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Protein

Ras-like protein 1

Gene

ras1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the process of sexual differentiation and the determination of cell shape. Essential for mating and for recognition of the mating pheromone, but not for vegetative growth. Does not regulate the intracellular cAMP level. Regulates two downstream pathways, namely the byr2/byr1/spk1 mitogen-activated protein kinase cascade and the cdc42 small G protein pathway. The former is relevant to mating and sporulation, whereas the latter is relevant to mating, cell growth and cell morphology.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi62 – 66GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4

GO - Molecular functioni

  • CTP binding Source: PomBase
  • GDP binding Source: PomBase
  • GTPase activity Source: PomBase
  • GTP binding Source: PomBase
  • UTP binding Source: PomBase

GO - Biological processi

  • Cdc42 protein signal transduction Source: PomBase
  • cell morphogenesis involved in conjugation with cellular fusion Source: PomBase
  • conjugation with cellular fusion Source: PomBase
  • establishment or maintenance of cell polarity regulating cell shape Source: PomBase
  • induction of conjugation with cellular fusion Source: PomBase
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: PomBase
  • pheromone response MAPK cascade Source: PomBase
  • positive regulation of conjugation with cellular fusion Source: PomBase
  • positive regulation of protein localization to nucleus Source: PomBase
  • protein localization to plasma membrane Source: PomBase
  • regulation of ascospore formation Source: PomBase
  • regulation of cell morphogenesis involved in conjugation with cellular fusion Source: PomBase
  • signal transduction involved in positive regulation of conjugation with cellular fusion Source: PomBase

Keywordsi

Biological processCell shape
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like protein 1
Gene namesi
Name:ras1
ORF Names:SPAC17H9.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17H9.09c
PomBaseiSPAC17H9.09c ras1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000826781 – 216Ras-like protein 1Add BLAST216
PropeptideiPRO_0000281333217 – 219Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216Cysteine methyl esterBy similarity1
Lipidationi216S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP08647
PaxDbiP08647
PRIDEiP08647

PTM databases

iPTMnetiP08647
SwissPalmiP08647

Interactioni

Subunit structurei

Scd1, scd2, cdc42, and ras1, in its GTP-bound state, act cooperatively to form a protein complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
byr2P288292EBI-15585264,EBI-1032333

Protein-protein interaction databases

BioGridi278753, 32 interactors
DIPiDIP-61182N
IntActiP08647, 2 interactors
STRINGi4896.SPAC17H9.09c.1

Structurei

3D structure databases

ProteinModelPortaliP08647
SMRiP08647
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

HOGENOMiHOG000233973
InParanoidiP08647
KOiK07827
OMAiYAQTYKL
OrthoDBiEOG092C4VY0
PhylomeDBiP08647

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSTYLREYK LVVVGDGGVG KSALTIQLIQ SHFVDEYDPT IEDSYRKKCE
60 70 80 90 100
IDGEGALLDV LDTAGQEEYS AMREQYMRTG EGFLLVYNIT SRSSFDEIST
110 120 130 140 150
FYQQILRVKD KDTFPVVLVA NKCDLEAERV VSRAEGEQLA KSMHCLYVET
160 170 180 190 200
SAKLRLNVEE AFYSLVRTIR RYNKSEEKGF QNKQAVQTAQ VPASTAKRAS
210
AVNNSKTEDE VSTKCCVIC
Length:219
Mass (Da):24,734
Last modified:July 15, 1998 - v2
Checksum:i109A0C468663F535
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6L → M in CAA27399 (PubMed:3084798).Curated1
Sequence conflicti57L → V in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti60V → L in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti81E → Q in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti134 – 136AEG → RER in CAA26191 (PubMed:4006903).Curated3
Sequence conflicti181Q → H in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti188T → I in CAA26191 (PubMed:4006903).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02331 Genomic DNA Translation: CAA26191.1
X03771 Genomic DNA Translation: CAA27399.1
CU329670 Genomic DNA Translation: CAB11218.1
PIRiA22715 TVBYSR
T37875 TVBYPR
T45545
RefSeqiNP_593579.1, NM_001019011.2

Genome annotation databases

EnsemblFungiiSPAC17H9.09c.1; SPAC17H9.09c.1:pep; SPAC17H9.09c
GeneIDi2542285
KEGGispo:SPAC17H9.09c

Similar proteinsi

Entry informationi

Entry nameiRAS_SCHPO
AccessioniPrimary (citable) accession number: P08647
Secondary accession number(s): O13806
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1998
Last modified: July 18, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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