UniProtKB - P08644 (RASK_RAT)
GTPase KRas
Kras
Functioni
Catalytic activityi
- EC:3.6.5.2By similarity
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | GTPBy similarity | 9 | |
Nucleotide bindingi | 29 – 35 | GTPBy similarity | 7 | |
Nucleotide bindingi | 59 – 60 | GTPBy similarity | 2 | |
Nucleotide bindingi | 116 – 119 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: RGD
- GMP binding Source: RGD
- GTPase activity Source: RGD
- GTP binding Source: GO_Central
- identical protein binding Source: RGD
- LRR domain binding Source: RGD
- protein-containing complex binding Source: RGD
GO - Biological processi
- actin cytoskeleton organization Source: RGD
- cytokine-mediated signaling pathway Source: RGD
- endocrine signaling Source: RGD
- epithelial tube branching involved in lung morphogenesis Source: RGD
- female pregnancy Source: RGD
- forebrain astrocyte development Source: RGD
- homeostasis of number of cells within a tissue Source: RGD
- liver development Source: RGD
- negative regulation of cell differentiation Source: RGD
- negative regulation of neuron apoptotic process Source: RGD
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of cellular senescence Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of MAP kinase activity Source: RGD
- positive regulation of NF-kappaB transcription factor activity Source: RGD
- positive regulation of nitric-oxide synthase activity Source: RGD
- positive regulation of protein phosphorylation Source: RGD
- positive regulation of Rac protein signal transduction Source: RGD
- Ras protein signal transduction Source: RGD
- regulation of long-term neuronal synaptic plasticity Source: RGD
- regulation of protein stability Source: UniProtKB
- regulation of synaptic transmission, GABAergic Source: RGD
- response to glucocorticoid Source: RGD
- response to isolation stress Source: RGD
- response to mineralocorticoid Source: RGD
- striated muscle cell differentiation Source: RGD
- visual learning Source: RGD
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-1169092, Activation of RAS in B cells R-RNO-1250347, SHC1 events in ERBB4 signaling R-RNO-1433557, Signaling by SCF-KIT R-RNO-171007, p38MAPK events R-RNO-179812, GRB2 events in EGFR signaling R-RNO-180336, SHC1 events in EGFR signaling R-RNO-186763, Downstream signal transduction R-RNO-1963640, GRB2 events in ERBB2 signaling R-RNO-210993, Tie2 Signaling R-RNO-2179392, EGFR Transactivation by Gastrin R-RNO-2424491, DAP12 signaling R-RNO-2871796, FCERI mediated MAPK activation R-RNO-375165, NCAM signaling for neurite out-growth R-RNO-4086398, Ca2+ pathway R-RNO-5218921, VEGFR2 mediated cell proliferation R-RNO-5621575, CD209 (DC-SIGN) signaling R-RNO-5654688, SHC-mediated cascade:FGFR1 R-RNO-5654693, FRS-mediated FGFR1 signaling R-RNO-5654699, SHC-mediated cascade:FGFR2 R-RNO-5654700, FRS-mediated FGFR2 signaling R-RNO-5654704, SHC-mediated cascade:FGFR3 R-RNO-5654706, FRS-mediated FGFR3 signaling R-RNO-5654712, FRS-mediated FGFR4 signaling R-RNO-5654719, SHC-mediated cascade:FGFR4 R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5673000, RAF activation R-RNO-5673001, RAF/MAP kinase cascade R-RNO-5674135, MAP2K and MAPK activation R-RNO-5675221, Negative regulation of MAPK pathway R-RNO-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-RNO-8851805, MET activates RAS signaling R-RNO-9607240, FLT3 Signaling R-RNO-9634635, Estrogen-stimulated signaling through PRKCZ R-RNO-9648002, RAS processing |
Names & Taxonomyi
Protein namesi | Recommended name: GTPase KRas (EC:3.6.5.2By similarity)Alternative name(s): K-Ras 2 Ki-Ras c-K-ras c-Ki-ras Cleaved into the following chain: |
