Gnai2

Functionⁱ

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	47	MagnesiumBy similarity	1
Metal bindingⁱ	182	MagnesiumBy similarity	1
Binding siteⁱ	327	GTP; via amide nitrogenBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	40 – 47	GTPBy similarity	8
Nucleotide bindingⁱ	176 – 182	GTPBy similarity	7
Nucleotide bindingⁱ	201 – 205	GTPBy similarity	5
Nucleotide bindingⁱ	270 – 273	GTPBy similarity	4

GO - Molecular functionⁱ

G-protein beta/gamma-subunit complex binding Source: GO_Central
G-protein coupled receptor binding Source: GO_Central
GTPase activity Source: GO_Central
GTP binding
Source: MGI
Inferred from sequence alignmentⁱ
- 3092218
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenosine receptor signaling pathway Source: MGI
adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
Source: MGI
Inferred from mutant phenotypeⁱ
- 8622915
cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB
cell proliferation
Source: MGI
Inferred from physical interactionⁱ
- 14712229
gamma-aminobutyric acid signaling pathway Source: MGI
G-protein coupled acetylcholine receptor signaling pathway
Source: MGI
Inferred from mutant phenotypeⁱ
- 11299198
G-protein coupled receptor signaling pathway Source: MGI
negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process Source: MGI
negative regulation of apoptotic signaling pathway Source: MGI
negative regulation of calcium ion-dependent exocytosis Source: MGI
negative regulation of protein tyrosine phosphatase activity Source: MGI
negative regulation of synaptic transmission Source: MGI
positive regulation of cell migration Source: MGI
positive regulation of cell proliferation Source: MGI
positive regulation of ERK1 and ERK2 cascade Source: MGI
positive regulation of insulin receptor signaling pathway Source: MGI
positive regulation of NAD(P)H oxidase activity Source: MGI
positive regulation of neural precursor cell proliferation Source: MGI
positive regulation of renal sodium excretion Source: MGI
positive regulation of superoxide anion generation Source: MGI
positive regulation of urine volume Source: MGI
positive regulation of vascular smooth muscle cell proliferation Source: MGI
regulation of calcium ion transport Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transducer
Biological process	Cell cycle, Cell division
Ligand	GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-112043 PLC beta mediated events R-MMU-170670 Adenylate cyclase inhibitory pathway R-MMU-202040 G-protein activation R-MMU-392170 ADP signalling through P2Y purinoceptor 12 R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion R-MMU-418594 G alpha (i) signalling events R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Reactomeⁱ

R-MMU-112043 PLC beta mediated events
R-MMU-170670 Adenylate cyclase inhibitory pathway
R-MMU-202040 G-protein activation
R-MMU-392170 ADP signalling through P2Y purinoceptor 12
R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Guanine nucleotide-binding protein G(i) subunit alpha-2 Alternative name(s): Adenylate cyclase-inhibiting G alpha protein
Gene namesⁱ	Name:Gnai2 Synonyms:Gnai-2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 9

Organism-specific databases

MGIⁱ	MGI:95772 Gnai2

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000203681	2 – 355	Guanine nucleotide-binding protein G(i) subunit alpha-2Add BLAST		354

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Lipidationⁱ	2	N-myristoyl glycineBy similarity		1
Lipidationⁱ	3	S-palmitoyl cysteineBy similarity		1

Keywords - PTMⁱ

Lipoprotein, Myristate, Palmitate

Proteomic databases

EPDⁱ	P08752
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P08752
PaxDbⁱ	P08752
PeptideAtlas More...PeptideAtlasⁱ	P08752
PRIDEⁱ	P08752

PTM databases

iPTMnetⁱ	P08752
PhosphoSitePlusⁱ	P08752
SwissPalmⁱ	P08752

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000032562 Expressed in 341 organ(s), highest expression level in spleen
ExpressionAtlasⁱ	P08752 baseline and differential
Genevisibleⁱ	P08752 MM

Interactionⁱ

Subunit structureⁱ

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UNC5B. Interacts with GPSM1. Interacts with RGS12 and RGS14 (By similarity).By similarity

