | 19 – 639 | Kininogen-1Add BLAST | 621 | |
ChainiPRO_0000006696 | 19 – 380 | Kininogen-1 heavy chainAdd BLAST | 362 | |
PeptideiPRO_0000006697 | 381 – 389 | Bradykinin | 9 | |
ChainiPRO_0000006698 | 390 – 639 | Kininogen-1 light chainAdd BLAST | 250 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 28 ↔ 609 | Interchain (between heavy and light chains)PROSITE-ProRule annotation | ||
Glycosylationi | 82 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 83 ↔ 94 | PROSITE-ProRule annotation | ||
Disulfide bondi | 107 ↔ 126 | PROSITE-ProRule annotation | ||
Disulfide bondi | 142 ↔ 145 | PROSITE-ProRule annotation | ||
Glycosylationi | 169 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 205 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 206 ↔ 218 | PROSITE-ProRule annotation | ||
Disulfide bondi | 229 ↔ 248 | PROSITE-ProRule annotation | ||
Disulfide bondi | 264 ↔ 267 | PROSITE-ProRule annotation | ||
Glycosylationi | 294 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 328 ↔ 340 | PROSITE-ProRule annotation | ||
Modified residuei | 332 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 351 ↔ 370 | PROSITE-ProRule annotation | ||
Glycosylationi | 529 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | P08934 |
PaxDbi | P08934 |
PRIDEi | P08934 |
PTM databases
GlyGeni | P08934, 5 sites |
PhosphoSitePlusi | P08934 |
Expressioni
Tissue specificityi
Interactioni
GO - Molecular functioni
- protease binding Source: RGD
- signaling receptor binding Source: RGD
Protein-protein interaction databases
IntActi | P08934, 1 interactor |
STRINGi | 10116.ENSRNOP00000065146 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 132 | Cystatin kininogen-type 1PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 151 – 254 | Cystatin kininogen-type 2PROSITE-ProRule annotationAdd BLAST | 104 | |
Domaini | 273 – 376 | Cystatin kininogen-type 3PROSITE-ProRule annotationAdd BLAST | 104 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 438 – 462 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 476 – 547 | DisorderedSequence analysisAdd BLAST | 72 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 438 – 459 | Basic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 484 – 500 | Basic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 501 – 516 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 517 – 543 | Polar residuesSequence analysisAdd BLAST | 27 |
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502RYAC, Eukaryota |
InParanoidi | P08934 |
Family and domain databases
CDDi | cd00042, CY, 3 hits |
InterProi | View protein in InterPro IPR000010, Cystatin_dom IPR046350, Cystatin_sf IPR002395, Kininogen IPR027358, Kininogen-type_cystatin_dom IPR018073, Prot_inh_cystat_CS |
Pfami | View protein in Pfam PF00031, Cystatin, 3 hits |
PRINTSi | PR00334, KININOGEN |
SMARTi | View protein in SMART SM00043, CY, 3 hits |
SUPFAMi | SSF54403, SSF54403, 3 hits |
PROSITEi | View protein in PROSITE PS00287, CYSTATIN, 2 hits PS51647, CYSTATIN_KININOGEN, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKLITILLLC SRLLPSLAQE EDAQEMDCND ESLFQAVDTA LKKYNAGLKS
60 70 80 90 100
GNQFVLYQVT EGTKKDGSKT FYSFKYQIKE GNCSVQSGFA WQDCDFKDAE
110 120 130 140 150
EAATGECTAT LEKRRNNKFS IATQICNITP GKGPIVTNEY HCLGCMHPIS
160 170 180 190 200
VDSPELGPVL KHAVEHFNNN TKHTHLFALG EVKSADRQVV AGMNYQIIYS
210 220 230 240 250
IVQTNCSKED FPSLHEDCVP LPSGDDGECK GNAFVDIHKT IAGFSDSCEF
260 270 280 290 300
YPGDDLFELL PEDCPGCPRN IPVDSPELKE ALGHSIAQLN AENNHTFYFK
310 320 330 340 350
IDTVKKATSQ VVAGTKYVIE FIARETKCSK ESNAELTADC ETKRLGQSLN
360 370 380 390 400
CNANVYMRPW ENKVVPTVKC KVLDMTSVIR RPPGFSPFRA PRVKKPKEST
410 420 430 440 450
TVSPSYIARV QEERDPGNEQ GPIHGHGWLH AKQIKNKNHQ GHKHGHGIGH
460 470 480 490 500
GHQKPHGLGH GHQLKLDDLK QQREDGYDHR HPVGHGHGQR HGHGHGHGHG
510 520 530 540 550
RDKHTNKDKN NVKHTDQRRA PLTSSSEDNT TSTQIQGRTE GFTLNPPLAQ
560 570 580 590 600
PAVISRGFQD SGFTEGVIAT TSPYDTETHD DLIPDIHVQP DSLSFKLISD
610 620 630
FPEATSHKCP GRPWKPVSRK DPTIETTEFS DFDLLDALS
The sequence of this isoform differs from the canonical sequence as follows:
402-433: VSPSYIARVQEERDPGNEQGPIHGHGWLHAKQ → RLLNSCEYKGRLLKAGAGPAPERQAEASTVTP
434-639: Missing.
