UniProtKB - P08839 (PT1_ECOLI)
Phosphoenolpyruvate-protein phosphotransferase
ptsI
Functioni
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (PubMed:7876255, PubMed:12705838, PubMed:17053069).
Can also use (Z)-3-fluoro-PEP (ZFPEP), (Z)-3-methyl-PEP (ZMePEP), (Z)-3-chloro-PEP (ZClPEP) and (E)-3-chloro-PEP (EClPEP) as alternative phosphoryl donors (PubMed:12705838).
3 PublicationsMiscellaneous
Catalytic activityi
- L-histidyl-[protein] + phosphoenolpyruvate = Nπ-phospho-L-histidyl-[protein] + pyruvate1 PublicationEC:2.7.3.91 Publication
Cofactori
Activity regulationi
Kineticsi
- KM=0.11 mM for ZFPEP1 Publication
- KM=0.12 mM for EClPEP1 Publication
- KM=0.14 mM for PEP1 Publication
- KM=0.25 mM for ZClPEP1 Publication
- KM=0.43 mM for ZMePEP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 189 | Tele-phosphohistidine intermediate2 Publications | 1 | |
Binding sitei | 296 | PEPBy similarity | 1 | |
Binding sitei | 332 | PEP1 Publication | 1 | |
Metal bindingi | 431 | Magnesium1 Publication | 1 | |
Metal bindingi | 455 | Magnesium1 Publication | 1 | |
Binding sitei | 465 | PEPBy similarity | 1 | |
Active sitei | 502 | Proton donor1 Publication1 Publication | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- kinase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- phosphoenolpyruvate-protein phosphotransferase activity Source: UniProtKB-EC
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PTSI-MONOMER |
BRENDAi | 2.7.3.9, 2026 |
SABIO-RKi | P08839 |
Protein family/group databases
TCDBi | 8.A.7.1.1, the phosphotransferase system enzyme i (ei) family |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoenolpyruvate-protein phosphotransferase1 Publication (EC:2.7.3.91 Publication)Alternative name(s): Phosphotransferase system, enzyme I1 Publication |
Gene namesi | Name:ptsI1 Publication Ordered Locus Names:b2416, JW2409 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 189 | H → A: Very strong decrease of the affinity and catalytic efficiency for PEP. Inactive; when associated with A-502. 1 Publication | 1 | |
Mutagenesisi | 502 | C → A: Inactive; when associated with A-189. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000147067 | 1 – 575 | Phosphoenolpyruvate-protein phosphotransferaseAdd BLAST | 575 |
Proteomic databases
jPOSTi | P08839 |
PaxDbi | P08839 |
PRIDEi | P08839 |
2D gel databases
SWISS-2DPAGEi | P08839 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsBinary interactionsi
P08839
With | #Exp. | IntAct |
---|---|---|
bglG [P11989] | 3 | EBI-551533,EBI-545674 |
ptsH [P0AA04] | 4 | EBI-551533,EBI-902853 |
itself | 2 | EBI-551533,EBI-551533 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260570, 520 interactors 851219, 3 interactors |
DIPi | DIP-10603N |
IntActi | P08839, 14 interactors |
MINTi | P08839 |
STRINGi | 511145.b2416 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P08839 |
BMRBi | P08839 |
SMRi | P08839 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08839 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 454 – 455 | PEP binding1 Publication | 2 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1080, Bacteria |
HOGENOMi | CLU_007308_7_0_6 |
InParanoidi | P08839 |
OMAi | CNAEWAL |
PhylomeDBi | P08839 |
Family and domain databases
Gene3Di | 1.10.274.10, 1 hit 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR008279, PEP-util_enz_mobile_dom IPR018274, PEP_util_AS IPR000121, PEP_util_C IPR023151, PEP_util_CS IPR036637, Phosphohistidine_dom_sf IPR024692, PTS_EI IPR006318, PTS_EI-like IPR008731, PTS_EIN IPR036618, PtsI_HPr-bd_sf IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
Pfami | View protein in Pfam PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF02896, PEP-utilizers_C, 1 hit |
PIRSFi | PIRSF000732, PTS_enzyme_I, 1 hit |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF51621, SSF51621, 1 hit SSF52009, SSF52009, 1 hit |
TIGRFAMsi | TIGR01417, PTS_I_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00742, PEP_ENZYMES_2, 1 hit PS00370, PEP_ENZYMES_PHOS_SITE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA
60 70 80 90 100
SAQLETIKTK AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA
110 120 130 140 150
DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN ILGLKIIDLS
160 170 180 190 200
AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP
210 220 230 240 250
AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK
