Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTPase KRas

Gene

Kras

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (By similarity). Plays an important role in the regulation of cell proliferation (PubMed:3110778). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (By similarity).By similarity1 Publication

Activity regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18GTPBy similarity9
Nucleotide bindingi29 – 35GTPBy similarity7
Nucleotide bindingi59 – 60GTPBy similarity2
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1169092 Activation of RAS in B cells
R-RNO-1250347 SHC1 events in ERBB4 signaling
R-RNO-1433557 Signaling by SCF-KIT
R-RNO-171007 p38MAPK events
R-RNO-179812 GRB2 events in EGFR signaling
R-RNO-180336 SHC1 events in EGFR signaling
R-RNO-186763 Downstream signal transduction
R-RNO-1963640 GRB2 events in ERBB2 signaling
R-RNO-210993 Tie2 Signaling
R-RNO-2179392 EGFR Transactivation by Gastrin
R-RNO-2424491 DAP12 signaling
R-RNO-2871796 FCERI mediated MAPK activation
R-RNO-375165 NCAM signaling for neurite out-growth
R-RNO-5218921 VEGFR2 mediated cell proliferation
R-RNO-5621575 CD209 (DC-SIGN) signaling
R-RNO-5654688 SHC-mediated cascade:FGFR1
R-RNO-5654693 FRS-mediated FGFR1 signaling
R-RNO-5654699 SHC-mediated cascade:FGFR2
R-RNO-5654700 FRS-mediated FGFR2 signaling
R-RNO-5654704 SHC-mediated cascade:FGFR3
R-RNO-5654706 FRS-mediated FGFR3 signaling
R-RNO-5654712 FRS-mediated FGFR4 signaling
R-RNO-5654719 SHC-mediated cascade:FGFR4
R-RNO-5658442 Regulation of RAS by GAPs
R-RNO-5673000 RAF activation
R-RNO-5673001 RAF/MAP kinase cascade
R-RNO-5674135 MAP2K and MAPK activation
R-RNO-5675221 Negative regulation of MAPK pathway
R-RNO-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-RNO-8851805 MET activates RAS signaling
R-RNO-8951936 RUNX3 regulates p14-ARF

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase KRas
Alternative name(s):
K-Ras 2
Ki-Ras
c-K-ras
c-Ki-ras
Cleaved into the following chain:
Gene namesi
Name:Kras
Synonyms:Kras2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2981 Kras

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264831 – 186GTPase KRasAdd BLAST186
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00000826442 – 186GTPase KRas, N-terminally processedAdd BLAST185
PropeptideiPRO_0000281294187 – 189Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in GTPase KRas, N-terminally processedBy similarity1
Modified residuei104N6-acetyllysineBy similarity1
Lipidationi180S-palmitoyl cysteineBy similarity1
Modified residuei186Cysteine methyl esterBy similarity1
Lipidationi186S-farnesyl cysteineBy similarity1

Post-translational modificationi

Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PaxDbiP08644
PRIDEiP08644

PTM databases

iPTMnetiP08644
PhosphoSitePlusiP08644
SwissPalmiP08644

Expressioni

Gene expression databases

BgeeiENSRNOG00000009338 Expressed in 10 organ(s), highest expression level in lung
ExpressionAtlasiP08644 baseline and differential
GenevisibleiP08644 RN

Interactioni

Subunit structurei

Interacts with SOS1 (By similarity). Interacts (when farnesylated) with PDE6D; this promotes dissociation from the cell membrane (By similarity). Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246680, 9 interactors
STRINGi10116.ENSRNOP00000012588

Structurei

3D structure databases

ProteinModelPortaliP08644
SMRiP08644
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 185Hypervariable regionAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133672
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP08644
KOiK07827
OMAiMSKEGKK
OrthoDBiEOG091G0UAU
PhylomeDBiP08644
TreeFamiTF312796

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Isoforms differ in the C-terminal region which is encoded by two alternative exons (IVA and IVB).
Isoform 2A (identifier: P08644-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI
110 120 130 140 150
KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QELARSYGIP FIETSAKTRQ
160 170 180
RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCVIM
Length:189
Mass (Da):21,656
Last modified:July 19, 2004 - v3
Checksum:i97345422E01D2C81
GO
Isoform 2B (identifier: P08644-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-153: RVE → GVD
     165-189: QYRLKKISKEEKTPGCVKIKKCVIM → KHKEKMSKDGKKKKKKSRTRCIVM

