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Protein

U1 small nuclear ribonucleoprotein 70 kDa

Gene

SNRNP70

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.

Miscellaneous

Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • RNA binding Source: UniProtKB
  • U1 snRNA binding Source: GO_Central
  • U1 snRNP binding Source: Ensembl

GO - Biological processi

  • cellular response to retinoic acid Source: Ensembl
  • cellular response to transforming growth factor beta stimulus Source: Ensembl
  • cellular response to tumor necrosis factor Source: Ensembl
  • mRNA processing Source: ProtInc
  • mRNA splicing, via spliceosome Source: UniProtKB
  • negative regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • negative regulation of protein refolding Source: ParkinsonsUK-UCL
  • positive regulation of mRNA splicing, via spliceosome Source: Ensembl
  • regulation of ATPase activity Source: ParkinsonsUK-UCL
  • regulation of RNA splicing Source: UniProtKB
  • RNA splicing Source: ProtInc

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Protein family/group databases

MoonDBiP08621 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein 70 kDa
Short name:
U1 snRNP 70 kDa
Short name:
U1-70K
Short name:
snRNP70
Gene namesi
Name:SNRNP70
Synonyms:RNPU1Z, RPU1, SNRP70, U1AP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000104852.14
HGNCiHGNC:11150 SNRNP70
MIMi180740 gene
neXtProtiNX_P08621

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6625
OpenTargetsiENSG00000104852
PharmGKBiPA35992

Polymorphism and mutation databases

BioMutaiSNRNP70
DMDMi13635663

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000818802 – 437U1 small nuclear ribonucleoprotein 70 kDaAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei126PhosphotyrosineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei320PhosphoserineCombined sources1
Cross-linki346Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei410PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP08621
MaxQBiP08621
PaxDbiP08621
PeptideAtlasiP08621
PRIDEiP08621
ProteomicsDBi52138
52139 [P08621-2]
52140 [P08621-3]
52141 [P08621-4]

PTM databases

iPTMnetiP08621
PhosphoSitePlusiP08621
SwissPalmiP08621

Miscellaneous databases

PMAP-CutDBiP08621

Expressioni

Gene expression databases

BgeeiENSG00000104852
ExpressionAtlasiP08621 baseline and differential
GenevisibleiP08621 HS

Organism-specific databases

HPAiCAB001718
HPA042050
HPA043516

Interactioni

Subunit structurei

U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C (PubMed:19325628). Interacts with SCNM1 (By similarity). Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2 (PubMed:9531537). Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10 (PubMed:10339552). Interacts with dephosphorylated SFRS13A and SFPQ (PubMed:11514619, PubMed:14765198). Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2 (PubMed:14561889, PubMed:11448987, PubMed:9447963). Interacts with GEMIN5 (PubMed:25911097).By similarity9 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi112509, 215 interactors
CORUMiP08621
DIPiDIP-29406N
ELMiP08621
IntActiP08621, 78 interactors
MINTiP08621
STRINGi9606.ENSP00000472998

Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi29 – 31Combined sources3
Helixi42 – 47Combined sources6
Helixi51 – 53Combined sources3
Helixi63 – 86Combined sources24
Helixi91 – 93Combined sources3
Helixi101 – 103Combined sources3
Beta strandi104 – 109Combined sources6
Helixi116 – 124Combined sources9
Beta strandi129 – 136Combined sources8
Turni138 – 140Combined sources3
Beta strandi143 – 153Combined sources11
Helixi154 – 163Combined sources10
Beta strandi175 – 178Combined sources4
Turni181 – 183Combined sources3
Helixi190 – 192Combined sources3

3D structure databases

ProteinModelPortaliP08621
SMRiP08621
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08621

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 181RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi231 – 310Arg/Glu-rich (mixed charge)Add BLAST80
Compositional biasi311 – 314Poly-Gly4
Compositional biasi344 – 393Arg/Asp/Glu-rich (mixed charge)Add BLAST50
Compositional biasi394 – 398Poly-Gly5

Phylogenomic databases

eggNOGiKOG0113 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00530000063750
InParanoidiP08621
KOiK11093
OMAiNDVNIKH
OrthoDBiEOG091G0R0Z
PhylomeDBiP08621
TreeFamiTF314215