260 270 280 290 300
AELAKLKDLP AITLDGHQVE VCANIGTVRD VEGAERNGAE GVGLYRTEFL
310 320 330 340 350
FMDRDALPTE EEQFAAYKAV AEACGSQAVI VRTMDIGGDK ELPYMNFPKE
360 370 380 390 400
ENPFLGWRAI RIAMDRREIL RDQLRAILRA SAFGKLRIMF PMIISVEEVR
410 420 430 440 450
ALRKEIEIYK QELRDEGKAF DESIEIGVMV ETPAAATIAR HLAKEVDFFS
460 470 480 490 500
IGTNDLTQYT LAVDRGNDMI SHLYQPMSPS VLNLIKQVID ASHAEGKWTG
510 520 530 540 550
MCGELAGDER ATLLLLGMGL DEFSMSAISI PRIKKIIRNT NFEDAKVLAE
560 570
QALAQPTTDE LMTLVNKFIE EKTIC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02796 Genomic DNA Translation: AAA24441.1 M10425 Genomic DNA Translation: AAA24439.1 U00096 Genomic DNA Translation: AAC75469.1 AP009048 Genomic DNA Translation: BAA16290.1 M21994 Genomic DNA Translation: AAA24385.1 M21451 Genomic DNA Translation: AAA23656.1 |
PIRi | B29785, WQECPI |
RefSeqi | NP_416911.1, NC_000913.3 WP_000623140.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75469; AAC75469; b2416 BAA16290; BAA16290; BAA16290 |
GeneIDi | 946879 |
KEGGi | ecj:JW2409 eco:b2416 |
PATRICi | fig|1411691.4.peg.4315 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02796 Genomic DNA Translation: AAA24441.1 M10425 Genomic DNA Translation: AAA24439.1 U00096 Genomic DNA Translation: AAC75469.1 AP009048 Genomic DNA Translation: BAA16290.1 M21994 Genomic DNA Translation: AAA24385.1 M21451 Genomic DNA Translation: AAA23656.1 |
PIRi | B29785, WQECPI |
RefSeqi | NP_416911.1, NC_000913.3 WP_000623140.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EZA | NMR | - | A | 1-258 | [»] | |
1EZB | NMR | - | A | 1-258 | [»] | |
1EZC | NMR | - | A | 1-258 | [»] | |
1EZD | NMR | - | A | 1-258 | [»] | |
1ZYM | X-ray | 2.50 | A/B | 1-258 | [»] | |
2EZA | NMR | - | A | 1-258 | [»] | |
2EZB | NMR | - | A | 1-258 | [»] | |
2EZC | NMR | - | A | 1-258 | [»] | |
2HWG | X-ray | 2.70 | A/B | 1-575 | [»] | |
2KX9 | Other | - | A/B | 1-573 | [»] | |
2L5H | Other | - | A/B | 1-573 | [»] | |
2MP0 | NMR | - | A | 1-258 | [»] | |
2N5T | Other | - | A/B | 1-575 | [»] | |
2XDF | Other | - | A/B | 1-573 | [»] | |
3EZA | NMR | - | A | 1-249 | [»] | |
3EZB | NMR | - | A | 1-258 | [»] | |
3EZE | NMR | - | A | 1-258 | [»] | |
6V9K | X-ray | 1.90 | A/B | 261-575 | [»] | |
6VU0 | X-ray | 3.50 | A/B | 261-575 | [»] | |
AlphaFoldDBi | P08839 | |||||
BMRBi | P08839 | |||||
SMRi | P08839 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260570, 520 interactors 851219, 3 interactors |
DIPi | DIP-10603N |
IntActi | P08839, 14 interactors |
MINTi | P08839 |
STRINGi | 511145.b2416 |
Protein family/group databases
TCDBi | 8.A.7.1.1, the phosphotransferase system enzyme i (ei) family |
2D gel databases
SWISS-2DPAGEi | P08839 |
Proteomic databases
jPOSTi | P08839 |
PaxDbi | P08839 |
PRIDEi | P08839 |
Genome annotation databases
EnsemblBacteriai | AAC75469; AAC75469; b2416 BAA16290; BAA16290; BAA16290 |
GeneIDi | 946879 |
KEGGi | ecj:JW2409 eco:b2416 |
PATRICi | fig|1411691.4.peg.4315 |
Organism-specific databases
EchoBASEi | EB0782 |
Phylogenomic databases
eggNOGi | COG1080, Bacteria |
HOGENOMi | CLU_007308_7_0_6 |
InParanoidi | P08839 |
OMAi | CNAEWAL |
PhylomeDBi | P08839 |
Enzyme and pathway databases
BioCyci | EcoCyc:PTSI-MONOMER |
BRENDAi | 2.7.3.9, 2026 |
SABIO-RKi | P08839 |
Miscellaneous databases
EvolutionaryTracei | P08839 |
PROi | PR:P08839 |
Family and domain databases
Gene3Di | 1.10.274.10, 1 hit 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR008279, PEP-util_enz_mobile_dom IPR018274, PEP_util_AS IPR000121, PEP_util_C IPR023151, PEP_util_CS IPR036637, Phosphohistidine_dom_sf IPR024692, PTS_EI IPR006318, PTS_EI-like IPR008731, PTS_EIN IPR036618, PtsI_HPr-bd_sf IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
Pfami | View protein in Pfam PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF02896, PEP-utilizers_C, 1 hit |
PIRSFi | PIRSF000732, PTS_enzyme_I, 1 hit |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF51621, SSF51621, 1 hit SSF52009, SSF52009, 1 hit |
TIGRFAMsi | TIGR01417, PTS_I_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00742, PEP_ENZYMES_2, 1 hit PS00370, PEP_ENZYMES_PHOS_SITE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PT1_ECOLI | |
Accessioni | P08839Primary (citable) accession number: P08839 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | November 1, 1988 | |
Last modified: | May 25, 2022 | |
This is version 207 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families