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Protein

Monocyte differentiation antigen CD14

Gene

CD14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).6 Publications

GO - Molecular functioni

  • lipopeptide binding Source: AgBase
  • lipopolysaccharide binding Source: MGI
  • lipoteichoic acid binding Source: MGI
  • opsonin receptor activity Source: BHF-UCL
  • peptidoglycan receptor activity Source: ProtInc

GO - Biological processi

Keywordsi

Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-HSA-1236974 ER-Phagosome pathway
R-HSA-140534 via Death Receptors in the presence of ligand
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade
R-HSA-166020 Transfer of LPS from LBP carrier to CD14
R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane
R-HSA-166166 MyD88-independent TLR4 cascade
R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade
R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade
R-HSA-2562578 TRIF-mediated programmed cell death
R-HSA-5602498 MyD88 deficiency (TLR2/4)
R-HSA-5603041 IRAK4 deficiency (TLR2/4)
R-HSA-5686938 Regulation of TLR by endogenous ligand
R-HSA-6798695 Neutrophil degranulation
R-HSA-9014325 TICAM1,TRAF6-dependent induction of TAK1 complex
R-HSA-936964 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
R-HSA-937041 IKK complex recruitment mediated by RIP1
R-HSA-937072 TRAF6-mediated induction of TAK1 complex within TLR4 complex
R-HSA-975163 IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation
SIGNORiP08571

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD141 Publication
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Cleaved into the following 2 chains:
Gene namesi
Name:CD14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000170458.13
HGNCiHGNC:1628 CD14
MIMi158120 gene
neXtProtiNX_P08571

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi929
OpenTargetsiENSG00000170458
PharmGKBiPA26188

Chemistry databases

ChEMBLiCHEMBL2384897

Polymorphism and mutation databases

BioMutaiCD14
DMDMi20141203

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002088420 – 367Monocyte differentiation antigen CD14, urinary formAdd BLAST348
ChainiPRO_000002088520 – 345Monocyte differentiation antigen CD14, membrane-bound formAdd BLAST326
PropeptideiPRO_0000020886346 – 375Removed in mature formSequence analysisAdd BLAST30

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi25 ↔ 361 Publication
Disulfide bondi34 ↔ 511 Publication
Glycosylationi37N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi151N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi187 ↔ 2171 Publication
Disulfide bondi241 ↔ 2721 Publication
Glycosylationi282N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi323N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi336O-linked (GalNAc...) threonine1 Publication1
Lipidationi345GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.3 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP08571
MaxQBiP08571
PaxDbiP08571
PeptideAtlasiP08571
PRIDEiP08571
ProteomicsDBi52122

PTM databases

GlyConnecti774
iPTMnetiP08571
PhosphoSitePlusiP08571

Miscellaneous databases

PMAP-CutDBiP08571

Expressioni

Tissue specificityi

Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.2 Publications

Inductioni

The expression in monocytes is highly induced by 27-hydroxycholesterol, priming monocytes/macrophages such that LPS-mediated inflammatory reaction is accelerated. Secretion of soluble CD14 is also enhanced.1 Publication

Gene expression databases

BgeeiENSG00000170458 Expressed in 214 organ(s), highest expression level in liver
CleanExiHS_CD14
ExpressionAtlasiP08571 baseline and differential
GenevisibleiP08571 HS

Organism-specific databases

HPAiCAB033631
CAB072865
CAB072866
HPA001887
HPA002127

Interactioni

Subunit structurei

Interacts with LPS-bound LPB (PubMed:1698311, PubMed:23264655). Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (PubMed:11274165). Interacts with LPAR1 (By similarity). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively (PubMed:16880211). Interacts with MYO18A (PubMed:25965346).By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FSTL1Q128413EBI-3905196,EBI-2349801

Protein-protein interaction databases

BioGridi107367, 35 interactors
DIPiDIP-1030N
IntActiP08571, 27 interactors
MINTiP08571
STRINGi9606.ENSP00000304236