GO - Molecular functionⁱ

G-protein coupled receptor binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	199967, 6 interactors
CORUMⁱ	P08752
Database of interacting proteins More...DIPⁱ	DIP-605N
IntActⁱ	P08752, 8 interactors
Molecular INTeraction database More...MINTⁱ	P08752
STRINGⁱ	10090.ENSMUSP00000057543

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P08752
SMRⁱ	P08752
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0082 Eukaryota ENOG410XNVQ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118851
HOGENOMⁱ	HOG000038730
HOVERGENⁱ	HBG063184
InParanoidⁱ	P08752
KEGG Orthology (KO) More...KOⁱ	K04630
OMAⁱ	EQGIMPD
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0VUT
TreeFamⁱ	TF300673

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00066 G-alpha, 1 hit
Gene3Dⁱ	1.10.400.10, 1 hit
InterProⁱ	View protein in InterPro IPR001408 Gprotein_alpha_I IPR001019 Gprotein_alpha_su IPR011025 GproteinA_insert IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR10218 PTHR10218, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00503 G-alpha, 1 hit
PRINTSⁱ	PR00318 GPROTEINA PR00441 GPROTEINAI
SMARTⁱ	View protein in SMART SM00275 G_alpha, 1 hit
SUPFAMⁱ	SSF47895 SSF47895, 1 hit SSF52540 SSF52540, 2 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show all Align All

P08752-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV 
        60         70         80         90        100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPQR 
       110        120        130        140        150
ADDARQLFAL SCAAEEQGML PEDLSGVIRR LWADHGVQAC FGRSREYQLN 
       160        170        180        190        200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF 
       210        220        230        240        250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL 
       260        270        280        290        300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT QSSLTICFPE YTGANKYDEA 
       310        320        330        340        350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK 

DCGLF

Length:355

Mass (Da):40,489

Last modified:July 27, 2011 - v5

Checksum:ⁱ90AC64AFA713493E

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A0A6YWA9	A0A0A6YWA9_MOUSE	Guanine nucleotide-binding protein ... Guanine nucleotide-binding protein G(i) subunit alpha-2	Gnai2	160	Annotation score:
A0A0A6YXN0	A0A0A6YXN0_MOUSE	Guanine nucleotide-binding protein ... Guanine nucleotide-binding protein G(i) subunit alpha-2	Gnai2	39	Annotation score:
A0A0A6YXC2	A0A0A6YXC2_MOUSE	Guanine nucleotide-binding protein ... Guanine nucleotide-binding protein G(i) subunit alpha-2	Gnai2	85	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	82	M → I in AAB30632 (PubMed:8170357).Curated	1
Sequence conflictⁱ	82	M → L in AAA37692 (PubMed:3092218).Curated	1
Sequence conflictⁱ	87	A → R in AAA37692 (PubMed:3092218).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M13963 mRNA Translation: AAA37692.1 AK157998 mRNA Translation: BAE34308.1 AK159222 mRNA Translation: BAE34909.1 AK167388 mRNA Translation: BAE39478.1 BC065159 mRNA Translation: AAH65159.1 S71213 mRNA Translation: AAB30632.2
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS23502.1
PIRⁱ	B25889 RGMSI2
NCBI Reference Sequences More...RefSeqⁱ	NP_032164.2, NM_008138.4 XP_006511700.1, XM_006511637.2
UniGeneⁱ	Mm.196464

Genome annotation databases

Ensemblⁱ	ENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562 ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562
GeneIDⁱ	14678
KEGGⁱ	mmu:14678
UCSC genome browser More...UCSCⁱ	uc009rml.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P08752	Guanine nucleotide-binding protein G(i) subunit alpha-2 (Fragment)		MOUSE		160	UniRef100_P08752

Protein	Similar proteins		Species	Score	Length	Source
P08752	Guanine nucleotide-binding protein G(i) subunit alpha-2		RAT		355	UniRef90_P04897
Guanine nucleotide-binding protein G(i) subunit alpha-2		CANLF		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CAVPO		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CHICK		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		MACFA		355
+205

Protein	Similar proteins		Species	Score	Length	Source
P08752	Guanine nucleotide-binding protein G(i) subunit alpha-2		RAT		355	UniRef50_P04897
Guanine nucleotide-binding protein G(i) subunit alpha-2		MACFA		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CANLF		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CAVPO		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CHICK		355
+259

