UniProtKB - P08660 (AK3_ECOLI)
Protein
Lysine-sensitive aspartokinase 3
Gene
lysC
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
Aspartokinases I and II also catalyze the same reaction(s).
Catalytic activityi
- EC:2.7.2.4
Activity regulationi
Synthesis and activity are sensitive to the allosteric inhibitor lysine, one of the end metabolites of the aspartic acid family branched pathway.1 Publication
: L-lysine biosynthesis via DAP pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Aspartokinase (lysC), Lysine-sensitive aspartokinase 3 (lysC), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (FAZ83_20520), Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA), Bifunctional aspartokinase/homoserine dehydrogenase 2 (metL)
- Aspartate-semialdehyde dehydrogenase (asd), Aspartate-semialdehyde dehydrogenase (asd)
- 4-hydroxy-tetrahydrodipicolinate synthase (dapA), 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
- 4-hydroxy-tetrahydrodipicolinate reductase (dapB), 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 45 | Substrate | 1 | |
Binding sitei | 119 | Substrate | 1 | |
Binding sitei | 227 | ATP; via amide nitrogen and carbonyl oxygen | 1 | |
Binding sitei | 232 | ATP | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 8 – 11 | ATP | 4 | |
Nucleotide bindingi | 221 – 222 | ATP | 2 | |
Nucleotide bindingi | 257 – 258 | ATP | 2 |
GO - Molecular functioni
- aspartate kinase activity Source: EcoCyc
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- homoserine biosynthetic process Source: EcoCyc
- lysine biosynthetic process via diaminopimelate Source: EcoCyc
Keywordsi
Molecular function | Allosteric enzyme, Kinase, Transferase |
Biological process | Amino-acid biosynthesis, Lysine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ASPKINIII-MONOMER MetaCyc:ASPKINIII-MONOMER |
SABIO-RKi | P08660 |
UniPathwayi | UPA00034;UER00015 |
Names & Taxonomyi
Protein namesi | Recommended name: Lysine-sensitive aspartokinase 3 (EC:2.7.2.4)Alternative name(s): Aspartate kinase III Short name: AKIII Lysine-sensitive aspartokinase III |
Gene namesi | Name:lysC Synonyms:apk Ordered Locus Names:b4024, JW3984 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 8 | K → R: Reduces activity about 98%. Increases KM for aspartate about 40-fold, enzyme is less sensitive to lysine inhibition. 1 Publication | 1 | |
Mutagenesisi | 119 | E → D: Increases KM for aspartate about 3000-fold. 1 Publication | 1 | |
Mutagenesisi | 198 | R → K: Increases KM for aspartate about 200-fold. 1 Publication | 1 | |
Mutagenesisi | 202 | D → E: Reduces activity about 98%. Increases KM for aspartate about 40-fold, enzyme is less sensitive to lysine inhibition. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000066676 | 2 – 449 | Lysine-sensitive aspartokinase 3Add BLAST | 448 |
Proteomic databases
jPOSTi | P08660 |
PaxDbi | P08660 |
PRIDEi | P08660 |
Interactioni
Subunit structurei
Homodimer. In the inactive form a homotetramer is formed.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261955, 8 interactors |
STRINGi | 511145.b4024 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
PCDDBi | P08660 |
SMRi | P08660 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08660 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 313 – 394 | ACTPROSITE-ProRule annotationAdd BLAST | 82 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 245 | AspartokinaseAdd BLAST | 244 | |
Regioni | 198 – 201 | Substrate binding | 4 | |
Regioni | 246 – 449 | InterfaceAdd BLAST | 204 | |
Regioni | 299 – 449 | Required for homodimerizationAdd BLAST | 151 | |
Regioni | 318 – 321 | Allosteric inhibitor binding 1 | 4 | |
Regioni | 324 – 325 | Allosteric inhibitor binding 1 | 2 | |
Regioni | 338 – 340 | Allosteric inhibitor binding 2 | 3 | |
Regioni | 345 – 346 | Allosteric inhibitor binding 1 | 2 |
Sequence similaritiesi
Belongs to the aspartokinase family.Curated
Phylogenomic databases
eggNOGi | COG0527, Bacteria |
HOGENOMi | CLU_009116_6_0_6 |
InParanoidi | P08660 |
PhylomeDBi | P08660 |
Family and domain databases
CDDi | cd04258, AAK_AKiii-LysC-EC, 1 hit |
Gene3Di | 1.20.120.1320, 1 hit 3.40.1160.10, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR002912, ACT_dom IPR041745, AKiii-LysC-EC IPR001048, Asp/Glu/Uridylate_kinase IPR005260, Asp_kin_monofn IPR001341, Asp_kinase IPR042199, AsparK_Bifunc_asparK/hSer_DH IPR018042, Aspartate_kinase_CS |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF01842, ACT, 1 hit |
