UniProtKB - P08638 (LEUR_YEAST)
Protein
Regulatory protein LEU3
Gene
LEU3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Factor for control of RNA levels of a group of leucine-specific genes.
Miscellaneous
Present with 125 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 37 – 67 | Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST | 31 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: SGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: SGD
- sequence-specific DNA binding Source: SGD
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- leucine biosynthetic process Source: UniProtKB-KW
- negative regulation of transcription by RNA polymerase II Source: SGD
- positive regulation of leucine biosynthetic process Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Regulatory protein LEU3 |
Gene namesi | Name:LEU3 Ordered Locus Names:YLR451W ORF Names:L9324.1 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004443, LEU3 |
VEuPathDBi | FungiDB:YLR451W |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000114954 | 1 – 886 | Regulatory protein LEU3Add BLAST | 886 |
Proteomic databases
MaxQBi | P08638 |
PaxDbi | P08638 |
PRIDEi | P08638 |
PTM databases
iPTMneti | P08638 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 31709, 69 interactors |
DIPi | DIP-2400N |
IntActi | P08638, 2 interactors |
MINTi | P08638 |
STRINGi | 4932.YLR451W |
Miscellaneous databases
RNActi | P08638, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08638 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08638 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 874 – 882 | 9aaTAD | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 678 – 697 | Asp/Glu-rich (acidic)Add BLAST | 20 |
Domaini
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication
Phylogenomic databases
eggNOGi | ENOG502QPVP, Eukaryota |
GeneTreei | ENSGT00940000176683 |
HOGENOMi | CLU_015609_0_0_1 |
InParanoidi | P08638 |
OMAi | VPQDYRV |
Family and domain databases
CDDi | cd00067, GAL4, 1 hit |
Gene3Di | 4.10.240.10, 1 hit |
InterProi | View protein in InterPro IPR001138, Zn2-C6_fun-type_DNA-bd IPR036864, Zn2-C6_fun-type_DNA-bd_sf |
Pfami | View protein in Pfam PF00172, Zn_clus, 1 hit |
SMARTi | View protein in SMART SM00066, GAL4, 1 hit |
SUPFAMi | SSF57701, SSF57701, 1 hit |
PROSITEi | View protein in PROSITE PS00463, ZN2_CY6_FUNGAL_1, 1 hit PS50048, ZN2_CY6_FUNGAL_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P08638-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEGRSDFVAT SQSGSEMSHS ETRNRTGMNA RKRKFACVEC RQQKSKCDAH
60 70 80 90 100
ERAPEPCTKC AKKNVPCILK RDFRRTYKRA RNEAIEKRFK ELTRTLTNLT
110 120 130 140 150
SDEILKKIEE EQEIVLDNSN FTKEKVKQLR KSAFETTEIE PRSYKTLRGE
160 170 180 190 200
PISYSTNRRH TDSSPLTLLS SSTNFDPVHS TNVMTDDQLK CLPKSLGDVY
210 220 230 240 250
LSSSDIAELF QEFATKYHQF LPVVDLSKGA ERIYHLSPCL FWVILLIGLR
260 270 280 290 300
RKFGATDLMT RLSVLVKSVL SEITISPIIR YTPSDKDEPV LNVASVYSVQ
310 320 330 340 350
AFLLYTFWPP LTSSLSADTS WNTIGTAMFQ ALRVGLNCAG FSKEYASANS
360 370 380 390 400
ELVNEQIRTW ICCNVVSQTV ASSFGFPAYV SFDYLVISSI RVPNSKSQVD
410 420 430 440 450