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M13963 mRNA Translation: AAA37692.1 AK157998 mRNA Translation: BAE34308.1 AK159222 mRNA Translation: BAE34909.1 AK167388 mRNA Translation: BAE39478.1 BC065159 mRNA Translation: AAH65159.1 S71213 mRNA Translation: AAB30632.2
CCDSⁱ	CCDS23502.1
PIRⁱ	B25889 RGMSI2
RefSeqⁱ	NP_032164.2, NM_008138.4 XP_006511700.1, XM_006511637.2
UniGeneⁱ	Mm.196464

3D structure databases

ProteinModelPortalⁱ	P08752
SMRⁱ	P08752
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199967, 6 interactors
CORUMⁱ	P08752
DIPⁱ	DIP-605N
IntActⁱ	P08752, 8 interactors
MINTⁱ	P08752
STRINGⁱ	10090.ENSMUSP00000057543

PTM databases

iPTMnetⁱ	P08752
PhosphoSitePlusⁱ	P08752
SwissPalmⁱ	P08752

Proteomic databases

EPDⁱ	P08752
MaxQBⁱ	P08752
PaxDbⁱ	P08752
PeptideAtlasⁱ	P08752
PRIDEⁱ	P08752

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562 ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562
GeneIDⁱ	14678
KEGGⁱ	mmu:14678
UCSCⁱ	uc009rml.1 mouse

Organism-specific databases

CTDⁱ	2771
MGIⁱ	MGI:95772 Gnai2

Phylogenomic databases

eggNOGⁱ	KOG0082 Eukaryota ENOG410XNVQ LUCA
GeneTreeⁱ	ENSGT00760000118851
HOGENOMⁱ	HOG000038730
HOVERGENⁱ	HBG063184
InParanoidⁱ	P08752
KOⁱ	K04630
OMAⁱ	EQGIMPD
OrthoDBⁱ	EOG091G0VUT
TreeFamⁱ	TF300673

Enzyme and pathway databases

Reactomeⁱ	R-MMU-112043 PLC beta mediated events R-MMU-170670 Adenylate cyclase inhibitory pathway R-MMU-202040 G-protein activation R-MMU-392170 ADP signalling through P2Y purinoceptor 12 R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion R-MMU-418594 G alpha (i) signalling events R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Reactomeⁱ

R-MMU-112043 PLC beta mediated events
R-MMU-170670 Adenylate cyclase inhibitory pathway
R-MMU-202040 G-protein activation
R-MMU-392170 ADP signalling through P2Y purinoceptor 12
R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Miscellaneous databases

ChiTaRSⁱ	Gnai2 mouse
Protein Ontology More...PROⁱ	PR:P08752
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000032562 Expressed in 341 organ(s), highest expression level in spleen
ExpressionAtlasⁱ	P08752 baseline and differential
Genevisibleⁱ	P08752 MM

Family and domain databases

CDDⁱ	cd00066 G-alpha, 1 hit
Gene3Dⁱ	1.10.400.10, 1 hit
InterProⁱ	View protein in InterPro IPR001408 Gprotein_alpha_I IPR001019 Gprotein_alpha_su IPR011025 GproteinA_insert IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR10218 PTHR10218, 1 hit
Pfamⁱ	View protein in Pfam PF00503 G-alpha, 1 hit
PRINTSⁱ	PR00318 GPROTEINA PR00441 GPROTEINAI
SMARTⁱ	View protein in SMART SM00275 G_alpha, 1 hit
SUPFAMⁱ	SSF47895 SSF47895, 1 hit SSF52540 SSF52540, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GNAI2_MOUSE
Accessionⁱ	P08752Primary (citable) accession number: P08752 Secondary accession number(s): Q3TXK7, Q6P1C0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
	Last sequence update:	July 27, 2011
	Last modified:	September 12, 2018
	This is version 179 of the entry and version 5 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - P08752 (GNAI2_MOUSE)

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Guanine nucleotide-binding protein G(i) subunit alpha-2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Plasma membrane

Other locations

Cytoskeleton

Cytosol

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