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Protein

Chymotrypsin-like elastase family member 3B

Gene

CELA3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Efficient protease with alanine specificity but only little elastolytic activity.

Caution

Was originally thought to be elastase 1.1 Publication

Catalytic activityi

Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Charge relay systemBy similarity1
Active sitei123Charge relay systemBy similarity1
Active sitei217Charge relay systemBy similarity1

GO - Molecular functioni

  • peptidase activity Source: ProtInc
  • serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.205

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 3B (EC:3.4.21.70)
Alternative name(s):
Elastase IIIB
Elastase-3B
Protease E
Gene namesi
Name:CELA3B
Synonyms:ELA3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000219073.7
HGNCiHGNC:15945 CELA3B
neXtProtiNX_P08861

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi23436
OpenTargetsiENSG00000219073
PharmGKBiPA27737

Polymorphism and mutation databases

BioMutaiCELA3B
DMDMi317373457

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Or 16Sequence analysisAdd BLAST15
PropeptideiPRO_000002769916 – 28Activation peptideSequence analysisAdd BLAST13
ChainiPRO_000002770029 – 270Chymotrypsin-like elastase family member 3BAdd BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
GlycosylationiCAR_000212114N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi117 ↔ 120Curated
Disulfide bondi157 ↔ 223PROSITE-ProRule annotation
Disulfide bondi188 ↔ 204PROSITE-ProRule annotation
Disulfide bondi213 ↔ 244PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP08861
PeptideAtlasiP08861
PRIDEiP08861
ProteomicsDBi52169

2D gel databases

SWISS-2DPAGEiP08861

PTM databases

GlyConnecti125
iPTMnetiP08861
PhosphoSitePlusiP08861
UniCarbKBiP08861

Expressioni

Tissue specificityi

Pancreas. Not detectable in keratinocytes.1 Publication

Gene expression databases

BgeeiENSG00000219073 Expressed in 101 organ(s), highest expression level in duodenum
CleanExiHS_ELA3B
ExpressionAtlasiP08861 baseline and differential
GenevisibleiP08861 HS

Organism-specific databases

HPAiHPA028086
HPA045650

Interactioni

Protein-protein interaction databases

BioGridi117004, 9 interactors
STRINGi9606.ENSP00000338369

Structurei

3D structure databases

ProteinModelPortaliP08861
SMRiP08861
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 268Peptidase S1PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119027
HOGENOMiHOG000251820
InParanoidiP08861
KOiK01345
OMAiVDHEHCS
OrthoDBiEOG091G0DF7
PhylomeDBiP08861
TreeFamiTF330455

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P08861-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMLRLLSSLL LVAVASGYGP PSSRPSSRVV NGEDAVPYSW PWQVSLQYEK
60 70 80 90 100
SGSFYHTCGG SLIAPDWVVT AGHCISSSRT YQVVLGEYDR AVKEGPEQVI
110 120 130 140 150
PINSGDLFVH PLWNRSCVAC GNDIALIKLS RSAQLGDAVQ LASLPPAGDI
160 170 180 190 200
LPNETPCYIT GWGRLYTNGP LPDKLQEALL PVVDYEHCSR WNWWGSSVKK
210 220 230 240 250
TMVCAGGDIR SGCNGDSGGP LNCPTEDGGW QVHGVTSFVS AFGCNTRRKP
260 270
TVFTRVSAFI DWIEETIASH
Length:270
Mass (Da):29,263
Last modified:January 11, 2011 - v3
Checksum:iF738C5F8F5195D8C
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MRS2A0A0A0MRS2_HUMAN
Chymotrypsin-like elastase family m...
CELA3B
164Annotation score:
A0A0A0MSA6A0A0A0MSA6_HUMAN
Chymotrypsin-like elastase family m...
CELA3B
180Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4R → G in AAA36482 (PubMed:15489334).Curated1
Sequence conflicti64A → G in AAA36482 (PubMed:15489334).Curated1
Sequence conflicti129 – 131Missing AA sequence (PubMed:2737288).Curated3
Sequence conflicti164R → P in AAA36482 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02544679R → W3 PublicationsCorresponds to variant dbSNP:rs7528405Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16630 mRNA Translation: AAA36482.1
AK315798 mRNA Translation: BAG38141.1
AL590556 Genomic DNA No translation available.
CH471134 Genomic DNA Translation: EAW94999.1
BC005216 mRNA Translation: AAH05216.1
M18692 mRNA Translation: AAA58454.1
CCDSiCCDS219.1
PIRiB29934
RefSeqiNP_031378.1, NM_007352.3
UniGeneiHs.728752

Genome annotation databases

EnsembliENST00000337107; ENSP00000338369; ENSG00000219073
GeneIDi23436
KEGGihsa:23436
UCSCiuc001bfk.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16630 mRNA Translation: AAA36482.1
AK315798 mRNA Translation: BAG38141.1
AL590556 Genomic DNA No translation available.
CH471134 Genomic DNA Translation: EAW94999.1
BC005216 mRNA Translation: AAH05216.1
M18692 mRNA Translation: AAA58454.1
CCDSiCCDS219.1
PIRiB29934
RefSeqiNP_031378.1, NM_007352.3
UniGeneiHs.728752

3D structure databases

ProteinModelPortaliP08861
SMRiP08861
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117004, 9 interactors
STRINGi9606.ENSP00000338369

Protein family/group databases

MEROPSiS01.205

PTM databases

GlyConnecti125
iPTMnetiP08861
PhosphoSitePlusiP08861
UniCarbKBiP08861

Polymorphism and mutation databases

BioMutaiCELA3B
DMDMi317373457

2D gel databases

SWISS-2DPAGEiP08861

Proteomic databases

PaxDbiP08861
PeptideAtlasiP08861
PRIDEiP08861
ProteomicsDBi52169

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337107; ENSP00000338369; ENSG00000219073
GeneIDi23436
KEGGihsa:23436
UCSCiuc001bfk.4 human

Organism-specific databases

CTDi23436
DisGeNETi23436
EuPathDBiHostDB:ENSG00000219073.7
GeneCardsiCELA3B
HGNCiHGNC:15945 CELA3B
HPAiHPA028086
HPA045650
neXtProtiNX_P08861
OpenTargetsiENSG00000219073
PharmGKBiPA27737
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119027
HOGENOMiHOG000251820
InParanoidiP08861
KOiK01345
OMAiVDHEHCS
OrthoDBiEOG091G0DF7
PhylomeDBiP08861
TreeFamiTF330455

Miscellaneous databases

GeneWikiiCELA3B
GenomeRNAii23436
PROiPR:P08861

Gene expression databases

BgeeiENSG00000219073 Expressed in 101 organ(s), highest expression level in duodenum
CleanExiHS_ELA3B
ExpressionAtlasiP08861 baseline and differential
GenevisibleiP08861 HS

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCEL3B_HUMAN
AccessioniPrimary (citable) accession number: P08861
Secondary accession number(s): B2RE44
, P11423, Q5VU28, Q5VU29, Q5VU30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 11, 2011
Last modified: September 12, 2018
This is version 172 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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