Computationally mapped potential isoform sequencesi
There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5PQU1 | Q5PQU1_RAT | Kininogen 1 | Kng2 Kng1, Kng1l1 | 430 | Annotation score: | ||
A0A0G2JVQ5 | A0A0G2JVQ5_RAT | Kininogen 2 | Kng1 Kng1l1, Kng2 | 677 | Annotation score: | ||
A0A0G2KA54 | A0A0G2KA54_RAT | Kininogen-1 | Kng1 Kng1l1, Kng2 | 659 | Annotation score: | ||
F7EUK4 | F7EUK4_RAT | Kininogen-1 | Kng1 Kng1l1, Kng2 | 421 | Annotation score: | ||
A0A8I6GER1 | A0A8I6GER1_RAT | Kininogen 2 | Kng2 rCG_36716 | 639 | Annotation score: | ||
A0A8I5Y6S4 | A0A8I5Y6S4_RAT | Kininogen 2 | Kng2 | 430 | Annotation score: | ||
A0A8I5ZK39 | A0A8I5ZK39_RAT | Kininogen 2 | Kng2 | 420 | Annotation score: | ||
A0A8I5ZRM0 | A0A8I5ZRM0_RAT | Kininogen 2 | Kng2 | 638 | Annotation score: | ||
A0A8I5ZWQ0 | A0A8I5ZWQ0_RAT | Kininogen 2 | Kng2 | 625 | Annotation score: | ||
A0A8I6ACG6 | A0A8I6ACG6_RAT | Kininogen 2 | Kng2 | 458 | Annotation score: | ||
There is more potential isoformShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | E → K (PubMed:2413018).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001265 | 402 – 433 | VSPSY…LHAKQ → RLLNSCEYKGRLLKAGAGPA PERQAEASTVTP in isoform LMW. 2 PublicationsAdd BLAST | 32 | |
Alternative sequenceiVSP_001266 | 434 – 639 | Missing in isoform LMW. 2 PublicationsAdd BLAST | 206 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L29428 mRNA Translation: AAA41486.1 M11884 mRNA Translation: AAA41487.1 M14369 Genomic DNA Translation: AAA41484.1 M14369 Genomic DNA Translation: AAA41485.1 Sequence problems. M16455 Genomic DNA Translation: AAA41482.1 |
PIRi | A25486 A28055 C25486 |
RefSeqi | NP_036873.1, NM_012741.1 [P08934-1] |
Genome annotation databases
GeneIDi | 25087 |
KEGGi | rno:25087 |
UCSCi | RGD:2980, rat [P08934-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L29428 mRNA Translation: AAA41486.1 M11884 mRNA Translation: AAA41487.1 M14369 Genomic DNA Translation: AAA41484.1 M14369 Genomic DNA Translation: AAA41485.1 Sequence problems. M16455 Genomic DNA Translation: AAA41482.1 |
PIRi | A25486 A28055 C25486 |
RefSeqi | NP_036873.1, NM_012741.1 [P08934-1] |
3D structure databases
AlphaFoldDBi | P08934 |
SMRi | P08934 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P08934, 1 interactor |
STRINGi | 10116.ENSRNOP00000065146 |
Protein family/group databases
MEROPSi | I25.018 |
PTM databases
GlyGeni | P08934, 5 sites |
PhosphoSitePlusi | P08934 |
Proteomic databases
jPOSTi | P08934 |
PaxDbi | P08934 |
PRIDEi | P08934 |
Genome annotation databases
GeneIDi | 25087 |
KEGGi | rno:25087 |
UCSCi | RGD:2980, rat [P08934-1] |
Organism-specific databases
CTDi | 25087 |
RGDi | 2980, Kng1 |
Phylogenomic databases
eggNOGi | ENOG502RYAC, Eukaryota |
InParanoidi | P08934 |
Miscellaneous databases
PROi | PR:P08934 |
Family and domain databases
CDDi | cd00042, CY, 3 hits |
InterProi | View protein in InterPro IPR000010, Cystatin_dom IPR046350, Cystatin_sf IPR002395, Kininogen IPR027358, Kininogen-type_cystatin_dom IPR018073, Prot_inh_cystat_CS |
Pfami | View protein in Pfam PF00031, Cystatin, 3 hits |
PRINTSi | PR00334, KININOGEN |
SMARTi | View protein in SMART SM00043, CY, 3 hits |
SUPFAMi | SSF54403, SSF54403, 3 hits |
PROSITEi | View protein in PROSITE PS00287, CYSTATIN, 2 hits PS51647, CYSTATIN_KININOGEN, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KNG1_RAT | |
Accessioni | P08934Primary (citable) accession number: P08934 Secondary accession number(s): P08933 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | November 1, 1988 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |