UniProtKB - P08752 (GNAI2_MOUSE)
Guanine nucleotide-binding protein G(i) subunit alpha-2
Gnai2
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | MagnesiumBy similarity | 1 | |
Metal bindingi | 182 | MagnesiumBy similarity | 1 | |
Binding sitei | 327 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 40 – 47 | GTPBy similarity | 8 | |
Nucleotide bindingi | 176 – 182 | GTPBy similarity | 7 | |
Nucleotide bindingi | 201 – 205 | GTPBy similarity | 5 | |
Nucleotide bindingi | 270 – 273 | GTPBy similarity | 4 |
GO - Molecular functioni
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
- GTPase activity Source: GO_Central
- GTP binding Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: MGI
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: MGI
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB
- cell population proliferation Source: MGI
- gamma-aminobutyric acid signaling pathway Source: MGI
- G protein-coupled acetylcholine receptor signaling pathway Source: MGI
- G protein-coupled adenosine receptor signaling pathway Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process Source: MGI
- negative regulation of apoptotic signaling pathway Source: MGI
- negative regulation of calcium ion-dependent exocytosis Source: MGI
- negative regulation of protein tyrosine phosphatase activity Source: MGI
- negative regulation of synaptic transmission Source: MGI
- positive regulation of cell migration Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of insulin receptor signaling pathway Source: MGI
- positive regulation of NAD(P)H oxidase activity Source: MGI
- positive regulation of neural precursor cell proliferation Source: MGI
- positive regulation of renal sodium excretion Source: MGI
- positive regulation of superoxide anion generation Source: MGI
- positive regulation of urine volume Source: MGI
- positive regulation of vascular associated smooth muscle cell proliferation Source: MGI
- regulation of calcium ion transport Source: MGI
Keywordsi
Molecular function | Transducer |
Biological process | Cell cycle, Cell division |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-112043, PLC beta mediated events R-MMU-170670, Adenylate cyclase inhibitory pathway R-MMU-202040, G-protein activation R-MMU-392170, ADP signalling through P2Y purinoceptor 12 R-MMU-400042, Adrenaline,noradrenaline inhibits insulin secretion R-MMU-418594, G alpha (i) signalling events R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-MMU-9009391, Extra-nuclear estrogen signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(i) subunit alpha-2Alternative name(s): Adenylate cyclase-inhibiting G alpha protein |
Gene namesi | Name:Gnai2 Synonyms:Gnai-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95772, Gnai2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytoskeleton
- centrosome By similarity
Other locations
- Cytoplasm By similarity
- Membrane By similarity; Lipid-anchor By similarity
Note: Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody (By similarity).By similarity
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000203681 | 2 – 355 | Guanine nucleotide-binding protein G(i) subunit alpha-2Add BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Lipidationi | 3 | S-palmitoyl cysteineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
EPDi | P08752 |
jPOSTi | P08752 |
MaxQBi | P08752 |
PaxDbi | P08752 |
PeptideAtlasi | P08752 |
PRIDEi | P08752 |
PTM databases
iPTMneti | P08752 |
PhosphoSitePlusi | P08752 |
SwissPalmi | P08752 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032562, Expressed in spleen and 354 other tissues |
ExpressionAtlasi | P08752, baseline and differential |
Genevisiblei | P08752, MM |
Interactioni
Subunit structurei
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Interacts with UNC5B (By similarity).
Interacts with GPSM1 (By similarity).
Interacts with RGS12 and RGS14 (By similarity).
Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI3 (By similarity).
Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif) (By similarity).
Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif) (By similarity).
