Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-mammal toxin Css2

Gene
N/A
Organism
Centruroides suffusus suffusus (Mexican scorpion)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Beta toxin that binds site-4 of sodium channels (Nav) and reduces peak current (observed on Nav1.6/SCN8A (IC50=307 nM)), shifts the voltage of activation toward more negative potentials (observed on Nav1.6, Nav1.1 (weak), Nav1.2 (weak), and Nav1.7 (weak)), and induces resurgent currents at negative voltages following brief and strong depolarizations (observed on Nav1.6, Nav1.1 (weak), and Nav1.7 (weak)) (PubMed:22200496). A reduction of peak current of Nav1.5/SCN7A has been observed in another study (IC50=35-40 nM) (PubMed:22251893). This toxin is only active on mammals (PubMed:17544584). It has been shown to bind phospholipids (PubMed:15632158).5 Publications

Miscellaneous

Is the most abundant and deadly toxin from the venom of C.s.suffusus.1 Publication
Does not show inhibition of peak current on Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.5 and Nav1.7 (at 280 nM).1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mammal toxin Css2Curated
Alternative name(s):
Css II2 Publications
Short name:
CssII4 Publications
OrganismiCentruroides suffusus suffusus (Mexican scorpion)
Taxonomic identifieri6881 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeCentruroides

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 25 µg/kg by subcutaneous injection into mice and 0.25 µg/kg by intracerebroventricular injection.1 Publication
LD(100) is 3 µg/kg by intracranial injection into mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15E → R: Non-amidated peptide that is not toxic to mice at concentrations up to 30 ug/20 g mouse body weight, when injected intraperitoneally. 1 Publication1
Mutagenesisi64 – 66TCN → RCR: Non-amidated peptide that is as potent as the amidated native peptide. 1 Publication3
Mutagenesisi64 – 66TCN → RCS: Non-amidated peptide that is almost inactive. 1 Publication3
Mutagenesisi66N → H: Non-amidated peptide that is less potent than the native peptide. 1 Publication1
Mutagenesisi66N → R: Non-amidated peptide that is less potent than the native peptide. 1 Publication1
Mutagenesisi66N → S: Non-amidated peptide that is almost inactive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000667771 – 66Beta-mammal toxin Css21 PublicationAdd BLAST66

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi12 ↔ 65PROSITE-ProRule annotation1 PublicationImported
Disulfide bondi16 ↔ 41PROSITE-ProRule annotation1 PublicationImported
Disulfide bondi25 ↔ 46PROSITE-ProRule annotation1 PublicationImported
Disulfide bondi29 ↔ 48PROSITE-ProRule annotation1 PublicationImported
Modified residuei66Asparagine amide2 Publications1

Post-translational modificationi

C-terminal amidation increases its affinity for sodium channels.1 Publication

Keywords - PTMi

Amidation, Disulfide bond

Proteomic databases

PRIDEiP08900

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

Secondary structure

166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08900
SMRiP08900
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 66LCN-type CS-alpha/betaPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10, 1 hit
InterProiView protein in InterPro
IPR003614 Scorpion_toxin-like
IPR036574 Scorpion_toxin-like_sf
IPR018218 Scorpion_toxinL
IPR002061 Scorpion_toxinL/defensin
PfamiView protein in Pfam
PF00537 Toxin_3, 1 hit
PRINTSiPR00285 SCORPNTOXIN
SMARTiView protein in SMART
SM00505 Knot1, 1 hit
SUPFAMiSSF57095 SSF57095, 1 hit
PROSITEiView protein in PROSITE
PS51863 LCN_CSAB, 1 hit

Sequencei

Sequence statusi: Complete.

P08900-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
KEGYLVSKST GCKYECLKLG DNDYCLRECK QQYGKSSGGY CYAFACWCTH
60
LYEQAVVWPL PNKTCN
Length:66
Mass (Da):7,547
Last modified:November 1, 1988 - v1
Checksum:iAEC8FE5FD071418C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27R → G no nucleotide entry (PubMed:17544584).Curated1

Mass spectrometryi

Molecular mass is 7537.0 Da from positions 1 - 66. Determined by ESI. 2 Publications
Molecular mass is 7537.6 Da from positions 1 - 66. Determined by ESI. 1 Publication

Sequence databases

PIRiA27406

Similar proteinsi

Cross-referencesi

Sequence databases

PIRiA27406

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LI7NMR-A1-66[»]
2LJMNMR-A1-66[»]
ProteinModelPortaliP08900
SMRiP08900
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP08900

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.30.10, 1 hit
InterProiView protein in InterPro
IPR003614 Scorpion_toxin-like
IPR036574 Scorpion_toxin-like_sf
IPR018218 Scorpion_toxinL
IPR002061 Scorpion_toxinL/defensin
PfamiView protein in Pfam
PF00537 Toxin_3, 1 hit
PRINTSiPR00285 SCORPNTOXIN
SMARTiView protein in SMART
SM00505 Knot1, 1 hit
SUPFAMiSSF57095 SSF57095, 1 hit
PROSITEiView protein in PROSITE
PS51863 LCN_CSAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSCX2_CENSU
AccessioniPrimary (citable) accession number: P08900
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 18, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again