Structurei

3D structure databases

ProteinModelPortaliP08571
SMRiP08571
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati54 – 82LRR 1Add BLAST29
Repeati83 – 118LRR 2Add BLAST36
Repeati119 – 144LRR 3Add BLAST26
Repeati145 – 172LRR 4Add BLAST28
Repeati173 – 196LRR 5Add BLAST24
Repeati197 – 224LRR 6Add BLAST28
Repeati225 – 251LRR 7Add BLAST27
Repeati252 – 278LRR 8Add BLAST27
Repeati279 – 299LRR 9Add BLAST21
Repeati300 – 321LRR 10Add BLAST22
Repeati322 – 349LRR 11Add BLAST28

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni290 – 375Required for response to bacterial lipopolysaccharide (LPS)By similarityAdd BLAST86

Domaini

The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS.By similarity

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IXG9 Eukaryota
ENOG41118N2 LUCA
GeneTreeiENSGT00390000005689
HOGENOMiHOG000237268
HOVERGENiHBG005269
InParanoidiP08571
KOiK04391
OMAiLCPHKFP
OrthoDBiEOG091G0DA3
PhylomeDBiP08571
TreeFamiTF338550

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR016337 Monocyte_diff_Ag_CD14
PANTHERiPTHR10630 PTHR10630, 1 hit
PIRSFiPIRSF002017 CD14, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P08571-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERASCLLLL LLPLVHVSAT TPEPCELDDE DFRCVCNFSE PQPDWSEAFQ
60 70 80 90 100
CVSAVEVEIH AGGLNLEPFL KRVDADADPR QYADTVKALR VRRLTVGAAQ
110 120 130 140 150
VPAQLLVGAL RVLAYSRLKE LTLEDLKITG TMPPLPLEAT GLALSSLRLR
160 170 180 190 200
NVSWATGRSW LAELQQWLKP GLKVLSIAQA HSPAFSCEQV RAFPALTSLD
210 220 230 240 250
LSDNPGLGER GLMAALCPHK FPAIQNLALR NTGMETPTGV CAALAAAGVQ
260 270 280 290 300
PHSLDLSHNS LRATVNPSAP RCMWSSALNS LNLSFAGLEQ VPKGLPAKLR
310 320 330 340 350
VLDLSCNRLN RAPQPDELPE VDNLTLDGNP FLVPGTALPH EGSMNSGVVP
360 370
ACARSTLSVG VSGTLVLLQG ARGFA
Length:375
Mass (Da):40,076
Last modified:March 27, 2002 - v2
Checksum:i1746CDB41F394F8D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RFL4D6RFL4_HUMAN
Monocyte differentiation antigen CD...
CD14
213Annotation score:
D6RD81D6RD81_HUMAN
Monocyte differentiation antigen CD...
CD14
55Annotation score:
E7EVL5E7EVL5_HUMAN
Monocyte differentiation antigen CD...
CD14
81Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187C → Y in CAA29999 (PubMed:2453848).Curated1
Sequence conflicti303D → E in AAC83816 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024302204N → D. Corresponds to variant dbSNP:rs2228049Ensembl.1
Natural variantiVAR_050771341E → K. Corresponds to variant dbSNP:rs11556179Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06882 Genomic DNA Translation: CAA29999.1
X13334 mRNA Translation: CAA31711.1
M86511 mRNA Translation: AAA51930.1
AF097942 mRNA Translation: AAC83816.1
AB446505 mRNA Translation: BAG55282.1
BT007331 mRNA Translation: AAP35995.1
CH471062 Genomic DNA Translation: EAW62037.1
BC010507 mRNA Translation: AAH10507.1
AY044269 mRNA Translation: AAL02401.1
CCDSiCCDS4232.1
PIRiA27637 TDHUM4
RefSeqiNP_000582.1, NM_000591.3
NP_001035110.1, NM_001040021.2
NP_001167575.1, NM_001174104.1
NP_001167576.1, NM_001174105.1
UniGeneiHs.163867

Genome annotation databases

EnsembliENST00000302014; ENSP00000304236; ENSG00000170458
ENST00000401743; ENSP00000385519; ENSG00000170458
GeneIDi929
KEGGihsa:929
UCSCiuc003lgi.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