PIRSFi | PIRSF000726, Asp_kin, 1 hit |
SUPFAMi | SSF53633, SSF53633, 1 hit |
TIGRFAMsi | TIGR00656, asp_kin_monofn, 1 hit TIGR00657, asp_kinases, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00324, ASPARTOKINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P08660-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEIVVSKFG GTSVADFDAM NRSADIVLSD ANVRLVVLSA SAGITNLLVA
60 70 80 90 100
LAEGLEPGER FEKLDAIRNI QFAILERLRY PNVIREEIER LLENITVLAE
110 120 130 140 150
AAALATSPAL TDELVSHGEL MSTLLFVEIL RERDVQAQWF DVRKVMRTND
160 170 180 190 200
RFGRAEPDIA ALAELAALQL LPRLNEGLVI TQGFIGSENK GRTTTLGRGG
210 220 230 240 250
SDYTAALLAE ALHASRVDIW TDVPGIYTTD PRVVSAAKRI DEIAFAEAAE
260 270 280 290 300
MATFGAKVLH PATLLPAVRS DIPVFVGSSK DPRAGGTLVC NKTENPPLFR
310 320 330 340 350
ALALRRNQTL LTLHSLNMLH SRGFLAEVFG ILARHNISVD LITTSEVSVA
360 370 380 390 400
LTLDTTGSTS TGDTLLTQSL LMELSALCRV EVEEGLALVA LIGNDLSKAC
410 420 430 440
GVGKEVFGVL EPFNIRMICY GASSHNLCFL VPGEDAEQVV QKLHSNLFE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 – 15 | VA → AS in CAA24910 (PubMed:6312411).Curated | 2 | |
Sequence conflicti | 20 | M → E in CAA24910 (PubMed:6312411).Curated | 1 | |
Sequence conflicti | 58 | G → C in AAA24095 (PubMed:3003049).Curated | 1 | |
Sequence conflicti | 401 | G → A in AAA24095 (PubMed:3003049).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11812 Genomic DNA Translation: AAA24095.1 U00006 Genomic DNA Translation: AAC43118.1 U00096 Genomic DNA Translation: AAC76994.1 AP009048 Genomic DNA Translation: BAE78026.1 X00008 Genomic DNA Translation: CAA24910.1 |
PIRi | G65209, KIECD3 |
RefSeqi | NP_418448.1, NC_000913.3 WP_001290310.1, NZ_SSZK01000049.1 |
Genome annotation databases
EnsemblBacteriai | AAC76994; AAC76994; b4024 BAE78026; BAE78026; BAE78026 |
GeneIDi | 57730519 948531 |
KEGGi | ecj:JW3984 eco:b4024 |
PATRICi | fig|1411691.4.peg.2689 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11812 Genomic DNA Translation: AAA24095.1 U00006 Genomic DNA Translation: AAC43118.1 U00096 Genomic DNA Translation: AAC76994.1 AP009048 Genomic DNA Translation: BAE78026.1 X00008 Genomic DNA Translation: CAA24910.1 |
PIRi | G65209, KIECD3 |
RefSeqi | NP_418448.1, NC_000913.3 WP_001290310.1, NZ_SSZK01000049.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2J0W | X-ray | 2.50 | A | 1-449 | [»] | |
2J0X | X-ray | 2.80 | A/B | 1-449 | [»] | |
PCDDBi | P08660 | |||||
SMRi | P08660 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261955, 8 interactors |
STRINGi | 511145.b4024 |
Proteomic databases
jPOSTi | P08660 |
PaxDbi | P08660 |
PRIDEi | P08660 |
Genome annotation databases
EnsemblBacteriai | AAC76994; AAC76994; b4024 BAE78026; BAE78026; BAE78026 |
GeneIDi | 57730519 948531 |
KEGGi | ecj:JW3984 eco:b4024 |
PATRICi | fig|1411691.4.peg.2689 |
Organism-specific databases
EchoBASEi | EB0545 |
Phylogenomic databases
eggNOGi | COG0527, Bacteria |
HOGENOMi | CLU_009116_6_0_6 |
InParanoidi | P08660 |
PhylomeDBi | P08660 |
Enzyme and pathway databases
UniPathwayi | UPA00034;UER00015 |
BioCyci | EcoCyc:ASPKINIII-MONOMER MetaCyc:ASPKINIII-MONOMER |
SABIO-RKi | P08660 |
Miscellaneous databases
EvolutionaryTracei | P08660 |
PROi | PR:P08660 |
Family and domain databases
CDDi | cd04258, AAK_AKiii-LysC-EC, 1 hit |
Gene3Di | 1.20.120.1320, 1 hit 3.40.1160.10, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR002912, ACT_dom IPR041745, AKiii-LysC-EC IPR001048, Asp/Glu/Uridylate_kinase IPR005260, Asp_kin_monofn IPR001341, Asp_kinase IPR042199, AsparK_Bifunc_asparK/hSer_DH IPR018042, Aspartate_kinase_CS |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF01842, ACT, 1 hit |
PIRSFi | PIRSF000726, Asp_kin, 1 hit |
SUPFAMi | SSF53633, SSF53633, 1 hit |
TIGRFAMsi | TIGR00656, asp_kin_monofn, 1 hit TIGR00657, asp_kinases, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00324, ASPARTOKINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AK3_ECOLI | |
Accessioni | P08660Primary (citable) accession number: P08660 Secondary accession number(s): Q2M6T0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | October 1, 1993 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families