Gene namesi | Name:Kras Synonyms:Kras2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2981, Kras |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Cytosol
- cytosol By similarity
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Mitochondrion
- mitochondrion Source: RGD
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- plasma membrane Source: RGD
Other locations
- cytoplasm Source: RGD
- membrane Source: RGD
- membrane raft Source: RGD
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000326483 | 1 – 186 | GTPase KRasAdd BLAST | 186 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000082644 | 2 – 186 | GTPase KRas, N-terminally processedAdd BLAST | 185 | |
PropeptideiPRO_0000281294 | 187 – 189 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylthreonine; in GTPase KRas, N-terminally processedBy similarity | 1 | |
Modified residuei | 104 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 170 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Lipidationi | 180 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 186 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 186 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Lipoprotein, Methylation, Palmitate, Prenylation, Ubl conjugationProteomic databases
jPOSTi | P08644 |
PaxDbi | P08644 |
PRIDEi | P08644 |
PTM databases
iPTMneti | P08644 |
PhosphoSitePlusi | P08644 |
SwissPalmi | P08644 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000009338, Expressed in duodenum and 21 other tissues |
ExpressionAtlasi | P08644, baseline and differential |
Genevisiblei | P08644, RN |
Interactioni
Subunit structurei
Interacts with SOS1 (By similarity).
Interacts (when farnesylated) with PDE6D; this promotes dissociation from the cell membrane (By similarity).
Interacts with PHLPP.
Interacts (active GTP-bound form preferentially) with RGS14.
Interacts with (when farnesylated) with GPR31 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: RGD
- LRR domain binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 246680, 11 interactors |
STRINGi | 10116.ENSRNOP00000012588 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 166 – 185 | Hypervariable regionAdd BLAST | 20 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector region | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155871 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P08644 |
OMAi | EYKLVVM |
OrthoDBi | 1259506at2759 |
PhylomeDBi | P08644 |
TreeFami | TF312796 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI
110 120 130 140 150
KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QELARSYGIP FIETSAKTRQ
160 170 180
RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCVIM
The sequence of this isoform differs from the canonical sequence as follows:
151-153: RVE → GVD
165-189: QYRLKKISKEEKTPGCVKIKKCVIM → KHKEKMSKDGKKKKKKSRTRCIVM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | G → C in AAA42011 (PubMed:3023884).Curated | 1 | |
Sequence conflicti | 47 | D → H in AAA42011 (PubMed:3023884).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011144 | 151 – 153 | RVE → GVD in isoform 2B. 1 Publication | 3 | |
Alternative sequenceiVSP_011145 | 165 – 189 | QYRLK…KCVIM → KHKEKMSKDGKKKKKKSRTR CIVM in isoform 2B. 1 PublicationAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09793 mRNA Translation: AAB60458.1 AABR03032592 Genomic DNA No translation available. M12260, M12259 Genomic DNA Translation: AAA42011.1 M54870 Genomic DNA Translation: AAA40937.1 M16970 Genomic DNA Translation: AAA41494.1 |
PIRi | I58402 |
RefSeqi | NP_113703.1, NM_031515.3 [P08644-2] XP_008761576.1, XM_008763354.2 [P08644-2] XP_017447931.1, XM_017592442.1 [P08644-1] XP_017447932.1, XM_017592443.1 [P08644-1] |
Genome annotation databases
