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Protein

Guanine nucleotide-binding protein G(i) subunit alpha-2

Gene

Gnai2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47MagnesiumBy similarity1
Metal bindingi182MagnesiumBy similarity1
Binding sitei327GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47GTPBy similarity8
Nucleotide bindingi176 – 182GTPBy similarity7
Nucleotide bindingi201 – 205GTPBy similarity5
Nucleotide bindingi270 – 273GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer
Biological processCell cycle, Cell division
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-170670 Adenylate cyclase inhibitory pathway
R-MMU-202040 G-protein activation
R-MMU-392170 ADP signalling through P2Y purinoceptor 12
R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(i) subunit alpha-2
Alternative name(s):
Adenylate cyclase-inhibiting G alpha protein
Gene namesi
Name:Gnai2
Synonyms:Gnai-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95772 Gnai2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002036812 – 355Guanine nucleotide-binding protein G(i) subunit alpha-2Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi3S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

EPDiP08752
MaxQBiP08752
PaxDbiP08752
PeptideAtlasiP08752
PRIDEiP08752

PTM databases

iPTMnetiP08752
PhosphoSitePlusiP08752
SwissPalmiP08752

Expressioni

Gene expression databases

BgeeiENSMUSG00000032562 Expressed in 341 organ(s), highest expression level in spleen
ExpressionAtlasiP08752 baseline and differential
GenevisibleiP08752 MM

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UNC5B. Interacts with GPSM1. Interacts with RGS12 and RGS14 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199967, 6 interactors
CORUMiP08752
DIPiDIP-605N
IntActiP08752, 8 interactors
MINTiP08752
STRINGi10090.ENSMUSP00000057543

Structurei

3D structure databases

ProteinModelPortaliP08752
SMRiP08752
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038730
HOVERGENiHBG063184
InParanoidiP08752
KOiK04630
OMAiEQGIMPD
OrthoDBiEOG091G0VUT
TreeFamiTF300673

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P08752-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPQR
110 120 130 140 150
ADDARQLFAL SCAAEEQGML PEDLSGVIRR LWADHGVQAC FGRSREYQLN
160 170 180 190 200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF
210 220 230 240 250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT QSSLTICFPE YTGANKYDEA
310 320 330 340 350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK

DCGLF
Length:355
Mass (Da):40,489
Last modified:July 27, 2011 - v5
Checksum:i90AC64AFA713493E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YWA9A0A0A6YWA9_MOUSE
Guanine nucleotide-binding protein ...
Gnai2
160Annotation score:
A0A0A6YXN0A0A0A6YXN0_MOUSE
Guanine nucleotide-binding protein ...
Gnai2
39Annotation score:
A0A0A6YXC2A0A0A6YXC2_MOUSE
Guanine nucleotide-binding protein ...
Gnai2
85Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82M → I in AAB30632 (PubMed:8170357).Curated1
Sequence conflicti82M → L in AAA37692 (PubMed:3092218).Curated1
Sequence conflicti87A → R in AAA37692 (PubMed:3092218).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13963 mRNA Translation: AAA37692.1
AK157998 mRNA Translation: BAE34308.1
AK159222 mRNA Translation: BAE34909.1
AK167388 mRNA Translation: BAE39478.1
BC065159 mRNA Translation: AAH65159.1
S71213 mRNA Translation: AAB30632.2
CCDSiCCDS23502.1
PIRiB25889 RGMSI2
RefSeqiNP_032164.2, NM_008138.4
XP_006511700.1, XM_006511637.2
UniGeneiMm.196464

Genome annotation databases

EnsembliENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562
ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562
GeneIDi14678
KEGGimmu:14678
UCSCiuc009rml.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13963 mRNA Translation: AAA37692.1
AK157998 mRNA Translation: BAE34308.1
AK159222 mRNA Translation: BAE34909.1
AK167388 mRNA Translation: BAE39478.1
BC065159 mRNA Translation: AAH65159.1
S71213 mRNA Translation: AAB30632.2
CCDSiCCDS23502.1
PIRiB25889 RGMSI2
RefSeqiNP_032164.2, NM_008138.4
XP_006511700.1, XM_006511637.2
UniGeneiMm.196464

3D structure databases

ProteinModelPortaliP08752
SMRiP08752
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199967, 6 interactors
CORUMiP08752
DIPiDIP-605N
IntActiP08752, 8 interactors
MINTiP08752
STRINGi10090.ENSMUSP00000057543

PTM databases

iPTMnetiP08752
PhosphoSitePlusiP08752
SwissPalmiP08752

Proteomic databases

EPDiP08752
MaxQBiP08752
PaxDbiP08752
PeptideAtlasiP08752
PRIDEiP08752

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562
ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562
GeneIDi14678
KEGGimmu:14678
UCSCiuc009rml.1 mouse

Organism-specific databases

CTDi2771
MGIiMGI:95772 Gnai2

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038730
HOVERGENiHBG063184
InParanoidiP08752
KOiK04630
OMAiEQGIMPD
OrthoDBiEOG091G0VUT
TreeFamiTF300673

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-170670 Adenylate cyclase inhibitory pathway
R-MMU-202040 G-protein activation
R-MMU-392170 ADP signalling through P2Y purinoceptor 12
R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Miscellaneous databases

ChiTaRSiGnai2 mouse
PROiPR:P08752
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032562 Expressed in 341 organ(s), highest expression level in spleen
ExpressionAtlasiP08752 baseline and differential
GenevisibleiP08752 MM

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGNAI2_MOUSE
AccessioniPrimary (citable) accession number: P08752
Secondary accession number(s): Q3TXK7, Q6P1C0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 179 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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