IPNELRQMAQ IARFENQIVN TMNSTPASVT GMVSQEEKQP LLHVLNQQLS
460 470 480 490 500
QLEISLEENN LDDIRKFLLL VAKVHLLTYY FTDVTSQSAG KSNGNIYEGS
510 520 530 540 550
YSIMELDTSF ETKRGLVKVY NAAVNFLIHA NSMWEHDPTI IKYFPGLFVL
560 570 580 590 600
NIWQSACIIS KLIHSSLHSM LDVNSGKKAY NNAISLTFNA SVLKYDMAYR
610 620 630 640 650
SSGIMRSIWS LFANMYDAWK NDQKEGGGRL NNDFNLGITI KSRMSVNVFF
660 670 680 690 700
DCLYILKEKC GMAKLERETK VSTAYNVDEE EEEDEDEEGE EEEEEEELSS
710 720 730 740 750
KVPENMDSQQ LRTRKFTNVR HPEKKARKII ETIPLDPNPI NAGSTSSGSS
760 770 780 790 800
LTTPNSQVAN TISYRGILNK MSPREQLNHA NLDSSVSTDI KDTEAVNEPL
810 820 830 840 850
PIGRNAEHPA NQPPLSITQM QENTLPATQA NSSLLETYPI VQSNPVTTTI
860 870 880
KESPNSIMAG WDNWESDMVW RDVDILMNEF AFNPKV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 504 | M → I in AAA34741 (PubMed:2823102).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00360 Genomic DNA Translation: CAA68438.1 M17222 Genomic DNA Translation: AAA34741.1 U22382 Genomic DNA Translation: AAB67526.1 BK006945 Genomic DNA Translation: DAA09751.1 |
PIRi | S00638, RGBYL3 |
RefSeqi | NP_013556.3, NM_001182339.3 |
Genome annotation databases
EnsemblFungii | YLR451W_mRNA; YLR451W; YLR451W |
GeneIDi | 851172 |
KEGGi | sce:YLR451W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00360 Genomic DNA Translation: CAA68438.1 M17222 Genomic DNA Translation: AAA34741.1 U22382 Genomic DNA Translation: AAB67526.1 BK006945 Genomic DNA Translation: DAA09751.1 |
PIRi | S00638, RGBYL3 |
RefSeqi | NP_013556.3, NM_001182339.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ER8 | X-ray | 2.85 | A/B/C/D | 32-103 | [»] | |
2ERE | X-ray | 3.00 | A/B | 32-103 | [»] | |
2ERG | X-ray | 3.15 | A/B | 32-103 | [»] | |
SMRi | P08638 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31709, 69 interactors |
DIPi | DIP-2400N |
IntActi | P08638, 2 interactors |
MINTi | P08638 |
STRINGi | 4932.YLR451W |
PTM databases
iPTMneti | P08638 |
Proteomic databases
MaxQBi | P08638 |
PaxDbi | P08638 |
PRIDEi | P08638 |
Genome annotation databases
EnsemblFungii | YLR451W_mRNA; YLR451W; YLR451W |
GeneIDi | 851172 |
KEGGi | sce:YLR451W |
Organism-specific databases
SGDi | S000004443, LEU3 |
VEuPathDBi | FungiDB:YLR451W |
Phylogenomic databases
eggNOGi | ENOG502QPVP, Eukaryota |
GeneTreei | ENSGT00940000176683 |
HOGENOMi | CLU_015609_0_0_1 |
InParanoidi | P08638 |
OMAi | VPQDYRV |
Miscellaneous databases
EvolutionaryTracei | P08638 |
PROi | PR:P08638 |
RNActi | P08638, protein |
Family and domain databases
CDDi | cd00067, GAL4, 1 hit |
Gene3Di | 4.10.240.10, 1 hit |
InterProi | View protein in InterPro IPR001138, Zn2-C6_fun-type_DNA-bd IPR036864, Zn2-C6_fun-type_DNA-bd_sf |
Pfami | View protein in Pfam PF00172, Zn_clus, 1 hit |
SMARTi | View protein in SMART SM00066, GAL4, 1 hit |
SUPFAMi | SSF57701, SSF57701, 1 hit |
PROSITEi | View protein in PROSITE PS00463, ZN2_CY6_FUNGAL_1, 1 hit PS50048, ZN2_CY6_FUNGAL_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEUR_YEAST | |
Accessioni | P08638Primary (citable) accession number: P08638 Secondary accession number(s): D6VZ85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references