By similarityGO - Molecular functioni
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 199967, 38 interactors |
CORUMi | P08752 |
DIPi | DIP-605N |
IntActi | P08752, 8 interactors |
MINTi | P08752 |
STRINGi | 10090.ENSMUSP00000057543 |
Miscellaneous databases
RNActi | P08752, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 355 | G-alphaPROSITE-ProRule annotationAdd BLAST | 324 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 48 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 174 – 182 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 197 – 206 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 266 – 273 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 325 – 330 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000155125 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P08752 |
OMAi | PNKYEEA |
OrthoDBi | 754573at2759 |
TreeFami | TF300673 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPQR
110 120 130 140 150
ADDARQLFAL SCAAEEQGML PEDLSGVIRR LWADHGVQAC FGRSREYQLN
160 170 180 190 200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF
210 220 230 240 250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT QSSLTICFPE YTGANKYDEA
310 320 330 340 350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK
DCGLF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YWA9 | A0A0A6YWA9_MOUSE | Guanine nucleotide-binding protein ... | Gnai2 | 160 | Annotation score: | ||
A0A0A6YXN0 | A0A0A6YXN0_MOUSE | Guanine nucleotide-binding protein ... | Gnai2 | 39 | Annotation score: | ||
A0A0A6YXC2 | A0A0A6YXC2_MOUSE | Guanine nucleotide-binding protein ... | Gnai2 | 85 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 82 | M → I in AAB30632 (PubMed:8170357).Curated | 1 | |
Sequence conflicti | 82 | M → L in AAA37692 (PubMed:3092218).Curated | 1 | |
Sequence conflicti | 87 | A → R in AAA37692 (PubMed:3092218).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13963 mRNA Translation: AAA37692.1 AK157998 mRNA Translation: BAE34308.1 AK159222 mRNA Translation: BAE34909.1 AK167388 mRNA Translation: BAE39478.1 BC065159 mRNA Translation: AAH65159.1 S71213 mRNA Translation: AAB30632.2 |
CCDSi | CCDS23502.1 |
PIRi | B25889, RGMSI2 |
RefSeqi | NP_032164.2, NM_008138.4 XP_006511700.1, XM_006511637.2 |
Genome annotation databases
Ensembli | ENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562 ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562 |
GeneIDi | 14678 |
KEGGi | mmu:14678 |
UCSCi | uc009rml.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13963 mRNA Translation: AAA37692.1 AK157998 mRNA Translation: BAE34308.1 AK159222 mRNA Translation: BAE34909.1 AK167388 mRNA Translation: BAE39478.1 BC065159 mRNA Translation: AAH65159.1 S71213 mRNA Translation: AAB30632.2 |
CCDSi | CCDS23502.1 |
PIRi | B25889, RGMSI2 |
RefSeqi | NP_032164.2, NM_008138.4 XP_006511700.1, XM_006511637.2 |
3D structure databases
SMRi | P08752 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199967, 38 interactors |
CORUMi | P08752 |
DIPi | DIP-605N |
IntActi | P08752, 8 interactors |
MINTi | P08752 |
STRINGi | 10090.ENSMUSP00000057543 |
PTM databases
iPTMneti | P08752 |
PhosphoSitePlusi | P08752 |
SwissPalmi | P08752 |
Proteomic databases
EPDi | P08752 |
jPOSTi | P08752 |
MaxQBi | P08752 |
PaxDbi | P08752 |
PeptideAtlasi | P08752 |
PRIDEi | P08752 |
Protocols and materials databases
Antibodypediai | 2180, 284 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562 ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562 |
GeneIDi | 14678 |
KEGGi | mmu:14678 |
UCSCi | uc009rml.1, mouse |
Organism-specific databases
CTDi | 2771 |
MGIi | MGI:95772, Gnai2 |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000155125 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P08752 |
OMAi | PNKYEEA |
OrthoDBi | 754573at2759 |
TreeFami | TF300673 |
Enzyme and pathway databases
Reactomei | R-MMU-112043, PLC beta mediated events R-MMU-170670, Adenylate cyclase inhibitory pathway R-MMU-202040, G-protein activation R-MMU-392170, ADP signalling through P2Y purinoceptor 12 R-MMU-400042, Adrenaline,noradrenaline inhibits insulin secretion R-MMU-418594, G alpha (i) signalling events R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-MMU-9009391, Extra-nuclear estrogen signaling |
Miscellaneous databases
BioGRID-ORCSi | 14678, 3 hits in 20 CRISPR screens |
ChiTaRSi | Gnai2, mouse |
PROi | PR:P08752 |
RNActi | P08752, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032562, Expressed in spleen and 354 other tissues |
ExpressionAtlasi | P08752, baseline and differential |
Genevisiblei | P08752, MM |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAI2_MOUSE | |
Accessioni | P08752Primary (citable) accession number: P08752 Secondary accession number(s): Q3TXK7, Q6P1C0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 197 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families