CD14 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06882 Genomic DNA Translation: CAA29999.1
X13334 mRNA Translation: CAA31711.1
M86511 mRNA Translation: AAA51930.1
AF097942 mRNA Translation: AAC83816.1
AB446505 mRNA Translation: BAG55282.1
BT007331 mRNA Translation: AAP35995.1
CH471062 Genomic DNA Translation: EAW62037.1
BC010507 mRNA Translation: AAH10507.1
AY044269 mRNA Translation: AAL02401.1
CCDSiCCDS4232.1
PIRiA27637 TDHUM4
RefSeqiNP_000582.1, NM_000591.3
NP_001035110.1, NM_001040021.2
NP_001167575.1, NM_001174104.1
NP_001167576.1, NM_001174105.1
UniGeneiHs.163867

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GLPX-ray4.00A26-335[»]
ProteinModelPortaliP08571
SMRiP08571
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107367, 35 interactors
DIPiDIP-1030N
IntActiP08571, 27 interactors
MINTiP08571
STRINGi9606.ENSP00000304236

Chemistry databases

ChEMBLiCHEMBL2384897

PTM databases

GlyConnecti774
iPTMnetiP08571
PhosphoSitePlusiP08571

Polymorphism and mutation databases

BioMutaiCD14
DMDMi20141203

Proteomic databases

EPDiP08571
MaxQBiP08571
PaxDbiP08571
PeptideAtlasiP08571
PRIDEiP08571
ProteomicsDBi52122

Protocols and materials databases

DNASUi929
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302014; ENSP00000304236; ENSG00000170458
ENST00000401743; ENSP00000385519; ENSG00000170458
GeneIDi929
KEGGihsa:929
UCSCiuc003lgi.3 human

Organism-specific databases

CTDi929
DisGeNETi929
EuPathDBiHostDB:ENSG00000170458.13
GeneCardsiCD14
HGNCiHGNC:1628 CD14
HPAiCAB033631
CAB072865
CAB072866
HPA001887
HPA002127
MIMi158120 gene
neXtProtiNX_P08571
OpenTargetsiENSG00000170458
PharmGKBiPA26188
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXG9 Eukaryota
ENOG41118N2 LUCA
GeneTreeiENSGT00390000005689
HOGENOMiHOG000237268
HOVERGENiHBG005269
InParanoidiP08571
KOiK04391
OMAiLCPHKFP
OrthoDBiEOG091G0DA3
PhylomeDBiP08571
TreeFamiTF338550

Enzyme and pathway databases

ReactomeiR-HSA-1236974 ER-Phagosome pathway
R-HSA-140534 via Death Receptors in the presence of ligand
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade
R-HSA-166020 Transfer of LPS from LBP carrier to CD14
R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane
R-HSA-166166 MyD88-independent TLR4 cascade
R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade
R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade
R-HSA-2562578 TRIF-mediated programmed cell death
R-HSA-5602498 MyD88 deficiency (TLR2/4)
R-HSA-5603041 IRAK4 deficiency (TLR2/4)
R-HSA-5686938 Regulation of TLR by endogenous ligand
R-HSA-6798695 Neutrophil degranulation
R-HSA-9014325 TICAM1,TRAF6-dependent induction of TAK1 complex
R-HSA-936964 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
R-HSA-937041 IKK complex recruitment mediated by RIP1
R-HSA-937072 TRAF6-mediated induction of TAK1 complex within TLR4 complex
R-HSA-975163 IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation
SIGNORiP08571

Miscellaneous databases

ChiTaRSiCD14 human
GeneWikiiCD14
GenomeRNAii929
PMAP-CutDBiP08571
PROiPR:P08571
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170458 Expressed in 214 organ(s), highest expression level in liver
CleanExiHS_CD14
ExpressionAtlasiP08571 baseline and differential
GenevisibleiP08571 HS

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR016337 Monocyte_diff_Ag_CD14
PANTHERiPTHR10630 PTHR10630, 1 hit
PIRSFiPIRSF002017 CD14, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCD14_HUMAN
AccessioniPrimary (citable) accession number: P08571
Secondary accession number(s): Q53XT5
, Q96FR6, Q96L99, Q9UNS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: March 27, 2002
Last modified: September 12, 2018
This is version 193 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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