Ensembli | ENSRNOT00000012588; ENSRNOP00000012588; ENSRNOG00000009338 [P08644-1] ENSRNOT00000012887; ENSRNOP00000012887; ENSRNOG00000009338 [P08644-2] |
GeneIDi | 24525 |
KEGGi | rno:24525 |
UCSCi | RGD:2981, rat [P08644-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09793 mRNA Translation: AAB60458.1 AABR03032592 Genomic DNA No translation available. M12260, M12259 Genomic DNA Translation: AAA42011.1 M54870 Genomic DNA Translation: AAA40937.1 M16970 Genomic DNA Translation: AAA41494.1 |
PIRi | I58402 |
RefSeqi | NP_113703.1, NM_031515.3 [P08644-2] XP_008761576.1, XM_008763354.2 [P08644-2] XP_017447931.1, XM_017592442.1 [P08644-1] XP_017447932.1, XM_017592443.1 [P08644-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N4S | X-ray | 2.60 | M/N/O/P/Q/R | 185-188 | [»] | |
BMRBi | P08644 | |||||
SMRi | P08644 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 246680, 11 interactors |
STRINGi | 10116.ENSRNOP00000012588 |
PTM databases
iPTMneti | P08644 |
PhosphoSitePlusi | P08644 |
SwissPalmi | P08644 |
Proteomic databases
jPOSTi | P08644 |
PaxDbi | P08644 |
PRIDEi | P08644 |
Genome annotation databases
Ensembli | ENSRNOT00000012588; ENSRNOP00000012588; ENSRNOG00000009338 [P08644-1] ENSRNOT00000012887; ENSRNOP00000012887; ENSRNOG00000009338 [P08644-2] |
GeneIDi | 24525 |
KEGGi | rno:24525 |
UCSCi | RGD:2981, rat [P08644-1] |
Organism-specific databases
CTDi | 3845 |
RGDi | 2981, Kras |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155871 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P08644 |
OMAi | EYKLVVM |
OrthoDBi | 1259506at2759 |
PhylomeDBi | P08644 |
TreeFami | TF312796 |
Enzyme and pathway databases
Reactomei | R-RNO-1169092, Activation of RAS in B cells R-RNO-1250347, SHC1 events in ERBB4 signaling R-RNO-1433557, Signaling by SCF-KIT R-RNO-171007, p38MAPK events R-RNO-179812, GRB2 events in EGFR signaling R-RNO-180336, SHC1 events in EGFR signaling R-RNO-186763, Downstream signal transduction R-RNO-1963640, GRB2 events in ERBB2 signaling R-RNO-210993, Tie2 Signaling R-RNO-2179392, EGFR Transactivation by Gastrin R-RNO-2424491, DAP12 signaling R-RNO-2871796, FCERI mediated MAPK activation R-RNO-375165, NCAM signaling for neurite out-growth R-RNO-4086398, Ca2+ pathway R-RNO-5218921, VEGFR2 mediated cell proliferation R-RNO-5621575, CD209 (DC-SIGN) signaling R-RNO-5654688, SHC-mediated cascade:FGFR1 R-RNO-5654693, FRS-mediated FGFR1 signaling R-RNO-5654699, SHC-mediated cascade:FGFR2 R-RNO-5654700, FRS-mediated FGFR2 signaling R-RNO-5654704, SHC-mediated cascade:FGFR3 R-RNO-5654706, FRS-mediated FGFR3 signaling R-RNO-5654712, FRS-mediated FGFR4 signaling R-RNO-5654719, SHC-mediated cascade:FGFR4 R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5673000, RAF activation R-RNO-5673001, RAF/MAP kinase cascade R-RNO-5674135, MAP2K and MAPK activation R-RNO-5675221, Negative regulation of MAPK pathway R-RNO-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-RNO-8851805, MET activates RAS signaling R-RNO-9607240, FLT3 Signaling R-RNO-9634635, Estrogen-stimulated signaling through PRKCZ R-RNO-9648002, RAS processing |
Miscellaneous databases
PROi | PR:P08644 |
Gene expression databases
Bgeei | ENSRNOG00000009338, Expressed in duodenum and 21 other tissues |
ExpressionAtlasi | P08644, baseline and differential |
Genevisiblei | P08644, RN |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RASK_RAT | |
Accessioni | P08644Primary (citable) accession number: P08644 Secondary accession number(s): P46203, P97914 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | July 19, 2004 | |
Last modified: | December 2, 2020 | |
This is version 192 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families