Family and domain databases

CDDicd12236 RRM_snRNP70, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034143 snRNP70_RRM
IPR022023 U1snRNP70_N
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF12220 U1snRNP70_N, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P08621-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED
60 70 80 90 100
PRDAPPPTRA ETREERMERK RREKIERRQQ EVETELKMWD PHNDPNAQGD
110 120 130 140 150
AFKTLFVARV NYDTTESKLR REFEVYGPIK RIHMVYSKRS GKPRGYAFIE
160 170 180 190 200
YEHERDMHSA YKHADGKKID GRRVLVDVER GRTVKGWRPR RLGGGLGGTR
210 220 230 240 250
RGGADVNIRH SGRDDTSRYD ERPGPSPLPH RDRDRDRERE RRERSRERDK
260 270 280 290 300
ERERRRSRSR DRRRRSRSRD KEERRRSRER SKDKDRDRKR RSSRSRERAR
310 320 330 340 350
RERERKEELR GGGGDMAEPS EAGDAPPDDG PPGELGPDGP DGPEEKGRDR
360 370 380 390 400
DRERRRSHRS ERERRRDRDR DRDRDREHKR GERGSERGRD EARGGGGGQD
410 420 430
NGLEGLGNDS RDMYMESEGG DGYLAPENGY LMEAAPE
Length:437
Mass (Da):51,557
Last modified:August 1, 1988 - v2
Checksum:iF1020BF5C40CF97D
GO
Isoform 2 (identifier: P08621-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-231: Missing.

Show »
Length:428
Mass (Da):50,618
Checksum:iEEBC1CE4E8B9DBDA
GO
Isoform 3 (identifier: P08621-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-166: AYKHADG → TTQLACS
     167-437: Missing.

Show »
Length:166
Mass (Da):19,790
Checksum:iE90F034A08D3E79C
GO
Isoform 4 (identifier: P08621-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: MTQFLPPNLL...EYEHERDMHS → MEQALHRFGR...PLPLPTSVTA

Show »
Length:341
Mass (Da):39,191
Checksum:i390967CC272E50E9
GO

Sequence cautioni

The sequence CAA28352 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA29964 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA29966 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0058471 – 159MTQFL…RDMHS → MEQALHRFGRGLVWLSVAWL SVGRVRVRDDGDTGRGFCRA GPVLTRGPSGDSSPLPLPTS VTA in isoform 4. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_005848160 – 166AYKHADG → TTQLACS in isoform 3. 1 Publication7
Alternative sequenceiVSP_005849167 – 437Missing in isoform 3. 1 PublicationAdd BLAST271
Alternative sequenceiVSP_005850223 – 231Missing in isoform 2. Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04654 mRNA Translation: CAA28352.1 Different initiation.
X06814 mRNA Translation: CAA29963.1
X06815 mRNA Translation: CAA29964.1 Different initiation.
X06817 mRNA Translation: CAA29966.1 Different initiation.
X07402 mRNA Translation: CAA30304.1
X06816 mRNA Translation: CAA29965.1
X06812 mRNA Translation: CAA29961.1
X06811 mRNA Translation: CAA29960.1
X07401 mRNA Translation: CAA30303.1
X07403 mRNA Translation: CAA30305.1
M22636 mRNA Translation: AAA03001.1
M57939
, M57929, M57930, M57932, M57934, M57937 Genomic DNA Translation: AAA36572.1
M57939
, M57929, M57930, M57932, M57934, M57937 Genomic DNA Translation: AAA36573.1
M57935
, M57929, M57930, M57932, M57934 Genomic DNA Translation: AAA36571.1
AK096783 mRNA Translation: BAG53365.1
X84841 mRNA Translation: CAA59278.1
AL117507 mRNA Translation: CAB55969.1
CH471177 Genomic DNA Translation: EAW52449.1
BC001315 mRNA Translation: AAH01315.1
CCDSiCCDS12756.1 [P08621-1]
CCDS74417.1 [P08621-2]
PIRiA25707
S00674
RefSeqiNP_001287998.1, NM_001301069.1 [P08621-2]
NP_003080.2, NM_003089.5 [P08621-1]
UniGeneiHs.467097

Genome annotation databases

EnsembliENST00000221448; ENSP00000221448; ENSG00000104852 [P08621-2]
ENST00000401730; ENSP00000385077; ENSG00000104852 [P08621-3]
ENST00000595231; ENSP00000471006; ENSG00000104852 [P08621-3]
ENST00000598441; ENSP00000472998; ENSG00000104852 [P08621-1]
ENST00000601065; ENSP00000468952; ENSG00000104852 [P08621-3]
GeneIDi6625
KEGGihsa:6625
UCSCiuc002pmk.4 human [P08621-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRU17_HUMAN
AccessioniPrimary (citable) accession number: P08621
Secondary accession number(s): B3KUA3
, P78493, P78494, Q15364, Q15686, Q15687, Q15689, Q99377, Q9UE45, Q9UE46, Q9UE47, Q9UE48, Q9UFQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 18, 2018
This is version 210 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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