Show »
Length:188
Mass (Da):21,495
Checksum:iB0A55318AD158F51
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12G → C in AAA42011 (PubMed:3023884).Curated1
Sequence conflicti47D → H in AAA42011 (PubMed:3023884).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011144151 – 153RVE → GVD in isoform 2B. 1 Publication3
Alternative sequenceiVSP_011145165 – 189QYRLK…KCVIM → KHKEKMSKDGKKKKKKSRTR CIVM in isoform 2B. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09793 mRNA Translation: AAB60458.1
AABR03032592 Genomic DNA No translation available.
M12260, M12259 Genomic DNA Translation: AAA42011.1
M54870 Genomic DNA Translation: AAA40937.1
M16970 Genomic DNA Translation: AAA41494.1
PIRiI58402
RefSeqiNP_113703.1, NM_031515.3 [P08644-2]
XP_008761576.1, XM_008763354.2 [P08644-2]
XP_017447931.1, XM_017592442.1 [P08644-1]
XP_017447932.1, XM_017592443.1 [P08644-1]
UniGeneiRn.24554

Genome annotation databases

EnsembliENSRNOT00000012588; ENSRNOP00000012588; ENSRNOG00000009338 [P08644-1]
ENSRNOT00000012887; ENSRNOP00000012887; ENSRNOG00000009338 [P08644-2]
GeneIDi24525
KEGGirno:24525
UCSCiRGD:2981 rat [P08644-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09793 mRNA Translation: AAB60458.1
AABR03032592 Genomic DNA No translation available.
M12260, M12259 Genomic DNA Translation: AAA42011.1
M54870 Genomic DNA Translation: AAA40937.1
M16970 Genomic DNA Translation: AAA41494.1
PIRiI58402
RefSeqiNP_113703.1, NM_031515.3 [P08644-2]
XP_008761576.1, XM_008763354.2 [P08644-2]
XP_017447931.1, XM_017592442.1 [P08644-1]
XP_017447932.1, XM_017592443.1 [P08644-1]
UniGeneiRn.24554

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4SX-ray2.60M/N/O/P/Q/R185-188[»]
ProteinModelPortaliP08644
SMRiP08644
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246680, 9 interactors
STRINGi10116.ENSRNOP00000012588

PTM databases

iPTMnetiP08644
PhosphoSitePlusiP08644
SwissPalmiP08644

Proteomic databases

PaxDbiP08644
PRIDEiP08644

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012588; ENSRNOP00000012588; ENSRNOG00000009338 [P08644-1]
ENSRNOT00000012887; ENSRNOP00000012887; ENSRNOG00000009338 [P08644-2]
GeneIDi24525
KEGGirno:24525
UCSCiRGD:2981 rat [P08644-1]

Organism-specific databases

CTDi3845
RGDi2981 Kras

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133672
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP08644
KOiK07827
OMAiMSKEGKK
OrthoDBiEOG091G0UAU
PhylomeDBiP08644
TreeFamiTF312796

Enzyme and pathway databases

ReactomeiR-RNO-1169092 Activation of RAS in B cells
R-RNO-1250347 SHC1 events in ERBB4 signaling
R-RNO-1433557 Signaling by SCF-KIT
R-RNO-171007 p38MAPK events
R-RNO-179812 GRB2 events in EGFR signaling
R-RNO-180336 SHC1 events in EGFR signaling
R-RNO-186763 Downstream signal transduction
R-RNO-1963640 GRB2 events in ERBB2 signaling
R-RNO-210993 Tie2 Signaling
R-RNO-2179392 EGFR Transactivation by Gastrin
R-RNO-2424491 DAP12 signaling
R-RNO-2871796 FCERI mediated MAPK activation
R-RNO-375165 NCAM signaling for neurite out-growth
R-RNO-5218921 VEGFR2 mediated cell proliferation
R-RNO-5621575 CD209 (DC-SIGN) signaling
R-RNO-5654688 SHC-mediated cascade:FGFR1
R-RNO-5654693 FRS-mediated FGFR1 signaling
R-RNO-5654699 SHC-mediated cascade:FGFR2
R-RNO-5654700 FRS-mediated FGFR2 signaling
R-RNO-5654704 SHC-mediated cascade:FGFR3
R-RNO-5654706 FRS-mediated FGFR3 signaling
R-RNO-5654712 FRS-mediated FGFR4 signaling
R-RNO-5654719 SHC-mediated cascade:FGFR4
R-RNO-5658442 Regulation of RAS by GAPs
R-RNO-5673000 RAF activation
R-RNO-5673001 RAF/MAP kinase cascade
R-RNO-5674135 MAP2K and MAPK activation
R-RNO-5675221 Negative regulation of MAPK pathway
R-RNO-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-RNO-8851805 MET activates RAS signaling
R-RNO-8951936 RUNX3 regulates p14-ARF

Miscellaneous databases

PROiPR:P08644

Gene expression databases

BgeeiENSRNOG00000009338 Expressed in 10 organ(s), highest expression level in lung
ExpressionAtlasiP08644 baseline and differential
GenevisibleiP08644 RN

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRASK_RAT
AccessioniPrimary (citable) accession number: P08644
Secondary accession number(s): P46203, P97914
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 19, 2004
Last modified: November 7, 2018
This is version 177 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again