Regulatory protein LEU3

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• DNA-binding transcription activator activity, RNA polymerase II-specific Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "In vitro transcriptional activation by a metabolic intermediate: activation by Leu3 depends on alpha-isopropylmalate."
    Sze J.Y., Woontner M., Jaehning J.A., Kohlhaw G.B.
    Science 258:1143-1145(1992) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription repressor activity, RNA polymerase II-specific Source: SGDInferred from direct assayⁱ
  • "In vitro transcriptional activation by a metabolic intermediate: activation by Leu3 depends on alpha-isopropylmalate."
    Sze J.Y., Woontner M., Jaehning J.A., Kohlhaw G.B.
    Science 258:1143-1145(1992) [PubMed] [Europe PMC] [Abstract]
• sequence-specific DNA binding Source: SGDInferred from high throughput direct assayⁱ
  • "A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters."
    Badis G., Chan E.T., van Bakel H., Pena-Castillo L., Tillo D., Tsui K., Carlson C.D., Gossett A.J., Hasinoff M.J., Warren C.L., Gebbia M., Talukder S., Yang A., Mnaimneh S., Terterov D., Coburn D., Li Yeo A., Yeo Z.X.

    , Clarke N.D., Lieb J.D., Ansari A.Z., Nislow C., Hughes T.R.
    Mol. Cell 32:878-887(2008) [PubMed] [Europe PMC] [Abstract]
• transcription regulatory region sequence-specific DNA binding Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• zinc ion binding Source: InterPro

GO - Biological processⁱ

• leucine biosynthetic process Source: UniProtKB-KW
• negative regulation of transcription by RNA polymerase II Source: SGDInferred from direct assayⁱ
  • "In vitro transcriptional activation by a metabolic intermediate: activation by Leu3 depends on alpha-isopropylmalate."
    Sze J.Y., Woontner M., Jaehning J.A., Kohlhaw G.B.
    Science 258:1143-1145(1992) [PubMed] [Europe PMC] [Abstract]
• positive regulation of leucine biosynthetic process Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • Ref.2

    "LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes."
    Friden P., Schimmel P.
    Mol. Cell. Biol. 7:2708-2717(1987) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• positive regulation of transcription by RNA polymerase II Source: SGDInferred from direct assayⁱ
  • "In vitro transcriptional activation by a metabolic intermediate: activation by Leu3 depends on alpha-isopropylmalate."
    Sze J.Y., Woontner M., Jaehning J.A., Kohlhaw G.B.
    Science 258:1143-1145(1992) [PubMed] [Europe PMC] [Abstract]
  • "Yeast regulatory protein LEU3: a structure-function analysis."
    Zhou K.M., Bai Y.L., Kohlhaw G.B.
    Nucleic Acids Res 18:291-298(1990) [PubMed] [Europe PMC] [Abstract]
• regulation of transcription, DNA-templated Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Motif

Compositional bias

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MEGRSDFVAT SQSGSEMSHS ETRNRTGMNA RKRKFACVEC RQQKSKCDAH 
        60         70         80         90        100
ERAPEPCTKC AKKNVPCILK RDFRRTYKRA RNEAIEKRFK ELTRTLTNLT 
       110        120        130        140        150
SDEILKKIEE EQEIVLDNSN FTKEKVKQLR KSAFETTEIE PRSYKTLRGE 
       160        170        180        190        200
PISYSTNRRH TDSSPLTLLS SSTNFDPVHS TNVMTDDQLK CLPKSLGDVY 
       210        220        230        240        250
LSSSDIAELF QEFATKYHQF LPVVDLSKGA ERIYHLSPCL FWVILLIGLR 
       260        270        280        290        300
RKFGATDLMT RLSVLVKSVL SEITISPIIR YTPSDKDEPV LNVASVYSVQ 
       310        320        330        340        350
AFLLYTFWPP LTSSLSADTS WNTIGTAMFQ ALRVGLNCAG FSKEYASANS 
       360        370        380        390        400
ELVNEQIRTW ICCNVVSQTV ASSFGFPAYV SFDYLVISSI RVPNSKSQVD 
       410        420        430        440        450
IPNELRQMAQ IARFENQIVN TMNSTPASVT GMVSQEEKQP LLHVLNQQLS 
       460        470        480        490        500
QLEISLEENN LDDIRKFLLL VAKVHLLTYY FTDVTSQSAG KSNGNIYEGS 
       510        520        530        540        550
YSIMELDTSF ETKRGLVKVY NAAVNFLIHA NSMWEHDPTI IKYFPGLFVL 
       560        570        580        590        600
NIWQSACIIS KLIHSSLHSM LDVNSGKKAY NNAISLTFNA SVLKYDMAYR 
       610        620        630        640        650
SSGIMRSIWS LFANMYDAWK NDQKEGGGRL NNDFNLGITI KSRMSVNVFF 
       660        670        680        690        700
DCLYILKEKC GMAKLERETK VSTAYNVDEE EEEDEDEEGE EEEEEEELSS 
       710        720        730        740        750
KVPENMDSQQ LRTRKFTNVR HPEKKARKII ETIPLDPNPI NAGSTSSGSS 
       760        770        780        790        800
LTTPNSQVAN TISYRGILNK MSPREQLNHA NLDSSVSTDI KDTEAVNEPL 
       810        820        830        840        850
PIGRNAEHPA NQPPLSITQM QENTLPATQA NSSLLETYPI VQSNPVTTTI 
       860        870        880 
KESPNSIMAG WDNWESDMVW RDVDILMNEF AFNPKV                

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
2. Yeast chromosome XII
   Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
3. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references